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Literature summary for 1.1.1.67 extracted from

  • Klimacek, M.; Nidetzky, B.
    A catalytic consensus motif for D-mannitol 2-dehydrogenase, a member of a polyol-specific long-chain dehydrogenase family, revealed by kinetic characterization of site-directed mutants of the enzyme from Pseudomonas fluorescens (2002), Biochem. J., 367, 13-18.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of wild-type enzyme and mutant enzymes in Escherichia coli Pseudomonas fluorescens

Protein Variants

Protein Variants Comment Organism
H303A mutant enzyme displays catalytic efficiency for NAD+-dependent oxidation of D-mannitol 300fold below the wild-type value Pseudomonas fluorescens
K295A mutant enzyme displays catalytic efficiency for NAD+-dependent oxidation of D-mannitol 400000fold below the wild-type value Pseudomonas fluorescens
N300A mutant enzyme displays catalytic efficiency for NAD+-dependent oxidation of D-mannitol 1000fold below the wild-type value Pseudomonas fluorescens

Organism

Organism UniProt Comment Textmining
Pseudomonas fluorescens
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose + NADH + H+ Asn300 has an auxiliary role in stabilization of the transition state of hydride transfer and His303 contributes to substrate positioning, role of Lys295 in general base enzymic catalysis Pseudomonas fluorescens D-mannitol + NAD+
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Synonyms

Synonyms Comment Organism
M2DH
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Pseudomonas fluorescens
PsM2DH
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Pseudomonas fluorescens