BRENDA - Enzyme Database show
show all sequences of 1.1.1.72

Purification and properties of NADP(+)-dependent glycerol dehydrogenases from Aspergillus nidulans and A. niger

Schuurink, R.; Busink, R.; Hondmann, D.H.A.; Witteveen, C.F.B.; Visser, J.; J. Gen. Microbiol. 136, 1043-1050 (1990)

Data extracted from this reference:

General Stability
General Stability
Organism
partially inactivated by freezing and thawing
Aspergillus nidulans
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
SDS-PAGE
Aspergillus nidulans
38000
-
SDS-PAGE
Aspergillus niger
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Aspergillus nidulans
-
-
-
Aspergillus niger
-
-
-
Purification (Commentary)
Commentary
Organism
-
Aspergillus nidulans
-
Aspergillus niger
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
24.1
-
-
Aspergillus nidulans
32.5
-
-
Aspergillus niger
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-butanedione + NADPH
-
287289
Aspergillus nidulans
?
-
-
-
?
2,3-butanedione + NADPH
-
287289
Aspergillus niger
?
-
-
-
?
diacetyl + NADPH
-
287289
Aspergillus nidulans
?
-
-
-
?
diacetyl + NADPH
-
287289
Aspergillus niger
?
-
-
-
?
dihydroxyacetone + NADPH
-
287289
Aspergillus nidulans
glycerol + NADP+
-
-
-
r
dihydroxyacetone + NADPH
-
287289
Aspergillus niger
glycerol + NADP+
-
-
-
r
erythritol + NADP+
-
287289
Aspergillus nidulans
?
-
-
-
?
erythritol + NADP+
-
287289
Aspergillus niger
?
-
-
-
?
methylglyoxal + NADPH
-
287289
Aspergillus nidulans
1-hydroxyacetone + NADP+
-
-
-
?
methylglyoxal + NADPH
-
287289
Aspergillus niger
1-hydroxyacetone + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 38000, SDS-PAGE
Aspergillus nidulans
monomer
1 * 38000, SDS-PAGE
Aspergillus niger
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.8
-
D-glyceraldehyde reduction
Aspergillus nidulans
5.4
-
D-glyceraldehyde reduction
Aspergillus niger
10
-
glycerol oxidation
Aspergillus nidulans
10
-
glycerol oxidation
Aspergillus niger
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Aspergillus niger
NADP+
-
Aspergillus nidulans
NADPH
-
Aspergillus niger
NADPH
-
Aspergillus nidulans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Aspergillus nidulans
NADP+
-
Aspergillus niger
NADPH
-
Aspergillus nidulans
NADPH
-
Aspergillus niger
General Stability (protein specific)
General Stability
Organism
partially inactivated by freezing and thawing
Aspergillus nidulans
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
38000
-
SDS-PAGE
Aspergillus nidulans
38000
-
SDS-PAGE
Aspergillus niger
Purification (Commentary) (protein specific)
Commentary
Organism
-
Aspergillus nidulans
-
Aspergillus niger
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
24.1
-
-
Aspergillus nidulans
32.5
-
-
Aspergillus niger
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-butanedione + NADPH
-
287289
Aspergillus nidulans
?
-
-
-
?
2,3-butanedione + NADPH
-
287289
Aspergillus niger
?
-
-
-
?
diacetyl + NADPH
-
287289
Aspergillus nidulans
?
-
-
-
?
diacetyl + NADPH
-
287289
Aspergillus niger
?
-
-
-
?
dihydroxyacetone + NADPH
-
287289
Aspergillus nidulans
glycerol + NADP+
-
-
-
r
dihydroxyacetone + NADPH
-
287289
Aspergillus niger
glycerol + NADP+
-
-
-
r
erythritol + NADP+
-
287289
Aspergillus nidulans
?
-
-
-
?
erythritol + NADP+
-
287289
Aspergillus niger
?
-
-
-
?
methylglyoxal + NADPH
-
287289
Aspergillus nidulans
1-hydroxyacetone + NADP+
-
-
-
?
methylglyoxal + NADPH
-
287289
Aspergillus niger
1-hydroxyacetone + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 38000, SDS-PAGE
Aspergillus nidulans
monomer
1 * 38000, SDS-PAGE
Aspergillus niger
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.8
-
D-glyceraldehyde reduction
Aspergillus nidulans
5.4
-
D-glyceraldehyde reduction
Aspergillus niger
10
-
glycerol oxidation
Aspergillus nidulans
10
-
glycerol oxidation
Aspergillus niger
Other publictions for EC 1.1.1.72
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
711534
Richter
Characterization of a whole-ce ...
Gluconobacter oxydans
Biotechnol. Bioeng.
106
541-552
2010
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-
1
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-
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-
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4
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1
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4
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1
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1
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4
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712771
Richter
The Three-Dimensional Structur ...
Gluconobacter oxydans
J. Mol. Biol.
404
353-362
2010
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-
1
1
2
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3
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1
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2
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1
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6
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2
1
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4
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1
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1
2
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3
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1
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1
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6
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2
1
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4
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711743
Richter
Characterisation of a recombin ...
Gluconobacter oxydans
ChemBioChem
10
1888-1896
2009
-
-
1
-
-
-
1
4
-
-
2
-
-
4
-
-
1
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-
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45
1
3
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2
-
4
-
1
2
1
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-
1
2
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-
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-
1
1
4
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2
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1
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45
1
3
-
2
-
4
-
1
-
-
-
-
-
-
-
656732
Ruijter
Polyol accumulation by Aspergi ...
Aspergillus oryzae
Microbiology
150
1095-1101
2004
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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1
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1
4
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1
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1
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1
-
1
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
656831
de Vries
Glycerol dehydrogenase, encode ...
Aspergillus nidulans
Mol. Microbiol.
49
131-141
2003
1
-
1
-
-
-
-
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
2
-
1
-
1
-
1
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
287291
Sealy-Lewis H.M.; Fairhurst V.
An NADP+-dependent glycerol de ...
Aspergillus nidulans
Curr. Genet.
22
293-296
1992
-
-
-
-
-
-
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1
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1
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1
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-
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-
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-
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-
287284
Castro
Glycerol utilization in Fusari ...
Fusarium oxysporum
J. Gen. Microbiol.
137
1497-1502
1991
-
-
-
-
-
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-
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1
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1
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2
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2
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1
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2
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-
287289
Schuurink
Purification and properties of ...
Aspergillus nidulans, Aspergillus niger
J. Gen. Microbiol.
136
1043-1050
1990
-
-
-
-
-
1
-
-
-
-
2
-
-
4
-
-
2
-
-
2
2
-
10
2
-
-
-
-
4
-
-
4
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
-
2
-
-
-
-
2
-
2
2
-
10
2
-
-
-
-
4
-
-
-
-
-
-
-
-
-
287290
Inayat
-
Regulation of NADP+-specific g ...
Aspergillus niger
Biochem. Soc. Trans.
16
976-977
1988
-
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-
-
2
-
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1
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1
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1
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1
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2
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2
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2
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1
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1
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1
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-
-
-
-
-
-
-
-
-
-
-
-
287283
Flynn
Glycerol dehydrogenase from ra ...
Oryctolagus cuniculus
Methods Enzymol.
89
237-242
1982
-
-
-
-
-
-
4
1
-
-
1
1
-
1
-
-
1
-
-
1
1
1
13
-
-
-
-
-
1
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3
-
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-
-
-
-
3
-
-
-
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4
-
1
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-
1
1
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1
-
1
1
1
13
-
-
-
-
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1
-
-
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-
-
-
-
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287285
Viswanath-Reddy
-
Purification and properties of ...
Neurospora crassa
J. Gen. Microbiol.
107
289-296
1978
-
-
-
-
-
-
1
4
-
-
2
1
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1
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1
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1
1
5
1
-
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2
-
2
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2
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2
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1
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4
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2
1
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1
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1
1
5
1
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2
-
2
-
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-
-
-
-
-
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-
287288
Walton
Stereochemistry of reduction o ...
Oryctolagus cuniculus
Biochemistry
12
3472-3478
1973
-
-
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1
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1
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1
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2
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287286
Kormann
Purification and properties of ...
Oryctolagus cuniculus
Biochim. Biophys. Acta
258
40-55
1972
-
-
-
-
-
-
2
2
-
-
1
1
-
1
-
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1
-
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1
-
1
9
-
-
-
-
-
1
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3
-
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3
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2
-
2
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1
1
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1
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1
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1
9
-
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-
-
1
-
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-
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287287
Toews
The kinetics and reaction mech ...
Rattus norvegicus
Biochem. J.
105
1067-1073
1967
-
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1
4
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1
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1
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1
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1
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6
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2
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2
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1
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1
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1
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1
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6
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