BRENDA - Enzyme Database show
show all sequences of 1.1.1.76

Structural basis for chiral substrate recognition by two 2,3-butanediol dehydrogenases

Otagiri, M.; Ui, S.; Takusagawa, Y.; Ohtsuki, T.; Kurisu, G.; Kusunoki, M.; FEBS Lett. 584, 219-223 (2010)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
in complex with NAD+ and 2-mercaptoethanol, to 2.0 A resolution. The L-BDH subunit is composed of seven beta-strands, six alpha-helices and two short a helices, aFG1 and aFG2, which form a small lobe on top of the core domain. The conserved active site residues Asn112, Ser141, Tyr154 and Lys158 are located near the NAD+ molecule
Corynebacterium glutamicum
Engineering
Amino acid exchange
Commentary
Organism
F148N
trace activity below 0.1 U/mg
Corynebacterium glutamicum
I142Q
trace activity below 0.1 U/mg
Corynebacterium glutamicum
I142Q/F148N
trace activity below 0.1 U/mg
Corynebacterium glutamicum
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
competitive. In crystal strucuture, the mercaptoethanol molecule is located in the catalytic cleft formed by residues Ser141, Ileu142, Ala143, Phe148, Tyr154, Pro184, Gly185, Ileu186, Met191, Trp192 and Ile195. These residues may also be involved in substrate recognition and in stereospecific redox reactions
Corynebacterium glutamicum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Corynebacterium glutamicum
Q9ZNN8
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.1
-
substrate meso-2,3-butanediol, pH 8.0, 37C
Corynebacterium glutamicum
4.3
-
substrate (2S,3S)-2,3-butanediol, pH 8.0, 37C
Corynebacterium glutamicum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S,3S)-2,3-butanediol + NAD+
-
712019
Corynebacterium glutamicum
(3S)-acetoin + NADH + H+
-
-
-
?
meso-2,3-butanediol + NAD+
poor substrate
712019
Corynebacterium glutamicum
acetoin + NADH
-
-
-
?
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.3
-
2-mercaptoethanol
pH 8.0, 37C
Corynebacterium glutamicum
Crystallization (Commentary) (protein specific)
Crystallization
Organism
in complex with NAD+ and 2-mercaptoethanol, to 2.0 A resolution. The L-BDH subunit is composed of seven beta-strands, six alpha-helices and two short a helices, aFG1 and aFG2, which form a small lobe on top of the core domain. The conserved active site residues Asn112, Ser141, Tyr154 and Lys158 are located near the NAD+ molecule
Corynebacterium glutamicum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
F148N
trace activity below 0.1 U/mg
Corynebacterium glutamicum
I142Q
trace activity below 0.1 U/mg
Corynebacterium glutamicum
I142Q/F148N
trace activity below 0.1 U/mg
Corynebacterium glutamicum
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
competitive. In crystal strucuture, the mercaptoethanol molecule is located in the catalytic cleft formed by residues Ser141, Ileu142, Ala143, Phe148, Tyr154, Pro184, Gly185, Ileu186, Met191, Trp192 and Ile195. These residues may also be involved in substrate recognition and in stereospecific redox reactions
Corynebacterium glutamicum
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.3
-
2-mercaptoethanol
pH 8.0, 37C
Corynebacterium glutamicum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.1
-
substrate meso-2,3-butanediol, pH 8.0, 37C
Corynebacterium glutamicum
4.3
-
substrate (2S,3S)-2,3-butanediol, pH 8.0, 37C
Corynebacterium glutamicum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S,3S)-2,3-butanediol + NAD+
-
712019
Corynebacterium glutamicum
(3S)-acetoin + NADH + H+
-
-
-
?
meso-2,3-butanediol + NAD+
poor substrate
712019
Corynebacterium glutamicum
acetoin + NADH
-
-
-
?
Other publictions for EC 1.1.1.76
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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Xu
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1
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8
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721396
Wang
Characterization of a stereosp ...
Rhodococcus erythropolis, Rhodococcus erythropolis WZ010
Appl. Microbiol. Biotechnol.
98
641-650
2014
-
1
1
-
-
-
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1
1
1
5
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1
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2
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18
1
2
1
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2
1
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1
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1
1
1
-
-
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1
1
1
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1
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2
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18
1
2
1
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2
1
-
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1
1
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-
-
737381
Shimegi
Crystallization and preliminar ...
Corynebacterium glutamicum
Acta Crystallogr. Sect. F
70
461-463
2014
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-
1
1
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-
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-
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1
1
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1
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1
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1
1
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1
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1
1
1
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1
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1
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1
1
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-
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-
737865
Takeda
Identification and characteriz ...
Mycobacterium sp., Mycobacterium sp. B-009
Biosci. Biotechnol. Biochem.
78
1879-1886
2014
-
-
-
-
-
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-
1
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1
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3
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1
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1
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1
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1
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4
1
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1
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2
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1
1
739652
Wang
Engineering of cofactor regene ...
Corynebacterium glutamicum
Sci. Rep.
3
2643
2013
-
-
1
-
-
-
-
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1
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1
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1
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3
1
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1
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1
1
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1
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1
-
3
1
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-
-
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-
-
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-
710944
Gonzalez
Role of Saccharomyces cerevisi ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
76
670-679
2010
-
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1
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1
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1
1
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-
712019
Otagiri
Structural basis for chiral su ...
Corynebacterium glutamicum
FEBS Lett.
584
219-223
2010
-
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1
3
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1
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1
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2
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2
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3
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2
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2
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389578
Ui
Stereochemical applications of ...
Corynebacterium glutamicum, Corynebacterium glutamicum C-1012
Lett. Appl. Microbiol.
32
93-98
2001
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1
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9
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4
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6
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2
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1
2
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4
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6
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389579
Otagiri
-
Crystallization and preliminar ...
Corynebacterium glutamicum, Corynebacterium glutamicum C-1012
Protein Pept. Lett.
8
57-61
2001
-
-
1
1
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6
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2
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1
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1
1
1
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2
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389580
Takusagawa
Purification and characterizat ...
Corynebacterium glutamicum, Corynebacterium glutamicum C-1012
Biosci. Biotechnol. Biochem.
65
1876-1878
2001
1
1
1
-
-
-
9
5
-
6
3
-
-
8
-
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1
-
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-
2
-
12
1
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-
2
-
2
-
1
2
1
1
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1
1
1
2
-
-
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-
9
1
5
-
6
3
-
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1
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2
-
12
1
-
-
2
-
2
-
1
1
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-
389573
Ui
-
Mechanism for the formation of ...
Klebsiella pneumoniae, Klebsiella pneumoniae IAM 1063
J. Ferment. Technol.
62
551-559
1984
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1
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389574
Ui
Separation and qunatitation of ...
Corynebacterium glutamicum, Corynebacterium glutamicum C-1012
Agric. Biol. Chem.
48
2837-2838
1984
-
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1
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6
-
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1
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1
1
2
-
2
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1
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2
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2
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1
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1
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1
1
2
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2
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1
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-
-
-
389575
Ui
-
Laboratory-scale production of ...
Corynebacterium glutamicum, Corynebacterium glutamicum C-1012, Klebsiella pneumoniae, Klebsiella pneumoniae IAM 1063, no activity in Pseudomonas sp., no activity in Pseudomonas sp. s4, Paenibacillus polymyxa, Paenibacillus polymyxa IAM 1189, Saccharomyces cerevisiae, Saccharomyces cerevisiae OC-2, Serratia marcescens, Serratia marcescens IAM 1022
J. Ferment. Technol.
62
151-156
1984
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1
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18
-
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2
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4
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1
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2
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4
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4
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2
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4
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2
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389576
Voloch
Reduction of acetoin to 2,3-bu ...
Klebsiella pneumoniae
Biotechnol. Bioeng.
25
173-183
1983
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1
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389577
Taylor
Stereoisomeric specificities o ...
Aeromonas hydrophila, Bacillus subtilis, Bacillus subtilis Ford, Enterobacter aerogenes, Paenibacillus polymyxa
Biochim. Biophys. Acta
39
448-457
1960
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