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show all sequences of 1.1.1.61

Reliable, sensitive, rapid and quantitative enzyme-based assay for gamma-hydroxybutyric acid (GHB)

Bravo, D.T.; Harris, D.O.; Parsons, S.M.; J. Forensic Sci. 49, 379-387 (2004)

Data extracted from this reference:

Application
Application
Commentary
Organism
analysis
dipstick assay for the detection of gamma-hydroxybutyrate in alcoholic beverages
Cupriavidus necator
Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli pLysS as stable fusion protein
Cupriavidus necator
General Stability
General Stability
Organism
addition of ammonium sulfate, glycerol, 2-mercaptoethanol, dithiothreitol, glutathione, or EDTA reduces activity during storage
Cupriavidus necator
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.19
-
gamma-hydroxybutyrate
pH 8.5, 23C
Cupriavidus necator
14
-
ethanol
pH 8.5, 23C
Cupriavidus necator
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40500
-
calculated from the predicted amino acid sequence of the native enzyme
Cupriavidus necator
66000
-
fusion protein, SDS-PAGE, calculated mass
Cupriavidus necator
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-hydroxybutanoate + NADP+
Cupriavidus necator
-
succinate semialdehyde + NADPH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Cupriavidus necator
-
-
-
Purification (Commentary)
Commentary
Organism
-
Cupriavidus necator
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxybutanoate + NADP+
-
656423
Cupriavidus necator
succinate semialdehyde + NADPH + H+
-
-
-
?
ethanol + NADP+
-
656423
Cupriavidus necator
ethanal + NADPH + H+
-
-
-
?
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
-80
-
stable for indefinite timespan
Cupriavidus necator
4
-
stable for at least 21 days, 60% of activity after 3.5 months
Cupriavidus necator
Application (protein specific)
Application
Commentary
Organism
analysis
dipstick assay for the detection of gamma-hydroxybutyrate in alcoholic beverages
Cupriavidus necator
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli pLysS as stable fusion protein
Cupriavidus necator
General Stability (protein specific)
General Stability
Organism
addition of ammonium sulfate, glycerol, 2-mercaptoethanol, dithiothreitol, glutathione, or EDTA reduces activity during storage
Cupriavidus necator
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.19
-
gamma-hydroxybutyrate
pH 8.5, 23C
Cupriavidus necator
14
-
ethanol
pH 8.5, 23C
Cupriavidus necator
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40500
-
calculated from the predicted amino acid sequence of the native enzyme
Cupriavidus necator
66000
-
fusion protein, SDS-PAGE, calculated mass
Cupriavidus necator
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4-hydroxybutanoate + NADP+
Cupriavidus necator
-
succinate semialdehyde + NADPH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Cupriavidus necator
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-hydroxybutanoate + NADP+
-
656423
Cupriavidus necator
succinate semialdehyde + NADPH + H+
-
-
-
?
ethanol + NADP+
-
656423
Cupriavidus necator
ethanal + NADPH + H+
-
-
-
?
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
-80
-
stable for indefinite timespan
Cupriavidus necator
4
-
stable for at least 21 days, 60% of activity after 3.5 months
Cupriavidus necator
Other publictions for EC 1.1.1.61
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737764
Yoshida
Production of 4-hydroxybutyrat ...
Porphyromonas gingivalis, Porphyromonas gingivalis ATCC 33277
Biochim. Biophys. Acta
1850
2582-2591
2015
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1
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2
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1
1
4
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10
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1
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-
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4
1
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-
-
1
1
1
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2
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1
2
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-
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-
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2
-
1
1
4
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1
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4
1
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1
1
1
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-
698903
Saito
Metabolite profiling reveals Y ...
Escherichia coli, Escherichia coli K12 BW25113
J. Biol. Chem.
284
16442-16451
2009
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1
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-
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5
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1
2
2
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4
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1
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4
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11
1
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2
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1
3
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8
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1
3
2
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1
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4
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11
1
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-
-
-
-
-
-
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-
2
2
-
-
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656423
Bravo
Reliable, sensitive, rapid and ...
Cupriavidus necator
J. Forensic Sci.
49
379-387
2004
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1
1
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1
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2
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2
1
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2
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1
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2
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2
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1
1
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2
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1
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1
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2
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2
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656170
Breitkreuz
A novel gamma-hydroxybutyrate ...
Arabidopsis thaliana
J. Biol. Chem.
278
41552-41556
2003
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1
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1
1
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4
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4
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2
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1
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1
1
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1
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4
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1
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287171
Wolff
Purification and characterizat ...
Clostridium kluyveri
Protein Expr. Purif.
6
206-212
1995
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1
4
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2
2
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2
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1
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1
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1
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4
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2
2
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1
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1
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2
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287170
Nirenberg
Enzymatic utilization of gamma ...
Pseudomonas sp.
J. Biol. Chem.
235
954-960
1960
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1
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1
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1
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