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show all sequences of 1.1.1.72

The Three-Dimensional Structure of AKR11B4, a Glycerol Dehydrogenase from Gluconobacter oxydans, Reveals a Tryptophan Residue as an Accelerator of Reaction Turnover

Richter, N.; Breicha, K.; Hummel, W.; Niefind, K.; J. Mol. Biol. 404, 353-362 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
His-tagged version
Gluconobacter oxydans
Crystallization (Commentary)
Crystallization
Organism
sitting-drop variant of the vapor diffusion method
Gluconobacter oxydans
Engineering
Amino acid exchange
Commentary
Organism
W23A
W23 is a key residue (accelerator) for NADP/NADPH turnover
Gluconobacter oxydans
W23M
W23 is a key residue (accelerator) for NADP/NADPH turnover
Gluconobacter oxydans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
KM-value 118.0 mM with substrate DL-glyceraldehyde, W23A mutant protein, pH 7.0, 30°C; KM-value 2.4 mM with substrate DL-glyceraldehyde, wild-type protein, pH 7.0, 30°C; KM-value 49.4 mM with substrate DL-glyceraldehyde, W23M mutant protein, pH 7.0, 30°C
Gluconobacter oxydans
0.0041
-
NADPH
wild-type protein, pH 7.0, 30°C
Gluconobacter oxydans
0.0062
-
NADPH
W23A mutant protein, pH 7.0, 30°C; W23M mutant protein, pH 7.0, 30°C
Gluconobacter oxydans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37213
-
37213, calculated
Gluconobacter oxydans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter oxydans
Q5FQJ0
-
-
Purification (Commentary)
Commentary
Organism
-
Gluconobacter oxydans
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2
-
W23A mutant protein, NADPH as variable substrate, pH 7.0, 30°C
Gluconobacter oxydans
4.9
-
W23A mutant protein, DL-glyceraldehyde as variable substrate, pH 7.0, 30°C
Gluconobacter oxydans
37
-
W23M mutant protein, DL-glyceraldehyde as variable substrate, pH 7.0, 30°C
Gluconobacter oxydans
54
-
W23M mutant protein, NADPH as variable substrate, pH 7.0, 30°C
Gluconobacter oxydans
80.5
-
wild-type protein, DL-glyceraldehyde as variable substrate, pH 7.0, 30°C
Gluconobacter oxydans
87.1
-
wild-type protein, NADPH as variable substrate, pH 7.0, 30°C
Gluconobacter oxydans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
DL-glyceraldehyde + NADPH + H+
significant enantioselectivity
712771
Gluconobacter oxydans
glycerol + NADP+
-
-
-
r
glycerol + NADP+
-
712771
Gluconobacter oxydans
D-glyceraldehyde + NADPH + H+
significant enantioselectivity
-
-
r
Subunits
Subunits
Commentary
Organism
monomer
37213, calculated
Gluconobacter oxydans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
24.2/sec with substrate D/L-glyceraldehyde, W23M mutant protein, pH 7.0, 30°C; 3.2/sec with substrate D/L-glyceraldehyde, W23A mutant protein, pH 7.0, 30°C; 52.8/sec with substrate D/L-glyceraldehyde, wild-type protein, pH 7.0, 30°C
Gluconobacter oxydans
2.7
-
NADPH
W23A mutant protein, pH 7.0, 30°C
Gluconobacter oxydans
35.4
-
NADPH
W23M mutant protein, pH 7.0, 30°C
Gluconobacter oxydans
57.2
-
NADPH
wild-type protein, pH 7.0, 30°C
Gluconobacter oxydans
Cloned(Commentary) (protein specific)
Commentary
Organism
His-tagged version
Gluconobacter oxydans
Crystallization (Commentary) (protein specific)
Crystallization
Organism
sitting-drop variant of the vapor diffusion method
Gluconobacter oxydans
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
W23A
W23 is a key residue (accelerator) for NADP/NADPH turnover
Gluconobacter oxydans
W23M
W23 is a key residue (accelerator) for NADP/NADPH turnover
Gluconobacter oxydans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
KM-value 118.0 mM with substrate DL-glyceraldehyde, W23A mutant protein, pH 7.0, 30°C; KM-value 2.4 mM with substrate DL-glyceraldehyde, wild-type protein, pH 7.0, 30°C; KM-value 49.4 mM with substrate DL-glyceraldehyde, W23M mutant protein, pH 7.0, 30°C
Gluconobacter oxydans
0.0041
-
NADPH
wild-type protein, pH 7.0, 30°C
Gluconobacter oxydans
0.0062
-
NADPH
W23A mutant protein, pH 7.0, 30°C; W23M mutant protein, pH 7.0, 30°C
Gluconobacter oxydans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37213
-
37213, calculated
Gluconobacter oxydans
Purification (Commentary) (protein specific)
Commentary
Organism
-
Gluconobacter oxydans
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.2
-
W23A mutant protein, NADPH as variable substrate, pH 7.0, 30°C
Gluconobacter oxydans
4.9
-
W23A mutant protein, DL-glyceraldehyde as variable substrate, pH 7.0, 30°C
Gluconobacter oxydans
37
-
W23M mutant protein, DL-glyceraldehyde as variable substrate, pH 7.0, 30°C
Gluconobacter oxydans
54
-
W23M mutant protein, NADPH as variable substrate, pH 7.0, 30°C
Gluconobacter oxydans
80.5
-
wild-type protein, DL-glyceraldehyde as variable substrate, pH 7.0, 30°C
Gluconobacter oxydans
87.1
-
wild-type protein, NADPH as variable substrate, pH 7.0, 30°C
Gluconobacter oxydans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
DL-glyceraldehyde + NADPH + H+
significant enantioselectivity
712771
Gluconobacter oxydans
glycerol + NADP+
-
-
-
r
glycerol + NADP+
-
712771
Gluconobacter oxydans
D-glyceraldehyde + NADPH + H+
significant enantioselectivity
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
37213, calculated
Gluconobacter oxydans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
24.2/sec with substrate D/L-glyceraldehyde, W23M mutant protein, pH 7.0, 30°C; 3.2/sec with substrate D/L-glyceraldehyde, W23A mutant protein, pH 7.0, 30°C; 52.8/sec with substrate D/L-glyceraldehyde, wild-type protein, pH 7.0, 30°C
Gluconobacter oxydans
2.7
-
NADPH
W23A mutant protein, pH 7.0, 30°C
Gluconobacter oxydans
35.4
-
NADPH
W23M mutant protein, pH 7.0, 30°C
Gluconobacter oxydans
57.2
-
NADPH
wild-type protein, pH 7.0, 30°C
Gluconobacter oxydans
Other publictions for EC 1.1.1.72
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711534
Richter
Characterization of a whole-ce ...
Gluconobacter oxydans
Biotechnol. Bioeng.
106
541-552
2010
-
-
1
-
-
-
-
-
-
-
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4
-
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1
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4
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1
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1
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4
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
712771
Richter
The Three-Dimensional Structur ...
Gluconobacter oxydans
J. Mol. Biol.
404
353-362
2010
-
-
1
1
2
-
-
3
-
-
1
-
-
2
-
-
1
-
-
-
6
-
2
1
-
-
-
4
-
-
-
-
-
-
-
-
-
1
-
1
2
-
-
-
-
3
-
-
1
-
-
-
-
1
-
-
6
-
2
1
-
-
-
4
-
-
-
-
-
-
-
-
-
-
711743
Richter
Characterisation of a recombin ...
Gluconobacter oxydans
ChemBioChem
10
1888-1896
2009
-
-
1
-
-
-
1
4
-
-
2
-
-
4
-
-
1
-
-
-
-
-
45
1
3
-
2
-
4
-
1
2
1
-
-
-
-
1
2
-
-
-
-
1
1
4
-
-
2
-
-
-
-
1
-
-
-
-
45
1
3
-
2
-
4
-
1
-
-
-
-
-
-
-
656732
Ruijter
Polyol accumulation by Aspergi ...
Aspergillus oryzae
Microbiology
150
1095-1101
2004
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
-
-
1
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656831
de Vries
Glycerol dehydrogenase, encode ...
Aspergillus nidulans
Mol. Microbiol.
49
131-141
2003
1
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
1
-
1
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
287291
Sealy-Lewis H.M.; Fairhurst V.
An NADP+-dependent glycerol de ...
Aspergillus nidulans
Curr. Genet.
22
293-296
1992
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
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-
-
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-
1
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-
-
-
-
-
-
-
-
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-
-
-
-
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-
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-
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-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287284
Castro
Glycerol utilization in Fusari ...
Fusarium oxysporum
J. Gen. Microbiol.
137
1497-1502
1991
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
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2
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2
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2
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-
1
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-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287289
Schuurink
Purification and properties of ...
Aspergillus nidulans, Aspergillus niger
J. Gen. Microbiol.
136
1043-1050
1990
-
-
-
-
-
1
-
-
-
-
2
-
-
4
-
-
2
-
-
2
2
-
10
2
-
-
-
-
4
-
-
4
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
2
-
-
-
-
2
-
2
2
-
10
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
287290
Inayat
-
Regulation of NADP+-specific g ...
Aspergillus niger
Biochem. Soc. Trans.
16
976-977
1988
-
-
-
-
-
-
2
-
-
1
-
-
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1
-
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1
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1
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2
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2
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2
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1
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1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287283
Flynn
Glycerol dehydrogenase from ra ...
Oryctolagus cuniculus
Methods Enzymol.
89
237-242
1982
-
-
-
-
-
-
4
1
-
-
1
1
-
1
-
-
1
-
-
1
1
1
13
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
4
-
1
-
-
1
1
-
-
-
1
-
1
1
1
13
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287285
Viswanath-Reddy
-
Purification and properties of ...
Neurospora crassa
J. Gen. Microbiol.
107
289-296
1978
-
-
-
-
-
-
1
4
-
-
2
1
-
1
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1
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1
1
5
1
-
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2
-
2
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2
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2
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1
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4
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2
1
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1
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1
1
5
1
-
-
2
-
2
-
-
-
-
-
-
-
-
-
287288
Walton
Stereochemistry of reduction o ...
Oryctolagus cuniculus
Biochemistry
12
3472-3478
1973
-
-
-
-
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1
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1
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1
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1
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1
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2
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-
-
-
-
287286
Kormann
Purification and properties of ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
258
40-55
1972
-
-
-
-
-
-
2
2
-
-
1
1
-
1
-
-
1
-
-
1
-
1
9
-
-
-
-
-
1
-
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3
-
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3
-
-
-
-
2
-
2
-
-
1
1
-
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1
-
1
-
1
9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287287
Toews
The kinetics and reaction mech ...
Rattus norvegicus
Biochem. J.
105
1067-1073
1967
-
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1
4
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1
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1
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1
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1
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6
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2
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2
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1
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1
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1
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1
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6
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