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Literature summary for 1.1.1.76 extracted from

  • Otagiri, M.; Ui, S.; Takusagawa, Y.; Ohtsuki, T.; Kurisu, G.; Kusunoki, M.
    Structural basis for chiral substrate recognition by two 2,3-butanediol dehydrogenases (2010), FEBS Lett., 584, 219-223.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with NAD+ and 2-mercaptoethanol, to 2.0 A resolution. The L-BDH subunit is composed of seven beta-strands, six alpha-helices and two short a helices, aFG1 and aFG2, which form a small lobe on top of the core domain. The conserved active site residues Asn112, Ser141, Tyr154 and Lys158 are located near the NAD+ molecule Corynebacterium glutamicum

Protein Variants

Protein Variants Comment Organism
F148N trace activity below 0.1 U/mg Corynebacterium glutamicum
I142Q trace activity below 0.1 U/mg Corynebacterium glutamicum
I142Q/F148N trace activity below 0.1 U/mg Corynebacterium glutamicum

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol competitive. In crystal structure, the mercaptoethanol molecule is located in the catalytic cleft formed by residues Ser141, Ileu142, Ala143, Phe148, Tyr154, Pro184, Gly185, Ileu186, Met191, Trp192 and Ile195. These residues may also be involved in substrate recognition and in stereospecific redox reactions Corynebacterium glutamicum

Organism

Organism UniProt Comment Textmining
Corynebacterium glutamicum Q9ZNN8
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.1
-
substrate meso-2,3-butanediol, pH 8.0, 37°C Corynebacterium glutamicum
4.3
-
substrate (2S,3S)-2,3-butanediol, pH 8.0, 37°C Corynebacterium glutamicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2S,3S)-2,3-butanediol + NAD+
-
Corynebacterium glutamicum (3S)-acetoin + NADH + H+
-
?
meso-2,3-butanediol + NAD+ poor substrate Corynebacterium glutamicum acetoin + NADH
-
?

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.3
-
2-mercaptoethanol pH 8.0, 37°C Corynebacterium glutamicum