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show all sequences of 1.1.1.75

Microbial metabolism of amino ketones. Aminoacetone formation from 1-aminopropan-2-ol by a dehydrgenase in Escerichia coli

Turner, J.M.; Biochem. J. 99, 427-433 (1966)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
D,L-2hydroxy-2-phenylethylamine
slight inhibition
Escherichia coli
D,L-3-hydroxybutyrate
slight inhibition
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
1-aminopropan-2-ol
washed cell suspension
Escherichia coli
0.8
-
1-aminopropan-2-ol
cell free extracts
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu2+
slight inhibition
Escherichia coli
Fe2+
slightly activating
Escherichia coli
K+
required
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-1-aminopropan-2-ol + NAD+
Escherichia coli
-
aminoacetone + NADH
-
Escherichia coli
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.003
-
under optimum conditions
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D,L-1-aminopropan-2-ol + NAD+
more active with L-enantiomer, activity with D-enantiomer might be due to D-aminopropanol specific enzyme
287298
Escherichia coli
aminoacetone + NADH
-
287298
Escherichia coli
?
L-1-aminopropan-2-ol + NAD+
-
287298
Escherichia coli
aminoacetone + NADH
-
287298
Escherichia coli
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4
-
washed cell suspension
Escherichia coli
7
-
cell free extracts
Escherichia coli
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.5
8
washed cell suspension, very low activity at 4.5, 50% activity at pH 8
Escherichia coli
6.5
9
cell free extracts, 50% activity at both pH
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D,L-2hydroxy-2-phenylethylamine
slight inhibition
Escherichia coli
D,L-3-hydroxybutyrate
slight inhibition
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.3
-
1-aminopropan-2-ol
washed cell suspension
Escherichia coli
0.8
-
1-aminopropan-2-ol
cell free extracts
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu2+
slight inhibition
Escherichia coli
Fe2+
slightly activating
Escherichia coli
K+
required
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-1-aminopropan-2-ol + NAD+
Escherichia coli
-
aminoacetone + NADH
-
Escherichia coli
?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.003
-
under optimum conditions
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D,L-1-aminopropan-2-ol + NAD+
more active with L-enantiomer, activity with D-enantiomer might be due to D-aminopropanol specific enzyme
287298
Escherichia coli
aminoacetone + NADH
-
287298
Escherichia coli
?
L-1-aminopropan-2-ol + NAD+
-
287298
Escherichia coli
aminoacetone + NADH
-
287298
Escherichia coli
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.4
-
washed cell suspension
Escherichia coli
7
-
cell free extracts
Escherichia coli
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.5
8
washed cell suspension, very low activity at 4.5, 50% activity at pH 8
Escherichia coli
6.5
9
cell free extracts, 50% activity at both pH
Escherichia coli
Other publictions for EC 1.1.1.75
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
695805
Kataoka
Cloning and expression of the ...
Rhodococcus erythropolis, Rhodococcus erythropolis MAK154
Appl. Microbiol. Biotechnol.
80
597-604
2008
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-
1
-
-
-
-
-
-
-
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4
-
4
-
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-
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-
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11
-
1
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1
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1
-
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1
1
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4
-
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-
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-
-
-
11
-
1
-
-
-
1
-
-
-
-
-
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-
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-
287303
Faulkner
Microbial metabolism of amino ...
Pseudomonas sp.
Biochem. J.
138
263-276
1974
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
2
-
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-
-
1
-
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-
-
-
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-
1
-
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-
-
-
1
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287302
Willetts
Threonine metabolism in a stra ...
Bacillus subtilis
Biochim. Biophys. Acta
252
98-104
1971
-
-
-
-
-
-
-
2
-
1
1
1
-
1
-
-
1
-
-
-
1
2
9
-
1
1
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1
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-
-
-
-
2
-
1
1
1
-
-
-
1
-
-
1
2
9
-
1
1
-
-
1
-
-
-
-
-
-
-
-
-
287300
Cox
Aminopropanol dehydrogenase ac ...
Rattus norvegicus
Biochim. Biophys. Acta
170
438-439
1968
-
-
-
-
-
-
3
3
1
-
-
1
-
1
-
-
-
-
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1
1
1
3
-
-
-
2
-
1
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-
-
-
-
-
-
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3
-
3
1
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1
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1
1
1
3
-
-
-
2
-
1
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-
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-
287304
Pickard
Purification and properties of ...
Pseudomonas sp.
J. Gen. Microbiol.
54
115-126
1968
-
-
-
-
-
1
1
3
-
-
1
1
-
1
-
-
1
-
-
-
3
1
9
-
-
-
-
-
1
1
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-
-
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-
-
-
-
-
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-
1
-
1
-
3
-
-
1
1
-
-
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1
-
-
3
1
9
-
-
-
-
-
1
1
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-
-
-
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-
-
-
287299
Turner
Microbial metabolism of amino ...
Escherichia coli
Biochem. J.
104
112-121
1967
-
-
-
-
-
-
9
4
-
-
-
1
-
1
-
-
1
-
-
-
1
2
6
-
-
-
-
-
1
1
-
2
-
-
-
-
-
-
2
-
-
-
-
9
-
4
-
-
-
1
-
-
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1
-
-
1
2
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
287301
Turner
Amino ketone formation and ami ...
Rattus norvegicus
Biochem. J.
102
511-519
1967
-
-
-
-
-
1
4
3
1
2
-
1
-
1
-
-
-
-
-
5
1
-
4
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
3
1
2
-
1
-
-
-
-
-
5
1
-
4
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287298
Turner
Microbial metabolism of amino ...
Escherichia coli
Biochem. J.
99
427-433
1966
-
-
-
-
-
-
2
2
-
3
-
1
-
1
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1
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2
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2
2
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2
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2
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3
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1
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1
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2
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2
2
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287305
Pickard
-
Microbial metabolism of 1-amin ...
Pseudomonas sp.
J. Gen. Microbiol.
45
i-ii
1966
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1
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1
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1
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3
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287306
Higgins
-
Microbial metabolism of aminoa ...
Pseudomonas sp.
Biochem. J.
99
26P-27P
1966
-
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1
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1
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1
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1
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1
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1
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1
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287307
Higgins
-
1-Aminopropan-2-ol-NAD oxidore ...
Escherichia coli, Rattus norvegicus
Biochem. J.
99
27P
1966
-
-
-
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1
2
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2
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2
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1
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5
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3
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1
2
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2
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1
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5
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3
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