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show all sequences of 1.1.1.75

Cloning and expression of the L-1-amino-2-propanol dehydrogenase gene from Rhodococcus erythropolis, and its application to double chiral compound production

Kataoka, M.; Ishige, T.; Urano, N.; Nakamura, Y.; Sakuradani, E.; Fukui, S.; Kita, S.; Sakamoto, K.; Shimizu, S.; Appl. Microbiol. Biotechnol. 80, 597-604 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene aadh, DNA and amino acid sequence determination and analysis, expression in Escherichia coli JM109 leading to a 10.4fold increase in specific activity as to catalysis of the reduction of (S)-1-phenyl-2-methylaminopropan-1-one, as compared with that of Rhodococcus erythropolis strain MAK154 induced by 1-amino-2-propanol. Coexpression of aadh with glucose dehydrogenase gene gdh as cofactor regeneration enzyme, and installation of a system for sufficient production of d-psi-pseudoephedrine from racemic (S)-1-phenyl-2-methylaminopropan-1-one
Rhodococcus erythropolis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-1-amino-2-propanol + NADP+
Rhodococcus erythropolis
-
aminoacetone + NADPH + H+
-
-
?
L-1-amino-2-propanol + NADP+
Rhodococcus erythropolis MAK154
-
aminoacetone + NADPH + H+
-
-
?
additional information
Rhodococcus erythropolis
the enzyme catalyzes the stereospecific dehydrogenation of several aminoalcohols and also catalyzes the NADPH-dependent asymmetric reduction of (S)-1-phenyl-2-methylaminopropan-1-one to d-psi-EP
?
-
-
-
additional information
Rhodococcus erythropolis MAK154
the enzyme catalyzes the stereospecific dehydrogenation of several aminoalcohols and also catalyzes the NADPH-dependent asymmetric reduction of (S)-1-phenyl-2-methylaminopropan-1-one to d-psi-EP
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus erythropolis
A1IG83
strain MAK154, gene aadh
-
Rhodococcus erythropolis MAK154
A1IG83
strain MAK154, gene aadh
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-aminocyclohexanol + NADP+
-
695805
Rhodococcus erythropolis
2-aminocyclohexanone + NADPH + H+
-
-
-
?
D-glucose + NADP+
-
695805
Rhodococcus erythropolis
? + NADPH + H+
-
-
-
?
D-glucose + NADP+
-
695805
Rhodococcus erythropolis MAK154
? + NADPH + H+
-
-
-
?
L-1-amino-2-butanol + NADP+
-
695805
Rhodococcus erythropolis
1-amino-2-butanone + NADPH + H+
-
-
-
?
L-1-amino-2-butanol + NADP+
-
695805
Rhodococcus erythropolis MAK154
1-amino-2-butanone + NADPH + H+
-
-
-
?
L-1-amino-2-propanol + NADP+
-
695805
Rhodococcus erythropolis
aminoacetone + NADPH + H+
-
-
-
?
L-1-amino-2-propanol + NADP+
-
695805
Rhodococcus erythropolis MAK154
aminoacetone + NADPH + H+
-
-
-
?
additional information
the enzyme catalyzes the stereospecific dehydrogenation of several aminoalcohols and also catalyzes the NADPH-dependent asymmetric reduction of (S)-1-phenyl-2-methylaminopropan-1-one to d-psi-EP
695805
Rhodococcus erythropolis
?
-
-
-
-
additional information
substrate specificity of AADH and optimization of the catalytic reaction assay method, overview
695805
Rhodococcus erythropolis
?
-
-
-
-
additional information
the enzyme catalyzes the stereospecific dehydrogenation of several aminoalcohols and also catalyzes the NADPH-dependent asymmetric reduction of (S)-1-phenyl-2-methylaminopropan-1-one to d-psi-EP
695805
Rhodococcus erythropolis MAK154
?
-
-
-
-
additional information
substrate specificity of AADH and optimization of the catalytic reaction assay method, overview
695805
Rhodococcus erythropolis MAK154
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rhodococcus erythropolis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Rhodococcus erythropolis
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
dependent on
Rhodococcus erythropolis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene aadh, DNA and amino acid sequence determination and analysis, expression in Escherichia coli JM109 leading to a 10.4fold increase in specific activity as to catalysis of the reduction of (S)-1-phenyl-2-methylaminopropan-1-one, as compared with that of Rhodococcus erythropolis strain MAK154 induced by 1-amino-2-propanol. Coexpression of aadh with glucose dehydrogenase gene gdh as cofactor regeneration enzyme, and installation of a system for sufficient production of d-psi-pseudoephedrine from racemic (S)-1-phenyl-2-methylaminopropan-1-one
Rhodococcus erythropolis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
dependent on
Rhodococcus erythropolis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-1-amino-2-propanol + NADP+
Rhodococcus erythropolis
-
aminoacetone + NADPH + H+
-
-
?
L-1-amino-2-propanol + NADP+
Rhodococcus erythropolis MAK154
-
aminoacetone + NADPH + H+
-
-
?
additional information
Rhodococcus erythropolis
the enzyme catalyzes the stereospecific dehydrogenation of several aminoalcohols and also catalyzes the NADPH-dependent asymmetric reduction of (S)-1-phenyl-2-methylaminopropan-1-one to d-psi-EP
?
-
-
-
additional information
Rhodococcus erythropolis MAK154
the enzyme catalyzes the stereospecific dehydrogenation of several aminoalcohols and also catalyzes the NADPH-dependent asymmetric reduction of (S)-1-phenyl-2-methylaminopropan-1-one to d-psi-EP
?
-
-
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-aminocyclohexanol + NADP+
-
695805
Rhodococcus erythropolis
2-aminocyclohexanone + NADPH + H+
-
-
-
?
D-glucose + NADP+
-
695805
Rhodococcus erythropolis
? + NADPH + H+
-
-
-
?
D-glucose + NADP+
-
695805
Rhodococcus erythropolis MAK154
? + NADPH + H+
-
-
-
?
L-1-amino-2-butanol + NADP+
-
695805
Rhodococcus erythropolis
1-amino-2-butanone + NADPH + H+
-
-
-
?
L-1-amino-2-butanol + NADP+
-
695805
Rhodococcus erythropolis MAK154
1-amino-2-butanone + NADPH + H+
-
-
-
?
L-1-amino-2-propanol + NADP+
-
695805
Rhodococcus erythropolis
aminoacetone + NADPH + H+
-
-
-
?
L-1-amino-2-propanol + NADP+
-
695805
Rhodococcus erythropolis MAK154
aminoacetone + NADPH + H+
-
-
-
?
additional information
the enzyme catalyzes the stereospecific dehydrogenation of several aminoalcohols and also catalyzes the NADPH-dependent asymmetric reduction of (S)-1-phenyl-2-methylaminopropan-1-one to d-psi-EP
695805
Rhodococcus erythropolis
?
-
-
-
-
additional information
substrate specificity of AADH and optimization of the catalytic reaction assay method, overview
695805
Rhodococcus erythropolis
?
-
-
-
-
additional information
the enzyme catalyzes the stereospecific dehydrogenation of several aminoalcohols and also catalyzes the NADPH-dependent asymmetric reduction of (S)-1-phenyl-2-methylaminopropan-1-one to d-psi-EP
695805
Rhodococcus erythropolis MAK154
?
-
-
-
-
additional information
substrate specificity of AADH and optimization of the catalytic reaction assay method, overview
695805
Rhodococcus erythropolis MAK154
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Rhodococcus erythropolis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Rhodococcus erythropolis
Other publictions for EC 1.1.1.75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
695805
Kataoka
Cloning and expression of the ...
Rhodococcus erythropolis, Rhodococcus erythropolis MAK154
Appl. Microbiol. Biotechnol.
80
597-604
2008
-
-
1
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
11
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
287303
Faulkner
Microbial metabolism of amino ...
Pseudomonas sp.
Biochem. J.
138
263-276
1974
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287302
Willetts
Threonine metabolism in a stra ...
Bacillus subtilis
Biochim. Biophys. Acta
252
98-104
1971
-
-
-
-
-
-
-
2
-
1
1
1
-
1
-
-
1
-
-
-
1
2
9
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
1
1
-
-
-
1
-
-
1
2
9
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
287300
Cox
Aminopropanol dehydrogenase ac ...
Rattus norvegicus
Biochim. Biophys. Acta
170
438-439
1968
-
-
-
-
-
-
3
3
1
-
-
1
-
1
-
-
-
-
-
1
1
1
3
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
1
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
287304
Pickard
Purification and properties of ...
Pseudomonas sp.
J. Gen. Microbiol.
54
115-126
1968
-
-
-
-
-
1
1
3
-
-
1
1
-
1
-
-
1
-
-
-
3
1
9
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
3
-
-
1
1
-
-
-
1
-
-
3
1
9
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287299
Turner
Microbial metabolism of amino ...
Escherichia coli
Biochem. J.
104
112-121
1967
-
-
-
-
-
-
9
4
-
-
-
1
-
1
-
-
1
-
-
-
1
2
6
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
9
-
4
-
-
-
1
-
-
-
1
-
-
1
2
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287301
Turner
Amino ketone formation and ami ...
Rattus norvegicus
Biochem. J.
102
511-519
1967
-
-
-
-
-
1
4
3
1
2
-
1
-
1
-
-
-
-
-
5
1
-
4
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
3
1
2
-
1
-
-
-
-
-
5
1
-
4
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287298
Turner
Microbial metabolism of amino ...
Escherichia coli
Biochem. J.
99
427-433
1966
-
-
-
-
-
-
2
2
-
3
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
3
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287305
Pickard
-
Microbial metabolism of 1-amin ...
Pseudomonas sp.
J. Gen. Microbiol.
45
i-ii
1966
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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-
-
-
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3
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1
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-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287306
Higgins
-
Microbial metabolism of aminoa ...
Pseudomonas sp.
Biochem. J.
99
26P-27P
1966
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
-
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1
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1
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1
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1
-
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287307
Higgins
-
1-Aminopropan-2-ol-NAD oxidore ...
Escherichia coli, Rattus norvegicus
Biochem. J.
99
27P
1966
-
-
-
-
-
-
-
-
1
2
-
2
-
2
-
-
-
-
-
1
-
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5
-
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3
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1
2
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2
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1
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5
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3
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