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show all sequences of 1.1.1.75

Amino ketone formation and aminopropanol-dehydrogenase activity in rat-liver preparations

Turner, J.M.; Willetts, A.J.; Biochem. J. 102, 511-519 (1967)

Data extracted from this reference:

General Stability
General Stability
Organism
stable on freezing and thawing an ultrasonication
Rattus norvegicus
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Rattus norvegicus
D,L-3-hydroxybutyrate
non-competitive, Ki: 200 mM in liver homogenates, little effect in soluble fraction from disintegrated mitochondria
Rattus norvegicus
EDTA
weak inhibition at high concentration, little effect in soluble extract of disintegrated mitochondria
Rattus norvegicus
L-cysteine
-
Rattus norvegicus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
15
-
1-aminopropan-2-ol
liver homogenates
Rattus norvegicus
200
-
1-aminopropan-2-ol
soluble extract of disintegrated mitochondria
Rattus norvegicus
200
-
D,L-4-amino-3-hydroxybutyrate
liver homogenates
Rattus norvegicus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Rattus norvegicus
5739
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
inhibitory at all concentrations
Rattus norvegicus
Mg2+
slightly activating at 1.3 mM, inhibitory above 3 mM
Rattus norvegicus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-1-aminopropan-2-ol + NAD+
Rattus norvegicus
possible role in carnitine metabolism
aminoacetone + NADH
-
Rattus norvegicus
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rattus norvegicus
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
muscle
-
Rattus norvegicus
-
spleen
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.001
-
soluble extract of disintegrated mitochondria
Rattus norvegicus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D,L-1-aminopropan-2-ol + NAD+
-
287301
Rattus norvegicus
aminoacetone + NADH
-
287301
Rattus norvegicus
-
D,L-4-amino-3-hydroxybutyrate + NAD+
-
287301
Rattus norvegicus
4-amino-3-oxobutyrate + NADH
-
-
-
?
L-1-aminopropan-2-ol + NAD+
-
287301
Rattus norvegicus
aminoacetone + NADH
-
287301
Rattus norvegicus
-
L-1-aminopropan-2-ol + NAD+
possible role in carnitine metabolism
287301
Rattus norvegicus
aminoacetone + NADH
-
287301
Rattus norvegicus
-
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.1
-
soluble extract of disintegrated mitochondria
Rattus norvegicus
9.8
-
liver homogenates
Rattus norvegicus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.5
11
very low activity at pH 7.5, 60% of activity at pH 11 in liver homogenates
Rattus norvegicus
8.5
10.5
40% of activity in soluble extract of disintegrated mitochondria
Rattus norvegicus
General Stability (protein specific)
General Stability
Organism
stable on freezing and thawing an ultrasonication
Rattus norvegicus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-mercaptoethanol
-
Rattus norvegicus
D,L-3-hydroxybutyrate
non-competitive, Ki: 200 mM in liver homogenates, little effect in soluble fraction from disintegrated mitochondria
Rattus norvegicus
EDTA
weak inhibition at high concentration, little effect in soluble extract of disintegrated mitochondria
Rattus norvegicus
L-cysteine
-
Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
15
-
1-aminopropan-2-ol
liver homogenates
Rattus norvegicus
200
-
1-aminopropan-2-ol
soluble extract of disintegrated mitochondria
Rattus norvegicus
200
-
D,L-4-amino-3-hydroxybutyrate
liver homogenates
Rattus norvegicus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Rattus norvegicus
5739
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
inhibitory at all concentrations
Rattus norvegicus
Mg2+
slightly activating at 1.3 mM, inhibitory above 3 mM
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-1-aminopropan-2-ol + NAD+
Rattus norvegicus
possible role in carnitine metabolism
aminoacetone + NADH
-
Rattus norvegicus
-
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
heart
-
Rattus norvegicus
-
kidney
-
Rattus norvegicus
-
liver
-
Rattus norvegicus
-
muscle
-
Rattus norvegicus
-
spleen
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.001
-
soluble extract of disintegrated mitochondria
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D,L-1-aminopropan-2-ol + NAD+
-
287301
Rattus norvegicus
aminoacetone + NADH
-
287301
Rattus norvegicus
-
D,L-4-amino-3-hydroxybutyrate + NAD+
-
287301
Rattus norvegicus
4-amino-3-oxobutyrate + NADH
-
-
-
?
L-1-aminopropan-2-ol + NAD+
-
287301
Rattus norvegicus
aminoacetone + NADH
-
287301
Rattus norvegicus
-
L-1-aminopropan-2-ol + NAD+
possible role in carnitine metabolism
287301
Rattus norvegicus
aminoacetone + NADH
-
287301
Rattus norvegicus
-
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.1
-
soluble extract of disintegrated mitochondria
Rattus norvegicus
9.8
-
liver homogenates
Rattus norvegicus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.5
11
very low activity at pH 7.5, 60% of activity at pH 11 in liver homogenates
Rattus norvegicus
8.5
10.5
40% of activity in soluble extract of disintegrated mitochondria
Rattus norvegicus
Other publictions for EC 1.1.1.75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
695805
Kataoka
Cloning and expression of the ...
Rhodococcus erythropolis, Rhodococcus erythropolis MAK154
Appl. Microbiol. Biotechnol.
80
597-604
2008
-
-
1
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
11
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
287303
Faulkner
Microbial metabolism of amino ...
Pseudomonas sp.
Biochem. J.
138
263-276
1974
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287302
Willetts
Threonine metabolism in a stra ...
Bacillus subtilis
Biochim. Biophys. Acta
252
98-104
1971
-
-
-
-
-
-
-
2
-
1
1
1
-
1
-
-
1
-
-
-
1
2
9
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
1
1
-
-
-
1
-
-
1
2
9
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
287300
Cox
Aminopropanol dehydrogenase ac ...
Rattus norvegicus
Biochim. Biophys. Acta
170
438-439
1968
-
-
-
-
-
-
3
3
1
-
-
1
-
1
-
-
-
-
-
1
1
1
3
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
1
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
287304
Pickard
Purification and properties of ...
Pseudomonas sp.
J. Gen. Microbiol.
54
115-126
1968
-
-
-
-
-
1
1
3
-
-
1
1
-
1
-
-
1
-
-
-
3
1
9
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
3
-
-
1
1
-
-
-
1
-
-
3
1
9
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287299
Turner
Microbial metabolism of amino ...
Escherichia coli
Biochem. J.
104
112-121
1967
-
-
-
-
-
-
9
4
-
-
-
1
-
1
-
-
1
-
-
-
1
2
6
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
9
-
4
-
-
-
1
-
-
-
1
-
-
1
2
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287301
Turner
Amino ketone formation and ami ...
Rattus norvegicus
Biochem. J.
102
511-519
1967
-
-
-
-
-
1
4
3
1
2
-
1
-
1
-
-
-
-
-
5
1
-
4
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
3
1
2
-
1
-
-
-
-
-
5
1
-
4
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287298
Turner
Microbial metabolism of amino ...
Escherichia coli
Biochem. J.
99
427-433
1966
-
-
-
-
-
-
2
2
-
3
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
3
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287305
Pickard
-
Microbial metabolism of 1-amin ...
Pseudomonas sp.
J. Gen. Microbiol.
45
i-ii
1966
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
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-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287306
Higgins
-
Microbial metabolism of aminoa ...
Pseudomonas sp.
Biochem. J.
99
26P-27P
1966
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287307
Higgins
-
1-Aminopropan-2-ol-NAD oxidore ...
Escherichia coli, Rattus norvegicus
Biochem. J.
99
27P
1966
-
-
-
-
-
-
-
-
1
2
-
2
-
2
-
-
-
-
-
1
-
-
5
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
2
-
-
-
-
-
1
-
-
5
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-