BRENDA - Enzyme Database show
show all sequences of 1.1.1.75

Microbial metabolism of amino ketones. L-1-aminopropan-2-ol dehydrogenase and L-threonine dehydrgenase in Escerichia coli

Turner, J.M.; Biochem. J. 104, 112-121 (1967)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
3-chloromercuribenzoate
complete inhibition at 0.05 mM
Escherichia coli
8-hydroxyquinoline
nearly complete inhibition at 2.5 mM
Escherichia coli
D,L-1,3-diaminopropan-2-ol
-
Escherichia coli
D,L-2hydroxy-2-phenylethylamine
-
Escherichia coli
D,L-serine
-
Escherichia coli
D-1-amininopropan-2-ol
competitive
Escherichia coli
iodoacetate
more inhibitory in intact cells than in cell free extracts
Escherichia coli
L-1-(3,4-dihydroxyphenyl)-2-aminoethanol
noradrenalin
Escherichia coli
sodium carbonate/dicarbonate buffers
at alkaline pH
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
NAD+
at pH 7 with aminopropanol as substrate
Escherichia coli
0.4
-
NAD+
at pH 9.6 with aminopropanol as substrate
Escherichia coli
1.5
-
L-1-Aminopropan-2-ol
cell free extracts
Escherichia coli
10
-
D,L-1-aminopropan-2-ol
cell free extracts
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-1-aminopropan-2-ol + NAD+
Escherichia coli
involved in L-threonine and vitamin B12 pathway
aminoacetone + NADH
-
Escherichia coli
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.008
-
cell free extract
Escherichia coli
Storage Stability
Storage Stability
Organism
-15C stable for several days
Escherichia coli
0-5C significant loss in activity
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D,L-1-aminopropan-2-ol + NAD+
more active with L-enantiomer, activity with D-enantiomer might be due to D-aminopropanol specific enzyme
287299
Escherichia coli
aminoacetone + NADH
-
287299
Escherichia coli
?
D,L-aminobutan-2-ol + NAD+
-
287299
Escherichia coli
1-amino-2-oxobutane + NADH
-
-
-
?
D,L-phenylserine + NAD+
-
287299
Escherichia coli
?
-
-
-
?
L-1-aminopropan-2-ol + NAD+
-
287299
Escherichia coli
aminoacetone + NADH
-
287299
Escherichia coli
?
L-1-aminopropan-2-ol + NAD+
involved in L-threonine and vitamin B12 pathway
287299
Escherichia coli
aminoacetone + NADH
-
287299
Escherichia coli
?
L-1-aminopropan-2-ol + NADP+
-
287299
Escherichia coli
aminoacetone + NADPH
-
287299
Escherichia coli
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
cell free extract, second peak at pH 7.0-7.2
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6
10
two peaks at pH 7 and 10 with very low activity at pH 8.5, at pH 7 50% of maximum activity
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
required
Escherichia coli
NADP+
poor substitute for NAD+
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
required
Escherichia coli
NADP+
poor substitute for NAD+
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-chloromercuribenzoate
complete inhibition at 0.05 mM
Escherichia coli
8-hydroxyquinoline
nearly complete inhibition at 2.5 mM
Escherichia coli
D,L-1,3-diaminopropan-2-ol
-
Escherichia coli
D,L-2hydroxy-2-phenylethylamine
-
Escherichia coli
D,L-serine
-
Escherichia coli
D-1-amininopropan-2-ol
competitive
Escherichia coli
iodoacetate
more inhibitory in intact cells than in cell free extracts
Escherichia coli
L-1-(3,4-dihydroxyphenyl)-2-aminoethanol
noradrenalin
Escherichia coli
sodium carbonate/dicarbonate buffers
at alkaline pH
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
NAD+
at pH 7 with aminopropanol as substrate
Escherichia coli
0.4
-
NAD+
at pH 9.6 with aminopropanol as substrate
Escherichia coli
1.5
-
L-1-Aminopropan-2-ol
cell free extracts
Escherichia coli
10
-
D,L-1-aminopropan-2-ol
cell free extracts
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-1-aminopropan-2-ol + NAD+
Escherichia coli
involved in L-threonine and vitamin B12 pathway
aminoacetone + NADH
-
Escherichia coli
?
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
0.008
-
cell free extract
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
-15C stable for several days
Escherichia coli
0-5C significant loss in activity
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D,L-1-aminopropan-2-ol + NAD+
more active with L-enantiomer, activity with D-enantiomer might be due to D-aminopropanol specific enzyme
287299
Escherichia coli
aminoacetone + NADH
-
287299
Escherichia coli
?
D,L-aminobutan-2-ol + NAD+
-
287299
Escherichia coli
1-amino-2-oxobutane + NADH
-
-
-
?
D,L-phenylserine + NAD+
-
287299
Escherichia coli
?
-
-
-
?
L-1-aminopropan-2-ol + NAD+
-
287299
Escherichia coli
aminoacetone + NADH
-
287299
Escherichia coli
?
L-1-aminopropan-2-ol + NAD+
involved in L-threonine and vitamin B12 pathway
287299
Escherichia coli
aminoacetone + NADH
-
287299
Escherichia coli
?
L-1-aminopropan-2-ol + NADP+
-
287299
Escherichia coli
aminoacetone + NADPH
-
287299
Escherichia coli
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
cell free extract, second peak at pH 7.0-7.2
Escherichia coli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6
10
two peaks at pH 7 and 10 with very low activity at pH 8.5, at pH 7 50% of maximum activity
Escherichia coli
Other publictions for EC 1.1.1.75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
695805
Kataoka
Cloning and expression of the ...
Rhodococcus erythropolis, Rhodococcus erythropolis MAK154
Appl. Microbiol. Biotechnol.
80
597-604
2008
-
-
1
-
-
-
-
-
-
-
-
4
-
4
-
-
-
-
-
-
-
-
11
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
11
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
287303
Faulkner
Microbial metabolism of amino ...
Pseudomonas sp.
Biochem. J.
138
263-276
1974
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287302
Willetts
Threonine metabolism in a stra ...
Bacillus subtilis
Biochim. Biophys. Acta
252
98-104
1971
-
-
-
-
-
-
-
2
-
1
1
1
-
1
-
-
1
-
-
-
1
2
9
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
1
1
1
-
-
-
1
-
-
1
2
9
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
287300
Cox
Aminopropanol dehydrogenase ac ...
Rattus norvegicus
Biochim. Biophys. Acta
170
438-439
1968
-
-
-
-
-
-
3
3
1
-
-
1
-
1
-
-
-
-
-
1
1
1
3
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
3
1
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
287304
Pickard
Purification and properties of ...
Pseudomonas sp.
J. Gen. Microbiol.
54
115-126
1968
-
-
-
-
-
1
1
3
-
-
1
1
-
1
-
-
1
-
-
-
3
1
9
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
3
-
-
1
1
-
-
-
1
-
-
3
1
9
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287299
Turner
Microbial metabolism of amino ...
Escherichia coli
Biochem. J.
104
112-121
1967
-
-
-
-
-
-
9
4
-
-
-
1
-
1
-
-
1
-
-
-
1
2
6
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
9
-
4
-
-
-
1
-
-
-
1
-
-
1
2
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287301
Turner
Amino ketone formation and ami ...
Rattus norvegicus
Biochem. J.
102
511-519
1967
-
-
-
-
-
1
4
3
1
2
-
1
-
1
-
-
-
-
-
5
1
-
4
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
3
1
2
-
1
-
-
-
-
-
5
1
-
4
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287298
Turner
Microbial metabolism of amino ...
Escherichia coli
Biochem. J.
99
427-433
1966
-
-
-
-
-
-
2
2
-
3
-
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
3
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287305
Pickard
-
Microbial metabolism of 1-amin ...
Pseudomonas sp.
J. Gen. Microbiol.
45
i-ii
1966
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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3
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1
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3
-
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-
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-
-
-
-
-
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-
-
-
-
287306
Higgins
-
Microbial metabolism of aminoa ...
Pseudomonas sp.
Biochem. J.
99
26P-27P
1966
-
-
-
-
-
-
1
-
1
-
-
1
-
1
-
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-
-
-
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1
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1
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1
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1
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1
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-
-
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-
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-
-
-
287307
Higgins
-
1-Aminopropan-2-ol-NAD oxidore ...
Escherichia coli, Rattus norvegicus
Biochem. J.
99
27P
1966
-
-
-
-
-
-
-
-
1
2
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2
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2
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1
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5
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3
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1
2
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2
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1
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5
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3
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