BRENDA - Enzyme Database show
show all sequences of 1.1.1.60

Tartronic semialdehyde reductase (crystalline)

Kornberg, H.L.; Gotto, A.M.; Methods Enzymol. 9, 240-247 (1966)
No PubMed abstract available

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
-
Pseudomonas putida
Inhibitors
Inhibitors
Commentary
Organism
Structure
fluoroacetate
-
Pseudomonas putida
glycolate
-
Pseudomonas putida
glyoxylate
-
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
0.027
NADH
-
Pseudomonas putida
0.036
0.05
NADPH
-
Pseudomonas putida
0.05
0.4
D-glycerate
-
Pseudomonas putida
0.05
0.4
tartronate semialdehyde
-
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
91000
-
sedimentation equilibrium
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tartronate semialdehyde + NAD(P)H
Pseudomonas putida
-
(R)-glycerate + NAD(P)+
-
Pseudomonas putida
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
160
-
-
Pseudomonas putida
Storage Stability
Storage Stability
Organism
2°C, suspension in ammonium sulfate, pH 7.5, 10% loss in 1 month
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
hydroxypyruvate + NAD(P)H
-
287165
Pseudomonas putida
glycerate + NAD(P)+
-
287165
Pseudomonas putida
r
malonic semialdehyde + NADH
-
287165
Pseudomonas putida
3-hydroxypropanoate + NAD+
-
287165
Pseudomonas putida
?
mesoxalic semialdehyde + NADH
-
287165
Pseudomonas putida
hydroxypyruvate + NAD+
-
287165
Pseudomonas putida
?
tartronate semialdehyde + NAD(P)H
-
287165
Pseudomonas putida
(R)-glycerate + NAD(P)+
-
287165
Pseudomonas putida
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
243
-
NADH
-
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2
8.7
-
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
NADH
-
Pseudomonas putida
NADP+
-
Pseudomonas putida
NADPH
-
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Pseudomonas putida
NADH
-
Pseudomonas putida
NADP+
-
Pseudomonas putida
NADPH
-
Pseudomonas putida
Crystallization (Commentary) (protein specific)
Crystallization
Organism
-
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
fluoroacetate
-
Pseudomonas putida
glycolate
-
Pseudomonas putida
glyoxylate
-
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
0.027
NADH
-
Pseudomonas putida
0.036
0.05
NADPH
-
Pseudomonas putida
0.05
0.4
D-glycerate
-
Pseudomonas putida
0.05
0.4
tartronate semialdehyde
-
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
91000
-
sedimentation equilibrium
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
tartronate semialdehyde + NAD(P)H
Pseudomonas putida
-
(R)-glycerate + NAD(P)+
-
Pseudomonas putida
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
160
-
-
Pseudomonas putida
Storage Stability (protein specific)
Storage Stability
Organism
2°C, suspension in ammonium sulfate, pH 7.5, 10% loss in 1 month
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
hydroxypyruvate + NAD(P)H
-
287165
Pseudomonas putida
glycerate + NAD(P)+
-
287165
Pseudomonas putida
r
malonic semialdehyde + NADH
-
287165
Pseudomonas putida
3-hydroxypropanoate + NAD+
-
287165
Pseudomonas putida
?
mesoxalic semialdehyde + NADH
-
287165
Pseudomonas putida
hydroxypyruvate + NAD+
-
287165
Pseudomonas putida
?
tartronate semialdehyde + NAD(P)H
-
287165
Pseudomonas putida
(R)-glycerate + NAD(P)+
-
287165
Pseudomonas putida
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
243
-
NADH
-
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2
8.7
-
Pseudomonas putida
Other publictions for EC 1.1.1.60
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737745
Hoover
Identification of catalyticall ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
Biochim. Biophys. Acta
1834
2663-2671
2013
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726270
Liu
Tartronate semialdehyde reduct ...
Ustilago maydis
PLoS ONE
6
e16438
2011
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1
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1
5
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-
3
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4
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2
4
1
1
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1
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3
2
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1
2
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1
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5
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3
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2
4
1
1
-
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-
1
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3
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1
1
-
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-
699856
Osipiuk
X-ray crystal structure of Gar ...
Salmonella enterica subsp. enterica serovar Typhimurium
J. Struct. Funct. Genomics
10
249-253
2009
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1
1
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1
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1
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1
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2
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1
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1
1
1
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1
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1
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2
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287168
Njau
New developments in our unders ...
Escherichia coli, Haemophilus influenzae
Chem. Biol. Interact.
130-132
785-791
2001
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2
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2
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5
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4
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2
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2
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4
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287167
Ijau
Novel beta-hydroxyacid dehydro ...
Escherichia coli, Haemophilus influenzae
J. Biol. Chem.
275
38780-38786
2000
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2
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5
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4
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2
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6
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2
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-
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-
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5
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2
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6
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-
287162
Van der Drift
Glyoxylate conversion by Hypho ...
Hyphomicrobium sp.
Antonie van Leeuwenhoek
49
167-172
1983
-
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2
1
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1
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2
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3
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1
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4
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4
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2
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1
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1
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3
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1
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4589
Dijkhuizen
Metabolic regulation in Pseudo ...
Cupriavidus oxalaticus, Cupriavidus oxalaticus OX1
Arch. Microbiol.
116
77-83
1978
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-
-
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2
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2
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2
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4
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4
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2
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2
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287164
Kohn
Tartaric acid metabolism. 8. C ...
Pseudomonas putida
J. Biol. Chem.
243
4426-4433
1968
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1
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5
5
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2
1
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1
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1
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1
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2
1
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1
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4
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4
1
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5
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5
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2
1
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1
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1
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2
1
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1
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-
-
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-
-
287165
Kornberg
-
Tartronic semialdehyde reducta ...
Pseudomonas putida
Methods Enzymol.
9
240-247
1966
-
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-
1
-
-
3
4
-
-
1
1
-
1
-
-
1
-
-
-
1
1
4
-
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1
1
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4
-
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4
1
-
-
-
3
-
4
-
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1
1
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1
-
-
1
1
4
-
-
-
-
1
1
-
-
-
-
-
-
-
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-
287166
Gotto
The metabolism of C2 compounds ...
Pseudomonas putida
Biochem. J.
81
273-284
1961
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1
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3
5
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1
1
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1
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1
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1
1
4
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1
1
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4
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1
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3
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1
1
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1
1
4
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1
1
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