Cloned (Comment) | Organism |
---|---|
homologously expressed | Haloferax volcanii |
General Stability | Organism |
---|---|
most stable when stored at pH 8.0 with 2 M and 3 M KCl | Haloferax volcanii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.032 | - |
NADPH | pH 6.0, temperature not specified in the publication | Haloferax volcanii | |
0.2 | 1 | NADP+ | pH 10.0, temperature not specified in the publication | Haloferax volcanii | |
6.7 | - |
1-butanol | pH 10.0, temperature not specified in the publication | Haloferax volcanii | |
9.2 | - |
acetaldehyde | pH 6.0, temperature not specified in the publication | Haloferax volcanii | |
44 | - |
ethanol | pH 10.0, temperature not specified in the publication | Haloferax volcanii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | maximally active with ethanol with 4 M KCl. It is maximally active with 1-propanol with 2 M KCl and with 1-butanol and 1-pentanol with 1 M KCl. Optimum activity with the secondary alcohols, 2-propanol and 2-butanol, is observed with 3 M KCl and 2 M KCl, respectively, and with isoamyl alcohol in the presence of 1 M KCl. Maximum activity with benzyl alcohol is detected with 2 M KCl. Catalyzed the reductive reaction optimally at pH 6.0 with 1 M KCl | Haloferax volcanii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37800 | - |
4 * 37800, SDS-PAGE | Haloferax volcanii |
132000 | - |
gel filtration | Haloferax volcanii |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | 4°C, 72 h, retains 65% of its original activity following incubation with 5% (v/v) acetonitrile, retains 63% of its original activity following incubation with 10 (v/v) acetonitrile, complete inactivation following incubation with 30 (v/v) dimethyl sulfoxide | Haloferax volcanii |
dimethyl sulfoxide | 4°C, 72 h, retains 74% of its original activity following incubation with 5% (v/v) dimethyl sulfoxide, retains 69% of its original activity following incubation with 10 (v/v) dimethyl sulfoxide, retains 47% of its original activity following incubation with 30 (v/v) dimethyl sulfoxide | Haloferax volcanii |
Glycerol | stabilizing and cryoprotecting additive for the preservation of the enzyme over a short-time period, 10 days | Haloferax volcanii |
Methanol | 4°C, 72 h, retains 70% of its original activity following incubation with 5% (v/v) acetonitrile, retains 67% of its original activity following incubation with 10 (v/v) acetonitrile, retains 39% of its original activity following incubation with 30 (v/v) dimethyl sulfoxide | Haloferax volcanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloferax volcanii | D4GP73 | - |
- |
Haloferax volcanii DSM 3757 | D4GP73 | - |
- |
Purification (Comment) | Organism |
---|---|
purified in one step by immobilized metal-affinity chromatography | Haloferax volcanii |
Storage Stability | Organism |
---|---|
-20°C, crude enzyme retains half of its original activity following incubation at -20°C for 75 days and almost one third of its original activity following incubation for 42 days | Haloferax volcanii |
4°C, inactive within 10 days | Haloferax volcanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-butanol + NADP+ | - |
Haloferax volcanii | 1-butanal + NADPH + H+ | - |
? | |
1-pentanol + NADP+ | about 75% of the activity with 1-butanol | Haloferax volcanii | pentanal + NADPH + H+ | - |
? | |
1-pentanol + NADP+ | about 75% of the activity with 1-butanol | Haloferax volcanii DSM 3757 | pentanal + NADPH + H+ | - |
? | |
1-propanol + NADP+ | about 60% of the activity with 1-butanol | Haloferax volcanii | propanal + NADPH + H+ | - |
? | |
1-propanol + NADP+ | about 60% of the activity with 1-butanol | Haloferax volcanii DSM 3757 | propanal + NADPH + H+ | - |
? | |
2-butanol + NADP+ | about 25% of the activity with 1-butanol | Haloferax volcanii | butanone + NADPH + H+ | - |
? | |
3-methylbutanal + NADP+ | about% of the activity with 1-butanol | Haloferax volcanii | ? + NADPH + H+ | - |
? | |
benzyl alcohol + NADP+ | about% of the activity with 1-butanol | Haloferax volcanii | benzaldehyde + NADPH + H+ | - |
? | |
benzyl alcohol + NADP+ | about% of the activity with 1-butanol | Haloferax volcanii DSM 3757 | benzaldehyde + NADPH + H+ | - |
? | |
ethanol + NADP+ | about 50% of the activity with 1-butanol | Haloferax volcanii | acetaldehyde + NADPH + H+ | - |
? | |
ethanol + NADP+ | about 50% of the activity with 1-butanol | Haloferax volcanii DSM 3757 | acetaldehyde + NADPH + H+ | - |
? | |
isoamyl alcohol + NADP+ | about 80% of the activity with 1-butanol | Haloferax volcanii | ? + NADPH + H+ | - |
? | |
additional information | HvADH2 accepts a broad range of substrates, no activity with methanol | Haloferax volcanii | ? | - |
? | |
additional information | HvADH2 accepts a broad range of substrates, no activity with methanol | Haloferax volcanii DSM 3757 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 37800, SDS-PAGE | Haloferax volcanii |
Synonyms | Comment | Organism |
---|---|---|
HvADH2 | - |
Haloferax volcanii |
HVO_B0071 | locus name | Haloferax volcanii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | 90 | - |
Haloferax volcanii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 95 | 70°C: 60% of maximal activity, 95°C: 70% of maximal activity | Haloferax volcanii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of aldehyde | Haloferax volcanii |
10 | - |
oxidation of alcohol | Haloferax volcanii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
most stable when stored at pH 8.0 with 2 and 3 M KCl | Haloferax volcanii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | dual cofactor specificity, activity with NAD+ is 14% compared to the activity with NADP+ at pH 11.0 with 4 M KCl | Haloferax volcanii | |
NADP+ | dual cofactor specificity, activity with NAD+ is 14% compared to the activity with NADP+ at pH 11.0 with 4 M KCl | Haloferax volcanii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.07 | - |
ethanol | pH 10.0, temperature not specified in the publication | Haloferax volcanii | |
0.479 | - |
1-butanol | pH 10.0, temperature not specified in the publication | Haloferax volcanii | |
0.744 | - |
acetaldehyde | pH 6.0, temperature not specified in the publication | Haloferax volcanii | |
12.39 | - |
NADP+ | pH 10.0, temperature not specified in the publication | Haloferax volcanii | |
242.8 | - |
NADPH | pH 6.0, temperature not specified in the publication | Haloferax volcanii |