Cloned (Comment) | Organism |
---|---|
expression of wild-type enzyme and mutant enzymes in Escherichia coli | Pseudomonas fluorescens |
Protein Variants | Comment | Organism |
---|---|---|
H303A | mutant enzyme displays catalytic efficiency for NAD+-dependent oxidation of D-mannitol 300fold below the wild-type value | Pseudomonas fluorescens |
K295A | mutant enzyme displays catalytic efficiency for NAD+-dependent oxidation of D-mannitol 400000fold below the wild-type value | Pseudomonas fluorescens |
N300A | mutant enzyme displays catalytic efficiency for NAD+-dependent oxidation of D-mannitol 1000fold below the wild-type value | Pseudomonas fluorescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADH + H+ | Asn300 has an auxiliary role in stabilization of the transition state of hydride transfer and His303 contributes to substrate positioning, role of Lys295 in general base enzymic catalysis | Pseudomonas fluorescens | D-mannitol + NAD+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
M2DH | - |
Pseudomonas fluorescens |
PsM2DH | - |
Pseudomonas fluorescens |