BRENDA - Enzyme Database show
show all sequences of 1.1.1.71

A comparison of two novel alcohol dehydrogenase enzymes (ADH1 and ADH2) from the extreme halophile Haloferax volcanii

Timpson, L.M.; Liliensiek, A.K.; Alsafadi, D.; Cassidy, J.; Sharkeym M.A.; Liddell, S.; Allers, T.; Paradisi, F.; Appl. Microbiol. Biotechnol. 97, 195-203 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
homologously expressed
Haloferax volcanii
General Stability
General Stability
Organism
most stable when stored at pH 8.0 with 2 M and 3 M KCl
Haloferax volcanii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.032
-
NADPH
pH 6.0, temperature not specified in the publication
Haloferax volcanii
0.2
1
NADP+
pH 10.0, temperature not specified in the publication
Haloferax volcanii
6.7
-
1-butanol
pH 10.0, temperature not specified in the publication
Haloferax volcanii
9.2
-
acetaldehyde
pH 6.0, temperature not specified in the publication
Haloferax volcanii
44
-
ethanol
pH 10.0, temperature not specified in the publication
Haloferax volcanii
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
KCl
maximally active with ethanol with 4 M KCl. It is maximally active with 1-propanol with 2 M KCl and with 1-butanol and 1-pentanol with 1 M KCl. Optimum activity with the secondary alcohols, 2-propanol and 2-butanol, is observed with 3 M KCl and 2 M KCl, respectively, and with isoamyl alcohol in the presence of 1 M KCl. Maximum activity with benzyl alcohol is detected with 2 M KCl. Catalyzed the reductive reaction optimally at pH 6.0 with 1 M KCl
Haloferax volcanii
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37800
-
4 * 37800, SDS-PAGE
Haloferax volcanii
132000
-
gel filtration
Haloferax volcanii
Organic Solvent Stability
Organic Solvent
Commentary
Organism
acetonitrile
4°C, 72 h, retains 65% of its original activity following incubation with 5% (v/v) acetonitrile, retains 63% of its original activity following incubation with 10 (v/v) acetonitrile, complete inactivation following incubation with 30 (v/v) dimethyl sulfoxide
Haloferax volcanii
dimethyl sulfoxide
4°C, 72 h, retains 74% of its original activity following incubation with 5% (v/v) dimethyl sulfoxide, retains 69% of its original activity following incubation with 10 (v/v) dimethyl sulfoxide, retains 47% of its original activity following incubation with 30 (v/v) dimethyl sulfoxide
Haloferax volcanii
Glycerol
stabilizing and cryoprotecting additive for the preservation of the enzyme over a short-time period, 10 days
Haloferax volcanii
Methanol
4°C, 72 h, retains 70% of its original activity following incubation with 5% (v/v) acetonitrile, retains 67% of its original activity following incubation with 10 (v/v) acetonitrile, retains 39% of its original activity following incubation with 30 (v/v) dimethyl sulfoxide
Haloferax volcanii
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Haloferax volcanii
D4GP73
-
-
Haloferax volcanii DSM 3757
D4GP73
-
-
Purification (Commentary)
Commentary
Organism
purified in one step by immobilized metal-affinity chromatography
Haloferax volcanii
Storage Stability
Storage Stability
Organism
-20°C, crude enzyme retains half of its original activity following incubation at -20°C for 75 days and almost one third of its original activity following incubation for 42 days
Haloferax volcanii
4°C, inactive within 10 days
Haloferax volcanii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-butanol + NADP+
-
726769
Haloferax volcanii
1-butanal + NADPH + H+
-
-
-
?
1-pentanol + NADP+
about 75% of the activity with 1-butanol
726769
Haloferax volcanii
pentanal + NADPH + H+
-
-
-
?
1-pentanol + NADP+
about 75% of the activity with 1-butanol
726769
Haloferax volcanii DSM 3757
pentanal + NADPH + H+
-
-
-
?
1-propanol + NADP+
about 60% of the activity with 1-butanol
726769
Haloferax volcanii
propanal + NADPH + H+
-
-
-
?
1-propanol + NADP+
about 60% of the activity with 1-butanol
726769
Haloferax volcanii DSM 3757
propanal + NADPH + H+
-
-
-
?
2-butanol + NADP+
about 25% of the activity with 1-butanol
726769
Haloferax volcanii
butanone + NADPH + H+
-
-
-
?
3-methylbutanal + NADP+
about% of the activity with 1-butanol
726769
Haloferax volcanii
? + NADPH + H+
-
-
-
?
benzyl alcohol + NADP+
about% of the activity with 1-butanol
726769
Haloferax volcanii
benzaldehyde + NADPH + H+
-
-
-
?
benzyl alcohol + NADP+
about% of the activity with 1-butanol
726769
Haloferax volcanii DSM 3757
benzaldehyde + NADPH + H+
-
-
-
?
ethanol + NADP+
about 50% of the activity with 1-butanol
726769
Haloferax volcanii
acetaldehyde + NADPH + H+
-
-
-
?
ethanol + NADP+
about 50% of the activity with 1-butanol
726769
Haloferax volcanii DSM 3757
acetaldehyde + NADPH + H+
-
-
-
?
isoamyl alcohol + NADP+
about 80% of the activity with 1-butanol
726769
Haloferax volcanii
? + NADPH + H+
-
-
-
?
additional information
HvADH2 accepts a broad range of substrates, no activity with methanol
726769
Haloferax volcanii
?
-
-
-
-
additional information
HvADH2 accepts a broad range of substrates, no activity with methanol
726769
Haloferax volcanii DSM 3757
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
tetramer
4 * 37800, SDS-PAGE
Haloferax volcanii
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
85
90
-
Haloferax volcanii
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
70
95
70°C: 60% of maximal activity, 95°C: 70% of maximal activity
Haloferax volcanii
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reduction of aldehyde
Haloferax volcanii
10
-
oxidation of alcohol
Haloferax volcanii
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
8
-
most stable when stored at pH 8.0 with 2 and 3 M KCl
Haloferax volcanii
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
dual cofactor specificity, activity with NAD+ is 14% compared to the activity with NADP+ at pH 11.0 with 4 M KCl
Haloferax volcanii
NADP+
dual cofactor specificity, activity with NAD+ is 14% compared to the activity with NADP+ at pH 11.0 with 4 M KCl
Haloferax volcanii
Cloned(Commentary) (protein specific)
Commentary
Organism
homologously expressed
Haloferax volcanii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
dual cofactor specificity, activity with NAD+ is 14% compared to the activity with NADP+ at pH 11.0 with 4 M KCl
Haloferax volcanii
NADP+
dual cofactor specificity, activity with NAD+ is 14% compared to the activity with NADP+ at pH 11.0 with 4 M KCl
Haloferax volcanii
General Stability (protein specific)
General Stability
Organism
most stable when stored at pH 8.0 with 2 M and 3 M KCl
Haloferax volcanii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.032
-
NADPH
pH 6.0, temperature not specified in the publication
Haloferax volcanii
0.2
1
NADP+
pH 10.0, temperature not specified in the publication
Haloferax volcanii
6.7
-
1-butanol
pH 10.0, temperature not specified in the publication
Haloferax volcanii
9.2
-
acetaldehyde
pH 6.0, temperature not specified in the publication
Haloferax volcanii
44
-
ethanol
pH 10.0, temperature not specified in the publication
Haloferax volcanii
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
KCl
maximally active with ethanol with 4 M KCl. It is maximally active with 1-propanol with 2 M KCl and with 1-butanol and 1-pentanol with 1 M KCl. Optimum activity with the secondary alcohols, 2-propanol and 2-butanol, is observed with 3 M KCl and 2 M KCl, respectively, and with isoamyl alcohol in the presence of 1 M KCl. Maximum activity with benzyl alcohol is detected with 2 M KCl. Catalyzed the reductive reaction optimally at pH 6.0 with 1 M KCl
Haloferax volcanii
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37800
-
4 * 37800, SDS-PAGE
Haloferax volcanii
132000
-
gel filtration
Haloferax volcanii
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
acetonitrile
4°C, 72 h, retains 65% of its original activity following incubation with 5% (v/v) acetonitrile, retains 63% of its original activity following incubation with 10 (v/v) acetonitrile, complete inactivation following incubation with 30 (v/v) dimethyl sulfoxide
Haloferax volcanii
dimethyl sulfoxide
4°C, 72 h, retains 74% of its original activity following incubation with 5% (v/v) dimethyl sulfoxide, retains 69% of its original activity following incubation with 10 (v/v) dimethyl sulfoxide, retains 47% of its original activity following incubation with 30 (v/v) dimethyl sulfoxide
Haloferax volcanii
Glycerol
stabilizing and cryoprotecting additive for the preservation of the enzyme over a short-time period, 10 days
Haloferax volcanii
Methanol
4°C, 72 h, retains 70% of its original activity following incubation with 5% (v/v) acetonitrile, retains 67% of its original activity following incubation with 10 (v/v) acetonitrile, retains 39% of its original activity following incubation with 30 (v/v) dimethyl sulfoxide
Haloferax volcanii
Purification (Commentary) (protein specific)
Commentary
Organism
purified in one step by immobilized metal-affinity chromatography
Haloferax volcanii
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, crude enzyme retains half of its original activity following incubation at -20°C for 75 days and almost one third of its original activity following incubation for 42 days
Haloferax volcanii
4°C, inactive within 10 days
Haloferax volcanii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-butanol + NADP+
-
726769
Haloferax volcanii
1-butanal + NADPH + H+
-
-
-
?
1-pentanol + NADP+
about 75% of the activity with 1-butanol
726769
Haloferax volcanii
pentanal + NADPH + H+
-
-
-
?
1-pentanol + NADP+
about 75% of the activity with 1-butanol
726769
Haloferax volcanii DSM 3757
pentanal + NADPH + H+
-
-
-
?
1-propanol + NADP+
about 60% of the activity with 1-butanol
726769
Haloferax volcanii
propanal + NADPH + H+
-
-
-
?
1-propanol + NADP+
about 60% of the activity with 1-butanol
726769
Haloferax volcanii DSM 3757
propanal + NADPH + H+
-
-
-
?
2-butanol + NADP+
about 25% of the activity with 1-butanol
726769
Haloferax volcanii
butanone + NADPH + H+
-
-
-
?
3-methylbutanal + NADP+
about% of the activity with 1-butanol
726769
Haloferax volcanii
? + NADPH + H+
-
-
-
?
benzyl alcohol + NADP+
about% of the activity with 1-butanol
726769
Haloferax volcanii
benzaldehyde + NADPH + H+
-
-
-
?
benzyl alcohol + NADP+
about% of the activity with 1-butanol
726769
Haloferax volcanii DSM 3757
benzaldehyde + NADPH + H+
-
-
-
?
ethanol + NADP+
about 50% of the activity with 1-butanol
726769
Haloferax volcanii
acetaldehyde + NADPH + H+
-
-
-
?
ethanol + NADP+
about 50% of the activity with 1-butanol
726769
Haloferax volcanii DSM 3757
acetaldehyde + NADPH + H+
-
-
-
?
isoamyl alcohol + NADP+
about 80% of the activity with 1-butanol
726769
Haloferax volcanii
? + NADPH + H+
-
-
-
?
additional information
HvADH2 accepts a broad range of substrates, no activity with methanol
726769
Haloferax volcanii
?
-
-
-
-
additional information
HvADH2 accepts a broad range of substrates, no activity with methanol
726769
Haloferax volcanii DSM 3757
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 37800, SDS-PAGE
Haloferax volcanii
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
85
90
-
Haloferax volcanii
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
70
95
70°C: 60% of maximal activity, 95°C: 70% of maximal activity
Haloferax volcanii
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reduction of aldehyde
Haloferax volcanii
10
-
oxidation of alcohol
Haloferax volcanii
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
8
-
most stable when stored at pH 8.0 with 2 and 3 M KCl
Haloferax volcanii
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.07
-
ethanol
pH 10.0, temperature not specified in the publication
Haloferax volcanii
0.479
-
1-butanol
pH 10.0, temperature not specified in the publication
Haloferax volcanii
0.744
-
acetaldehyde
pH 6.0, temperature not specified in the publication
Haloferax volcanii
12.39
-
NADP+
pH 10.0, temperature not specified in the publication
Haloferax volcanii
242.8
-
NADPH
pH 6.0, temperature not specified in the publication
Haloferax volcanii
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.07
-
ethanol
pH 10.0, temperature not specified in the publication
Haloferax volcanii
0.479
-
1-butanol
pH 10.0, temperature not specified in the publication
Haloferax volcanii
0.744
-
acetaldehyde
pH 6.0, temperature not specified in the publication
Haloferax volcanii
12.39
-
NADP+
pH 10.0, temperature not specified in the publication
Haloferax volcanii
242.8
-
NADPH
pH 6.0, temperature not specified in the publication
Haloferax volcanii
Other publictions for EC 1.1.1.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738540
Zheng
Cofactor specificity of the bi ...
Ruminiclostridium thermocellum, Thermoanaerobacterium saccharolyticum
J. Bacteriol.
197
2610-2619
2015
-
-
-
-
-
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
6
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
6
-
-
-
-
-
-
-
-
4
4
726699
Stekhanova
Sodium chloride-induced modula ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Biochem. Biotechnol.
171
1877-1889
2013
-
-
1
-
-
-
-
6
-
1
-
-
-
4
-
-
-
-
-
-
-
-
8
1
3
2
5
6
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
8
1
3
2
5
6
1
-
-
-
-
-
-
-
6
6
728288
Liliensiek
Heterologous overexpression, p ...
Halobacterium salinarum, Halobacterium sp., Halobacterium sp. NRC-1
Mol. Biotechnol.
55
143-149
2013
-
-
2
-
-
-
-
10
-
3
1
-
8
3
-
-
1
-
-
-
-
2
21
1
1
-
-
-
4
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
10
-
3
1
-
8
-
-
1
-
-
-
2
21
1
1
-
-
-
4
-
-
-
-
-
-
-
-
-
722208
Timpson
Characterization of alcohol de ...
Haloarcula marismortui
Extremophiles
16
57-66
2012
-
-
1
-
-
-
-
3
-
1
1
-
3
4
-
-
1
-
-
-
-
1
8
1
1
1
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
1
1
-
3
-
-
1
-
-
-
1
8
1
1
1
-
-
2
-
-
-
-
-
-
-
-
-
726769
Timpson
A comparison of two novel alco ...
Haloferax volcanii, Haloferax volcanii DSM 3757
Appl. Microbiol. Biotechnol.
97
195-203
2012
-
-
1
-
-
1
-
5
-
1
2
-
4
2
-
-
1
-
-
-
-
2
14
1
1
1
-
-
2
-
1
2
-
-
-
-
-
1
2
-
-
1
-
-
-
5
-
1
2
-
4
-
-
1
-
-
-
2
14
1
1
1
-
-
2
-
1
-
-
-
-
-
5
5
739526
Brown
Mutant alcohol dehydrogenase l ...
Ruminiclostridium thermocellum
Proc. Natl. Acad. Sci. USA
108
13752-71375
2011
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726606
Lyashenko
Expression, purification and c ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Acta Crystallogr. Sect. F
66
655-657
2010
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
726712
Stekhanova
Characterization of a thermost ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Environ. Microbiol.
76
4096-4098
2010
-
-
1
-
-
-
14
5
-
3
3
-
-
7
-
-
1
-
-
-
-
-
30
1
1
-
4
5
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
14
-
5
-
3
3
-
-
-
-
1
-
-
-
-
30
1
1
-
4
5
2
-
-
-
-
-
-
-
5
5
668112
Thomas
Retinoid metabolism in the rat ...
Rattus norvegicus
Br. J. Nutr.
93
59-63
2005
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
3
-
-
2
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
657019
Montesano
A novel potato defence-related ...
Solanum tuberosum
Plant Mol. Biol.
52
177-189
2003
2
-
1
-
-
-
-
5
-
-
-
1
-
1
-
-
1
1
-
1
-
-
15
-
-
-
-
-
-
-
-
2
-
-
-
2
-
1
2
-
-
-
-
-
-
5
-
-
-
1
-
-
-
1
-
1
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655563
Belyaeva
Human pancreas protein 2 (PAN2 ...
Homo sapiens
FEBS Lett.
531
489-493
2002
-
-
1
-
-
-
-
6
2
-
1
-
-
1
-
-
-
1
-
14
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
2
-
1
-
-
-
-
-
-
14
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287273
Hirschberg
N-terminal acetylation in a th ...
Gallus gallus
Cell. Mol. Life Sci.
58
1323-1326
2001
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287275
Korkhin
NADP-dependent bacterial alcoh ...
Clostridium beijerinckii, Thermoanaerobacter brockii
J. Mol. Biol.
278
967-981
1998
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287277
Wales
NADP-dependent alcohol dehydro ...
Acinetobacter calcoaceticus, Bacillus subtilis, Bacillus subtilis NCIMB 3610, Escherichia coli, Escherichia coli ML30, Mycobacterium tuberculosis, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida NCIMB 9494, Rhodococcus rhodochrous, Rhodococcus rhodochrous NCIMB 13259, Rhodotorula graminis, Rhodotorula graminis KGX 39, Saccharomyces cerevisiae, Saccharomyces cerevisiae D273-10B, Streptomyces rimosus 4018, Streptomyces rimosus
Microbiology
140
173-183
1994
-
-
-
-
-
-
6
17
-
-
2
-
-
20
-
-
2
2
-
-
-
-
35
2
-
-
-
13
4
2
-
10
-
-
-
-
-
-
10
-
-
-
-
6
-
17
-
-
2
-
-
-
-
2
-
-
-
-
35
2
-
-
-
13
4
2
-
-
-
-
-
-
-
-
287278
Brophy
A novel NADPH/NADH-dependent a ...
Moniezia expansa
FEBS Lett.
263
305-307
1990
-
-
-
-
-
-
2
2
1
-
-
-
-
1
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
2
1
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287279
Hiu
Butanol-ethanol dehydrogenase ...
Clostridium beijerinckii
Appl. Environ. Microbiol.
53
697-703
1987
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287280
Hatanaka
-
Purification and properties of ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides IFO 3426
Agric. Biol. Chem.
38
1819-1833
1974
-
-
-
-
-
-
9
8
-
-
-
-
-
2
-
-
1
-
-
-
-
-
14
-
-
-
1
-
2
-
1
1
-
-
-
-
-
-
1
-
-
-
-
9
-
8
-
-
-
-
-
-
-
1
-
-
-
-
14
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
287281
Rhodes
-
Co-factor specificity of plant ...
Cucumis melo
Phytochemistry
12
307-314
1973
-
-
-
-
-
-
2
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
4
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287282
Fidge
The enzymatic reduction of ret ...
Rattus norvegicus
J. Biol. Chem.
243
4372-4379
1968
2
-
-
-
-
-
5
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
14
-
-
-
-
-
1
1
-
1
-
-
-
2
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
14
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-