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Literature summary for 1.1.1.62 extracted from
- New insights into human 17beta-hydroxysteroid dehydrogenase type 14: first crystal structures in complex with a steroidal ligand and with a potent nonsteroidal inhibitor (2016), J. Med. Chem., 59, 6961-6967.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of 17beta-HSD14 wild-type variants S205 and T205 in Escherichia coli strain BL21 pLysS | Homo sapiens |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| the purified enzyme variants T205 and S205 in apoform, as holoenzyme, or in complex with estrone or inhibitor [6-(3,4-dihydroxyphenyl)pyridin-2-yl]-(4-fluoro-3-hydroxyphenyl)methanone, ternary complex enzyme-cofactor-inhibitor, X-ray diffraction structure determination and analysis at 1.52-2.02 A resolution | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S205T | natural genetic polymorphism | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| [6-(3,4-dihydroxyphenyl)pyridin-2-yl]-(4-fluoro-3-hydroxyphenyl)methanone | a potent nonsteroidal inhibitor, binding structure, overview | Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
| 0.00062 | - |
17beta-estradiol | 17beta-HSD14 variant S205, pH and temperature not specified in the publication | Homo sapiens | |
| 0.00079 | - |
17beta-estradiol | 17beta-HSD14 variant T205, pH and temperature not specified in the publication | Homo sapiens | |
| 0.0066 | - |
5-androstene-3beta,17beta-diol | 17beta-HSD14 variant S205, pH and temperature not specified in the publication | Homo sapiens | |
| 0.0078 | - |
5-androstene-3beta,17beta-diol | 17beta-HSD14 variant T205, pH and temperature not specified in the publication | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Homo sapiens | 5829 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17beta-estradiol + NAD+ | Homo sapiens | - |
estrone + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | Q9BPX1 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | - |
Homo sapiens | - |
| brain | - |
Homo sapiens | - |
| breast cancer cell | - |
Homo sapiens | - |
| esophagus | - |
Homo sapiens | - |
| eye | - |
Homo sapiens | - |
| heart | - |
Homo sapiens | - |
| kidney | - |
Homo sapiens | - |
| liver | - |
Homo sapiens | - |
| placenta | - |
Homo sapiens | - |
| rectum | - |
Homo sapiens | - |
| salivary gland | - |
Homo sapiens | - |
| skeletal muscle | - |
Homo sapiens | - |
| testis | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 17beta-estradiol + NAD+ | - |
Homo sapiens | estrone + NADH + H+ | - |
r | |
| 5-androstene-3beta,17beta-diol + NAD+ | - |
Homo sapiens | ? + NADH + H+ | - |
? | |
| testosterone + NAD+ | - |
Homo sapiens | androst-4-ene-3,17-dione + NADH + H+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 17beta-HSD14 | - |
Homo sapiens |
| 17beta-hydroxysteroid dehydrogenase type 14 | - |
Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00028 | - |
5-androstene-3beta,17beta-diol | 17beta-HSD14 variant S205, pH and temperature not specified in the publication | Homo sapiens | |
| 0.00032 | - |
5-androstene-3beta,17beta-diol | 17beta-HSD14 variant T205, pH and temperature not specified in the publication | Homo sapiens | |
| 0.0004 | - |
17beta-estradiol | 17beta-HSD14 variant S205, pH and temperature not specified in the publication | Homo sapiens | |
| 0.00055 | - |
17beta-estradiol | 17beta-HSD14 variant T205, pH and temperature not specified in the publication | Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Homo sapiens | |
| NADH | - |
Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.000007 | - |
[6-(3,4-dihydroxyphenyl)pyridin-2-yl]-(4-fluoro-3-hydroxyphenyl)methanone | pH and temperature not specified in the publication | Homo sapiens | |
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