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show all sequences of 1.1.1.60

X-ray crystal structure of GarR - tartronate semialdehyde reductase from Salmonella typhimurium

Osipiuk, J.; Zhou, M.; Moy, S.; Collart, F.; Joachimiak, A.; J. Struct. Funct. Genomics 10, 249-253 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene garR, expression of the His6-tagged enzyme in Escherichia coli strain BL21
Salmonella enterica subsp. enterica serovar Typhimurium
Crystallization (Commentary)
Crystallization
Organism
enzyme with L-tartrate bound to the active site, sitting drop vapour diffusion method, mixing of 0.0005 ml of the protein solution, containing 107 mg/ml protein in in 10 mM HEPES pH 8.0, 200 mM NaCl, with 0.0005 ml of 0.1 M imidazole buffer pH 8.0, 1 M potassium/sodium L-tartrate and 0.2 M sodium chloride, and equilibrated at 16C over 0.135 ml of this solution, overnight, X-ray diffraction structure determination and analysis at 1.65 A resolution, the single-wavelength anomalous diffraction method
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tartronate semialdehyde + NADH
Salmonella enterica subsp. enterica serovar Typhimurium
as cofactor in the final stage of D-glycerate biosynthesis
(R)-glycerate + NAD+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Salmonella enterica subsp. enterica serovar Typhimurium
-
gene garR
-
Purification (Commentary)
Commentary
Organism
recombinant His6-tagged GarR from Escherichia coli strain BL21, the tag is removed by TEV protease, purification by nickel affinity chromatography
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
tartronate semialdehyde + NADH
as cofactor in the final stage of D-glycerate biosynthesis
699856
Salmonella enterica subsp. enterica serovar Typhimurium
(R)-glycerate + NAD+
-
-
-
?
tartronate semialdehyde + NADH + H+
-
699856
Salmonella enterica subsp. enterica serovar Typhimurium
(R)-glycerate + NAD+
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Salmonella enterica subsp. enterica serovar Typhimurium
Cloned(Commentary) (protein specific)
Commentary
Organism
gene garR, expression of the His6-tagged enzyme in Escherichia coli strain BL21
Salmonella enterica subsp. enterica serovar Typhimurium
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Salmonella enterica subsp. enterica serovar Typhimurium
Crystallization (Commentary) (protein specific)
Crystallization
Organism
enzyme with L-tartrate bound to the active site, sitting drop vapour diffusion method, mixing of 0.0005 ml of the protein solution, containing 107 mg/ml protein in in 10 mM HEPES pH 8.0, 200 mM NaCl, with 0.0005 ml of 0.1 M imidazole buffer pH 8.0, 1 M potassium/sodium L-tartrate and 0.2 M sodium chloride, and equilibrated at 16C over 0.135 ml of this solution, overnight, X-ray diffraction structure determination and analysis at 1.65 A resolution, the single-wavelength anomalous diffraction method
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tartronate semialdehyde + NADH
Salmonella enterica subsp. enterica serovar Typhimurium
as cofactor in the final stage of D-glycerate biosynthesis
(R)-glycerate + NAD+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His6-tagged GarR from Escherichia coli strain BL21, the tag is removed by TEV protease, purification by nickel affinity chromatography
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
tartronate semialdehyde + NADH
as cofactor in the final stage of D-glycerate biosynthesis
699856
Salmonella enterica subsp. enterica serovar Typhimurium
(R)-glycerate + NAD+
-
-
-
?
tartronate semialdehyde + NADH + H+
-
699856
Salmonella enterica subsp. enterica serovar Typhimurium
(R)-glycerate + NAD+
-
-
-
?
Other publictions for EC 1.1.1.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737745
Hoover
Identification of catalyticall ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
Biochim. Biophys. Acta
1834
2663-2671
2013
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726270
Liu
Tartronate semialdehyde reduct ...
Ustilago maydis
PLoS ONE
6
e16438
2011
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1
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5
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3
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699856
Osipiuk
X-ray crystal structure of Gar ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Struct. Funct. Genomics
10
249-253
2009
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287168
Njau
New developments in our unders ...
Escherichia coli, Haemophilus influenzae
Chem. Biol. Interact.
130-132
785-791
2001
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2
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2
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287167
Ijau
Novel beta-hydroxyacid dehydro ...
Escherichia coli, Haemophilus influenzae
J. Biol. Chem.
275
38780-38786
2000
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2
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287162
Van der Drift
Glyoxylate conversion by Hypho ...
Hyphomicrobium sp.
Antonie van Leeuwenhoek
49
167-172
1983
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1
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4589
Dijkhuizen
Metabolic regulation in Pseudo ...
Cupriavidus oxalaticus, Cupriavidus oxalaticus OX1
Arch. Microbiol.
116
77-83
1978
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287164
Kohn
Tartaric acid metabolism. 8. C ...
Pseudomonas putida
J. Biol. Chem.
243
4426-4433
1968
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1
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287165
Kornberg
-
Tartronic semialdehyde reducta ...
Pseudomonas putida
Methods Enzymol.
9
240-247
1966
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287166
Gotto
The metabolism of C2 compounds ...
Pseudomonas putida
Biochem. J.
81
273-284
1961
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