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Literature summary for 1.1.1.62 extracted from

  • Ohno, S.; Nishikawa, K.; Honda, Y.; Nakajin, S.
    Expression in E. coli and tissue distribution of the human homologue of the mouse Ke 6 gene, 17beta-hydroxysteroid dehydrogenase type 8 (2008), Mol. Cell. Biochem., 309, 209-215.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli as an enzymatically active His-tag fusion protein Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0464
-
testosterone 37°C, pH 7.4 Homo sapiens
0.118
-
estradiol 37°C, pH 7.4 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
x * 32000, SDS-PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain
-
Homo sapiens
-
cerebellum
-
Homo sapiens
-
heart
-
Homo sapiens
-
kidney
-
Homo sapiens
-
kidney high expression Homo sapiens
-
liver
-
Homo sapiens
-
lung
-
Homo sapiens
-
ovary
-
Homo sapiens
-
placenta
-
Homo sapiens
-
placenta high expression Homo sapiens
-
prostate
-
Homo sapiens
-
prostate high expression Homo sapiens
-
small intestine
-
Homo sapiens
-
stomach
-
Homo sapiens
-
testis
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5-androstene-3beta,17beta-diol + NAD+ 5.9% of the activity with estradiol Homo sapiens 5-androstene-3-beta-ol-17-one + NADH
-
?
5alpha-dihydrotestosterone + NAD+ 3.2% of the activity with estradiol Homo sapiens 5alpha-androstan-3,17-dione + NADH + H+
-
?
estradiol-17beta + NAD+ enzyme catalyzes the reduction of estrone to estradiol, the rate is 2.3% of the activity with estradiol Homo sapiens estrone + NADH + H+
-
?
testosterone + NAD+ 2.4% of the activity with estradiol Homo sapiens 4-androstene-3,17-dione + NADH + H+
-
?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
17beta-HSD type 8
-
Homo sapiens
17beta-hydroxysteroid dehydrogenase type 8
-
Homo sapiens