BRENDA - Enzyme Database show
show all sequences of 1.1.1.64

Amino acid substitution of arginine 80 in 17beta-hydroxysteroid dehydrogenase type 3 and its effect on NADPH cofactor binding and oxidation/reduction kinetics

McKeever, B.M.; Hawkins, B.K.; Geissler, W.M.; Wu, L.; Sheridan, R.P.; Mosley, R.T.; Andersson, S.; Biochim. Biophys. Acta 1601, 29-37 (2002)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
R80I
mutant enzyme shows negligible conversion of androst-4-ene-3,17-dione to testosterone. The Km-value for androstenedione is 80fold higher than the KM-value for the wild-type enzyme
Homo sapiens
R80K
conversion of androst-4-ene-3,17-dione to testosterone is reduced as compared to wild-type enzyme. The Km-value for androstenedione is 3.5fold higher than the KM-value for the wild-type enzyme
Homo sapiens
R80L
conversion of androst-4-ene-3,17-dione to testosterone is strongly reduced as compared to wild-type enzyme. The Km-value for androstenedione is 21.6fold higher than the KM-value for the wild-type enzyme
Homo sapiens
R80M
mutant enzyme shows negligible conversion of androst-4-ene-3,17-dione to testosterone. The Km-value for androstenedione is 90.7fold higher than the KM-value for the wild-type enzyme
Homo sapiens
R80Q
mutant enzyme shows negligible conversion of androst-4-ene-3,17-dione to testosterone. The Km-value for androstenedione is 61.5fold higher than the KM-value for the wild-type enzyme
Homo sapiens
R80Y
mutant enzyme shows negligible conversion of androst-4-ene-3,17-dione to testosterone. The Km-value for androstenedione is 85.1fold higher than the KM-value for the wild-type enzyme
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
the KM-values for R80 mutant enzymes are higher than the Km-value of the wild-type enzyme: for R80K 3.5fold, for R80L 21.6fold, for R80M 90.7fold, for R80Q 61.5fold, for R80Y 61.5fold, for R80Y 85.1fold, for R80I 80fold
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
androst-4-ene-3,17-dione + NADPH
Homo sapiens
the enzyme plays a central role in the development of the male phenotype. Mutations that inactivate the enzyme give rise to a rare form of male pseudohermaphroditism, referred to as 17beta-HSD-3 deficiency
testosterone + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
HEK-293 cell
-
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
androst-4-ene-3,17-dione + NADPH
-
658172
Homo sapiens
testosterone + NADP+
-
-
-
?
androst-4-ene-3,17-dione + NADPH
the enzyme plays a central role in the development of the male phenotype. Mutations that inactivate the enzyme give rise to a rare form of male pseudohermaphroditism, referred to as 17beta-HSD-3 deficiency
658172
Homo sapiens
testosterone + NADP+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
no activity with NADH
Homo sapiens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
no activity with NADH
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
R80I
mutant enzyme shows negligible conversion of androst-4-ene-3,17-dione to testosterone. The Km-value for androstenedione is 80fold higher than the KM-value for the wild-type enzyme
Homo sapiens
R80K
conversion of androst-4-ene-3,17-dione to testosterone is reduced as compared to wild-type enzyme. The Km-value for androstenedione is 3.5fold higher than the KM-value for the wild-type enzyme
Homo sapiens
R80L
conversion of androst-4-ene-3,17-dione to testosterone is strongly reduced as compared to wild-type enzyme. The Km-value for androstenedione is 21.6fold higher than the KM-value for the wild-type enzyme
Homo sapiens
R80M
mutant enzyme shows negligible conversion of androst-4-ene-3,17-dione to testosterone. The Km-value for androstenedione is 90.7fold higher than the KM-value for the wild-type enzyme
Homo sapiens
R80Q
mutant enzyme shows negligible conversion of androst-4-ene-3,17-dione to testosterone. The Km-value for androstenedione is 61.5fold higher than the KM-value for the wild-type enzyme
Homo sapiens
R80Y
mutant enzyme shows negligible conversion of androst-4-ene-3,17-dione to testosterone. The Km-value for androstenedione is 85.1fold higher than the KM-value for the wild-type enzyme
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
the KM-values for R80 mutant enzymes are higher than the Km-value of the wild-type enzyme: for R80K 3.5fold, for R80L 21.6fold, for R80M 90.7fold, for R80Q 61.5fold, for R80Y 61.5fold, for R80Y 85.1fold, for R80I 80fold
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
androst-4-ene-3,17-dione + NADPH
Homo sapiens
the enzyme plays a central role in the development of the male phenotype. Mutations that inactivate the enzyme give rise to a rare form of male pseudohermaphroditism, referred to as 17beta-HSD-3 deficiency
testosterone + NADP+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
HEK-293 cell
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
androst-4-ene-3,17-dione + NADPH
-
658172
Homo sapiens
testosterone + NADP+
-
-
-
?
androst-4-ene-3,17-dione + NADPH
the enzyme plays a central role in the development of the male phenotype. Mutations that inactivate the enzyme give rise to a rare form of male pseudohermaphroditism, referred to as 17beta-HSD-3 deficiency
658172
Homo sapiens
testosterone + NADP+
-
-
-
?
Other publictions for EC 1.1.1.64
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724719
Matsunaga
Pathophysiological roles of al ...
Homo sapiens
Chem. Biol. Interact.
202
234-242
2013
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1
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1
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1
1
1
1
1
1
724850
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The microsomal enzyme 17beta-h ...
Homo sapiens
Endocrinology
154
205-213
2013
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1
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1
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724915
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Homo sapiens
Eur. J. Med. Chem.
62
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724916
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Homo sapiens
Eur. J. Med. Chem.
62
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2013
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1
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4
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723866
Jackson
Structure of AKR1C3 with 3-phe ...
Homo sapiens
Acta Crystallogr. Sect. F
68
409-413
2012
-
-
1
1
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1
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1
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724533
Chen
Crystal structures of AKR1C3 c ...
Homo sapiens
Bioorg. Med. Chem. Lett.
22
3492-3497
2012
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724536
Harada
Identification of oxazolidined ...
Homo sapiens
Bioorg. Med. Chem. Lett.
22
504-507
2012
-
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2
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724538
Sinreih
N-Benzoyl anthranilic acid der ...
Homo sapiens
Bioorg. Med. Chem. Lett.
22
5948-5951
2012
-
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4
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724555
Harada
Discovery of potent and orally ...
Homo sapiens, Rattus norvegicus
Bioorg. Med. Chem.
20
3242-3254
2012
-
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1
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6
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724715
Yuan
Effects of phthalates on 3beta ...
Homo sapiens, Rattus norvegicus
Chem. Biol. Interact.
195
180-188
2012
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4
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8
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2
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725153
Miller
Aldo-keto reductase family 1 m ...
Homo sapiens
Int. J. Clin. Exp. Pathol.
5
278-289
2012
-
1
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1
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8
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1
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8
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725671
Brozic
Selective inhibitors of aldo-k ...
Homo sapiens
J. Med. Chem.
55
7417-7424
2012
-
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725825
Byrns
Overexpression of aldo-keto re ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
130
7-15
2012
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1
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724517
Adeniji
Discovery of substituted 3-(ph ...
Homo sapiens
Bioorg. Med. Chem. Lett.
21
1464-1468
2011
-
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3
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724550
Maltais
Development of 3-substituted-a ...
Homo sapiens
Bioorg. Med. Chem.
19
4652-4668
2011
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726493
Latif
Role of 11beta-OH-C(19) and C( ...
Rattus norvegicus
Steroids
76
682-689
2011
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725152
Zakharov
Suppressed expression of type ...
Homo sapiens
Int. J. Clin. Exp. Pathol.
3
608-617
2010
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1
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1
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699845
Hu
The (+)- and (-)-gossypols pot ...
Homo sapiens, Rattus norvegicus
J. Steroid Biochem. Mol. Biol.
115
14-19
2009
-
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4
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2
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4
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4
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2
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2
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4
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700187
Day
Development of hormone-depende ...
Homo sapiens
Mol. Cell. Endocrinol.
301
251-258
2009
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1
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3
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1
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1
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1
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1
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3
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1
1
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3
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1
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1
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712571
Nakamura
Type 5 17-hydroxysteroid dehy ...
Homo sapiens
J. Clin. Endocrinol. Metab.
94
2192-2198
2009
-
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1
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11
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Androgen metabolism via 17beta ...
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6
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14
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657673
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Human 17beta-hydroxysteroid de ...
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2
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2
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1
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Canis lupus familiaris, Homo sapiens, Rattus norvegicus
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6
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21
32
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658172
McKeever
Amino acid substitution of arg ...
Homo sapiens
Biochim. Biophys. Acta
1601
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2002
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6
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2
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1
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6
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1
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1
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2
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659587
le Lain
Some coumarins and triphenylet ...
Homo sapiens
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6
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1
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6
6
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287221
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Molecular genetics and pathoph ...
Homo sapiens
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287222
Baker
Unusual evolution of 11beta- a ...
Homo sapiens
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1995
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287223
Geissler
Male pseudohermaphroditism cau ...
Homo sapiens
Nature Genet.
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1994
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Hara
Kinetic and stereochemical stu ...
Mus musculus
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287225
Inano
Testicular 17beta-hydroxystero ...
Oryctolagus cuniculus, Ovis aries, Rattus norvegicus, Sus scrofa
Steroids
48
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1986
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287226
Hara
Guinea-pig liver testosterone ...
Cavia porcellus
Biochem. J.
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1985
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10
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287227
Inano
Amino acid composition and imm ...
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1980
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287228
Kageura
Purification and properties of ...
Cavia porcellus
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163
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1977
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287229
Inano
Relationship between steroids ...
Sus scrofa
Eur. J. Biochem.
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Testicular 17beta-hydroxystero ...
Rattus norvegicus, Sus scrofa
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6
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1975
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287231
Inano
Purificatition and properties ...
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287232
Oshima
On testicular 17beta-hydroxyst ...
Homo sapiens, Oryctolagus cuniculus
Biochim. Biophys. Acta
306
227-236
1973
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Villee
Some properties of the pyridin ...
Cavia porcellus
J. Biol. Chem.
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287234
Endahl
Separation of a triphosphopyri ...
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