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show all sequences of 1.1.1.65

Purification, molecular cloning, and catalytic activity of Schizosaccharomyces pombe pyridoxal reductase

Nakano, M.; Morita, T.; Yamamoto, T.; Sano, H.; Ashiuchi, M.; Masui, R.; Kuramitsu, S.; Toshiharu, Y.; J. Biol. Chem. 274, 23185-23190 (1999)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Schizosaccharomyces pombe
General Stability
General Stability
Organism
purified enzyme very unstable but can be stabilized by low concentrations of various detergents such as Tween 40
Schizosaccharomyces pombe
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.096
-
2-nitrobenzaldehyde
-
Schizosaccharomyces pombe
0.9
-
pyridoxal
-
Schizosaccharomyces pombe
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
gel filtration
Schizosaccharomyces pombe
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pyridoxine + NADP+
Schizosaccharomyces pombe
-
pyridoxal + NADPH
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Schizosaccharomyces pombe
O14295
-
-
Purification (Commentary)
Commentary
Organism
-
Schizosaccharomyces pombe
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-nitrobenzaldehyde + NADPH
-
287236
Schizosaccharomyces pombe
2-nitrobenzyl alcohol + NADP+
-
-
-
?
pyridoxine + NADP+
-
287236
Schizosaccharomyces pombe
pyridoxal + NADPH
-
-
-
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.5
for formation of pyridoxine
Schizosaccharomyces pombe
7.5
8.5
for formation of pyridoxal
Schizosaccharomyces pombe
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Schizosaccharomyces pombe
NADPH
-
Schizosaccharomyces pombe
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Schizosaccharomyces pombe
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Schizosaccharomyces pombe
NADPH
-
Schizosaccharomyces pombe
General Stability (protein specific)
General Stability
Organism
purified enzyme very unstable but can be stabilized by low concentrations of various detergents such as Tween 40
Schizosaccharomyces pombe
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.096
-
2-nitrobenzaldehyde
-
Schizosaccharomyces pombe
0.9
-
pyridoxal
-
Schizosaccharomyces pombe
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41000
-
gel filtration
Schizosaccharomyces pombe
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
pyridoxine + NADP+
Schizosaccharomyces pombe
-
pyridoxal + NADPH
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Schizosaccharomyces pombe
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-nitrobenzaldehyde + NADPH
-
287236
Schizosaccharomyces pombe
2-nitrobenzyl alcohol + NADP+
-
-
-
?
pyridoxine + NADP+
-
287236
Schizosaccharomyces pombe
pyridoxal + NADPH
-
-
-
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.5
7.5
for formation of pyridoxine
Schizosaccharomyces pombe
7.5
8.5
for formation of pyridoxal
Schizosaccharomyces pombe
Other publictions for EC 1.1.1.65
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
287236
Nakano
Purification, molecular clonin ...
Schizosaccharomyces pombe
J. Biol. Chem.
274
23185-23190
1999
-
-
1
-
-
1
-
2
-
-
1
1
-
6
-
-
1
-
-
-
-
-
2
-
-
-
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
1
-
-
-
2
-
-
1
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
287235
Holzer
Purification and characterizat ...
Saccharomyces cerevisiae
Biochim. Biophys. Acta
48
71-76
1961
-
-
-
-
-
-
6
4
-
1
-
1
-
2
-
-
1
-
-
-
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
6
-
4
-
1
-
1
-
-
-
1
-
-
-
-
3
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-