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Literature summary for 1.1.1.61 extracted from

  • Saito, N.; Robert, M.; Kochi, H.; Matsuo, G.; Kakazu, Y.; Soga, T.; Tomita, M.
    Metabolite profiling reveals YihU as a novel hydroxybutyrate dehydrogenase for alternative succinic semialdehyde metabolism in Escherichia coli (2009), J. Biol. Chem., 284, 16442-16451.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a histidine-tagged recombinant protein in Escherichia coli Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1
-
3-hydroxypropane sulfonate
-
Escherichia coli
1
-
3-hydroxypropane sulfonate in 100 mM Tris-HCl buffer (pH 8.8), at 37°C Escherichia coli
4.3
-
Succinic semialdehyde
-
Escherichia coli
4.3
-
Succinic semialdehyde in 100 mM MOPS-KOH buffer (pH 7.2), at 37°C Escherichia coli
9
-
methylglyoxal
-
Escherichia coli
9
-
methylglyoxal in 100 mM Tris-HCl buffer (pH 8.8), at 37°C Escherichia coli
102
-
4-hydroxybutyrate
-
Escherichia coli
102
-
gamma-hydroxybutyrate in 100 mM Tris-HCl buffer (pH 8.8), at 37°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no significant differences in the activity are observed when using different divalent metals (Mg2+, Mn2+, Ca2+, Co2+, and Zn2+) Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31154
-
4 * 31154, calculated from amino acid sequence Escherichia coli
143000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
gamma-hydroxybutyrate + NAD+ Escherichia coli
-
succinic semialdehyde + NADH + H+
-
r
succinic semialdehyde + NADH + H+ Escherichia coli succinic semialdehyde is the preferred substrate gamma-hydroxybutyrate + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli P0A9V8
-
-
Escherichia coli K12 BW25113 P0A9V8
-
-

Purification (Commentary)

Purification (Comment) Organism
purified using cobalt-based immobilized TALON metal affinity chromatography resins with a gravity-flow column Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.062
-
substrate: gamma-hydroxybutyrate Escherichia coli
0.067
-
substrate: methylglyoxal Escherichia coli
0.2
-
substrate: succinic semialdehyde Escherichia coli
9
-
substrate: 3-hydroxypropane sulfonate Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypropane sulfonate + NAD+
-
Escherichia coli ? + NADH
-
?
3-hydroxypropane sulfonate + NAD+
-
Escherichia coli K12 BW25113 ? + NADH
-
?
3-hydroxypropane sulfonate + NAD+
-
Escherichia coli ? + NADH + H+
-
r
gamma-hydroxybutyrate + NAD+
-
Escherichia coli succinic semialdehyde + NADH + H+
-
r
methylglyoxal + NAD+
-
Escherichia coli ? + NADH
-
?
methylglyoxal + NAD+
-
Escherichia coli K12 BW25113 ? + NADH
-
?
methylglyoxal + NAD+
-
Escherichia coli ? + NADH + H+
-
r
additional information no activity with D-glycerate and 3-hydroxypropanoate Escherichia coli ?
-
?
succinic semialdehyde + NADH + H+
-
Escherichia coli 4-hydroxybutyrate + NAD+
-
r
succinic semialdehyde + NADH + H+
-
Escherichia coli K12 BW25113 4-hydroxybutyrate + NAD+
-
r
succinic semialdehyde + NADH + H+ succinic semialdehyde is the preferred substrate Escherichia coli gamma-hydroxybutyrate + NAD+
-
r

Subunits

Subunits Comment Organism
homotetramer 4 * 31154, calculated from amino acid sequence Escherichia coli

Synonyms

Synonyms Comment Organism
gamma-hydroxybutyrate dehydrogenase
-
Escherichia coli
GHBDH
-
Escherichia coli
uncharacterized oxidoreductase YihU
-
Escherichia coli
yihU
-
Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Escherichia coli
NADH
-
Escherichia coli
NADH the NADH-dependent succinic semialdehyde reductase activity is 4fold higher than that with NADPH Escherichia coli

General Information

General Information Comment Organism
malfunction a yihU deletion mutant displays reduced tolerance to the cytotoxic effects of exogenous addition of succinic semialdehyde Escherichia coli
metabolism the enzyme is involved in the metabolism of succinic semialdehyde, and other potentially toxic intermediates that may accumulate under stress conditions in Escherichia coli Escherichia coli