BRENDA - Enzyme Database show
show all sequences of 1.1.1.71

Heterologous overexpression, purification and characterisation of an alcohol dehydrogenase (ADH2) from Halobacterium sp. NRC-1

Liliensiek, A.K.; Cassidy, J.; Gucciardo, G.; Whitely, C.; Paradisi, F.; Mol. Biotechnol. 55, 143-149 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
a hexahistidine-tagged recombinant version of the enzyme is heterologously overexpressed in Haloferax volcanii
Halobacterium salinarum
expresssion in Haloferax volcanii
Halobacterium sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
NADPH
pH 6.0, 50C
Halobacterium salinarum
0.05
-
NADPH
pH 6.0, 50C
Halobacterium sp.
0.2
-
NADP+
pH 10.0, 50C
Halobacterium salinarum
0.2
-
NADP+
pH 6.0, 50C
Halobacterium sp.
3.4
-
acetaldehyde
pH 6.0, 50C
Halobacterium salinarum
3.4
-
acetaldehyde
pH 6.0, 50C
Halobacterium sp.
21.4
-
1-propanol
pH 10.0, 50C
Halobacterium salinarum
21.4
-
1-propanol
pH 6.0, 50C
Halobacterium sp.
107.9
-
ethanol
pH 10.0, 50C
Halobacterium salinarum
107.9
-
ethanol
pH 6.0, 50C
Halobacterium sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
KCl
a preference for KCl over NaCl is observed, with best activity at 4 M KCl
Halobacterium salinarum
KCl
maximum activity in presence of 4 M KCl
Halobacterium sp.
NaCl
the enzyme requires high salt concentrations for its activity, a preference for KCl over NaCl is observed
Halobacterium salinarum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37300
-
x * 37300, calculated and SDS-PAGE
Halobacterium sp.
Organic Solvent Stability
Organic Solvent
Commentary
Organism
acetonitrile
24 h at 4C, 13% loss of activity at 5% (v/v), 10% loss of activity at 10% (v/v), complete loss of activity at 30% (v/v)
Halobacterium salinarum
acetonitrile
10%, 90% residual activity
Halobacterium sp.
dimethyl sulfoxide
24 h at 4C, 11% loss of activity at 5% (v/v), 9% loss of activity at 10% (v/v), 59% loss of activity at 30% (v/v)
Halobacterium salinarum
DMSO
10%, 91% residual activity
Halobacterium sp.
Methanol
24 h at 4C, 8% loss of activity at 5% (v/v), 15% loss of activity at 10% (v/v), 94% loss of activity at 30% (v/v)
Halobacterium salinarum
Methanol
10%, 85% residual activity
Halobacterium sp.
tetrahydrofuran
24 h at 4C, 37% loss of activity at 5% (v/v), 57% loss of activity at 10% (v/v), complete loss of activity at 30% (v/v)
Halobacterium salinarum
tetrahydrofuran
10%, 43% residual activity
Halobacterium sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Halobacterium salinarum
Q9HMB6
-
-
Halobacterium sp.
Q9HMB6
-
-
Halobacterium sp. NRC-1
Q9HMB6
-
-
Purification (Commentary)
Commentary
Organism
purified in one step by immobilised Ni-affinity chromatography
Halobacterium salinarum
Storage Stability
Storage Stability
Organism
-20C, 30 days, 80% residual activity
Halobacterium sp.
-20C, 30 days, enzyme retains 80% of its original activity
Halobacterium salinarum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-butanol + NADP+
80% compared to activity with 1-propanol
728288
Halobacterium salinarum
butanal + NADPH + H+
-
-
-
?
1-butanol + NADP+
80% of the activtiy with 1-propanol
728288
Halobacterium sp.
butanal + NADPH + H+
-
-
-
?
1-butanol + NADP+
80% of the activtiy with 1-propanol
728288
Halobacterium sp. NRC-1
butanal + NADPH + H+
-
-
-
?
1-phenylethanol + NADP+
35% compared to activity with 1-propanol
728288
Halobacterium salinarum
acetophenone + NADPH + H+
-
-
-
?
1-phenylethanol + NADP+
35% of the activtiy with 1-propanol
728288
Halobacterium sp.
1-phenylethanone + NADPH + H+
-
-
-
?
1-propanol + NAD+
dual cofactor dependency, NAD+ shows 60% of the activity with NADP+
728288
Halobacterium salinarum
propanal + NADH + H+
-
-
-
?
1-propanol + NADP+
-
728288
Halobacterium sp.
propanal + NADPH + H+
-
-
-
?
1-propanol + NADP+
dual cofactor dependency, NAD+ shows 60% of the activity with NADP+
728288
Halobacterium salinarum
propanal + NADPH + H+
-
-
-
?
1-propanol + NADP+
-
728288
Halobacterium sp. NRC-1
propanal + NADPH + H+
-
-
-
?
2-butanol + NADP+
30% compared to activity with 1-propanol
728288
Halobacterium salinarum
butanone + NADPH + H+
-
-
-
?
2-butanol + NADP+
30% of the activtiy with 1-propanol
728288
Halobacterium sp.
butanone + NADPH + H+
-
-
-
?
2-propanol + NADP+
25% compared to activity with 1-propanol
728288
Halobacterium salinarum
acetone + NADPH + H+
-
-
-
?
acetaldehyde + NADPH + H+
-
728288
Halobacterium sp.
ethanol + NADP+
-
-
-
?
acetaldehyde + NADPH + H+
-
728288
Halobacterium sp. NRC-1
ethanol + NADP+
-
-
-
?
benzyl alcohol + NADP+
20% compared to activity with 1-propanol
728288
Halobacterium salinarum
benzaldehyde + NADPH + H+
-
-
-
?
ethanol + NADP+
85% compared to activity with 1-propanol
728288
Halobacterium salinarum
acetaldehyde + NADPH + H+
-
-
-
?
ethanol + NADP+
84% of the activtiy with 1-propanol
728288
Halobacterium sp.
acetaldehyde + NADPH + H+
-
-
-
?
ethanol + NADP+
84% of the activtiy with 1-propanol
728288
Halobacterium sp. NRC-1
acetaldehyde + NADPH + H+
-
-
-
?
additional information
preference for short-chain alcohol substrates, particularly for 1-propanol (100%) and ethanol (84%), no activity with glycerol or methanol
728288
Halobacterium salinarum
?
-
-
-
-
additional information
no substrates: methanol, glycerol
728288
Halobacterium sp.
?
-
-
-
-
additional information
no substrates: methanol, glycerol
728288
Halobacterium sp. NRC-1
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 37300, calculated and SDS-PAGE
Halobacterium sp.
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
60
oxidation of alcohol
Halobacterium salinarum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reduction of aldehyde
Halobacterium salinarum
6
-
reduction of aldehydes
Halobacterium sp.
10
-
oxidation of alcohol
Halobacterium salinarum
10
-
oxidation of alcohols
Halobacterium sp.
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
dual cofactor dependency, NAD+ shows 60% of the activity with NADP+
Halobacterium salinarum
NADH
about 60% of the activity with NADPH
Halobacterium sp.
NADP+
dual cofactor dependency, NAD+ shows 60% of the activity with NADP+
Halobacterium salinarum
NADPH
preferred cofactor
Halobacterium sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
a hexahistidine-tagged recombinant version of the enzyme is heterologously overexpressed in Haloferax volcanii
Halobacterium salinarum
expresssion in Haloferax volcanii
Halobacterium sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
dual cofactor dependency, NAD+ shows 60% of the activity with NADP+
Halobacterium salinarum
NADH
about 60% of the activity with NADPH
Halobacterium sp.
NADP+
dual cofactor dependency, NAD+ shows 60% of the activity with NADP+
Halobacterium salinarum
NADPH
preferred cofactor
Halobacterium sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.05
-
NADPH
pH 6.0, 50C
Halobacterium salinarum
0.05
-
NADPH
pH 6.0, 50C
Halobacterium sp.
0.2
-
NADP+
pH 10.0, 50C
Halobacterium salinarum
0.2
-
NADP+
pH 6.0, 50C
Halobacterium sp.
3.4
-
acetaldehyde
pH 6.0, 50C
Halobacterium salinarum
3.4
-
acetaldehyde
pH 6.0, 50C
Halobacterium sp.
21.4
-
1-propanol
pH 10.0, 50C
Halobacterium salinarum
21.4
-
1-propanol
pH 6.0, 50C
Halobacterium sp.
107.9
-
ethanol
pH 10.0, 50C
Halobacterium salinarum
107.9
-
ethanol
pH 6.0, 50C
Halobacterium sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
KCl
a preference for KCl over NaCl is observed, with best activity at 4 M KCl
Halobacterium salinarum
KCl
maximum activity in presence of 4 M KCl
Halobacterium sp.
NaCl
the enzyme requires high salt concentrations for its activity, a preference for KCl over NaCl is observed
Halobacterium salinarum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37300
-
x * 37300, calculated and SDS-PAGE
Halobacterium sp.
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
acetonitrile
24 h at 4C, 13% loss of activity at 5% (v/v), 10% loss of activity at 10% (v/v), complete loss of activity at 30% (v/v)
Halobacterium salinarum
acetonitrile
10%, 90% residual activity
Halobacterium sp.
dimethyl sulfoxide
24 h at 4C, 11% loss of activity at 5% (v/v), 9% loss of activity at 10% (v/v), 59% loss of activity at 30% (v/v)
Halobacterium salinarum
DMSO
10%, 91% residual activity
Halobacterium sp.
Methanol
24 h at 4C, 8% loss of activity at 5% (v/v), 15% loss of activity at 10% (v/v), 94% loss of activity at 30% (v/v)
Halobacterium salinarum
Methanol
10%, 85% residual activity
Halobacterium sp.
tetrahydrofuran
24 h at 4C, 37% loss of activity at 5% (v/v), 57% loss of activity at 10% (v/v), complete loss of activity at 30% (v/v)
Halobacterium salinarum
tetrahydrofuran
10%, 43% residual activity
Halobacterium sp.
Purification (Commentary) (protein specific)
Commentary
Organism
purified in one step by immobilised Ni-affinity chromatography
Halobacterium salinarum
Storage Stability (protein specific)
Storage Stability
Organism
-20C, 30 days, 80% residual activity
Halobacterium sp.
-20C, 30 days, enzyme retains 80% of its original activity
Halobacterium salinarum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-butanol + NADP+
80% compared to activity with 1-propanol
728288
Halobacterium salinarum
butanal + NADPH + H+
-
-
-
?
1-butanol + NADP+
80% of the activtiy with 1-propanol
728288
Halobacterium sp.
butanal + NADPH + H+
-
-
-
?
1-butanol + NADP+
80% of the activtiy with 1-propanol
728288
Halobacterium sp. NRC-1
butanal + NADPH + H+
-
-
-
?
1-phenylethanol + NADP+
35% compared to activity with 1-propanol
728288
Halobacterium salinarum
acetophenone + NADPH + H+
-
-
-
?
1-phenylethanol + NADP+
35% of the activtiy with 1-propanol
728288
Halobacterium sp.
1-phenylethanone + NADPH + H+
-
-
-
?
1-propanol + NAD+
dual cofactor dependency, NAD+ shows 60% of the activity with NADP+
728288
Halobacterium salinarum
propanal + NADH + H+
-
-
-
?
1-propanol + NADP+
-
728288
Halobacterium sp.
propanal + NADPH + H+
-
-
-
?
1-propanol + NADP+
dual cofactor dependency, NAD+ shows 60% of the activity with NADP+
728288
Halobacterium salinarum
propanal + NADPH + H+
-
-
-
?
1-propanol + NADP+
-
728288
Halobacterium sp. NRC-1
propanal + NADPH + H+
-
-
-
?
2-butanol + NADP+
30% compared to activity with 1-propanol
728288
Halobacterium salinarum
butanone + NADPH + H+
-
-
-
?
2-butanol + NADP+
30% of the activtiy with 1-propanol
728288
Halobacterium sp.
butanone + NADPH + H+
-
-
-
?
2-propanol + NADP+
25% compared to activity with 1-propanol
728288
Halobacterium salinarum
acetone + NADPH + H+
-
-
-
?
acetaldehyde + NADPH + H+
-
728288
Halobacterium sp.
ethanol + NADP+
-
-
-
?
acetaldehyde + NADPH + H+
-
728288
Halobacterium sp. NRC-1
ethanol + NADP+
-
-
-
?
benzyl alcohol + NADP+
20% compared to activity with 1-propanol
728288
Halobacterium salinarum
benzaldehyde + NADPH + H+
-
-
-
?
ethanol + NADP+
85% compared to activity with 1-propanol
728288
Halobacterium salinarum
acetaldehyde + NADPH + H+
-
-
-
?
ethanol + NADP+
84% of the activtiy with 1-propanol
728288
Halobacterium sp.
acetaldehyde + NADPH + H+
-
-
-
?
ethanol + NADP+
84% of the activtiy with 1-propanol
728288
Halobacterium sp. NRC-1
acetaldehyde + NADPH + H+
-
-
-
?
additional information
preference for short-chain alcohol substrates, particularly for 1-propanol (100%) and ethanol (84%), no activity with glycerol or methanol
728288
Halobacterium salinarum
?
-
-
-
-
additional information
no substrates: methanol, glycerol
728288
Halobacterium sp.
?
-
-
-
-
additional information
no substrates: methanol, glycerol
728288
Halobacterium sp. NRC-1
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 37300, calculated and SDS-PAGE
Halobacterium sp.
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
55
60
oxidation of alcohol
Halobacterium salinarum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reduction of aldehyde
Halobacterium salinarum
6
-
reduction of aldehydes
Halobacterium sp.
10
-
oxidation of alcohol
Halobacterium salinarum
10
-
oxidation of alcohols
Halobacterium sp.
Other publictions for EC 1.1.1.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738540
Zheng
Cofactor specificity of the bi ...
Ruminiclostridium thermocellum, Thermoanaerobacterium saccharolyticum
J. Bacteriol.
197
2610-2619
2015
-
-
-
-
-
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
6
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
6
-
-
-
-
-
-
-
-
4
4
726699
Stekhanova
Sodium chloride-induced modula ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Biochem. Biotechnol.
171
1877-1889
2013
-
-
1
-
-
-
-
6
-
1
-
-
-
4
-
-
-
-
-
-
-
-
8
1
3
2
5
6
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
8
1
3
2
5
6
1
-
-
-
-
-
-
-
6
6
728288
Liliensiek
Heterologous overexpression, p ...
Halobacterium salinarum, Halobacterium sp., Halobacterium sp. NRC-1
Mol. Biotechnol.
55
143-149
2013
-
-
2
-
-
-
-
10
-
3
1
-
8
3
-
-
1
-
-
-
-
2
21
1
1
-
-
-
4
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
10
-
3
1
-
8
-
-
1
-
-
-
2
21
1
1
-
-
-
4
-
-
-
-
-
-
-
-
-
722208
Timpson
Characterization of alcohol de ...
Haloarcula marismortui
Extremophiles
16
57-66
2012
-
-
1
-
-
-
-
3
-
1
1
-
3
4
-
-
1
-
-
-
-
1
8
1
1
1
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
1
1
-
3
-
-
1
-
-
-
1
8
1
1
1
-
-
2
-
-
-
-
-
-
-
-
-
726769
Timpson
A comparison of two novel alco ...
Haloferax volcanii, Haloferax volcanii DSM 3757
Appl. Microbiol. Biotechnol.
97
195-203
2012
-
-
1
-
-
1
-
5
-
1
2
-
4
2
-
-
1
-
-
-
-
2
14
1
1
1
-
-
2
-
1
2
-
-
-
-
-
1
2
-
-
1
-
-
-
5
-
1
2
-
4
-
-
1
-
-
-
2
14
1
1
1
-
-
2
-
1
-
-
-
-
-
5
5
739526
Brown
Mutant alcohol dehydrogenase l ...
Ruminiclostridium thermocellum
Proc. Natl. Acad. Sci. USA
108
13752-71375
2011
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726606
Lyashenko
Expression, purification and c ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Acta Crystallogr. Sect. F
66
655-657
2010
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
726712
Stekhanova
Characterization of a thermost ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Environ. Microbiol.
76
4096-4098
2010
-
-
1
-
-
-
14
5
-
3
3
-
-
7
-
-
1
-
-
-
-
-
30
1
1
-
4
5
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
14
-
5
-
3
3
-
-
-
-
1
-
-
-
-
30
1
1
-
4
5
2
-
-
-
-
-
-
-
5
5
668112
Thomas
Retinoid metabolism in the rat ...
Rattus norvegicus
Br. J. Nutr.
93
59-63
2005
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657019
Montesano
A novel potato defence-related ...
Solanum tuberosum
Plant Mol. Biol.
52
177-189
2003
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15
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655563
Belyaeva
Human pancreas protein 2 (PAN2 ...
Homo sapiens
FEBS Lett.
531
489-493
2002
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1
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2
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287273
Hirschberg
N-terminal acetylation in a th ...
Gallus gallus
Cell. Mol. Life Sci.
58
1323-1326
2001
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287275
Korkhin
NADP-dependent bacterial alcoh ...
Clostridium beijerinckii, Thermoanaerobacter brockii
J. Mol. Biol.
278
967-981
1998
-
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2
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287277
Wales
NADP-dependent alcohol dehydro ...
Acinetobacter calcoaceticus, Bacillus subtilis, Bacillus subtilis NCIMB 3610, Escherichia coli, Escherichia coli ML30, Mycobacterium tuberculosis, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida NCIMB 9494, Rhodococcus rhodochrous, Rhodococcus rhodochrous NCIMB 13259, Rhodotorula graminis, Rhodotorula graminis KGX 39, Saccharomyces cerevisiae, Saccharomyces cerevisiae D273-10B, Streptomyces rimosus 4018, Streptomyces rimosus
Microbiology
140
173-183
1994
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6
17
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35
2
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10
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10
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6
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2
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4
2
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287278
Brophy
A novel NADPH/NADH-dependent a ...
Moniezia expansa
FEBS Lett.
263
305-307
1990
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287279
Hiu
Butanol-ethanol dehydrogenase ...
Clostridium beijerinckii
Appl. Environ. Microbiol.
53
697-703
1987
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287280
Hatanaka
-
Purification and properties of ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides IFO 3426
Agric. Biol. Chem.
38
1819-1833
1974
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287281
Rhodes
-
Co-factor specificity of plant ...
Cucumis melo
Phytochemistry
12
307-314
1973
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2
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287282
Fidge
The enzymatic reduction of ret ...
Rattus norvegicus
J. Biol. Chem.
243
4372-4379
1968
2
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