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show all sequences of 1.1.1.71

Characterization of a thermostable short-chain alcohol dehydrogenase from the hyperthermophilic archaeon Thermococcus sibiricus

Stekhanova, T.N.; Mardanov, A.V.; Bezsudnova, E.Y.; Gumerov, V.M.; Ravin, N.V.; Skryabin, K.G.; Popov, V.O.; Appl. Environ. Microbiol. 76, 4096-4098 (2010)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli; expression in Escherichia coli
Thermococcus sibiricus
Inhibitors
Inhibitors
Commentary
Organism
Structure
acetonitrile
complete inhibition in buffer without NaCl and in buffer with 600 mM NaCl
Thermococcus sibiricus
Chloroform
21% inhibition in buffer without NaCl, 19% inhibition in buffer with 600 mM NaCl
Thermococcus sibiricus
Dimethyl formamide
87% inhibition in buffer without NaCl, 59% inhibition in buffer with 600 mM NaCl
Thermococcus sibiricus
dimethyl sulfoxide
complete inhibition in buffer without NaCl, 60% inhibition in buffer with 600 mM NaCl
Thermococcus sibiricus
EDTA
5 mM, 20% inhibition; 5 mM, 80% of initial activity
Thermococcus sibiricus
ethyl acetate
complete inhibition in buffer without NaCl, 67% inhibition in buffer with 600 mM NaCl
Thermococcus sibiricus
Fe2+
10 mM, 74% of initial activity
Thermococcus sibiricus
FeSO4
10 mM, 26% inhibition
Thermococcus sibiricus
methanol
75% inhibition in buffer without NaCl, 81% inhibition in buffer with 600 mM NaCl
Thermococcus sibiricus
Mg2+
10 mM, 78% of initial activity
Thermococcus sibiricus
n-decane
9% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl
Thermococcus sibiricus
n-hexane
40% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl
Thermococcus sibiricus
Zn2+
10 mM, 79% of initial activity
Thermococcus sibiricus
ZnSO4
10 mM, 21% inhibition
Thermococcus sibiricus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NADPH
60°C, pH 7.5; pH 7.5, 60°C
Thermococcus sibiricus
0.022
-
NADP+
60°C, pH 10.5; pH 10.5, 60°C
Thermococcus sibiricus
17.75
-
Pyruvaldehyde
60°C, pH 7.5; pH 7.5, 60°C
Thermococcus sibiricus
54.4
-
D-xylose
60°C, pH 10.5; pH 10.5, 60°C
Thermococcus sibiricus
168
-
2-propanol
60°C, pH 10.5; pH 10.5, 60°C
Thermococcus sibiricus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
KCl
600 mM, 147% of initial activity; activity increases in the presence of KCl is maintained even at concentration of 3 M
Thermococcus sibiricus
additional information
metal independency is supported by the absence of a significant effect of TsAdh319 preincubation with 10 mM Me2+ for 30 min before measuring the activity in the presence of 1 mM Me2+ or EDTA
Thermococcus sibiricus
NaCl
400 mM, 206% of initial activity; activity increases in the presence of NaCl is maintained even at concentration of 4 M
Thermococcus sibiricus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26200
-
2 * 26200, calculated from sequence
Thermococcus sibiricus
28700
-
2 * 28700, SDS-PAGE
Thermococcus sibiricus
56000
60000
gel filtration
Thermococcus sibiricus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermococcus sibiricus
C6A190
-
-
Thermococcus sibiricus DSM 12597
C6A190
-
-
Purification (Commentary)
Commentary
Organism
-
Thermococcus sibiricus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-2-butanol + NADP+
196% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
butanone + NADPH
-
-
-
?
(S)-2-butanol + NADP+
196% of the activity with 2-propanol, 2fold preference for (S)-2-butanol over (RS)-2-butanol
726712
Thermococcus sibiricus
butanone + NADPH + H+
-
-
-
?
1,3-butanediol + NADP+
91% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
1-butanol + NADP+
80% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
butanal + NADPH
-
-
-
?
1-phenylmethanol + NADP+
180% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
benzaldehyde + NADPH + H+
-
-
-
?
2-pentanol + NADP+
67% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
2-pentanone + NADPH + H+
-
-
-
?
2-pentanol + NADP+
67% of the activity with 2-propanol
726712
Thermococcus sibiricus
pentan-2-one + NADPH + H+
-
-
-
?
2-propanol + NADP+
-
726712
Thermococcus sibiricus
acetone + NADPH
-
-
-
?
2-propanol + NADP+
-
726712
Thermococcus sibiricus
acetone + NADPH + H+
-
-
-
?
3-methyl-2-pentanone + NADPH + H+
13% relative activity as compared to pyruvaldehyde
726712
Thermococcus sibiricus
? + NADP+
-
-
-
?
benzyl alcohol + NADP+
180% of the activity with 2-propanol
726712
Thermococcus sibiricus
benzaldehyde + NADPH + H+
-
-
-
?
cellobiose + NADP+
71% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
cellobiose + NADP+
71% of the activity with 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
D-arabinose + NADP+
200% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
D-arabinose + NADP+
200% of the activity with 2-propanol. D-arabinose is preferred over L-arabinose
726712
Thermococcus sibiricus
D-arabitol + NADPH + H+
-
-
-
?
D-glucose + NADP+
146% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
D-glucose + NADP+
146% of the activity with 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
D-mannose + NADP+
48% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
D-ribose + NADP+
35% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
D-xylose + NADP+
246% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
D-xylose + NADP+
246% of the activity with 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
dimethylglyoxal + NADPH + H+
270% relative activity as compared to pyruvaldehyde
726712
Thermococcus sibiricus
? + NADP+
-
-
-
?
dimethylglyoxal + NADPH + H+
270% of the activity with pyruvaldehyde
726712
Thermococcus sibiricus
? + NADP+
-
-
-
?
ethanol + NADP+
36% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
acetaldehyde + NADPH + H+
-
-
-
?
glycerol + NADP+
16% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
glyceraldehyde + NADPH + H+
-
-
-
?
glyoxylic acid + NADPH + H+
36% relative activity as compared to pyruvaldehyde
726712
Thermococcus sibiricus
? + NADP+
-
-
-
?
L-arabinose + NADP+
17% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
additional information
no oxidation of methanol, methoxyethanol, ethylene glycol, or D-galactose. No reduction of acetone, cyclopentanone, D-arabinose, D-xylose, D-glucose or cellobiose
726712
Thermococcus sibiricus
?
-
-
-
-
pyruvaldehyde + NADPH + H+
-
726712
Thermococcus sibiricus
lactaldehyde + NADP+
-
-
-
?
pyruvaldehyde + NADPH + H+
-
726712
Thermococcus sibiricus
? + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 26200, calculated from sequence; 2 * 28700, SDS-PAGE
Thermococcus sibiricus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
assay at
Thermococcus sibiricus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
1.5 h, no loss of activity; 2 h, 10% loss of activity
Thermococcus sibiricus
80
-
1.5 h, no loss of activity; 2 h, 15% loss of activity
Thermococcus sibiricus
90
-
half-life 2 h; half-life: 2 h
Thermococcus sibiricus
100
-
half-life 1 h; half-life: 1 h
Thermococcus sibiricus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.45
-
NADP+
60°C, pH 10.5; pH 10.5, 60°C
Thermococcus sibiricus
0.53
-
2-propanol
60°C, pH 10.5; pH 10.5, 60°C
Thermococcus sibiricus
0.7
-
D-xylose
60°C, pH 10.5; pH 10.5, 60°C
Thermococcus sibiricus
1.51
-
NADPH
60°C, pH 7.5; pH 7.5, 60°C
Thermococcus sibiricus
2.04
-
Pyruvaldehyde
60°C, pH 7.5; pH 7.5, 60°C
Thermococcus sibiricus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at, reduction of ketones
Thermococcus sibiricus
10.5
-
assay at, oxidation of alcohols
Thermococcus sibiricus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Thermococcus sibiricus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli; expression in Escherichia coli
Thermococcus sibiricus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Thermococcus sibiricus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetonitrile
complete inhibition in buffer without NaCl and in buffer with 600 mM NaCl
Thermococcus sibiricus
Chloroform
21% inhibition in buffer without NaCl, 19% inhibition in buffer with 600 mM NaCl
Thermococcus sibiricus
Dimethyl formamide
87% inhibition in buffer without NaCl, 59% inhibition in buffer with 600 mM NaCl
Thermococcus sibiricus
dimethyl sulfoxide
complete inhibition in buffer without NaCl, 60% inhibition in buffer with 600 mM NaCl
Thermococcus sibiricus
EDTA
5 mM, 20% inhibition; 5 mM, 80% of initial activity
Thermococcus sibiricus
ethyl acetate
complete inhibition in buffer without NaCl, 67% inhibition in buffer with 600 mM NaCl
Thermococcus sibiricus
Fe2+
10 mM, 74% of initial activity
Thermococcus sibiricus
FeSO4
10 mM, 26% inhibition
Thermococcus sibiricus
methanol
75% inhibition in buffer without NaCl, 81% inhibition in buffer with 600 mM NaCl
Thermococcus sibiricus
Mg2+
10 mM, 78% of initial activity
Thermococcus sibiricus
n-decane
9% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl
Thermococcus sibiricus
n-hexane
40% inhibition in buffer without NaCl, slight activation in buffer with 600 mM NaCl
Thermococcus sibiricus
Zn2+
10 mM, 79% of initial activity
Thermococcus sibiricus
ZnSO4
10 mM, 21% inhibition
Thermococcus sibiricus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NADPH
60°C, pH 7.5; pH 7.5, 60°C
Thermococcus sibiricus
0.022
-
NADP+
60°C, pH 10.5; pH 10.5, 60°C
Thermococcus sibiricus
17.75
-
Pyruvaldehyde
60°C, pH 7.5; pH 7.5, 60°C
Thermococcus sibiricus
54.4
-
D-xylose
60°C, pH 10.5; pH 10.5, 60°C
Thermococcus sibiricus
168
-
2-propanol
60°C, pH 10.5; pH 10.5, 60°C
Thermococcus sibiricus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
KCl
600 mM, 147% of initial activity; activity increases in the presence of KCl is maintained even at concentration of 3 M
Thermococcus sibiricus
additional information
metal independency is supported by the absence of a significant effect of TsAdh319 preincubation with 10 mM Me2+ for 30 min before measuring the activity in the presence of 1 mM Me2+ or EDTA
Thermococcus sibiricus
NaCl
400 mM, 206% of initial activity; activity increases in the presence of NaCl is maintained even at concentration of 4 M
Thermococcus sibiricus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
26200
-
2 * 26200, calculated from sequence
Thermococcus sibiricus
28700
-
2 * 28700, SDS-PAGE
Thermococcus sibiricus
56000
60000
gel filtration
Thermococcus sibiricus
Purification (Commentary) (protein specific)
Commentary
Organism
-
Thermococcus sibiricus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(S)-2-butanol + NADP+
196% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
butanone + NADPH
-
-
-
?
(S)-2-butanol + NADP+
196% of the activity with 2-propanol, 2fold preference for (S)-2-butanol over (RS)-2-butanol
726712
Thermococcus sibiricus
butanone + NADPH + H+
-
-
-
?
1,3-butanediol + NADP+
91% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
1-butanol + NADP+
80% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
butanal + NADPH
-
-
-
?
1-phenylmethanol + NADP+
180% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
benzaldehyde + NADPH + H+
-
-
-
?
2-pentanol + NADP+
67% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
2-pentanone + NADPH + H+
-
-
-
?
2-pentanol + NADP+
67% of the activity with 2-propanol
726712
Thermococcus sibiricus
pentan-2-one + NADPH + H+
-
-
-
?
2-propanol + NADP+
-
726712
Thermococcus sibiricus
acetone + NADPH
-
-
-
?
2-propanol + NADP+
-
726712
Thermococcus sibiricus
acetone + NADPH + H+
-
-
-
?
3-methyl-2-pentanone + NADPH + H+
13% relative activity as compared to pyruvaldehyde
726712
Thermococcus sibiricus
? + NADP+
-
-
-
?
benzyl alcohol + NADP+
180% of the activity with 2-propanol
726712
Thermococcus sibiricus
benzaldehyde + NADPH + H+
-
-
-
?
cellobiose + NADP+
71% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
cellobiose + NADP+
71% of the activity with 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
D-arabinose + NADP+
200% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
D-arabinose + NADP+
200% of the activity with 2-propanol. D-arabinose is preferred over L-arabinose
726712
Thermococcus sibiricus
D-arabitol + NADPH + H+
-
-
-
?
D-glucose + NADP+
146% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
D-glucose + NADP+
146% of the activity with 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
D-mannose + NADP+
48% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
D-ribose + NADP+
35% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
D-xylose + NADP+
246% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
D-xylose + NADP+
246% of the activity with 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
dimethylglyoxal + NADPH + H+
270% relative activity as compared to pyruvaldehyde
726712
Thermococcus sibiricus
? + NADP+
-
-
-
?
dimethylglyoxal + NADPH + H+
270% of the activity with pyruvaldehyde
726712
Thermococcus sibiricus
? + NADP+
-
-
-
?
ethanol + NADP+
36% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
acetaldehyde + NADPH + H+
-
-
-
?
glycerol + NADP+
16% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
glyceraldehyde + NADPH + H+
-
-
-
?
glyoxylic acid + NADPH + H+
36% relative activity as compared to pyruvaldehyde
726712
Thermococcus sibiricus
? + NADP+
-
-
-
?
L-arabinose + NADP+
17% relative activity as compared to 2-propanol
726712
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
additional information
no oxidation of methanol, methoxyethanol, ethylene glycol, or D-galactose. No reduction of acetone, cyclopentanone, D-arabinose, D-xylose, D-glucose or cellobiose
726712
Thermococcus sibiricus
?
-
-
-
-
pyruvaldehyde + NADPH + H+
-
726712
Thermococcus sibiricus
lactaldehyde + NADP+
-
-
-
?
pyruvaldehyde + NADPH + H+
-
726712
Thermococcus sibiricus
? + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 26200, calculated from sequence; 2 * 28700, SDS-PAGE
Thermococcus sibiricus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
assay at
Thermococcus sibiricus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
70
-
1.5 h, no loss of activity; 2 h, 10% loss of activity
Thermococcus sibiricus
80
-
1.5 h, no loss of activity; 2 h, 15% loss of activity
Thermococcus sibiricus
90
-
half-life 2 h; half-life: 2 h
Thermococcus sibiricus
100
-
half-life 1 h; half-life: 1 h
Thermococcus sibiricus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.45
-
NADP+
60°C, pH 10.5; pH 10.5, 60°C
Thermococcus sibiricus
0.53
-
2-propanol
60°C, pH 10.5; pH 10.5, 60°C
Thermococcus sibiricus
0.7
-
D-xylose
60°C, pH 10.5; pH 10.5, 60°C
Thermococcus sibiricus
1.51
-
NADPH
60°C, pH 7.5; pH 7.5, 60°C
Thermococcus sibiricus
2.04
-
Pyruvaldehyde
60°C, pH 7.5; pH 7.5, 60°C
Thermococcus sibiricus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at, reduction of ketones
Thermococcus sibiricus
10.5
-
assay at, oxidation of alcohols
Thermococcus sibiricus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.003
-
2-propanol
60°C, pH 10.5
Thermococcus sibiricus
0.013
-
D-xylose
60°C, pH 10.5
Thermococcus sibiricus
0.11
-
Pyruvaldehyde
60°C, pH 7.5
Thermococcus sibiricus
20.45
-
NADP+
60°C, pH 10.5
Thermococcus sibiricus
75.5
-
NADPH
60°C, pH 7.5
Thermococcus sibiricus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.003
-
2-propanol
60°C, pH 10.5
Thermococcus sibiricus
0.013
-
D-xylose
60°C, pH 10.5
Thermococcus sibiricus
0.11
-
Pyruvaldehyde
60°C, pH 7.5
Thermococcus sibiricus
20.45
-
NADP+
60°C, pH 10.5
Thermococcus sibiricus
75.5
-
NADPH
60°C, pH 7.5
Thermococcus sibiricus
Other publictions for EC 1.1.1.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738540
Zheng
Cofactor specificity of the bi ...
Ruminiclostridium thermocellum, Thermoanaerobacterium saccharolyticum
J. Bacteriol.
197
2610-2619
2015
-
-
-
-
-
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
6
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
6
-
-
-
-
-
-
-
-
4
4
726699
Stekhanova
Sodium chloride-induced modula ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Biochem. Biotechnol.
171
1877-1889
2013
-
-
1
-
-
-
-
6
-
1
-
-
-
4
-
-
-
-
-
-
-
-
8
1
3
2
5
6
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
8
1
3
2
5
6
1
-
-
-
-
-
-
-
6
6
728288
Liliensiek
Heterologous overexpression, p ...
Halobacterium salinarum, Halobacterium sp., Halobacterium sp. NRC-1
Mol. Biotechnol.
55
143-149
2013
-
-
2
-
-
-
-
10
-
3
1
-
8
3
-
-
1
-
-
-
-
2
21
1
1
-
-
-
4
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
10
-
3
1
-
8
-
-
1
-
-
-
2
21
1
1
-
-
-
4
-
-
-
-
-
-
-
-
-
722208
Timpson
Characterization of alcohol de ...
Haloarcula marismortui
Extremophiles
16
57-66
2012
-
-
1
-
-
-
-
3
-
1
1
-
3
4
-
-
1
-
-
-
-
1
8
1
1
1
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
1
1
-
3
-
-
1
-
-
-
1
8
1
1
1
-
-
2
-
-
-
-
-
-
-
-
-
726769
Timpson
A comparison of two novel alco ...
Haloferax volcanii, Haloferax volcanii DSM 3757
Appl. Microbiol. Biotechnol.
97
195-203
2012
-
-
1
-
-
1
-
5
-
1
2
-
4
2
-
-
1
-
-
-
-
2
14
1
1
1
-
-
2
-
1
2
-
-
-
-
-
1
2
-
-
1
-
-
-
5
-
1
2
-
4
-
-
1
-
-
-
2
14
1
1
1
-
-
2
-
1
-
-
-
-
-
5
5
739526
Brown
Mutant alcohol dehydrogenase l ...
Ruminiclostridium thermocellum
Proc. Natl. Acad. Sci. USA
108
13752-71375
2011
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726606
Lyashenko
Expression, purification and c ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Acta Crystallogr. Sect. F
66
655-657
2010
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
726712
Stekhanova
Characterization of a thermost ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Environ. Microbiol.
76
4096-4098
2010
-
-
1
-
-
-
14
5
-
3
3
-
-
7
-
-
1
-
-
-
-
-
30
1
1
-
4
5
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
14
-
5
-
3
3
-
-
-
-
1
-
-
-
-
30
1
1
-
4
5
2
-
-
-
-
-
-
-
5
5
668112
Thomas
Retinoid metabolism in the rat ...
Rattus norvegicus
Br. J. Nutr.
93
59-63
2005
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
3
-
-
2
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
657019
Montesano
A novel potato defence-related ...
Solanum tuberosum
Plant Mol. Biol.
52
177-189
2003
2
-
1
-
-
-
-
5
-
-
-
1
-
1
-
-
1
1
-
1
-
-
15
-
-
-
-
-
-
-
-
2
-
-
-
2
-
1
2
-
-
-
-
-
-
5
-
-
-
1
-
-
-
1
-
1
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655563
Belyaeva
Human pancreas protein 2 (PAN2 ...
Homo sapiens
FEBS Lett.
531
489-493
2002
-
-
1
-
-
-
-
6
2
-
1
-
-
1
-
-
-
1
-
14
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
2
-
1
-
-
-
-
-
-
14
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287273
Hirschberg
N-terminal acetylation in a th ...
Gallus gallus
Cell. Mol. Life Sci.
58
1323-1326
2001
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287275
Korkhin
NADP-dependent bacterial alcoh ...
Clostridium beijerinckii, Thermoanaerobacter brockii
J. Mol. Biol.
278
967-981
1998
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287277
Wales
NADP-dependent alcohol dehydro ...
Acinetobacter calcoaceticus, Bacillus subtilis, Bacillus subtilis NCIMB 3610, Escherichia coli, Escherichia coli ML30, Mycobacterium tuberculosis, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida NCIMB 9494, Rhodococcus rhodochrous, Rhodococcus rhodochrous NCIMB 13259, Rhodotorula graminis, Rhodotorula graminis KGX 39, Saccharomyces cerevisiae, Saccharomyces cerevisiae D273-10B, Streptomyces rimosus 4018, Streptomyces rimosus
Microbiology
140
173-183
1994
-
-
-
-
-
-
6
17
-
-
2
-
-
20
-
-
2
2
-
-
-
-
35
2
-
-
-
13
4
2
-
10
-
-
-
-
-
-
10
-
-
-
-
6
-
17
-
-
2
-
-
-
-
2
-
-
-
-
35
2
-
-
-
13
4
2
-
-
-
-
-
-
-
-
287278
Brophy
A novel NADPH/NADH-dependent a ...
Moniezia expansa
FEBS Lett.
263
305-307
1990
-
-
-
-
-
-
2
2
1
-
-
-
-
1
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
2
1
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287279
Hiu
Butanol-ethanol dehydrogenase ...
Clostridium beijerinckii
Appl. Environ. Microbiol.
53
697-703
1987
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287280
Hatanaka
-
Purification and properties of ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides IFO 3426
Agric. Biol. Chem.
38
1819-1833
1974
-
-
-
-
-
-
9
8
-
-
-
-
-
2
-
-
1
-
-
-
-
-
14
-
-
-
1
-
2
-
1
1
-
-
-
-
-
-
1
-
-
-
-
9
-
8
-
-
-
-
-
-
-
1
-
-
-
-
14
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
287281
Rhodes
-
Co-factor specificity of plant ...
Cucumis melo
Phytochemistry
12
307-314
1973
-
-
-
-
-
-
2
4
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
4
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287282
Fidge
The enzymatic reduction of ret ...
Rattus norvegicus
J. Biol. Chem.
243
4372-4379
1968
2
-
-
-
-
-
5
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
14
-
-
-
-
-
1
1
-
1
-
-
-
2
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
14
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-