BRENDA - Enzyme Database show
show all sequences of 1.1.1.61

Production of 4-hydroxybutyrate from succinate semialdehyde in butyrate biosynthesis in Porphyromonas gingivalis

Yoshida, Y.; Sato, M.; Nagano, K.; Hasegawa, Y.; Okamoto, T.; Yoshimura, F.; Biochim. Biophys. Acta 1850, 2582-2591 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21 cells
Porphyromonas gingivalis
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
succinate semialdehyde
with NADH as cosubstrate, at pH 8.0 and 37°C
Porphyromonas gingivalis
0.41
-
NADH
with NADH as cosubstrate, at pH 8.0 and 37°C
Porphyromonas gingivalis
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mn2+
most favorable metal ion for enzymatic activity
Porphyromonas gingivalis
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
gel filtration
Porphyromonas gingivalis
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinate semialdehyde + NADH + H+
Porphyromonas gingivalis
-
4-hydroxybutanoate + NAD+
-
-
?
succinate semialdehyde + NADH + H+
Porphyromonas gingivalis ATCC 33277
-
4-hydroxybutanoate + NAD+
-
-
?
succinate semialdehyde + NADPH + H+
Porphyromonas gingivalis
-
4-hydroxybutanoate + NADP+
-
-
?
succinate semialdehyde + NADPH + H+
Porphyromonas gingivalis ATCC 33277
-
4-hydroxybutanoate + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Porphyromonas gingivalis
-
-
-
Porphyromonas gingivalis ATCC 33277
-
-
-
Purification (Commentary)
Commentary
Organism
glutathione Sepharose column chromatography
Porphyromonas gingivalis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
succinate semialdehyde + NADH + H+
-
737764
Porphyromonas gingivalis
4-hydroxybutanoate + NAD+
-
-
-
?
succinate semialdehyde + NADH + H+
-
737764
Porphyromonas gingivalis ATCC 33277
4-hydroxybutanoate + NAD+
-
-
-
?
succinate semialdehyde + NADPH + H+
-
737764
Porphyromonas gingivalis
4-hydroxybutanoate + NADP+
-
-
-
?
succinate semialdehyde + NADPH + H+
-
737764
Porphyromonas gingivalis ATCC 33277
4-hydroxybutanoate + NADP+
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 37000, SDS-PAGE
Porphyromonas gingivalis
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1329
-
succinate semialdehyde
with NADH as cosubstrate, at pH 8.0 and 37°C
Porphyromonas gingivalis
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Porphyromonas gingivalis
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9
more than 80% activity between pH 7.0 and 9.0
Porphyromonas gingivalis
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
NADH is a more favorable cofactor for the enzyme than NADPH
Porphyromonas gingivalis
NADPH
NADH is a more favorable cofactor for the enzyme than NADPH
Porphyromonas gingivalis
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 cells
Porphyromonas gingivalis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
NADH is a more favorable cofactor for the enzyme than NADPH
Porphyromonas gingivalis
NADPH
NADH is a more favorable cofactor for the enzyme than NADPH
Porphyromonas gingivalis
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.15
-
succinate semialdehyde
with NADH as cosubstrate, at pH 8.0 and 37°C
Porphyromonas gingivalis
0.41
-
NADH
with NADH as cosubstrate, at pH 8.0 and 37°C
Porphyromonas gingivalis
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mn2+
most favorable metal ion for enzymatic activity
Porphyromonas gingivalis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
42000
-
gel filtration
Porphyromonas gingivalis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
succinate semialdehyde + NADH + H+
Porphyromonas gingivalis
-
4-hydroxybutanoate + NAD+
-
-
?
succinate semialdehyde + NADH + H+
Porphyromonas gingivalis ATCC 33277
-
4-hydroxybutanoate + NAD+
-
-
?
succinate semialdehyde + NADPH + H+
Porphyromonas gingivalis
-
4-hydroxybutanoate + NADP+
-
-
?
succinate semialdehyde + NADPH + H+
Porphyromonas gingivalis ATCC 33277
-
4-hydroxybutanoate + NADP+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
glutathione Sepharose column chromatography
Porphyromonas gingivalis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
succinate semialdehyde + NADH + H+
-
737764
Porphyromonas gingivalis
4-hydroxybutanoate + NAD+
-
-
-
?
succinate semialdehyde + NADH + H+
-
737764
Porphyromonas gingivalis ATCC 33277
4-hydroxybutanoate + NAD+
-
-
-
?
succinate semialdehyde + NADPH + H+
-
737764
Porphyromonas gingivalis
4-hydroxybutanoate + NADP+
-
-
-
?
succinate semialdehyde + NADPH + H+
-
737764
Porphyromonas gingivalis ATCC 33277
4-hydroxybutanoate + NADP+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 37000, SDS-PAGE
Porphyromonas gingivalis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1329
-
succinate semialdehyde
with NADH as cosubstrate, at pH 8.0 and 37°C
Porphyromonas gingivalis
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
-
Porphyromonas gingivalis
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9
more than 80% activity between pH 7.0 and 9.0
Porphyromonas gingivalis
Other publictions for EC 1.1.1.61
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737764
Yoshida
Production of 4-hydroxybutyrat ...
Porphyromonas gingivalis, Porphyromonas gingivalis ATCC 33277
Biochim. Biophys. Acta
1850
2582-2591
2015
-
-
1
-
-
-
-
2
-
1
1
4
-
10
-
-
1
-
-
-
-
-
4
1
-
-
-
1
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
2
-
1
1
4
-
-
-
1
-
-
-
-
4
1
-
-
-
1
1
1
-
-
-
-
-
-
-
-
698903
Saito
Metabolite profiling reveals Y ...
Escherichia coli, Escherichia coli K12 BW25113
J. Biol. Chem.
284
16442-16451
2009
-
-
1
-
-
-
-
5
-
1
2
2
-
4
-
-
1
-
-
-
4
-
11
1
-
-
-
-
-
-
-
2
-
-
-
-
-
1
3
-
-
-
-
-
-
8
-
1
3
2
-
-
-
1
-
-
4
-
11
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
656423
Bravo
Reliable, sensitive, rapid and ...
Cupriavidus necator
J. Forensic Sci.
49
379-387
2004
-
1
1
-
-
1
-
2
-
-
2
1
-
2
-
-
1
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
-
-
2
-
-
2
1
-
-
-
1
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
656170
Breitkreuz
A novel gamma-hydroxybutyrate ...
Arabidopsis thaliana
J. Biol. Chem.
278
41552-41556
2003
-
-
1
-
-
-
-
-
-
-
1
1
-
4
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
4
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
287171
Wolff
Purification and characterizat ...
Clostridium kluyveri
Protein Expr. Purif.
6
206-212
1995
-
-
-
-
-
-
1
4
-
2
2
-
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
2
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
287170
Nirenberg
Enzymatic utilization of gamma ...
Pseudomonas sp.
J. Biol. Chem.
235
954-960
1960
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-