BRENDA - Enzyme Database show
show all sequences of 1.1.1.71

Sodium chloride-induced modulation of the activity and thermal stability of short-chain oxidoreductase from the archaeon Thermococcus sibiricus

Stekhanova, T.N.; Bezsudnova, E.Y.; Mardanov, A.V.; Gumerov, V.M.; Artemova, N.; Kleymenov, S.Y.; Popov, V.O.; Appl. Biochem. Biotechnol. 171, 1877-1889 (2013)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli as a fusion protein with (His)6-tag and 13 amino acid linker at the N-terminal
Thermococcus sibiricus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NADP+
60°C, pH 10.5, cosubstrate: 2,5-hexandiol
Thermococcus sibiricus
0.02
-
NADPH
60°C, pH 7.5, cosubstrate: pyruvaldehyde
Thermococcus sibiricus
1.5
-
2,3-Pentanedione
60°C, pH 7.5
Thermococcus sibiricus
13.1
-
(2S,5S)-2,5-hexanediol
60°C, pH 10.5
Thermococcus sibiricus
17.75
-
Pyruvaldehyde
60°C, pH 7.5
Thermococcus sibiricus
168
-
2-propanol
60°C, pH 10.5
Thermococcus sibiricus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
NaCl
activity is increased in the presence of NaCl and remains at the elevated level up to 4 M of NaCl. The rates of 2-propanol and 2,5-hexanediol oxidation are increased by more than 2fold after the addition of NaCl up to 1 M to the assay mixture and is retained at the increased level up to 4 M of NaCl
Thermococcus sibiricus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Thermococcus sibiricus
C6A190
-
-
Thermococcus sibiricus DSM 12597
C6A190
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S,5S)-2,5-hexanediol + NADP+
-
726699
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
(2S,5S)-2,5-hexanediol + NADP+
-
726699
Thermococcus sibiricus DSM 12597
? + NADPH + H+
-
-
-
?
2,3-pentanedione + NADPH + H+
-
726699
Thermococcus sibiricus
? + NADP+
-
-
-
?
2,3-pentanedione + NADPH + H+
-
726699
Thermococcus sibiricus DSM 12597
? + NADP+
-
-
-
?
2-propanol + NADP+
-
726699
Thermococcus sibiricus
acetone + NADPH + H+
-
-
-
?
2-propanol + NADP+
-
726699
Thermococcus sibiricus DSM 12597
acetone + NADPH + H+
-
-
-
?
pyruvaldehyde + NADPH + H+
-
726699
Thermococcus sibiricus
lactaldehyde + NADP+
-
-
-
?
pyruvaldehyde + NADPH + H+
-
726699
Thermococcus sibiricus DSM 12597
lactaldehyde + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
-
Thermococcus sibiricus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
assay at
Thermococcus sibiricus
70
-
oxidation of (2S,5S)-2,5-hexanediol, in presence of 1 M NaCl
Thermococcus sibiricus
83
-
oxidation of (2S,5S)-2,5-hexanediol, no addition of NaCl
Thermococcus sibiricus
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
50
75
50°C: about 50% of maximal activity, 75°C: about 60% of maximal activity, oxidation of (2S,5S)-2,5-hexanediol, in presence of 1 M NaCl
Thermococcus sibiricus
65
86
65°C: about 50% of maximal activity, 86°C: about 50% of maximal activity, oxidation of (2S,5S)-2,5-hexanediol, no addition of NaCl
Thermococcus sibiricus
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
thermal denaturation is fully irreversible and follows first-order kinetics
Thermococcus sibiricus
80
-
activity begins to decrease after 2 h preincubation. The rate of 2-propanol oxidation is decreased 34% and 58% over 24 h incubation at 80°C in the presence or absence of 1 M NaCl, respectively. Calculated half-life values of the enzyme in buffer with or without 1 M NaCl are 37 vs. 20 h at 80°C, respectively
Thermococcus sibiricus
90
-
activity begins to decrease after 30 min preincubation. The incubation at 90°C provokes 31% and 40% reduction of 2-propanol oxidation rate in the presence or absence of 1 M NaCl, respectively. Calculated half-life values of the enzyme in buffer with or without 1 M NaCl are 8 vs. 6 h at 90 °C, respectively
Thermococcus sibiricus
102
-
Tm-value in presence of 1 M NaCl
Thermococcus sibiricus
104
-
Tm-value in absence of NaCl
Thermococcus sibiricus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.53
-
2-propanol
60°C, pH 10.5
Thermococcus sibiricus
1.4
-
2,3-Pentanedione
60°C, pH 7.5
Thermococcus sibiricus
1.51
-
NADPH
60°C, pH 7.5, cosubstrate: pyruvaldehyde
Thermococcus sibiricus
2.02
-
NADP+
60°C, pH 10.5, cosubstrate: 2,5-hexandiol
Thermococcus sibiricus
2.04
-
Pyruvaldehyde
60°C, pH 7.5
Thermococcus sibiricus
2.2
-
(2S,5S)-2,5-hexanediol
60°C, pH 10.5
Thermococcus sibiricus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Thermococcus sibiricus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli as a fusion protein with (His)6-tag and 13 amino acid linker at the N-terminal
Thermococcus sibiricus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NADP+
60°C, pH 10.5, cosubstrate: 2,5-hexandiol
Thermococcus sibiricus
0.02
-
NADPH
60°C, pH 7.5, cosubstrate: pyruvaldehyde
Thermococcus sibiricus
1.5
-
2,3-Pentanedione
60°C, pH 7.5
Thermococcus sibiricus
13.1
-
(2S,5S)-2,5-hexanediol
60°C, pH 10.5
Thermococcus sibiricus
17.75
-
Pyruvaldehyde
60°C, pH 7.5
Thermococcus sibiricus
168
-
2-propanol
60°C, pH 10.5
Thermococcus sibiricus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
NaCl
activity is increased in the presence of NaCl and remains at the elevated level up to 4 M of NaCl. The rates of 2-propanol and 2,5-hexanediol oxidation are increased by more than 2fold after the addition of NaCl up to 1 M to the assay mixture and is retained at the increased level up to 4 M of NaCl
Thermococcus sibiricus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S,5S)-2,5-hexanediol + NADP+
-
726699
Thermococcus sibiricus
? + NADPH + H+
-
-
-
?
(2S,5S)-2,5-hexanediol + NADP+
-
726699
Thermococcus sibiricus DSM 12597
? + NADPH + H+
-
-
-
?
2,3-pentanedione + NADPH + H+
-
726699
Thermococcus sibiricus
? + NADP+
-
-
-
?
2,3-pentanedione + NADPH + H+
-
726699
Thermococcus sibiricus DSM 12597
? + NADP+
-
-
-
?
2-propanol + NADP+
-
726699
Thermococcus sibiricus
acetone + NADPH + H+
-
-
-
?
2-propanol + NADP+
-
726699
Thermococcus sibiricus DSM 12597
acetone + NADPH + H+
-
-
-
?
pyruvaldehyde + NADPH + H+
-
726699
Thermococcus sibiricus
lactaldehyde + NADP+
-
-
-
?
pyruvaldehyde + NADPH + H+
-
726699
Thermococcus sibiricus DSM 12597
lactaldehyde + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
-
Thermococcus sibiricus
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
60
-
assay at
Thermococcus sibiricus
70
-
oxidation of (2S,5S)-2,5-hexanediol, in presence of 1 M NaCl
Thermococcus sibiricus
83
-
oxidation of (2S,5S)-2,5-hexanediol, no addition of NaCl
Thermococcus sibiricus
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
50
75
50°C: about 50% of maximal activity, 75°C: about 60% of maximal activity, oxidation of (2S,5S)-2,5-hexanediol, in presence of 1 M NaCl
Thermococcus sibiricus
65
86
65°C: about 50% of maximal activity, 86°C: about 50% of maximal activity, oxidation of (2S,5S)-2,5-hexanediol, no addition of NaCl
Thermococcus sibiricus
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
additional information
-
thermal denaturation is fully irreversible and follows first-order kinetics
Thermococcus sibiricus
80
-
activity begins to decrease after 2 h preincubation. The rate of 2-propanol oxidation is decreased 34% and 58% over 24 h incubation at 80°C in the presence or absence of 1 M NaCl, respectively. Calculated half-life values of the enzyme in buffer with or without 1 M NaCl are 37 vs. 20 h at 80°C, respectively
Thermococcus sibiricus
90
-
activity begins to decrease after 30 min preincubation. The incubation at 90°C provokes 31% and 40% reduction of 2-propanol oxidation rate in the presence or absence of 1 M NaCl, respectively. Calculated half-life values of the enzyme in buffer with or without 1 M NaCl are 8 vs. 6 h at 90 °C, respectively
Thermococcus sibiricus
102
-
Tm-value in presence of 1 M NaCl
Thermococcus sibiricus
104
-
Tm-value in absence of NaCl
Thermococcus sibiricus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.53
-
2-propanol
60°C, pH 10.5
Thermococcus sibiricus
1.4
-
2,3-Pentanedione
60°C, pH 7.5
Thermococcus sibiricus
1.51
-
NADPH
60°C, pH 7.5, cosubstrate: pyruvaldehyde
Thermococcus sibiricus
2.02
-
NADP+
60°C, pH 10.5, cosubstrate: 2,5-hexandiol
Thermococcus sibiricus
2.04
-
Pyruvaldehyde
60°C, pH 7.5
Thermococcus sibiricus
2.2
-
(2S,5S)-2,5-hexanediol
60°C, pH 10.5
Thermococcus sibiricus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Thermococcus sibiricus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0032
-
2-propanol
60°C, pH 10.5
Thermococcus sibiricus
0.11
-
Pyruvaldehyde
60°C, pH 7.5
Thermococcus sibiricus
0.17
-
(2S,5S)-2,5-hexanediol
60°C, pH 10.5
Thermococcus sibiricus
1.33
-
2,3-Pentanedione
60°C, pH 7.5
Thermococcus sibiricus
76
-
NADPH
60°C, pH 7.5, cosubstrate: pyruvaldehyde
Thermococcus sibiricus
101
-
NADP+
60°C, pH 10.5, cosubstrate: 2,5-hexandiol
Thermococcus sibiricus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0032
-
2-propanol
60°C, pH 10.5
Thermococcus sibiricus
0.11
-
Pyruvaldehyde
60°C, pH 7.5
Thermococcus sibiricus
0.17
-
(2S,5S)-2,5-hexanediol
60°C, pH 10.5
Thermococcus sibiricus
1.33
-
2,3-Pentanedione
60°C, pH 7.5
Thermococcus sibiricus
76
-
NADPH
60°C, pH 7.5, cosubstrate: pyruvaldehyde
Thermococcus sibiricus
101
-
NADP+
60°C, pH 10.5, cosubstrate: 2,5-hexandiol
Thermococcus sibiricus
Other publictions for EC 1.1.1.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738540
Zheng
Cofactor specificity of the bi ...
Ruminiclostridium thermocellum, Thermoanaerobacterium saccharolyticum
J. Bacteriol.
197
2610-2619
2015
-
-
-
-
-
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
6
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
6
-
-
-
-
-
-
-
-
4
4
726699
Stekhanova
Sodium chloride-induced modula ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Biochem. Biotechnol.
171
1877-1889
2013
-
-
1
-
-
-
-
6
-
1
-
-
-
4
-
-
-
-
-
-
-
-
8
1
3
2
5
6
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
8
1
3
2
5
6
1
-
-
-
-
-
-
-
6
6
728288
Liliensiek
Heterologous overexpression, p ...
Halobacterium salinarum, Halobacterium sp., Halobacterium sp. NRC-1
Mol. Biotechnol.
55
143-149
2013
-
-
2
-
-
-
-
10
-
3
1
-
8
3
-
-
1
-
-
-
-
2
21
1
1
-
-
-
4
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
10
-
3
1
-
8
-
-
1
-
-
-
2
21
1
1
-
-
-
4
-
-
-
-
-
-
-
-
-
722208
Timpson
Characterization of alcohol de ...
Haloarcula marismortui
Extremophiles
16
57-66
2012
-
-
1
-
-
-
-
3
-
1
1
-
3
4
-
-
1
-
-
-
-
1
8
1
1
1
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
1
1
-
3
-
-
1
-
-
-
1
8
1
1
1
-
-
2
-
-
-
-
-
-
-
-
-
726769
Timpson
A comparison of two novel alco ...
Haloferax volcanii, Haloferax volcanii DSM 3757
Appl. Microbiol. Biotechnol.
97
195-203
2012
-
-
1
-
-
1
-
5
-
1
2
-
4
2
-
-
1
-
-
-
-
2
14
1
1
1
-
-
2
-
1
2
-
-
-
-
-
1
2
-
-
1
-
-
-
5
-
1
2
-
4
-
-
1
-
-
-
2
14
1
1
1
-
-
2
-
1
-
-
-
-
-
5
5
739526
Brown
Mutant alcohol dehydrogenase l ...
Ruminiclostridium thermocellum
Proc. Natl. Acad. Sci. USA
108
13752-71375
2011
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726606
Lyashenko
Expression, purification and c ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Acta Crystallogr. Sect. F
66
655-657
2010
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
726712
Stekhanova
Characterization of a thermost ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Environ. Microbiol.
76
4096-4098
2010
-
-
1
-
-
-
14
5
-
3
3
-
-
7
-
-
1
-
-
-
-
-
30
1
1
-
4
5
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
14
-
5
-
3
3
-
-
-
-
1
-
-
-
-
30
1
1
-
4
5
2
-
-
-
-
-
-
-
5
5
668112
Thomas
Retinoid metabolism in the rat ...
Rattus norvegicus
Br. J. Nutr.
93
59-63
2005
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
3
-
-
2
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
657019
Montesano
A novel potato defence-related ...
Solanum tuberosum
Plant Mol. Biol.
52
177-189
2003
2
-
1
-
-
-
-
5
-
-
-
1
-
1
-
-
1
1
-
1
-
-
15
-
-
-
-
-
-
-
-
2
-
-
-
2
-
1
2
-
-
-
-
-
-
5
-
-
-
1
-
-
-
1
-
1
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655563
Belyaeva
Human pancreas protein 2 (PAN2 ...
Homo sapiens
FEBS Lett.
531
489-493
2002
-
-
1
-
-
-
-
6
2
-
1
-
-
1
-
-
-
1
-
14
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
2
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1
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14
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2
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287273
Hirschberg
N-terminal acetylation in a th ...
Gallus gallus
Cell. Mol. Life Sci.
58
1323-1326
2001
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1
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3
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1
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1
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1
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1
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1
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1
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287275
Korkhin
NADP-dependent bacterial alcoh ...
Clostridium beijerinckii, Thermoanaerobacter brockii
J. Mol. Biol.
278
967-981
1998
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2
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2
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2
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2
2
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287277
Wales
NADP-dependent alcohol dehydro ...
Acinetobacter calcoaceticus, Bacillus subtilis, Bacillus subtilis NCIMB 3610, Escherichia coli, Escherichia coli ML30, Mycobacterium tuberculosis, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida NCIMB 9494, Rhodococcus rhodochrous, Rhodococcus rhodochrous NCIMB 13259, Rhodotorula graminis, Rhodotorula graminis KGX 39, Saccharomyces cerevisiae, Saccharomyces cerevisiae D273-10B, Streptomyces rimosus 4018, Streptomyces rimosus
Microbiology
140
173-183
1994
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6
17
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2
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20
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2
2
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35
2
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13
4
2
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10
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10
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6
-
17
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2
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2
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35
2
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13
4
2
-
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287278
Brophy
A novel NADPH/NADH-dependent a ...
Moniezia expansa
FEBS Lett.
263
305-307
1990
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2
2
1
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1
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1
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8
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1
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1
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2
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2
1
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1
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8
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287279
Hiu
Butanol-ethanol dehydrogenase ...
Clostridium beijerinckii
Appl. Environ. Microbiol.
53
697-703
1987
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2
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1
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1
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2
2
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1
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1
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2
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1
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2
2
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287280
Hatanaka
-
Purification and properties of ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides IFO 3426
Agric. Biol. Chem.
38
1819-1833
1974
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9
8
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2
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1
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14
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1
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2
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1
1
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1
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9
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8
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1
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14
-
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1
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2
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1
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287281
Rhodes
-
Co-factor specificity of plant ...
Cucumis melo
Phytochemistry
12
307-314
1973
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2
4
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1
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1
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1
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2
2
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1
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1
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2
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4
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1
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1
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2
2
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287282
Fidge
The enzymatic reduction of ret ...
Rattus norvegicus
J. Biol. Chem.
243
4372-4379
1968
2
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5
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1
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1
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14
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1
1
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1
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2
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1
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5
-
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1
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14
-
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1
1
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