BRENDA - Enzyme Database show
show all sequences of 1.1.1.71

NADP-dependent alcohol dehydrogenases in bacteria and yeast: purification and partial characterization of the enzymes from Acinetobacter calcoaceticus and Saccharomyces cerevisiae

Wales, M.R.; Fewson, C.A.; Microbiology 140, 173-183 (1994)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
4-chloromercuribenzoate
0.01 mM, complete inhibition
Acinetobacter calcoaceticus
4-chloromercuribenzoate
0.01 mM, complete inhibition; some protection of partial inhibition resulting from 0.001 mM 4-chloromercuribenzoate in the presence of 1 mM NADP+, almost complete protection with 10 mM hexan-1-ol
Saccharomyces cerevisiae
EDTA
10 mM, 20% inhibition
Saccharomyces cerevisiae
iodoacetamide
1 mM, complete inhibition
Saccharomyces cerevisiae
N-ethylmaleimide
1 mM, complete inhibition
Saccharomyces cerevisiae
Sodium azide
10 mM, 40% inhibition
Saccharomyces cerevisiae
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00079
-
NADPH
-
Saccharomyces cerevisiae
0.00433
-
NADPH
-
Acinetobacter calcoaceticus
0.0202
-
NADP+
-
Saccharomyces cerevisiae
0.038
-
NADP+
-
Acinetobacter calcoaceticus
0.75
-
Butanal
-
Saccharomyces cerevisiae
1.09
-
heptan-1-ol
-
Acinetobacter calcoaceticus
1.22
-
hexan-1-ol
-
Acinetobacter calcoaceticus
3.97
-
Butanal
-
Acinetobacter calcoaceticus
5.8
-
pentan-1-ol
-
Acinetobacter calcoaceticus
6.2
-
octan-1-ol
-
Saccharomyces cerevisiae
6.8
-
hexan-1-ol
-
Saccharomyces cerevisiae
14.1
-
pentan-1-ol
-
Saccharomyces cerevisiae
52.7
-
butan-1-ol
-
Acinetobacter calcoaceticus
78.6
-
butan-1-ol
-
Saccharomyces cerevisiae
150
-
benzyl alcohol
-
Acinetobacter calcoaceticus
727
-
ethanol
-
Acinetobacter calcoaceticus
4790
-
ethanol
-
Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40300
-
4 * 40300, SDS-PAGE
Acinetobacter calcoaceticus
43500
-
1 * 43500, SDS-PAGE
Saccharomyces cerevisiae
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Acinetobacter calcoaceticus
-
strains NCIB 8250 and HO1-N
-
Bacillus subtilis
-
strain NCIMB 3610
-
Bacillus subtilis NCIMB 3610
-
strain NCIMB 3610
-
Escherichia coli
-
strain ML30
-
Escherichia coli ML30
-
strain ML30
-
Mycobacterium tuberculosis
-
var. bosis
-
Pseudomonas aeruginosa
-
strains NCIMB 10548 and 196A
-
Pseudomonas putida
-
NCIMB 9494
-
Pseudomonas putida NCIMB 9494
-
NCIMB 9494
-
Rhodococcus rhodochrous
-
strain NCIMB 13259
-
Rhodococcus rhodochrous NCIMB 13259
-
strain NCIMB 13259
-
Rhodotorula graminis
-
strain KGX 39
-
Rhodotorula graminis KGX 39
-
strain KGX 39
-
Saccharomyces cerevisiae
-
D273-10B
-
Saccharomyces cerevisiae D273-10B
-
D273-10B
-
Streptomyces rimosus
-
strain 4018
-
Streptomyces rimosus 4018
-
strain 4018
-
Purification (Commentary)
Commentary
Organism
DEAE-Sephacel, ammonium sulfate, Sephacryl S300HR, Phenyl-Sepharose, Red A
Saccharomyces cerevisiae
DEAE-Sephacel, CM-cellulose, Sephacryl S300HR, Phenyl-Sepharose, Blue A/DEAE-Sephacel
Acinetobacter calcoaceticus
Reaction
Reaction
Commentary
Organism
a primary alcohol + NAD(P)+ = an aldehyde + NAD(P)H + H+
enzyme is stereospecific for the pro-R hydride of NADPH
Acinetobacter calcoaceticus
a primary alcohol + NAD(P)+ = an aldehyde + NAD(P)H + H+
enzyme is stereospecific for the pro-R hydride of NADPH
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
allyl alcohol + NADP+
28% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
acrylaldehyde + NADPH
-
287277
Acinetobacter calcoaceticus
r
benzyl alcohol + NADP+
-
287277
Acinetobacter calcoaceticus
benzaldehyde + NADPH
-
287277
Acinetobacter calcoaceticus
r
butan-1,4-diol + NADP+
29% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
butandialdehyde + NADPH
-
287277
Acinetobacter calcoaceticus
r
butan-1-ol + NADP+
36.3% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
butanal + NADPH
-
287277
Acinetobacter calcoaceticus
r
cis-hex-3-en-1-ol + NADP+
18.7% of activity with hexan-1ol
287277
Acinetobacter calcoaceticus
cis-hex-3-en-1-al + NADPH
-
287277
Acinetobacter calcoaceticus
r
decan-1-ol + NADP+
decrease in enzyme activity with increase in chain length
287277
Acinetobacter calcoaceticus
decanal + NADPH
-
287277
Acinetobacter calcoaceticus
r
dodecan-1-ol + NADP+
decrease in enzyme activity with increase in chain length
287277
Acinetobacter calcoaceticus
dodecanal + NADPH
-
287277
Acinetobacter calcoaceticus
r
ethanediol + NADP+
8.5% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
glyoxal + NADPH
-
287277
Acinetobacter calcoaceticus
r
ethanol + NADP+
8.5% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
ethanal + NADPH + H+
-
287277
Acinetobacter calcoaceticus
r
heptan-1-ol + NADP+
-
287277
Acinetobacter calcoaceticus
heptanal + NADPH
-
287277
Acinetobacter calcoaceticus
r
hexan-1-ol + NADP+
-
287277
Bacillus subtilis
hexanal + NADPH
-
287277
Bacillus subtilis
?
hexan-1-ol + NADP+
-
287277
Escherichia coli
hexanal + NADPH
-
287277
Escherichia coli
?
hexan-1-ol + NADP+
-
287277
Saccharomyces cerevisiae
hexanal + NADPH
-
287277
Saccharomyces cerevisiae
r
hexan-1-ol + NADP+
-
287277
Rhodococcus rhodochrous
hexanal + NADPH
-
287277
Rhodococcus rhodochrous
?
hexan-1-ol + NADP+
-
287277
Pseudomonas aeruginosa
hexanal + NADPH
-
287277
Pseudomonas aeruginosa
?
hexan-1-ol + NADP+
-
287277
Pseudomonas putida
hexanal + NADPH
-
287277
Pseudomonas putida
?
hexan-1-ol + NADP+
-
287277
Acinetobacter calcoaceticus
hexanal + NADPH
-
287277
Acinetobacter calcoaceticus
?
hexan-1-ol + NADP+
-
287277
Acinetobacter calcoaceticus
hexanal + NADPH
-
287277
Acinetobacter calcoaceticus
r
hexan-1-ol + NADP+
-
287277
Mycobacterium tuberculosis
hexanal + NADPH
-
287277
Mycobacterium tuberculosis
?
hexan-1-ol + NADP+
-
287277
Rhodotorula graminis
hexanal + NADPH
-
287277
Rhodotorula graminis
?
hexan-1-ol + NADP+
-
287277
Streptomyces rimosus
hexanal + NADPH
-
287277
Streptomyces rimosus
?
hexan-1-ol + NADP+
-
287277
Rhodococcus rhodochrous NCIMB 13259
hexanal + NADPH
-
287277
Rhodococcus rhodochrous NCIMB 13259
?
hexan-1-ol + NADP+
-
287277
Pseudomonas putida NCIMB 9494
hexanal + NADPH
-
287277
Pseudomonas putida NCIMB 9494
?
hexan-1-ol + NADP+
-
287277
Streptomyces rimosus 4018
hexanal + NADPH
-
287277
Streptomyces rimosus 4018
?
hexan-1-ol + NADP+
-
287277
Rhodotorula graminis KGX 39
hexanal + NADPH
-
287277
Rhodotorula graminis KGX 39
?
hexan-1-ol + NADP+
-
287277
Saccharomyces cerevisiae D273-10B
hexanal + NADPH
-
287277
Saccharomyces cerevisiae D273-10B
r
hexan-1-ol + NADP+
-
287277
Bacillus subtilis NCIMB 3610
hexanal + NADPH
-
287277
Bacillus subtilis NCIMB 3610
?
hexan-1-ol + NADP+
-
287277
Escherichia coli ML30
hexanal + NADPH
-
287277
Escherichia coli ML30
?
mannitol + NADP+
6.5% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
D-mannose + NADPH
-
287277
Acinetobacter calcoaceticus
r
octan-1-ol + NADP+
decrease in enzyme activity with increase in chain length
287277
Acinetobacter calcoaceticus
octanal + NADPH
-
287277
Acinetobacter calcoaceticus
r
pentan-1-ol + NADP+
-
287277
Saccharomyces cerevisiae
pentanal + NADPH
-
-
-
-
pentan-1-ol + NADP+
4% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
pentanal + NADPH
-
287277
Acinetobacter calcoaceticus
r
pentan-1-ol + NADP+
-
287277
Saccharomyces cerevisiae D273-10B
pentanal + NADPH
-
-
-
-
propan-1-ol + NADP+
28% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
propanal + NADPH
-
287277
Acinetobacter calcoaceticus
r
ribitol + NADP+
4% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
D-ribose + NADPH
-
287277
Acinetobacter calcoaceticus
r
Subunits
Subunits
Commentary
Organism
monomer
1 * 43500, SDS-PAGE
Saccharomyces cerevisiae
tetramer
4 * 40300, SDS-PAGE
Acinetobacter calcoaceticus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
13.9
-
ethanol
-
Acinetobacter calcoaceticus
19.6
-
Butanal
-
Saccharomyces cerevisiae
25.4
-
octan-1-ol
-
Acinetobacter calcoaceticus
26.3
-
Butanal
-
Acinetobacter calcoaceticus
35.1
-
ethanol
-
Saccharomyces cerevisiae
38.3
-
butan-1-ol
-
Acinetobacter calcoaceticus
38.9
-
heptan-1-ol
-
Acinetobacter calcoaceticus
39.4
-
hexan-1-ol
-
Acinetobacter calcoaceticus
39.8
-
octan-1-ol
-
Saccharomyces cerevisiae
40.3
-
hexan-1-ol
-
Saccharomyces cerevisiae
44.7
-
pentan-1-ol
-
Acinetobacter calcoaceticus
50.6
-
butan-1-ol
-
Saccharomyces cerevisiae
62.9
-
pentan-1-ol
-
Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
hexanal reduction
Acinetobacter calcoaceticus
6.8
-
hexanal reduction
Saccharomyces cerevisiae
10.2
-
hexan-1-ol oxidation
Acinetobacter calcoaceticus
10.7
-
hexan-1-ol oxidation
Saccharomyces cerevisiae
pH Range
pH Minimum
pH Maximum
Commentary
Organism
6.4
7.15
hexanal reduction, 95% of maximal rate
Acinetobacter calcoaceticus
6.4
7.15
hexanal reduction, 95% of maximal rate
Saccharomyces cerevisiae
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Acinetobacter calcoaceticus
NADPH
-
Bacillus subtilis
NADPH
-
Escherichia coli
NADPH
-
Mycobacterium tuberculosis
NADPH
-
Pseudomonas aeruginosa
NADPH
-
Pseudomonas putida
NADPH
-
Rhodococcus rhodochrous
NADPH
-
Rhodotorula graminis
NADPH
-
Saccharomyces cerevisiae
NADPH
-
Streptomyces rimosus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Acinetobacter calcoaceticus
NADPH
-
Bacillus subtilis
NADPH
-
Escherichia coli
NADPH
-
Mycobacterium tuberculosis
NADPH
-
Pseudomonas aeruginosa
NADPH
-
Pseudomonas putida
NADPH
-
Rhodococcus rhodochrous
NADPH
-
Rhodotorula graminis
NADPH
-
Saccharomyces cerevisiae
NADPH
-
Streptomyces rimosus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
4-chloromercuribenzoate
0.01 mM, complete inhibition
Acinetobacter calcoaceticus
4-chloromercuribenzoate
0.01 mM, complete inhibition; some protection of partial inhibition resulting from 0.001 mM 4-chloromercuribenzoate in the presence of 1 mM NADP+, almost complete protection with 10 mM hexan-1-ol
Saccharomyces cerevisiae
EDTA
10 mM, 20% inhibition
Saccharomyces cerevisiae
iodoacetamide
1 mM, complete inhibition
Saccharomyces cerevisiae
N-ethylmaleimide
1 mM, complete inhibition
Saccharomyces cerevisiae
Sodium azide
10 mM, 40% inhibition
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.00079
-
NADPH
-
Saccharomyces cerevisiae
0.00433
-
NADPH
-
Acinetobacter calcoaceticus
0.0202
-
NADP+
-
Saccharomyces cerevisiae
0.038
-
NADP+
-
Acinetobacter calcoaceticus
0.75
-
Butanal
-
Saccharomyces cerevisiae
1.09
-
heptan-1-ol
-
Acinetobacter calcoaceticus
1.22
-
hexan-1-ol
-
Acinetobacter calcoaceticus
3.97
-
Butanal
-
Acinetobacter calcoaceticus
5.8
-
pentan-1-ol
-
Acinetobacter calcoaceticus
6.2
-
octan-1-ol
-
Saccharomyces cerevisiae
6.8
-
hexan-1-ol
-
Saccharomyces cerevisiae
14.1
-
pentan-1-ol
-
Saccharomyces cerevisiae
52.7
-
butan-1-ol
-
Acinetobacter calcoaceticus
78.6
-
butan-1-ol
-
Saccharomyces cerevisiae
150
-
benzyl alcohol
-
Acinetobacter calcoaceticus
727
-
ethanol
-
Acinetobacter calcoaceticus
4790
-
ethanol
-
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40300
-
4 * 40300, SDS-PAGE
Acinetobacter calcoaceticus
43500
-
1 * 43500, SDS-PAGE
Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
Commentary
Organism
DEAE-Sephacel, ammonium sulfate, Sephacryl S300HR, Phenyl-Sepharose, Red A
Saccharomyces cerevisiae
DEAE-Sephacel, CM-cellulose, Sephacryl S300HR, Phenyl-Sepharose, Blue A/DEAE-Sephacel
Acinetobacter calcoaceticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
allyl alcohol + NADP+
28% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
acrylaldehyde + NADPH
-
287277
Acinetobacter calcoaceticus
r
benzyl alcohol + NADP+
-
287277
Acinetobacter calcoaceticus
benzaldehyde + NADPH
-
287277
Acinetobacter calcoaceticus
r
butan-1,4-diol + NADP+
29% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
butandialdehyde + NADPH
-
287277
Acinetobacter calcoaceticus
r
butan-1-ol + NADP+
36.3% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
butanal + NADPH
-
287277
Acinetobacter calcoaceticus
r
cis-hex-3-en-1-ol + NADP+
18.7% of activity with hexan-1ol
287277
Acinetobacter calcoaceticus
cis-hex-3-en-1-al + NADPH
-
287277
Acinetobacter calcoaceticus
r
decan-1-ol + NADP+
decrease in enzyme activity with increase in chain length
287277
Acinetobacter calcoaceticus
decanal + NADPH
-
287277
Acinetobacter calcoaceticus
r
dodecan-1-ol + NADP+
decrease in enzyme activity with increase in chain length
287277
Acinetobacter calcoaceticus
dodecanal + NADPH
-
287277
Acinetobacter calcoaceticus
r
ethanediol + NADP+
8.5% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
glyoxal + NADPH
-
287277
Acinetobacter calcoaceticus
r
ethanol + NADP+
8.5% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
ethanal + NADPH + H+
-
287277
Acinetobacter calcoaceticus
r
heptan-1-ol + NADP+
-
287277
Acinetobacter calcoaceticus
heptanal + NADPH
-
287277
Acinetobacter calcoaceticus
r
hexan-1-ol + NADP+
-
287277
Bacillus subtilis
hexanal + NADPH
-
287277
Bacillus subtilis
?
hexan-1-ol + NADP+
-
287277
Escherichia coli
hexanal + NADPH
-
287277
Escherichia coli
?
hexan-1-ol + NADP+
-
287277
Saccharomyces cerevisiae
hexanal + NADPH
-
287277
Saccharomyces cerevisiae
r
hexan-1-ol + NADP+
-
287277
Rhodococcus rhodochrous
hexanal + NADPH
-
287277
Rhodococcus rhodochrous
?
hexan-1-ol + NADP+
-
287277
Pseudomonas aeruginosa
hexanal + NADPH
-
287277
Pseudomonas aeruginosa
?
hexan-1-ol + NADP+
-
287277
Pseudomonas putida
hexanal + NADPH
-
287277
Pseudomonas putida
?
hexan-1-ol + NADP+
-
287277
Acinetobacter calcoaceticus
hexanal + NADPH
-
287277
Acinetobacter calcoaceticus
?
hexan-1-ol + NADP+
-
287277
Acinetobacter calcoaceticus
hexanal + NADPH
-
287277
Acinetobacter calcoaceticus
r
hexan-1-ol + NADP+
-
287277
Mycobacterium tuberculosis
hexanal + NADPH
-
287277
Mycobacterium tuberculosis
?
hexan-1-ol + NADP+
-
287277
Rhodotorula graminis
hexanal + NADPH
-
287277
Rhodotorula graminis
?
hexan-1-ol + NADP+
-
287277
Streptomyces rimosus
hexanal + NADPH
-
287277
Streptomyces rimosus
?
hexan-1-ol + NADP+
-
287277
Rhodococcus rhodochrous NCIMB 13259
hexanal + NADPH
-
287277
Rhodococcus rhodochrous NCIMB 13259
?
hexan-1-ol + NADP+
-
287277
Pseudomonas putida NCIMB 9494
hexanal + NADPH
-
287277
Pseudomonas putida NCIMB 9494
?
hexan-1-ol + NADP+
-
287277
Streptomyces rimosus 4018
hexanal + NADPH
-
287277
Streptomyces rimosus 4018
?
hexan-1-ol + NADP+
-
287277
Rhodotorula graminis KGX 39
hexanal + NADPH
-
287277
Rhodotorula graminis KGX 39
?
hexan-1-ol + NADP+
-
287277
Saccharomyces cerevisiae D273-10B
hexanal + NADPH
-
287277
Saccharomyces cerevisiae D273-10B
r
hexan-1-ol + NADP+
-
287277
Bacillus subtilis NCIMB 3610
hexanal + NADPH
-
287277
Bacillus subtilis NCIMB 3610
?
hexan-1-ol + NADP+
-
287277
Escherichia coli ML30
hexanal + NADPH
-
287277
Escherichia coli ML30
?
mannitol + NADP+
6.5% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
D-mannose + NADPH
-
287277
Acinetobacter calcoaceticus
r
octan-1-ol + NADP+
decrease in enzyme activity with increase in chain length
287277
Acinetobacter calcoaceticus
octanal + NADPH
-
287277
Acinetobacter calcoaceticus
r
pentan-1-ol + NADP+
-
287277
Saccharomyces cerevisiae
pentanal + NADPH
-
-
-
-
pentan-1-ol + NADP+
4% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
pentanal + NADPH
-
287277
Acinetobacter calcoaceticus
r
pentan-1-ol + NADP+
-
287277
Saccharomyces cerevisiae D273-10B
pentanal + NADPH
-
-
-
-
propan-1-ol + NADP+
28% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
propanal + NADPH
-
287277
Acinetobacter calcoaceticus
r
ribitol + NADP+
4% of activity with hexan-1-ol
287277
Acinetobacter calcoaceticus
D-ribose + NADPH
-
287277
Acinetobacter calcoaceticus
r
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 43500, SDS-PAGE
Saccharomyces cerevisiae
tetramer
4 * 40300, SDS-PAGE
Acinetobacter calcoaceticus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
13.9
-
ethanol
-
Acinetobacter calcoaceticus
19.6
-
Butanal
-
Saccharomyces cerevisiae
25.4
-
octan-1-ol
-
Acinetobacter calcoaceticus
26.3
-
Butanal
-
Acinetobacter calcoaceticus
35.1
-
ethanol
-
Saccharomyces cerevisiae
38.3
-
butan-1-ol
-
Acinetobacter calcoaceticus
38.9
-
heptan-1-ol
-
Acinetobacter calcoaceticus
39.4
-
hexan-1-ol
-
Acinetobacter calcoaceticus
39.8
-
octan-1-ol
-
Saccharomyces cerevisiae
40.3
-
hexan-1-ol
-
Saccharomyces cerevisiae
44.7
-
pentan-1-ol
-
Acinetobacter calcoaceticus
50.6
-
butan-1-ol
-
Saccharomyces cerevisiae
62.9
-
pentan-1-ol
-
Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.8
-
hexanal reduction
Acinetobacter calcoaceticus
6.8
-
hexanal reduction
Saccharomyces cerevisiae
10.2
-
hexan-1-ol oxidation
Acinetobacter calcoaceticus
10.7
-
hexan-1-ol oxidation
Saccharomyces cerevisiae
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
6.4
7.15
hexanal reduction, 95% of maximal rate
Acinetobacter calcoaceticus
6.4
7.15
hexanal reduction, 95% of maximal rate
Saccharomyces cerevisiae
Other publictions for EC 1.1.1.71
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738540
Zheng
Cofactor specificity of the bi ...
Ruminiclostridium thermocellum, Thermoanaerobacterium saccharolyticum
J. Bacteriol.
197
2610-2619
2015
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4
726699
Stekhanova
Sodium chloride-induced modula ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Biochem. Biotechnol.
171
1877-1889
2013
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6
6
728288
Liliensiek
Heterologous overexpression, p ...
Halobacterium salinarum, Halobacterium sp., Halobacterium sp. NRC-1
Mol. Biotechnol.
55
143-149
2013
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2
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1
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3
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21
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722208
Timpson
Characterization of alcohol de ...
Haloarcula marismortui
Extremophiles
16
57-66
2012
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1
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3
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1
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4
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8
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726769
Timpson
A comparison of two novel alco ...
Haloferax volcanii, Haloferax volcanii DSM 3757
Appl. Microbiol. Biotechnol.
97
195-203
2012
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1
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2
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14
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14
1
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1
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5
5
739526
Brown
Mutant alcohol dehydrogenase l ...
Ruminiclostridium thermocellum
Proc. Natl. Acad. Sci. USA
108
13752-71375
2011
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726606
Lyashenko
Expression, purification and c ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Acta Crystallogr. Sect. F
66
655-657
2010
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1
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726712
Stekhanova
Characterization of a thermost ...
Thermococcus sibiricus, Thermococcus sibiricus DSM 12597
Appl. Environ. Microbiol.
76
4096-4098
2010
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1
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14
5
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3
3
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7
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30
1
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5
2
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14
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3
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30
1
1
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5
2
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5
5
668112
Thomas
Retinoid metabolism in the rat ...
Rattus norvegicus
Br. J. Nutr.
93
59-63
2005
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1
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657019
Montesano
A novel potato defence-related ...
Solanum tuberosum
Plant Mol. Biol.
52
177-189
2003
2
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5
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15
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655563
Belyaeva
Human pancreas protein 2 (PAN2 ...
Homo sapiens
FEBS Lett.
531
489-493
2002
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1
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6
2
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14
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14
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287273
Hirschberg
N-terminal acetylation in a th ...
Gallus gallus
Cell. Mol. Life Sci.
58
1323-1326
2001
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1
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287275
Korkhin
NADP-dependent bacterial alcoh ...
Clostridium beijerinckii, Thermoanaerobacter brockii
J. Mol. Biol.
278
967-981
1998
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2
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287277
Wales
NADP-dependent alcohol dehydro ...
Acinetobacter calcoaceticus, Bacillus subtilis, Bacillus subtilis NCIMB 3610, Escherichia coli, Escherichia coli ML30, Mycobacterium tuberculosis, Pseudomonas aeruginosa, Pseudomonas putida, Pseudomonas putida NCIMB 9494, Rhodococcus rhodochrous, Rhodococcus rhodochrous NCIMB 13259, Rhodotorula graminis, Rhodotorula graminis KGX 39, Saccharomyces cerevisiae, Saccharomyces cerevisiae D273-10B, Streptomyces rimosus 4018, Streptomyces rimosus
Microbiology
140
173-183
1994
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6
17
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2
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20
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2
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35
2
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13
4
2
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10
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10
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6
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17
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35
2
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13
4
2
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287278
Brophy
A novel NADPH/NADH-dependent a ...
Moniezia expansa
FEBS Lett.
263
305-307
1990
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2
2
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287279
Hiu
Butanol-ethanol dehydrogenase ...
Clostridium beijerinckii
Appl. Environ. Microbiol.
53
697-703
1987
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287280
Hatanaka
-
Purification and properties of ...
Leuconostoc mesenteroides, Leuconostoc mesenteroides IFO 3426
Agric. Biol. Chem.
38
1819-1833
1974
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14
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287281
Rhodes
-
Co-factor specificity of plant ...
Cucumis melo
Phytochemistry
12
307-314
1973
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2
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287282
Fidge
The enzymatic reduction of ret ...
Rattus norvegicus
J. Biol. Chem.
243
4372-4379
1968
2
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14
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14
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