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show all sequences of 1.1.1.61

Purification and characterization of the oxygen-sensitive 4-hydroxybutanoate dehydrogenase from Clostridium kluyveri

Wolff, R.A.; Kenealy W.R.; Protein Expr. Purif. 6, 206-212 (1995)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
oxygen
-
Clostridium kluyveri
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.055
-
4-hydroxybutanoate
-
Clostridium kluyveri
0.15
-
NADH
-
Clostridium kluyveri
0.56
-
Succinic semialdehyde
-
Clostridium kluyveri
0.67
-
NAD+
-
Clostridium kluyveri
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Cu
monomeric units contains two atoms
Clostridium kluyveri
Fe
monomeric unit contains one atom
Clostridium kluyveri
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41600
-
2 * 41600, SDS-PAGE
Clostridium kluyveri
86000
-
gel filtration
Clostridium kluyveri
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Clostridium kluyveri
-
-
-
Purification (Commentary)
Commentary
Organism
-
Clostridium kluyveri
Subunits
Subunits
Commentary
Organism
dimer
2 * 41600, SDS-PAGE
Clostridium kluyveri
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1
-
reduction of 4-hydroxybutanoate
Clostridium kluyveri
9.4
-
oxidation of 4-hydroxybutanoate
Clostridium kluyveri
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
oxygen
-
Clostridium kluyveri
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.055
-
4-hydroxybutanoate
-
Clostridium kluyveri
0.15
-
NADH
-
Clostridium kluyveri
0.56
-
Succinic semialdehyde
-
Clostridium kluyveri
0.67
-
NAD+
-
Clostridium kluyveri
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Cu
monomeric units contains two atoms
Clostridium kluyveri
Fe
monomeric unit contains one atom
Clostridium kluyveri
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41600
-
2 * 41600, SDS-PAGE
Clostridium kluyveri
86000
-
gel filtration
Clostridium kluyveri
Purification (Commentary) (protein specific)
Commentary
Organism
-
Clostridium kluyveri
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 41600, SDS-PAGE
Clostridium kluyveri
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.1
-
reduction of 4-hydroxybutanoate
Clostridium kluyveri
9.4
-
oxidation of 4-hydroxybutanoate
Clostridium kluyveri
Other publictions for EC 1.1.1.61
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737764
Yoshida
Production of 4-hydroxybutyrat ...
Porphyromonas gingivalis, Porphyromonas gingivalis ATCC 33277
Biochim. Biophys. Acta
1850
2582-2591
2015
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1
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2
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1
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4
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10
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1
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1
1
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1
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1
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698903
Saito
Metabolite profiling reveals Y ...
Escherichia coli, Escherichia coli K12 BW25113
J. Biol. Chem.
284
16442-16451
2009
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1
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2
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656423
Bravo
Reliable, sensitive, rapid and ...
Cupriavidus necator
J. Forensic Sci.
49
379-387
2004
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656170
Breitkreuz
A novel gamma-hydroxybutyrate ...
Arabidopsis thaliana
J. Biol. Chem.
278
41552-41556
2003
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1
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1
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287171
Wolff
Purification and characterizat ...
Clostridium kluyveri
Protein Expr. Purif.
6
206-212
1995
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1
4
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2
2
-
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2
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1
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-
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1
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2
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2
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1
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1
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2
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287170
Nirenberg
Enzymatic utilization of gamma ...
Pseudomonas sp.
J. Biol. Chem.
235
954-960
1960
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