BRENDA - Enzyme Database show
show all sequences of 1.1.1.75

Threonine metabolism in a strain of Bacillus subtilis enzymic oxidation of 1-aminopropan-2-ol and aminoacetone

Willetts, A.J.; Turner, J.M.; Biochim. Biophys. Acta 252, 98-104 (1971)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
31
-
L-1-Aminopropan-2-ol
-
Bacillus subtilis
54
-
D-1-Aminopropan-2-ol
-
Bacillus subtilis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
stimulating at all concentrations
Bacillus subtilis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
250000
-
gel filtration
Bacillus subtilis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-1-aminopropan-2-ol + NAD+
Bacillus subtilis
-
aminoacetone + NADH
-
Bacillus subtilis
ir
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus subtilis
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Bacillus subtilis
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.001
-
-
Bacillus subtilis
Storage Stability
Storage Stability
Organism
-15°C, 0.1 M Tris/HCl buffer, pH 7.2, 50% loss of activity
Bacillus subtilis
0°C, 0.1 M Tris/HCl buffer, pH 7.2, 90% loss of activity
Bacillus subtilis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,3-diaminopropan-2-ol + NAD+
-
287302
Bacillus subtilis
2-aminoacetamide + NADH
-
-
-
?
1-amino-3-diethylaminopropan-2-ol + NAD+
-
287302
Bacillus subtilis
2-amino-N,N-diethylacetamide + NADH
-
-
-
?
1-aminopropan-2,3-diol + NAD+
-
287302
Bacillus subtilis
?
-
-
-
?
butan-2,3-diol + NAD+
-
287302
Bacillus subtilis
?
-
-
-
?
D,L-1-aminopropan-2-ol + NAD+
D-enantiomer 50% of activity with L-enantiomer
287302
Bacillus subtilis
aminoacetone + NADH
-
287302
Bacillus subtilis
ir
hydroxyacetone + NAD+
no activity with acetone
287302
Bacillus subtilis
2-oxopropanal + NADH
-
-
-
?
L-1-aminopropan-2-ol + NAD+
-
287302
Bacillus subtilis
aminoacetone + NADH
-
287302
Bacillus subtilis
ir
propan-1,2-diol + NAD+
-
287302
Bacillus subtilis
?
-
-
-
?
propan-1,3-diol + NAD+
-
287302
Bacillus subtilis
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Bacillus subtilis
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
60
-
Bacillus subtilis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
sharp optimum
Bacillus subtilis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
31
-
L-1-Aminopropan-2-ol
-
Bacillus subtilis
54
-
D-1-Aminopropan-2-ol
-
Bacillus subtilis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
stimulating at all concentrations
Bacillus subtilis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
250000
-
gel filtration
Bacillus subtilis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-1-aminopropan-2-ol + NAD+
Bacillus subtilis
-
aminoacetone + NADH
-
Bacillus subtilis
ir
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Bacillus subtilis
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.001
-
-
Bacillus subtilis
Storage Stability (protein specific)
Storage Stability
Organism
-15°C, 0.1 M Tris/HCl buffer, pH 7.2, 50% loss of activity
Bacillus subtilis
0°C, 0.1 M Tris/HCl buffer, pH 7.2, 90% loss of activity
Bacillus subtilis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1,3-diaminopropan-2-ol + NAD+
-
287302
Bacillus subtilis
2-aminoacetamide + NADH
-
-
-
?
1-amino-3-diethylaminopropan-2-ol + NAD+
-
287302
Bacillus subtilis
2-amino-N,N-diethylacetamide + NADH
-
-
-
?
1-aminopropan-2,3-diol + NAD+
-
287302
Bacillus subtilis
?
-
-
-
?
butan-2,3-diol + NAD+
-
287302
Bacillus subtilis
?
-
-
-
?
D,L-1-aminopropan-2-ol + NAD+
D-enantiomer 50% of activity with L-enantiomer
287302
Bacillus subtilis
aminoacetone + NADH
-
287302
Bacillus subtilis
ir
hydroxyacetone + NAD+
no activity with acetone
287302
Bacillus subtilis
2-oxopropanal + NADH
-
-
-
?
L-1-aminopropan-2-ol + NAD+
-
287302
Bacillus subtilis
aminoacetone + NADH
-
287302
Bacillus subtilis
ir
propan-1,2-diol + NAD+
-
287302
Bacillus subtilis
?
-
-
-
?
propan-1,3-diol + NAD+
-
287302
Bacillus subtilis
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Bacillus subtilis
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
20
60
-
Bacillus subtilis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.5
-
sharp optimum
Bacillus subtilis
Other publictions for EC 1.1.1.75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
695805
Kataoka
Cloning and expression of the ...
Rhodococcus erythropolis, Rhodococcus erythropolis MAK154
Appl. Microbiol. Biotechnol.
80
597-604
2008
-
-
1
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
11
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
287303
Faulkner
Microbial metabolism of amino ...
Pseudomonas sp.
Biochem. J.
138
263-276
1974
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287302
Willetts
Threonine metabolism in a stra ...
Bacillus subtilis
Biochim. Biophys. Acta
252
98-104
1971
-
-
-
-
-
-
-
2
-
1
1
1
-
1
-
-
1
-
-
-
1
2
9
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
1
1
-
-
-
1
-
-
1
2
9
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
287300
Cox
Aminopropanol dehydrogenase ac ...
Rattus norvegicus
Biochim. Biophys. Acta
170
438-439
1968
-
-
-
-
-
-
3
3
1
-
-
1
-
1
-
-
-
-
-
1
1
1
3
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
1
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
287304
Pickard
Purification and properties of ...
Pseudomonas sp.
J. Gen. Microbiol.
54
115-126
1968
-
-
-
-
-
1
1
3
-
-
1
1
-
1
-
-
1
-
-
-
3
1
9
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
3
-
-
1
1
-
-
-
1
-
-
3
1
9
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287299
Turner
Microbial metabolism of amino ...
Escherichia coli
Biochem. J.
104
112-121
1967
-
-
-
-
-
-
9
4
-
-
-
1
-
1
-
-
1
-
-
-
1
2
6
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
9
-
4
-
-
-
1
-
-
-
1
-
-
1
2
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287301
Turner
Amino ketone formation and ami ...
Rattus norvegicus
Biochem. J.
102
511-519
1967
-
-
-
-
-
1
4
3
1
2
-
1
-
1
-
-
-
-
-
5
1
-
4
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
3
1
2
-
1
-
-
-
-
-
5
1
-
4
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287298
Turner
Microbial metabolism of amino ...
Escherichia coli
Biochem. J.
99
427-433
1966
-
-
-
-
-
-
2
2
-
3
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
3
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287305
Pickard
-
Microbial metabolism of 1-amin ...
Pseudomonas sp.
J. Gen. Microbiol.
45
i-ii
1966
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287306
Higgins
-
Microbial metabolism of aminoa ...
Pseudomonas sp.
Biochem. J.
99
26P-27P
1966
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287307
Higgins
-
1-Aminopropan-2-ol-NAD oxidore ...
Escherichia coli, Rattus norvegicus
Biochem. J.
99
27P
1966
-
-
-
-
-
-
-
-
1
2
-
2
-
2
-
-
-
-
-
1
-
-
5
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
2
-
-
-
-
-
1
-
-
5
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-