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Literature summary for 1.1.1.67 extracted from

  • Aarnikunnas, J.; Ronnholm, K.; Palva, A.
    The mannitol dehydrogenase gene (mdh) from Leuconostoc mesenteroides is distinct from other known bacterial mdh genes (2002), Appl. Microbiol. Biotechnol., 59, 665-671.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
fusion of six His codons to the 3'-end of the mdh gene and expression in Escherichia coli M15. The enzyme shares significant sequence similarity with the medium-chain dehydrogenase/reductase protein family Leuconostoc mesenteroides

Inhibitors

Inhibitors Comment Organism Structure
PCMB 0.1 mM, complete inhibition Leuconostoc mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
32
-
D-mannitol pH 8.6 Leuconostoc mesenteroides
71
-
D-fructose pH 5.3 Leuconostoc mesenteroides

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
1 * 41000, SDS-PAGE Leuconostoc mesenteroides

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides Q8KQG6 ATCC-9135
-
Leuconostoc mesenteroides ATCC -9135 Q8KQG6 ATCC-9135
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Leuconostoc mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-fructose 1-phosphate + NADH
-
Leuconostoc mesenteroides ?
-
?
D-fructose 1-phosphate + NADH
-
Leuconostoc mesenteroides ATCC -9135 ?
-
?
D-mannitol + NAD+
-
Leuconostoc mesenteroides D-fructose + NADH + H+
-
r
D-mannitol + NAD+
-
Leuconostoc mesenteroides ATCC -9135 D-fructose + NADH + H+
-
r

Subunits

Subunits Comment Organism
monomer 1 * 41000, SDS-PAGE Leuconostoc mesenteroides

Synonyms

Synonyms Comment Organism
MDH
-
Leuconostoc mesenteroides

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Leuconostoc mesenteroides
NADH
-
Leuconostoc mesenteroides