BRENDA - Enzyme Database show
show all sequences of 1.1.1.71

Characterization of alcohol dehydrogenase (ADH12) from Haloarcula marismortui, an extreme halophile from the Dead Sea

Timpson, L.M.; Alsafadi, D.; Mac Donnchadha, C.; Liddell, S.; Sharkey, M.A.; Paradisi, F.; Extremophiles 16, 57-66 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
overexpression in Escherichia coli as a His-tagged protein
Haloarcula marismortui
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.029
-
NADPH
pH 6.0, 50C
Haloarcula marismortui
0.101
-
NADP+
pH 10.0, 50C
Haloarcula marismortui
5.1
-
acetaldehyde
pH 6.0, 50C
Haloarcula marismortui
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
K+
optimally active in the presence of 2 M KCl
Haloarcula marismortui
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41600
-
x * 41600, His-tagged protein, SDS-PAGE, mass spectrometry
Haloarcula marismortui
Organic Solvent Stability
Organic Solvent
Commentary
Organism
acetonitrile
10%, enzyme retains 69% of activity following incubation
Haloarcula marismortui
dimethyl sulfoxide
10%, enzyme retains 78% of activity following incubation
Haloarcula marismortui
tetrahydrofuran
10%, decativates
Haloarcula marismortui
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Haloarcula marismortui
Q5V676
-
-
Purification (Commentary)
Commentary
Organism
-
Haloarcula marismortui
Storage Stability
Storage Stability
Organism
-20C, retains approximately 80% of its original activity after 10 days
Haloarcula marismortui
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-butanol + NADP+
117% of the activity compared to ethanol
722208
Haloarcula marismortui
butanal + NADPH + H+
-
-
-
-
1-pentanol + NADP+
74% of the activity compared to ethanol
722208
Haloarcula marismortui
pentanal + NADPH + H+
-
-
-
-
1-propanol + NADP+
137% of the activity compared to ethanol
722208
Haloarcula marismortui
propanal + NADPH + H+
-
-
-
-
2-butanol + NADP+
43% of the activity compared to ethanol
722208
Haloarcula marismortui
2-butanone + NADPH + H+
-
-
-
-
acetaldehyde + NADPH + H+
-
722208
Haloarcula marismortui
ethanol + NADP+
-
-
-
?
ethanol + NAD+
enzyme appears to preferentially catalyze the reductive reaction. Activity with NAD+ is 75% less than that detected with NADP+
722208
Haloarcula marismortui
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NADP+
enzyme appears to preferentially catalyze the reductive reaction. Activity with NAD+ is 75% less than that detected with NADP+
722208
Haloarcula marismortui
acetaldehyde + NADPH + H+
-
-
-
?
additional information
the enzyme does not accept methanol, 2-propanol, glycerol or glucose as substrates
722208
Haloarcula marismortui
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 41600, His-tagged protein, SDS-PAGE, mass spectrometry
Haloarcula marismortui
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
60
-
-
Haloarcula marismortui
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
40
80
40C: about 50% of maximal activity, 80C: about 70% of maximal activity
Haloarcula marismortui
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reductive reaction
Haloarcula marismortui
10
-
oxidative reaction
Haloarcula marismortui
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
activity is 75% less than that detected with NADP+
Haloarcula marismortui
NADP+
preferred cofactor. Activity with NAD+ is 75% less than that detected with NADP+
Haloarcula marismortui
Cloned(Commentary) (protein specific)
Commentary
Organism
overexpression in Escherichia coli as a His-tagged protein
Haloarcula marismortui
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
activity is 75% less than that detected with NADP+
Haloarcula marismortui
NADP+
preferred cofactor. Activity with NAD+ is 75% less than that detected with NADP+
Haloarcula marismortui
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.029
-
NADPH
pH 6.0, 50C
Haloarcula marismortui
0.101
-
NADP+
pH 10.0, 50C
Haloarcula marismortui
5.1
-
acetaldehyde
pH 6.0, 50C
Haloarcula marismortui
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
K+
optimally active in the presence of 2 M KCl
Haloarcula marismortui
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41600
-
x * 41600, His-tagged protein, SDS-PAGE, mass spectrometry
Haloarcula marismortui
Organic Solvent Stability (protein specific)
Organic Solvent
Commentary
Organism
acetonitrile
10%, enzyme retains 69% of activity following incubation
Haloarcula marismortui
dimethyl sulfoxide
10%, enzyme retains 78% of activity following incubation
Haloarcula marismortui
tetrahydrofuran
10%, decativates
Haloarcula marismortui
Purification (Commentary) (protein specific)
Commentary
Organism
-
Haloarcula marismortui
Storage Stability (protein specific)
Storage Stability
Organism
-20C, retains approximately 80% of its original activity after 10 days
Haloarcula marismortui
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1-butanol + NADP+
117% of the activity compared to ethanol
722208
Haloarcula marismortui
butanal + NADPH + H+
-
-
-
-
1-pentanol + NADP+
74% of the activity compared to ethanol
722208
Haloarcula marismortui
pentanal + NADPH + H+
-
-
-
-
1-propanol + NADP+
137% of the activity compared to ethanol
722208
Haloarcula marismortui
propanal + NADPH + H+
-
-
-
-
2-butanol + NADP+
43% of the activity compared to ethanol
722208
Haloarcula marismortui
2-butanone + NADPH + H+
-
-
-
-
acetaldehyde + NADPH + H+
-
722208
Haloarcula marismortui
ethanol + NADP+
-
-
-
?
ethanol + NAD+
enzyme appears to preferentially catalyze the reductive reaction. Activity with NAD+ is 75% less than that detected with NADP+
722208
Haloarcula marismortui
acetaldehyde + NADH + H+
-
-
-
?
ethanol + NADP+
enzyme appears to preferentially catalyze the reductive reaction. Activity with NAD+ is 75% less than that detected with NADP+
722208
Haloarcula marismortui
acetaldehyde + NADPH + H+
-
-
-
?
additional information
the enzyme does not accept methanol, 2-propanol, glycerol or glucose as substrates
722208
Haloarcula marismortui
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 41600, His-tagged protein, SDS-PAGE, mass spectrometry
Haloarcula marismortui
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
60
-
-
Haloarcula marismortui
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
40
80
40C: about 50% of maximal activity, 80C: about 70% of maximal activity
Haloarcula marismortui
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
reductive reaction
Haloarcula marismortui
10
-
oxidative reaction
Haloarcula marismortui
Other publictions for EC 1.1.1.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738540
Zheng
Cofactor specificity of the bi ...
Ruminiclostridium thermocellum, Thermoanaerobacterium saccharolyticum
J. Bacteriol.
197
2610-2619
2015
-
-
-
-
-
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
6
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
6
-
-
-
-
-
-
-
-
4
4
726699
Stekhanova
Sodium chloride-induced modula ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Biochem. Biotechnol.
171
1877-1889
2013
-
-
1
-
-
-
-
6
-
1
-
-
-
4
-
-
-
-
-
-
-
-
8
1
3
2
5
6
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
8
1
3
2
5
6
1
-
-
-
-
-
-
-
6
6
728288
Liliensiek
Heterologous overexpression, p ...
Halobacterium salinarum, Halobacterium sp., Halobacterium sp. NRC-1
Mol. Biotechnol.
55
143-149
2013
-
-
2
-
-
-
-
10
-
3
1
-
8
3
-
-
1
-
-
-
-
2
21
1
1
-
-
-
4
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
10
-
3
1
-
8
-
-
1
-
-
-
2
21
1
1
-
-
-
4
-
-
-
-
-
-
-
-
-
722208
Timpson
Characterization of alcohol de ...
Haloarcula marismortui
Extremophiles
16
57-66
2012
-
-
1
-
-
-
-
3
-
1
1
-
3
4
-
-
1
-
-
-
-
1
8
1
1
1
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
1
1
-
3
-
-
1
-
-
-
1
8
1
1
1
-
-
2
-
-
-
-
-
-
-
-
-
726769
Timpson
A comparison of two novel alco ...
Haloferax volcanii, Haloferax volcanii DSM 3757
Appl. Microbiol. Biotechnol.
97
195-203
2012
-
-
1
-
-
1
-
5
-
1
2
-
4
2
-
-
1
-
-
-
-
2
14
1
1
1
-
-
2
-
1
2
-
-
-
-
-
1
2
-
-
1
-
-
-
5
-
1
2
-
4
-
-
1
-
-
-
2
14
1
1
1
-
-
2
-
1
-
-
-
-
-
5
5
739526
Brown
Mutant alcohol dehydrogenase l ...
Ruminiclostridium thermocellum
Proc. Natl. Acad. Sci. USA
108
13752-71375
2011
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726606
Lyashenko
Expression, purification and c ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Acta Crystallogr. Sect. F
66
655-657
2010
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
726712
Stekhanova
Characterization of a thermost ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Environ. Microbiol.
76
4096-4098
2010
-
-
1
-
-
-
14
5
-
3
3
-
-
7
-
-
1
-
-
-
-
-
30
1
1
-
4
5
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
14
-
5
-
3
3
-
-
-
-
1
-
-
-
-
30
1
1
-
4
5
2
-
-
-
-
-
-
-
5
5
668112
Thomas
Retinoid metabolism in the rat ...
Rattus norvegicus
Br. J. Nutr.
93
59-63
2005
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
3
-
-
2
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
657019
Montesano
A novel potato defence-related ...
Solanum tuberosum
Plant Mol. Biol.
52
177-189
2003
2
-
1
-
-
-
-
5
-
-
-
1
-
1
-
-
1
1
-
1
-
-
15
-
-
-
-
-
-
-
-
2
-
-
-
2
-
1
2
-
-
-
-
-
-
5
-
-
-
1
-
-
-
1
-
1
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655563
Belyaeva
Human pancreas protein 2 (PAN2 ...
Homo sapiens
FEBS Lett.
531
489-493
2002
-
-
1
-
-
-
-
6
2
-
1
-
-
1
-
-
-
1
-
14
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
2
-
1
-
-
-
-
-
-
14
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287273
Hirschberg
N-terminal acetylation in a th ...
Gallus gallus
Cell. Mol. Life Sci.
58
1323-1326
2001
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287275
Korkhin
NADP-dependent bacterial alcoh ...
Clostridium beijerinckii, Thermoanaerobacter brockii
J. Mol. Biol.
278
967-981
1998
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287277
Wales
NADP-dependent alcohol dehydro ...
Acinetobacter calcoaceticus, Bacillus subtilis, Bacillus subtilis NCIMB 3610, Escherichia coli, Escherichia coli ML30, Mycobacterium tuberculosis, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida NCIMB 9494, Rhodococcus rhodochrous, Rhodococcus rhodochrous NCIMB 13259, Rhodotorula graminis, Rhodotorula graminis KGX 39, Saccharomyces cerevisiae, Saccharomyces cerevisiae D273-10B, Streptomyces rimosus 4018, Streptomyces rimosus
Microbiology
140
173-183
1994
-
-
-
-
-
-
6
17
-
-
2
-
-
20
-
-
2
2
-
-
-
-
35
2
-
-
-
13
4
2
-
10
-
-
-
-
-
-
10
-
-
-
-
6
-
17
-
-
2
-
-
-
-
2
-
-
-
-
35
2
-
-
-
13
4
2
-
-
-
-
-
-
-
-
287278
Brophy
A novel NADPH/NADH-dependent a ...
Moniezia expansa
FEBS Lett.
263
305-307
1990
-
-
-
-
-
-
2
2
1
-
-
-
-
1
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
2
1
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287279
Hiu
Butanol-ethanol dehydrogenase ...
Clostridium beijerinckii
Appl. Environ. Microbiol.
53
697-703
1987
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
-
-
-
-
287280
Hatanaka
-
Purification and properties of ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides IFO 3426
Agric. Biol. Chem.
38
1819-1833
1974
-
-
-
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-
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8
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14
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1
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9
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8
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1
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14
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1
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287281
Rhodes
-
Co-factor specificity of plant ...
Cucumis melo
Phytochemistry
12
307-314
1973
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4
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2
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287282
Fidge
The enzymatic reduction of ret ...
Rattus norvegicus
J. Biol. Chem.
243
4372-4379
1968
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