BRENDA - Enzyme Database show
show all sequences of 1.1.1.71

A novel potato defence-related alcohol:NADP+ oxidoreductase induced in response to Erwinia carotovora

Montesano, M.; Hyytiainen, H.; Wettstein, R.; Palva, E.T.; Plant Mol. Biol. 52, 177-189 (2003)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
methyl jasmonate
-
Solanum tuberosum
salicylic acid
-
Solanum tuberosum
Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Solanum tuberosum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.8
-
2-Methoxybenzaldehyde
pH 6.5, 30°C
Solanum tuberosum
5.5
-
Hexanal
pH 6.5, 30°C
Solanum tuberosum
6.9
-
Salicylaldehyde
pH 6.5, 30°C
Solanum tuberosum
24.1
-
4-methoxybenzaldehyde
pH 6.5, 30°C
Solanum tuberosum
27.1
-
trans-2-hexenal
pH 6.5, 30°C
Solanum tuberosum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Solanum tuberosum
the enzyme plays an important role in potato defence response to Erwinia carotovora
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Solanum tuberosum
Q8H0L8
-
-
Purification (Commentary)
Commentary
Organism
isolation of a gene, that is induced after culture filtrate treatment from Erwinia carotovora
Solanum tuberosum
Reaction
Reaction
Commentary
Organism
a primary alcohol + NAD(P)+ = an aldehyde + NAD(P)H + H+
the defence-related alcohol dehydrogenase DRD-1 is an alcohol:NADP+ oxidoreductase with preference for various aromatic and aliphatic aldehydes
Solanum tuberosum
Source Tissue
Source Tissue
Commentary
Organism
Textmining
leaf
-
Solanum tuberosum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxy-3-methoxybenzaldehyde + NADPH + H+
-
657019
Solanum tuberosum
2-hydroxy-3-methoxybenzyl alcohol + NADP+
-
-
-
?
2-methoxybenzaldehyde + NADPH + H+
highest activity
657019
Solanum tuberosum
2-methoxybenzalcohol + NADP+
-
-
-
?
3,4-dimethoxybenzaldehyde + NADPH + H+
-
657019
Solanum tuberosum
3,4-dimethoxybenzyl alcohol + NADP+
-
-
-
?
4-methoxybenzaldehyde + NADPH + H+
-
657019
Solanum tuberosum
4-methoxybenzalcohol + NADP+
-
-
-
?
benzaldehyde + NADPH + H+
-
657019
Solanum tuberosum
benzyl alcohol + NADP+
-
-
-
?
cinnamaldehyde + NADPH + H+
-
657019
Solanum tuberosum
cinnamyl alcohol + NADP+
-
-
-
?
hexanal + NADPH + H+
-
657019
Solanum tuberosum
hexanol + NADP+
-
-
-
?
hydrocinnamaldehyde + NADPH + H+
-
657019
Solanum tuberosum
hydrocinnamyl alcohol + NADP+
-
-
-
?
additional information
the enzyme plays an important role in potato defence response to Erwinia carotovora
657019
Solanum tuberosum
?
-
-
-
-
additional information
not: conferyl aldehyde and sinapaldehyde, the enzyme exhibits an absolute requirement for NADPH as cofactor and can not be substituted by NADH
657019
Solanum tuberosum
?
-
-
-
-
octanal + NADPH + H+
-
657019
Solanum tuberosum
octanol + NADP+
-
-
-
?
salicylaldehye + NADPH + H+
-
657019
Solanum tuberosum
salicylalcohol + NADP+
-
-
-
?
trans-2,cis-6-nonadienal + NADPH + H+
-
657019
Solanum tuberosum
trans-2,cis-6-nonadienol + NADP+
-
-
-
?
trans-2-hexenal + NADPH + H+
-
657019
Solanum tuberosum
trans-2-hexenol + NADP+
-
-
-
?
trans-2-nonenal + NADPH + H+
-
657019
Solanum tuberosum
trans-2-nonenol + NADP+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Solanum tuberosum
NADPH
-
Solanum tuberosum
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
methyl jasmonate
-
Solanum tuberosum
salicylic acid
-
Solanum tuberosum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Solanum tuberosum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Solanum tuberosum
NADPH
-
Solanum tuberosum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.8
-
2-Methoxybenzaldehyde
pH 6.5, 30°C
Solanum tuberosum
5.5
-
Hexanal
pH 6.5, 30°C
Solanum tuberosum
6.9
-
Salicylaldehyde
pH 6.5, 30°C
Solanum tuberosum
24.1
-
4-methoxybenzaldehyde
pH 6.5, 30°C
Solanum tuberosum
27.1
-
trans-2-hexenal
pH 6.5, 30°C
Solanum tuberosum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Solanum tuberosum
the enzyme plays an important role in potato defence response to Erwinia carotovora
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
isolation of a gene, that is induced after culture filtrate treatment from Erwinia carotovora
Solanum tuberosum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
leaf
-
Solanum tuberosum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-hydroxy-3-methoxybenzaldehyde + NADPH + H+
-
657019
Solanum tuberosum
2-hydroxy-3-methoxybenzyl alcohol + NADP+
-
-
-
?
2-methoxybenzaldehyde + NADPH + H+
highest activity
657019
Solanum tuberosum
2-methoxybenzalcohol + NADP+
-
-
-
?
3,4-dimethoxybenzaldehyde + NADPH + H+
-
657019
Solanum tuberosum
3,4-dimethoxybenzyl alcohol + NADP+
-
-
-
?
4-methoxybenzaldehyde + NADPH + H+
-
657019
Solanum tuberosum
4-methoxybenzalcohol + NADP+
-
-
-
?
benzaldehyde + NADPH + H+
-
657019
Solanum tuberosum
benzyl alcohol + NADP+
-
-
-
?
cinnamaldehyde + NADPH + H+
-
657019
Solanum tuberosum
cinnamyl alcohol + NADP+
-
-
-
?
hexanal + NADPH + H+
-
657019
Solanum tuberosum
hexanol + NADP+
-
-
-
?
hydrocinnamaldehyde + NADPH + H+
-
657019
Solanum tuberosum
hydrocinnamyl alcohol + NADP+
-
-
-
?
additional information
the enzyme plays an important role in potato defence response to Erwinia carotovora
657019
Solanum tuberosum
?
-
-
-
-
additional information
not: conferyl aldehyde and sinapaldehyde, the enzyme exhibits an absolute requirement for NADPH as cofactor and can not be substituted by NADH
657019
Solanum tuberosum
?
-
-
-
-
octanal + NADPH + H+
-
657019
Solanum tuberosum
octanol + NADP+
-
-
-
?
salicylaldehye + NADPH + H+
-
657019
Solanum tuberosum
salicylalcohol + NADP+
-
-
-
?
trans-2,cis-6-nonadienal + NADPH + H+
-
657019
Solanum tuberosum
trans-2,cis-6-nonadienol + NADP+
-
-
-
?
trans-2-hexenal + NADPH + H+
-
657019
Solanum tuberosum
trans-2-hexenol + NADP+
-
-
-
?
trans-2-nonenal + NADPH + H+
-
657019
Solanum tuberosum
trans-2-nonenol + NADP+
-
-
-
?
Other publictions for EC 1.1.1.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738540
Zheng
Cofactor specificity of the bi ...
Ruminiclostridium thermocellum, Thermoanaerobacterium saccharolyticum
J. Bacteriol.
197
2610-2619
2015
-
-
-
-
-
-
-
5
-
-
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
-
6
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
6
-
-
-
-
-
-
-
-
4
4
726699
Stekhanova
Sodium chloride-induced modula ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Biochem. Biotechnol.
171
1877-1889
2013
-
-
1
-
-
-
-
6
-
1
-
-
-
4
-
-
-
-
-
-
-
-
8
1
3
2
5
6
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
-
1
-
-
-
-
-
-
-
-
-
-
8
1
3
2
5
6
1
-
-
-
-
-
-
-
6
6
728288
Liliensiek
Heterologous overexpression, p ...
Halobacterium salinarum, Halobacterium sp., Halobacterium sp. NRC-1
Mol. Biotechnol.
55
143-149
2013
-
-
2
-
-
-
-
10
-
3
1
-
8
3
-
-
1
-
-
-
-
2
21
1
1
-
-
-
4
-
-
4
-
-
-
-
-
2
4
-
-
-
-
-
-
10
-
3
1
-
8
-
-
1
-
-
-
2
21
1
1
-
-
-
4
-
-
-
-
-
-
-
-
-
722208
Timpson
Characterization of alcohol de ...
Haloarcula marismortui
Extremophiles
16
57-66
2012
-
-
1
-
-
-
-
3
-
1
1
-
3
4
-
-
1
-
-
-
-
1
8
1
1
1
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
3
-
1
1
-
3
-
-
1
-
-
-
1
8
1
1
1
-
-
2
-
-
-
-
-
-
-
-
-
726769
Timpson
A comparison of two novel alco ...
Haloferax volcanii, Haloferax volcanii DSM 3757
Appl. Microbiol. Biotechnol.
97
195-203
2012
-
-
1
-
-
1
-
5
-
1
2
-
4
2
-
-
1
-
-
-
-
2
14
1
1
1
-
-
2
-
1
2
-
-
-
-
-
1
2
-
-
1
-
-
-
5
-
1
2
-
4
-
-
1
-
-
-
2
14
1
1
1
-
-
2
-
1
-
-
-
-
-
5
5
739526
Brown
Mutant alcohol dehydrogenase l ...
Ruminiclostridium thermocellum
Proc. Natl. Acad. Sci. USA
108
13752-71375
2011
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
726606
Lyashenko
Expression, purification and c ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Acta Crystallogr. Sect. F
66
655-657
2010
-
-
1
1
-
-
-
-
-
-
-
-
-
4
-
-
1
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
726712
Stekhanova
Characterization of a thermost ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Environ. Microbiol.
76
4096-4098
2010
-
-
1
-
-
-
14
5
-
3
3
-
-
7
-
-
1
-
-
-
-
-
30
1
1
-
4
5
2
-
-
1
-
-
-
-
-
1
1
-
-
-
-
14
-
5
-
3
3
-
-
-
-
1
-
-
-
-
30
1
1
-
4
5
2
-
-
-
-
-
-
-
5
5
668112
Thomas
Retinoid metabolism in the rat ...
Rattus norvegicus
Br. J. Nutr.
93
59-63
2005
-
-
-
-
-
-
-
-
1
-
-
1
-
2
-
-
-
-
-
3
-
-
2
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
3
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
657019
Montesano
A novel potato defence-related ...
Solanum tuberosum
Plant Mol. Biol.
52
177-189
2003
2
-
1
-
-
-
-
5
-
-
-
1
-
1
-
-
1
1
-
1
-
-
15
-
-
-
-
-
-
-
-
2
-
-
-
2
-
1
2
-
-
-
-
-
-
5
-
-
-
1
-
-
-
1
-
1
-
-
15
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
655563
Belyaeva
Human pancreas protein 2 (PAN2 ...
Homo sapiens
FEBS Lett.
531
489-493
2002
-
-
1
-
-
-
-
6
2
-
1
-
-
1
-
-
-
1
-
14
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
6
2
-
1
-
-
-
-
-
-
14
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287273
Hirschberg
N-terminal acetylation in a th ...
Gallus gallus
Cell. Mol. Life Sci.
58
1323-1326
2001
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
1
-
1
-
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-
1
-
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-
-
-
-
-
-
-
-
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-
-
-
-
-
-
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-
1
-
-
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-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287275
Korkhin
NADP-dependent bacterial alcoh ...
Clostridium beijerinckii, Thermoanaerobacter brockii
J. Mol. Biol.
278
967-981
1998
-
-
-
2
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
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-
-
2
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287277
Wales
NADP-dependent alcohol dehydro ...
Acinetobacter calcoaceticus, Bacillus subtilis, Bacillus subtilis NCIMB 3610, Escherichia coli, Escherichia coli ML30, Mycobacterium tuberculosis, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida NCIMB 9494, Rhodococcus rhodochrous, Rhodococcus rhodochrous NCIMB 13259, Rhodotorula graminis, Rhodotorula graminis KGX 39, Saccharomyces cerevisiae, Saccharomyces cerevisiae D273-10B, Streptomyces rimosus 4018, Streptomyces rimosus
Microbiology
140
173-183
1994
-
-
-
-
-
-
6
17
-
-
2
-
-
20
-
-
2
2
-
-
-
-
35
2
-
-
-
13
4
2
-
10
-
-
-
-
-
-
10
-
-
-
-
6
-
17
-
-
2
-
-
-
-
2
-
-
-
-
35
2
-
-
-
13
4
2
-
-
-
-
-
-
-
-
287278
Brophy
A novel NADPH/NADH-dependent a ...
Moniezia expansa
FEBS Lett.
263
305-307
1990
-
-
-
-
-
-
2
2
1
-
-
-
-
1
-
-
1
-
-
-
-
-
8
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
2
1
-
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287279
Hiu
Butanol-ethanol dehydrogenase ...
Clostridium beijerinckii
Appl. Environ. Microbiol.
53
697-703
1987
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
-
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287280
Hatanaka
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Purification and properties of ...
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1974
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-
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9
8
-
-
-
-
-
2
-
-
1
-
-
-
-
-
14
-
-
-
1
-
2
-
1
1
-
-
-
-
-
-
1
-
-
-
-
9
-
8
-
-
-
-
-
-
-
1
-
-
-
-
14
-
-
-
1
-
2
-
1
-
-
-
-
-
-
-
287281
Rhodes
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Co-factor specificity of plant ...
Cucumis melo
Phytochemistry
12
307-314
1973
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-
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-
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2
4
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1
-
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1
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-
-
-
-
1
-
-
-
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
4
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
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2
2
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-
287282
Fidge
The enzymatic reduction of ret ...
Rattus norvegicus
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2
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5
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1
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1
-
-
-
-
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14
-
-
-
-
-
1
1
-
1
-
-
-
2
-
-
1
-
-
-
-
5
-
-
-
-
-
-
-
-
-
1
-
-
-
-
14
-
-
-
-
-
1
1
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-
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-
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-