Cloned (Comment) | Organism |
---|---|
fusion of six His codons to the 3'-end of the mdh gene and expression in Escherichia coli M15. The enzyme shares significant sequence similarity with the medium-chain dehydrogenase/reductase protein family | Leuconostoc mesenteroides |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PCMB | 0.1 mM, complete inhibition | Leuconostoc mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
32 | - |
D-mannitol | pH 8.6 | Leuconostoc mesenteroides | |
71 | - |
D-fructose | pH 5.3 | Leuconostoc mesenteroides |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
1 * 41000, SDS-PAGE | Leuconostoc mesenteroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides | Q8KQG6 | ATCC-9135 | - |
Leuconostoc mesenteroides ATCC -9135 | Q8KQG6 | ATCC-9135 | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Leuconostoc mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-fructose 1-phosphate + NADH | - |
Leuconostoc mesenteroides | ? | - |
? | |
D-fructose 1-phosphate + NADH | - |
Leuconostoc mesenteroides ATCC -9135 | ? | - |
? | |
D-mannitol + NAD+ | - |
Leuconostoc mesenteroides | D-fructose + NADH + H+ | - |
r | |
D-mannitol + NAD+ | - |
Leuconostoc mesenteroides ATCC -9135 | D-fructose + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, SDS-PAGE | Leuconostoc mesenteroides |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Leuconostoc mesenteroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Leuconostoc mesenteroides | |
NADH | - |
Leuconostoc mesenteroides |