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show all sequences of 1.1.1.72

Characterisation of a recombinant NADP-dependent glycerol dehydrogenase from Gluconobacter oxydans and its application in the production of L-glyceraldehyde

Richter, N.; Neumann, M.; Liese, A.; Wohlgemuth, R.; Eggert, T.; Hummel, W.; ChemBioChem 10, 1888-1896 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpressed in Escherichia coli as a His-tagged fusion protein
Gluconobacter oxydans
Inhibitors
Inhibitors
Commentary
Organism
Structure
NADPH
inhibition if concentration is above 0.15 mM
Gluconobacter oxydans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NADP+
oxidation, pH 9.7, 37°C, Vmax: 2.1 micromol/min/mg
Gluconobacter oxydans
0.04
-
NADPH
reduction, pH 7.0, 30°C, Vmax: 101 micromol/min/mg
Gluconobacter oxydans
2.69
-
D-glyceraldehyde
reduction, pH 7.0, 30°C, Vmax: 77.9 micromol/min/mg
Gluconobacter oxydans
193
-
L-arabitol
oxidation, pH 9.7, 37°C, Vmax: 8.67 micromol/min/mg
Gluconobacter oxydans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37210
-
calculated from cDNA
Gluconobacter oxydans
39000
-
SDS-PAGE
Gluconobacter oxydans
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Gluconobacter oxydans
-
-
-
Purification (Commentary)
Commentary
Organism
using Ni-NTA chromatography
Gluconobacter oxydans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2,2-dimethyl-1,3-dioxolan-4-yl)methanol + NADP+
oxidation reaction. Specific activity: 0.03 micromol/min/mg
711743
Gluconobacter oxydans
2,2-dimethyl-1,3-dioxolane-4-carbaldehyde + NADPH
-
-
-
?
(2E)-pent-2-enal + NADPH + H+
reduction reaction: specific activity: 4.9 micromol/min/mg. Oxidation reaction specific activity: 0.74 micromol/min/mg
711743
Gluconobacter oxydans
(2E)-pent-2-enol + NADP+
-
-
-
?
(2R)-2,4-dihydroxybutanal + NADPH + H+
reduction reaction. Specific activity: 46.6 micromol/min/mg
711743
Gluconobacter oxydans
(2R)-2,4-dihydroxybutanol + NADP+
-
-
-
?
(2R,3S)-butane-1,2,3,4-tetrol + NADP+
oxidation reaction. Specific activity: 0.75 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
(2R,3S,4R,5S)-hexane-1,2,3,4,5,6-hexol + NADP+
oxidation reaction. Specific activity: 1.08 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
(2R,3S,4S)-pentane-1,2,3,4,5-pentol + NADP+
oxidation reaction. Specific activity: 0.25 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
(2R,4S)-pentane-1,2,3,4,5-pentol + NADP+
oxidation reaction. Specific activity: 2.46 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
(2S)-2,4-dihydroxybutanal + NADPH + H+
reduction reaction. Specific activity: 2.0 micromol/min/mg
711743
Gluconobacter oxydans
(2S)-2,4-dihydroxybutanol + NADP+
-
-
-
?
(2S,3S)-butane-1,2,3,4-tetrol + NADP+
oxidation reaction. Specific activity: 0.9 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
1,3-dihydroxypropan-2-one + NADPH + H+
reduction reaction. Specific activity: 0.5 micromol/min/mg
711743
Gluconobacter oxydans
propane-1,2,3-triol + NADP+
-
-
-
?
1-phenylethane-1,2-diol + NADP+
oxidation reaction. Specific activity: 0.77 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
1-phenylethanone + NADPH + H+
reduction reaction. Specific activity: 0.04 micromol/min/mg
711743
Gluconobacter oxydans
1-phenylethanol + NADP+
-
-
-
?
1-phenylpropanone + NADPH + H+
reduction reaction. Specific activity: 0.03 micromol/min/mg
711743
Gluconobacter oxydans
1-phenylpropanol + NADP+
-
-
-
?
2,4-difluorobenzaldehyde + NADPH + H+
reduction reaction. Specific activity: 19.6 micromol/min/mg
711743
Gluconobacter oxydans
(2,4-difluorophenyl)methanol + NADP+
-
-
-
?
2,4-dihydroxybutanal + NADPH + H+
reduction reaction. Specific activity: 39.1 micromol/min/mg
711743
Gluconobacter oxydans
2,4-dihydroxybutanol + NADP+
-
-
-
?
2-methylbutanal + NADPH + H+
reduction reaction. Specific activity: 26.8 micromol/min/mg
711743
Gluconobacter oxydans
2-methylbutanol + NADP+
-
-
-
?
2-methylpentanal + NADPH + H+
reduction reaction. Specific activity: 25.7 micromol/min/mg
711743
Gluconobacter oxydans
2-methylpentanol + NADP+
-
-
-
?
2-methylpropanal + NADPH + H+
reduction reaction. Specific activity: 6.7 micromol/min/mg
711743
Gluconobacter oxydans
2-methylpropanol + NADP+
-
-
-
?
4-methoxybenzaldehyde + NADPH + H+
reduction reaction. Specific activity: 0.3 micromol/min/mg
711743
Gluconobacter oxydans
(4-methoxyphenyl)methanol + NADP+
-
-
-
?
6-methylheptane-2,5-dione + NADPH + H+
reduction reaction. Specific activity: 0.03 micromol/min/mg
711743
Gluconobacter oxydans
? + NADP+
-
-
-
?
acetaldehyde + NADPH + H+
reduction reaction. Specific activity: 0.1 micromol/min/mg
711743
Gluconobacter oxydans
ethanol + NADP+
-
-
-
?
benzaldehyde + NADPH + H+
reduction reaction. Specific activity: 13.8 micromol/min/mg
711743
Gluconobacter oxydans
benzyl alcohol + NADP+
-
-
-
?
butanal + NADPH + H+
reduction reaction. Specific activity: 23.3 micromol/min/mg
711743
Gluconobacter oxydans
butanol + NADP+
-
-
-
?
butane-1,2,4-triol + NADP+
oxidation reaction. Specific activity: 1.21 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
butane-1,2-diol + NADP+
oxidation reaction. Specific activity: 0.66 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
butane-1,3-diol + NADP+
oxidation reaction. Specific activity: 0.04 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
butane-1,4-diol + NADP+
oxidation reaction. Specific activity: 0.21 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
D-glyceraldehyde + NADPH + H+
enzyme favors reduction reaction
711743
Gluconobacter oxydans
glycerol + NADP+
-
-
-
?
dodecanal + NADPH + H+
reduction reaction. Specific activity: 19.3 micromol/min/mg
711743
Gluconobacter oxydans
dodecanol + NADP+
-
-
-
?
ethane-1,1-diol + NADP+
oxidation reaction. Specific activity: 0.5 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
glycerol + NADP+
oxidation reaction. Specific activity: 0.17 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
hexan-2-ol + NADP+
oxidation reaction. Specific activity: 0.22 micromol/min/mg
711743
Gluconobacter oxydans
hexan-2-one + NADPH
-
-
-
?
hexanal + NADPH + H+
reduction reaction. Specific activity: 31.9 micromol/min/mg
711743
Gluconobacter oxydans
hexanol + NADP+
-
-
-
r
hexane-1,2,3-triol + NADP+
oxidation reaction. Specific activity: 0.76 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
hexane-1,2,6-triol + NADP+
oxidation reaction. Specific activity: 0.3 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
hexane-1,2,6-triol + NADP+
oxidation reaction. Specific activity: 0.45 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
hexane-1,6-diol + NADP+
oxidation reaction. Specific activity: 0.87 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
L-arabitol + NADP+
oxidation reaction
711743
Gluconobacter oxydans
? + NADPH
-
-
-
-
methanediol + NADP+
oxidation reaction. Specific activity: 0.05 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
nonanal + NADPH + H+
reduction reaction. Specific activity: 15.9 micromol/min/mg
711743
Gluconobacter oxydans
nonanol + NADP+
-
-
-
?
octan-3-one + NADPH + H+
reduction reaction. Specific activity: 0.02 micromol/min/mg
711743
Gluconobacter oxydans
octan-3-ol + NADP+
-
-
-
?
octanal + NADPH + H+
reduction reaction. Specific activity: 35.5 micromol/min/mg
711743
Gluconobacter oxydans
octanol + NADP+
-
-
-
?
pentane-1,2-diol + NADP+
oxidation reaction. Specific activity: 0.39 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
pentane-1,5-diol + NADP+
oxidation reaction. Specific activity: 0.59 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
propanal + NADPH + H+
reduction reaction. Specific activity: 1.1 micromol/min/mg
711743
Gluconobacter oxydans
propanol + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 39000, SDS-PAGE
Gluconobacter oxydans
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
reduction assay at
Gluconobacter oxydans
37
-
oxidation assay at
Gluconobacter oxydans
55
-
optimal activity
Gluconobacter oxydans
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4
30
stable between
Gluconobacter oxydans
50
-
rapid inactivation occurs
Gluconobacter oxydans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
optimal activity for reduction
Gluconobacter oxydans
7
-
reduction assay at
Gluconobacter oxydans
9.7
-
oxidation assay at
Gluconobacter oxydans
10
-
optimal pH for axidation
Gluconobacter oxydans
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
9.7
enzyme is stable and shows a residual activity of at least 82%
Gluconobacter oxydans
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Gluconobacter oxydans
NADPH
prefers NADPH over NADH. Using NADPH, a 200fold increase in activity compared to the activity achieved with the same concentration of NADH is observed
Gluconobacter oxydans
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.4
-
NADPH
pH 7.0, 30 °C
Gluconobacter oxydans
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpressed in Escherichia coli as a His-tagged fusion protein
Gluconobacter oxydans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Gluconobacter oxydans
NADPH
prefers NADPH over NADH. Using NADPH, a 200fold increase in activity compared to the activity achieved with the same concentration of NADH is observed
Gluconobacter oxydans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
NADPH
inhibition if concentration is above 0.15 mM
Gluconobacter oxydans
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.4
-
NADPH
pH 7.0, 30 °C
Gluconobacter oxydans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NADP+
oxidation, pH 9.7, 37°C, Vmax: 2.1 micromol/min/mg
Gluconobacter oxydans
0.04
-
NADPH
reduction, pH 7.0, 30°C, Vmax: 101 micromol/min/mg
Gluconobacter oxydans
2.69
-
D-glyceraldehyde
reduction, pH 7.0, 30°C, Vmax: 77.9 micromol/min/mg
Gluconobacter oxydans
193
-
L-arabitol
oxidation, pH 9.7, 37°C, Vmax: 8.67 micromol/min/mg
Gluconobacter oxydans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37210
-
calculated from cDNA
Gluconobacter oxydans
39000
-
SDS-PAGE
Gluconobacter oxydans
Purification (Commentary) (protein specific)
Commentary
Organism
using Ni-NTA chromatography
Gluconobacter oxydans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2,2-dimethyl-1,3-dioxolan-4-yl)methanol + NADP+
oxidation reaction. Specific activity: 0.03 micromol/min/mg
711743
Gluconobacter oxydans
2,2-dimethyl-1,3-dioxolane-4-carbaldehyde + NADPH
-
-
-
?
(2E)-pent-2-enal + NADPH + H+
reduction reaction: specific activity: 4.9 micromol/min/mg. Oxidation reaction specific activity: 0.74 micromol/min/mg
711743
Gluconobacter oxydans
(2E)-pent-2-enol + NADP+
-
-
-
?
(2R)-2,4-dihydroxybutanal + NADPH + H+
reduction reaction. Specific activity: 46.6 micromol/min/mg
711743
Gluconobacter oxydans
(2R)-2,4-dihydroxybutanol + NADP+
-
-
-
?
(2R,3S)-butane-1,2,3,4-tetrol + NADP+
oxidation reaction. Specific activity: 0.75 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
(2R,3S,4R,5S)-hexane-1,2,3,4,5,6-hexol + NADP+
oxidation reaction. Specific activity: 1.08 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
(2R,3S,4S)-pentane-1,2,3,4,5-pentol + NADP+
oxidation reaction. Specific activity: 0.25 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
(2R,4S)-pentane-1,2,3,4,5-pentol + NADP+
oxidation reaction. Specific activity: 2.46 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
(2S)-2,4-dihydroxybutanal + NADPH + H+
reduction reaction. Specific activity: 2.0 micromol/min/mg
711743
Gluconobacter oxydans
(2S)-2,4-dihydroxybutanol + NADP+
-
-
-
?
(2S,3S)-butane-1,2,3,4-tetrol + NADP+
oxidation reaction. Specific activity: 0.9 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
1,3-dihydroxypropan-2-one + NADPH + H+
reduction reaction. Specific activity: 0.5 micromol/min/mg
711743
Gluconobacter oxydans
propane-1,2,3-triol + NADP+
-
-
-
?
1-phenylethane-1,2-diol + NADP+
oxidation reaction. Specific activity: 0.77 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
1-phenylethanone + NADPH + H+
reduction reaction. Specific activity: 0.04 micromol/min/mg
711743
Gluconobacter oxydans
1-phenylethanol + NADP+
-
-
-
?
1-phenylpropanone + NADPH + H+
reduction reaction. Specific activity: 0.03 micromol/min/mg
711743
Gluconobacter oxydans
1-phenylpropanol + NADP+
-
-
-
?
2,4-difluorobenzaldehyde + NADPH + H+
reduction reaction. Specific activity: 19.6 micromol/min/mg
711743
Gluconobacter oxydans
(2,4-difluorophenyl)methanol + NADP+
-
-
-
?
2,4-dihydroxybutanal + NADPH + H+
reduction reaction. Specific activity: 39.1 micromol/min/mg
711743
Gluconobacter oxydans
2,4-dihydroxybutanol + NADP+
-
-
-
?
2-methylbutanal + NADPH + H+
reduction reaction. Specific activity: 26.8 micromol/min/mg
711743
Gluconobacter oxydans
2-methylbutanol + NADP+
-
-
-
?
2-methylpentanal + NADPH + H+
reduction reaction. Specific activity: 25.7 micromol/min/mg
711743
Gluconobacter oxydans
2-methylpentanol + NADP+
-
-
-
?
2-methylpropanal + NADPH + H+
reduction reaction. Specific activity: 6.7 micromol/min/mg
711743
Gluconobacter oxydans
2-methylpropanol + NADP+
-
-
-
?
4-methoxybenzaldehyde + NADPH + H+
reduction reaction. Specific activity: 0.3 micromol/min/mg
711743
Gluconobacter oxydans
(4-methoxyphenyl)methanol + NADP+
-
-
-
?
6-methylheptane-2,5-dione + NADPH + H+
reduction reaction. Specific activity: 0.03 micromol/min/mg
711743
Gluconobacter oxydans
? + NADP+
-
-
-
?
acetaldehyde + NADPH + H+
reduction reaction. Specific activity: 0.1 micromol/min/mg
711743
Gluconobacter oxydans
ethanol + NADP+
-
-
-
?
benzaldehyde + NADPH + H+
reduction reaction. Specific activity: 13.8 micromol/min/mg
711743
Gluconobacter oxydans
benzyl alcohol + NADP+
-
-
-
?
butanal + NADPH + H+
reduction reaction. Specific activity: 23.3 micromol/min/mg
711743
Gluconobacter oxydans
butanol + NADP+
-
-
-
?
butane-1,2,4-triol + NADP+
oxidation reaction. Specific activity: 1.21 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
butane-1,2-diol + NADP+
oxidation reaction. Specific activity: 0.66 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
butane-1,3-diol + NADP+
oxidation reaction. Specific activity: 0.04 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
butane-1,4-diol + NADP+
oxidation reaction. Specific activity: 0.21 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
D-glyceraldehyde + NADPH + H+
enzyme favors reduction reaction
711743
Gluconobacter oxydans
glycerol + NADP+
-
-
-
?
dodecanal + NADPH + H+
reduction reaction. Specific activity: 19.3 micromol/min/mg
711743
Gluconobacter oxydans
dodecanol + NADP+
-
-
-
?
ethane-1,1-diol + NADP+
oxidation reaction. Specific activity: 0.5 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
glycerol + NADP+
oxidation reaction. Specific activity: 0.17 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
hexan-2-ol + NADP+
oxidation reaction. Specific activity: 0.22 micromol/min/mg
711743
Gluconobacter oxydans
hexan-2-one + NADPH
-
-
-
?
hexanal + NADPH + H+
reduction reaction. Specific activity: 31.9 micromol/min/mg
711743
Gluconobacter oxydans
hexanol + NADP+
-
-
-
r
hexane-1,2,3-triol + NADP+
oxidation reaction. Specific activity: 0.76 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
hexane-1,2,6-triol + NADP+
oxidation reaction. Specific activity: 0.3 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
hexane-1,2,6-triol + NADP+
oxidation reaction. Specific activity: 0.45 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
hexane-1,6-diol + NADP+
oxidation reaction. Specific activity: 0.87 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
L-arabitol + NADP+
oxidation reaction
711743
Gluconobacter oxydans
? + NADPH
-
-
-
-
methanediol + NADP+
oxidation reaction. Specific activity: 0.05 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
nonanal + NADPH + H+
reduction reaction. Specific activity: 15.9 micromol/min/mg
711743
Gluconobacter oxydans
nonanol + NADP+
-
-
-
?
octan-3-one + NADPH + H+
reduction reaction. Specific activity: 0.02 micromol/min/mg
711743
Gluconobacter oxydans
octan-3-ol + NADP+
-
-
-
?
octanal + NADPH + H+
reduction reaction. Specific activity: 35.5 micromol/min/mg
711743
Gluconobacter oxydans
octanol + NADP+
-
-
-
?
pentane-1,2-diol + NADP+
oxidation reaction. Specific activity: 0.39 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
pentane-1,5-diol + NADP+
oxidation reaction. Specific activity: 0.59 micromol/min/mg
711743
Gluconobacter oxydans
? + NADPH
-
-
-
?
propanal + NADPH + H+
reduction reaction. Specific activity: 1.1 micromol/min/mg
711743
Gluconobacter oxydans
propanol + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 39000, SDS-PAGE
Gluconobacter oxydans
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
reduction assay at
Gluconobacter oxydans
37
-
oxidation assay at
Gluconobacter oxydans
55
-
optimal activity
Gluconobacter oxydans
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
4
30
stable between
Gluconobacter oxydans
50
-
rapid inactivation occurs
Gluconobacter oxydans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.5
-
optimal activity for reduction
Gluconobacter oxydans
7
-
reduction assay at
Gluconobacter oxydans
9.7
-
oxidation assay at
Gluconobacter oxydans
10
-
optimal pH for axidation
Gluconobacter oxydans
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
5.5
9.7
enzyme is stable and shows a residual activity of at least 82%
Gluconobacter oxydans
Other publictions for EC 1.1.1.72
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711534
Richter
Characterization of a whole-ce ...
Gluconobacter oxydans
Biotechnol. Bioeng.
106
541-552
2010
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1
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4
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1
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1
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4
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712771
Richter
The Three-Dimensional Structur ...
Gluconobacter oxydans
J. Mol. Biol.
404
353-362
2010
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-
1
1
2
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-
3
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1
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2
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1
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6
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2
1
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4
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1
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1
2
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3
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1
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1
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6
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2
1
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4
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711743
Richter
Characterisation of a recombin ...
Gluconobacter oxydans
ChemBioChem
10
1888-1896
2009
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1
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1
4
-
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2
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4
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1
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45
1
3
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2
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4
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1
2
1
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1
2
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1
1
4
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2
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1
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-
-
45
1
3
-
2
-
4
-
1
-
-
-
-
-
-
-
656732
Ruijter
Polyol accumulation by Aspergi ...
Aspergillus oryzae
Microbiology
150
1095-1101
2004
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-
-
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1
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1
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1
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1
4
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1
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1
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4
-
1
-
-
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-
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-
656831
de Vries
Glycerol dehydrogenase, encode ...
Aspergillus nidulans
Mol. Microbiol.
49
131-141
2003
1
-
1
-
-
-
-
-
-
-
1
1
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1
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-
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1
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2
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2
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1
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1
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1
2
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1
1
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-
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-
1
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2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
287291
Sealy-Lewis H.M.; Fairhurst V.
An NADP+-dependent glycerol de ...
Aspergillus nidulans
Curr. Genet.
22
293-296
1992
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1
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1
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-
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-
287284
Castro
Glycerol utilization in Fusari ...
Fusarium oxysporum
J. Gen. Microbiol.
137
1497-1502
1991
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-
-
-
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1
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1
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2
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1
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2
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-
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-
287289
Schuurink
Purification and properties of ...
Aspergillus nidulans, Aspergillus niger
J. Gen. Microbiol.
136
1043-1050
1990
-
-
-
-
-
1
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2
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4
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2
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2
2
-
10
2
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4
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4
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4
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1
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2
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2
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2
2
-
10
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
287290
Inayat
-
Regulation of NADP+-specific g ...
Aspergillus niger
Biochem. Soc. Trans.
16
976-977
1988
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2
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1
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1
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1
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1
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-
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1
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
287283
Flynn
Glycerol dehydrogenase from ra ...
Oryctolagus cuniculus
Methods Enzymol.
89
237-242
1982
-
-
-
-
-
-
4
1
-
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1
1
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1
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1
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1
1
1
13
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1
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3
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3
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4
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1
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1
1
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1
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1
1
1
13
-
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-
-
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1
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-
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-
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-
-
-
-
287285
Viswanath-Reddy
-
Purification and properties of ...
Neurospora crassa
J. Gen. Microbiol.
107
289-296
1978
-
-
-
-
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1
4
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2
1
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1
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1
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1
1
5
1
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2
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2
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2
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2
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1
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4
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2
1
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1
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1
1
5
1
-
-
2
-
2
-
-
-
-
-
-
-
-
-
287288
Walton
Stereochemistry of reduction o ...
Oryctolagus cuniculus
Biochemistry
12
3472-3478
1973
-
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2
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2
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1
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1
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2
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-
-
-
-
-
-
-
-
287286
Kormann
Purification and properties of ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
258
40-55
1972
-
-
-
-
-
-
2
2
-
-
1
1
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1
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1
-
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1
-
1
9
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
3
-
-
-
-
2
-
2
-
-
1
1
-
-
-
1
-
1
-
1
9
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
287287
Toews
The kinetics and reaction mech ...
Rattus norvegicus
Biochem. J.
105
1067-1073
1967
-
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1
4
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1
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1
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1
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1
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6
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-
-
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2
-
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-
2
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1
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4
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1
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1
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1
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6
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