Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Leuconostoc pseudomesenteroides |
General Stability | Organism |
---|---|
DTT stabilizes | Leuconostoc pseudomesenteroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | 50 mM, pH 5.4, 72% inhibition | Leuconostoc pseudomesenteroides | |
EDTA | 20 mM, complete inhibition, can be restored by 20 mM Zn2+, but not by Mn2+ or Mg2+ | Leuconostoc pseudomesenteroides | |
imidazole | 175 mM,pH 7.0, 74% inhibition | Leuconostoc pseudomesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
12 | - |
D-mannitol | pH 8.6, 30°C | Leuconostoc pseudomesenteroides | |
44 | - |
D-fructose | pH 5.4, 30°C | Leuconostoc pseudomesenteroides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | cofactor | Leuconostoc pseudomesenteroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43000 | - |
4 * 43000, SDS-PAGE | Leuconostoc pseudomesenteroides |
155000 | - |
gel filtration | Leuconostoc pseudomesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc pseudomesenteroides | Q83VI5 | ATCC 12291 | - |
Purification (Comment) | Organism |
---|---|
- |
Leuconostoc pseudomesenteroides |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
450 | - |
- |
Leuconostoc pseudomesenteroides |
Storage Stability | Organism |
---|---|
4°C or 20°C, enzyme loses approximately 60% of its specific activity after storage for 3 weeks in presence of 1 mM DTT, without DTT the same loss is observed after 2 days | Leuconostoc pseudomesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose + NADH + H+ | - |
Leuconostoc pseudomesenteroides | D-mannitol + NAD+ | - |
r | |
D-glucitol + NAD+ | 4% of the activity with D-mannitol | Leuconostoc pseudomesenteroides | ? | - |
? | |
D-mannitol + NADP+ | 32% of the activity with NAD+ | Leuconostoc pseudomesenteroides | D-fructose + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 43000, SDS-PAGE | Leuconostoc pseudomesenteroides |
Synonyms | Comment | Organism |
---|---|---|
mannitol 2-dehydrogenase | - |
Leuconostoc pseudomesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | - |
reduction of D-fructose | Leuconostoc pseudomesenteroides |
8.6 | - |
oxidation of D-mannitol | Leuconostoc pseudomesenteroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Leuconostoc pseudomesenteroides | |
NADH | - |
Leuconostoc pseudomesenteroides | |
NADP+ | 32% of the activity with NAD+ | Leuconostoc pseudomesenteroides |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Leuconostoc pseudomesenteroides | chromatofocusing | - |
4.2 |