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Literature summary for 1.1.1.71 extracted from

  • Timpson, L.M.; Liliensiek, A.K.; Alsafadi, D.; Cassidy, J.; Sharkeym M.A.; Liddell, S.; Allers, T.; Paradisi, F.
    A comparison of two novel alcohol dehydrogenase enzymes (ADH1 and ADH2) from the extreme halophile Haloferax volcanii (2012), Appl. Microbiol. Biotechnol., 97, 195-203.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
homologously expressed Haloferax volcanii

General Stability

General Stability Organism
most stable when stored at pH 8.0 with 2 M and 3 M KCl Haloferax volcanii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
NADPH pH 6.0, temperature not specified in the publication Haloferax volcanii
0.2 1 NADP+ pH 10.0, temperature not specified in the publication Haloferax volcanii
6.7
-
1-butanol pH 10.0, temperature not specified in the publication Haloferax volcanii
9.2
-
acetaldehyde pH 6.0, temperature not specified in the publication Haloferax volcanii
44
-
ethanol pH 10.0, temperature not specified in the publication Haloferax volcanii

Metals/Ions

Metals/Ions Comment Organism Structure
KCl maximally active with ethanol with 4 M KCl. It is maximally active with 1-propanol with 2 M KCl and with 1-butanol and 1-pentanol with 1 M KCl. Optimum activity with the secondary alcohols, 2-propanol and 2-butanol, is observed with 3 M KCl and 2 M KCl, respectively, and with isoamyl alcohol in the presence of 1 M KCl. Maximum activity with benzyl alcohol is detected with 2 M KCl. Catalyzed the reductive reaction optimally at pH 6.0 with 1 M KCl Haloferax volcanii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
37800
-
4 * 37800, SDS-PAGE Haloferax volcanii
132000
-
gel filtration Haloferax volcanii

Organic Solvent Stability

Organic Solvent Comment Organism
acetonitrile 4°C, 72 h, retains 65% of its original activity following incubation with 5% (v/v) acetonitrile, retains 63% of its original activity following incubation with 10 (v/v) acetonitrile, complete inactivation following incubation with 30 (v/v) dimethyl sulfoxide Haloferax volcanii
dimethyl sulfoxide 4°C, 72 h, retains 74% of its original activity following incubation with 5% (v/v) dimethyl sulfoxide, retains 69% of its original activity following incubation with 10 (v/v) dimethyl sulfoxide, retains 47% of its original activity following incubation with 30 (v/v) dimethyl sulfoxide Haloferax volcanii
Glycerol stabilizing and cryoprotecting additive for the preservation of the enzyme over a short-time period, 10 days Haloferax volcanii
Methanol 4°C, 72 h, retains 70% of its original activity following incubation with 5% (v/v) acetonitrile, retains 67% of its original activity following incubation with 10 (v/v) acetonitrile, retains 39% of its original activity following incubation with 30 (v/v) dimethyl sulfoxide Haloferax volcanii

Organism

Organism UniProt Comment Textmining
Haloferax volcanii D4GP73
-
-
Haloferax volcanii DSM 3757 D4GP73
-
-

Purification (Commentary)

Purification (Comment) Organism
purified in one step by immobilized metal-affinity chromatography Haloferax volcanii

Storage Stability

Storage Stability Organism
-20°C, crude enzyme retains half of its original activity following incubation at -20°C for 75 days and almost one third of its original activity following incubation for 42 days Haloferax volcanii
4°C, inactive within 10 days Haloferax volcanii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-butanol + NADP+
-
Haloferax volcanii 1-butanal + NADPH + H+
-
?
1-pentanol + NADP+ about 75% of the activity with 1-butanol Haloferax volcanii pentanal + NADPH + H+
-
?
1-pentanol + NADP+ about 75% of the activity with 1-butanol Haloferax volcanii DSM 3757 pentanal + NADPH + H+
-
?
1-propanol + NADP+ about 60% of the activity with 1-butanol Haloferax volcanii propanal + NADPH + H+
-
?
1-propanol + NADP+ about 60% of the activity with 1-butanol Haloferax volcanii DSM 3757 propanal + NADPH + H+
-
?
2-butanol + NADP+ about 25% of the activity with 1-butanol Haloferax volcanii butanone + NADPH + H+
-
?
3-methylbutanal + NADP+ about% of the activity with 1-butanol Haloferax volcanii ? + NADPH + H+
-
?
benzyl alcohol + NADP+ about% of the activity with 1-butanol Haloferax volcanii benzaldehyde + NADPH + H+
-
?
benzyl alcohol + NADP+ about% of the activity with 1-butanol Haloferax volcanii DSM 3757 benzaldehyde + NADPH + H+
-
?
ethanol + NADP+ about 50% of the activity with 1-butanol Haloferax volcanii acetaldehyde + NADPH + H+
-
?
ethanol + NADP+ about 50% of the activity with 1-butanol Haloferax volcanii DSM 3757 acetaldehyde + NADPH + H+
-
?
isoamyl alcohol + NADP+ about 80% of the activity with 1-butanol Haloferax volcanii ? + NADPH + H+
-
?
additional information HvADH2 accepts a broad range of substrates, no activity with methanol Haloferax volcanii ?
-
?
additional information HvADH2 accepts a broad range of substrates, no activity with methanol Haloferax volcanii DSM 3757 ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 37800, SDS-PAGE Haloferax volcanii

Synonyms

Synonyms Comment Organism
HvADH2
-
Haloferax volcanii
HVO_B0071 locus name Haloferax volcanii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
85 90
-
Haloferax volcanii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
70 95 70°C: 60% of maximal activity, 95°C: 70% of maximal activity Haloferax volcanii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
reduction of aldehyde Haloferax volcanii
10
-
oxidation of alcohol Haloferax volcanii

pH Stability

pH Stability pH Stability Maximum Comment Organism
8
-
most stable when stored at pH 8.0 with 2 and 3 M KCl Haloferax volcanii

Cofactor

Cofactor Comment Organism Structure
NAD+ dual cofactor specificity, activity with NAD+ is 14% compared to the activity with NADP+ at pH 11.0 with 4 M KCl Haloferax volcanii
NADP+ dual cofactor specificity, activity with NAD+ is 14% compared to the activity with NADP+ at pH 11.0 with 4 M KCl Haloferax volcanii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.07
-
ethanol pH 10.0, temperature not specified in the publication Haloferax volcanii
0.479
-
1-butanol pH 10.0, temperature not specified in the publication Haloferax volcanii
0.744
-
acetaldehyde pH 6.0, temperature not specified in the publication Haloferax volcanii
12.39
-
NADP+ pH 10.0, temperature not specified in the publication Haloferax volcanii
242.8
-
NADPH pH 6.0, temperature not specified in the publication Haloferax volcanii