BRENDA - Enzyme Database show
show all sequences of 1.1.1.75

Purification and properties of l-1-aminopropan-2-ol. NAD oxidoreductase from a pseudomonad grown on DL-1-aminopropan-2-ol

Pickard, M.A.; Higgins, I.J.; Turner, J.M.; J. Gen. Microbiol. 54, 115-126 (1968)

Data extracted from this reference:

General Stability
General Stability
Organism
dialysis overnight at 4C, no loss of activity
Pseudomonas sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
D-1-amininopropan-2-ol
competitive
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
D,L-1-aminopropan-2-ol
-
Pseudomonas sp.
0.2
-
NADP+
with D,L-1-aminopropan-2-ol as substrate
Pseudomonas sp.
0.3
-
NAD+
with D,L-1-aminopropan-2-ol as substrate
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70000
80000
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-1-aminopropan-2-ol + NAD+
Pseudomonas sp.
involved in L-threonine and vitamin B12 pathway
aminoacetone + NADH
-
Pseudomonas sp.
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.001
-
with 1-aminopropanol as growth substrate
Pseudomonas sp.
0.4
-
crude extract
Pseudomonas sp.
8.5
-
purified enzyme
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
-15C phosphate buffer, pH 7.2, 1 mg protein/ml, stable for more than 1 year
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,3-diaminopropan-2-ol + NAD+
poor substrate
287304
Pseudomonas sp.
2-aminoacetamide + NADH
-
-
-
?
D,L-1-aminopropan-2-ol + NAD+
-
287304
Pseudomonas sp.
aminoacetone + NADH
-
-
-
-
D,L-2-hydroxy-2-phenylethylamine + NAD+
-
287304
Pseudomonas sp.
2-amino-1-phenylethanone + NADH
-
-
-
?
D,L-3-hydroxybutyrate + NAD+
-
287304
Pseudomonas sp.
?
-
-
-
?
D,L-aminobutan-2-ol + NAD+
-
287304
Pseudomonas sp.
1-amino-2-oxobutane + NADH
-
-
-
?
D,L-phenylserine + NAD+
-
287304
Pseudomonas sp.
?
-
-
-
?
L-1-aminopropan-2-ol + NAD+
no activity with D-enantiomer
287304
Pseudomonas sp.
aminoacetone + NADH
-
287304
Pseudomonas sp.
r
L-1-aminopropan-2-ol + NAD+
involved in L-threonine and vitamin B12 pathway
287304
Pseudomonas sp.
aminoacetone + NADH
-
287304
Pseudomonas sp.
r
L-1-aminopropan-2-ol + NADP+
about the same activity as with NAD+ as cofactor
287304
Pseudomonas sp.
aminoacetone + NADPH
-
287304
Pseudomonas sp.
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Pseudomonas sp.
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10.5
low activity at pH 6, 80% of activity at pH 10.5
Pseudomonas sp.
General Stability (protein specific)
General Stability
Organism
dialysis overnight at 4C, no loss of activity
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-1-amininopropan-2-ol
competitive
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.12
-
D,L-1-aminopropan-2-ol
-
Pseudomonas sp.
0.2
-
NADP+
with D,L-1-aminopropan-2-ol as substrate
Pseudomonas sp.
0.3
-
NAD+
with D,L-1-aminopropan-2-ol as substrate
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
70000
80000
gel filtration
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-1-aminopropan-2-ol + NAD+
Pseudomonas sp.
involved in L-threonine and vitamin B12 pathway
aminoacetone + NADH
-
Pseudomonas sp.
r
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.001
-
with 1-aminopropanol as growth substrate
Pseudomonas sp.
0.4
-
crude extract
Pseudomonas sp.
8.5
-
purified enzyme
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
-15C phosphate buffer, pH 7.2, 1 mg protein/ml, stable for more than 1 year
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,3-diaminopropan-2-ol + NAD+
poor substrate
287304
Pseudomonas sp.
2-aminoacetamide + NADH
-
-
-
?
D,L-1-aminopropan-2-ol + NAD+
-
287304
Pseudomonas sp.
aminoacetone + NADH
-
-
-
-
D,L-2-hydroxy-2-phenylethylamine + NAD+
-
287304
Pseudomonas sp.
2-amino-1-phenylethanone + NADH
-
-
-
?
D,L-3-hydroxybutyrate + NAD+
-
287304
Pseudomonas sp.
?
-
-
-
?
D,L-aminobutan-2-ol + NAD+
-
287304
Pseudomonas sp.
1-amino-2-oxobutane + NADH
-
-
-
?
D,L-phenylserine + NAD+
-
287304
Pseudomonas sp.
?
-
-
-
?
L-1-aminopropan-2-ol + NAD+
no activity with D-enantiomer
287304
Pseudomonas sp.
aminoacetone + NADH
-
287304
Pseudomonas sp.
r
L-1-aminopropan-2-ol + NAD+
involved in L-threonine and vitamin B12 pathway
287304
Pseudomonas sp.
aminoacetone + NADH
-
287304
Pseudomonas sp.
r
L-1-aminopropan-2-ol + NADP+
about the same activity as with NAD+ as cofactor
287304
Pseudomonas sp.
aminoacetone + NADPH
-
287304
Pseudomonas sp.
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Pseudomonas sp.
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10.5
low activity at pH 6, 80% of activity at pH 10.5
Pseudomonas sp.
Other publictions for EC 1.1.1.75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
695805
Kataoka
Cloning and expression of the ...
Rhodococcus erythropolis, Rhodococcus erythropolis MAK154
Appl. Microbiol. Biotechnol.
80
597-604
2008
-
-
1
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
11
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
287303
Faulkner
Microbial metabolism of amino ...
Pseudomonas sp.
Biochem. J.
138
263-276
1974
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287302
Willetts
Threonine metabolism in a stra ...
Bacillus subtilis
Biochim. Biophys. Acta
252
98-104
1971
-
-
-
-
-
-
-
2
-
1
1
1
-
1
-
-
1
-
-
-
1
2
9
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
1
1
-
-
-
1
-
-
1
2
9
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
287300
Cox
Aminopropanol dehydrogenase ac ...
Rattus norvegicus
Biochim. Biophys. Acta
170
438-439
1968
-
-
-
-
-
-
3
3
1
-
-
1
-
1
-
-
-
-
-
1
1
1
3
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
1
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
287304
Pickard
Purification and properties of ...
Pseudomonas sp.
J. Gen. Microbiol.
54
115-126
1968
-
-
-
-
-
1
1
3
-
-
1
1
-
1
-
-
1
-
-
-
3
1
9
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
3
-
-
1
1
-
-
-
1
-
-
3
1
9
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287299
Turner
Microbial metabolism of amino ...
Escherichia coli
Biochem. J.
104
112-121
1967
-
-
-
-
-
-
9
4
-
-
-
1
-
1
-
-
1
-
-
-
1
2
6
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
9
-
4
-
-
-
1
-
-
-
1
-
-
1
2
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287301
Turner
Amino ketone formation and ami ...
Rattus norvegicus
Biochem. J.
102
511-519
1967
-
-
-
-
-
1
4
3
1
2
-
1
-
1
-
-
-
-
-
5
1
-
4
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
3
1
2
-
1
-
-
-
-
-
5
1
-
4
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287298
Turner
Microbial metabolism of amino ...
Escherichia coli
Biochem. J.
99
427-433
1966
-
-
-
-
-
-
2
2
-
3
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
3
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287305
Pickard
-
Microbial metabolism of 1-amin ...
Pseudomonas sp.
J. Gen. Microbiol.
45
i-ii
1966
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287306
Higgins
-
Microbial metabolism of aminoa ...
Pseudomonas sp.
Biochem. J.
99
26P-27P
1966
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287307
Higgins
-
1-Aminopropan-2-ol-NAD oxidore ...
Escherichia coli, Rattus norvegicus
Biochem. J.
99
27P
1966
-
-
-
-
-
-
-
-
1
2
-
2
-
2
-
-
-
-
-
1
-
-
5
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
2
-
-
-
-
-
1
-
-
5
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-