Crystallization (Comment) | Organism |
---|---|
structure of mutant D121N/F245S | Weizmannia coagulans |
Protein Variants | Comment | Organism |
---|---|---|
D121N | mutation lowers the activity of the enzyme with most all the tested substrates relative to the native enzyme | Weizmannia coagulans |
D121N/F245S | mutant acquires D-lactate dehydrogenase activiy, the alteration increases the capacity of the glycerol binding site and facilitated hydrogen bonding between the S245 gamma-O and the C1 carboxylate of pyruvate | Weizmannia coagulans |
F245S | mutant acquires D-lactate dehydrogenase activiy, the alteration increases the capacity of the glycerol binding site and facilitated hydrogen bonding between the S245 gamma-O and the C1 carboxylate of pyruvate | Weizmannia coagulans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NADH | wild-type, pH 5.4, 55°C | Weizmannia coagulans | |
0.1 | - |
NADH | mutant D121N, pH 5.4, 55°C | Weizmannia coagulans | |
0.1 | - |
NADH | mutant F245S, pH 5.4, 55°C | Weizmannia coagulans | |
0.3 | - |
NADH | mutant /D121NF245S, pH 5.9, 55°C | Weizmannia coagulans | |
0.4 | - |
NAD+ | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
0.7 | - |
NAD+ | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
1 | - |
D-1,2-propanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
1.5 | - |
NAD+ | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
2.3 | - |
NAD+ | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
3 | - |
1,2-Butanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
4 | - |
1,2-Butanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
4 | - |
glycerol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
4.5 | - |
D-1,2-propanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
6.5 | - |
D-2,3-butanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
8 | - |
D-1,2-propanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
17 | - |
D-2,3-butanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
19 | - |
D-1,2-propanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
23.5 | - |
1,2-Butanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
39 | - |
D-2,3-butanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
41 | - |
glycerol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
44 | - |
1,2-Butanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
54.5 | - |
pyruvate | mutant F245S, pH 5.4, 55°C | Weizmannia coagulans | |
55.5 | - |
pyruvate | mutant /D121NF245S, pH 5.9, 55°C | Weizmannia coagulans | |
60.5 | - |
pyruvate | mutant D121N, pH 5.4, 55°C | Weizmannia coagulans | |
90 | - |
D-2,3-butanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
148 | - |
glycerol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
195 | - |
glycerol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
207 | - |
pyruvate | wild-type, pH 5.4, 55°C | Weizmannia coagulans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | 1.9fold increase in activity in presence of Zn2+ | Weizmannia coagulans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
86000 | - |
gel filtration | Weizmannia coagulans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Weizmannia coagulans | A0A150JSL8 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,2-butanediol + NAD+ | - |
Weizmannia coagulans | 1-hydroxy-2-butanone + NADH + H+ | - |
? | |
1,2-ethanediol + NAD+ | - |
Weizmannia coagulans | hydroxyacetaldehyde + NADH + H+ | - |
? | |
1,3-butanediol + NAD+ | - |
Weizmannia coagulans | 4-hydroxy-2-butanone + NADH + H+ | - |
? | |
1,4-butanediol + NAD+ | - |
Weizmannia coagulans | ? + NADH + H+ | - |
? | |
D-1,2-propanediol + NAD+ | - |
Weizmannia coagulans | hydroxyacetone + NADH + H+ | - |
? | |
D-2,3-butanediol + NAD+ | - |
Weizmannia coagulans | ? + NADH + H+ | - |
? | |
glycerol + NAD+ | - |
Weizmannia coagulans | glycerone + NADH + H+ | - |
? | |
L-1,2-propanediol + NAD+ | - |
Weizmannia coagulans | hydroxyacetone + NADH + H+ | - |
? | |
L-2,3-butanediol + NAD+ | - |
Weizmannia coagulans | ? + NADH + H+ | - |
? | |
pyruvate + NADH + H+ | - |
Weizmannia coagulans | D-lactate + NADH | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39000, calculated from sequence | Weizmannia coagulans |
Synonyms | Comment | Organism |
---|---|---|
B4100_2156 | - |
Weizmannia coagulans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 65 | mutant F245S, pH 5, reduction of pyruvate | Weizmannia coagulans |
60 | - |
mutant D121N/F245S | Weizmannia coagulans |
65 | 75 | wild-type | Weizmannia coagulans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
1 h, 95% loss of activity | Weizmannia coagulans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.5 | - |
pyruvate | mutant D121N, pH 5.4, 55°C | Weizmannia coagulans | |
10.5 | - |
D-2,3-butanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
10.5 | - |
pyruvate | wild-type, pH 5.4, 55°C | Weizmannia coagulans | |
11 | - |
glycerol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
29 | - |
D-1,2-propanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
42.5 | - |
D-1,2-propanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
42.5 | - |
glycerol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
50 | - |
1,2-Butanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
50 | - |
glycerol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
50.5 | - |
D-2,3-butanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
57.5 | - |
D-2,3-butanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
66.5 | - |
1,2-Butanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
71 | - |
1,2-Butanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
87.5 | - |
D-1,2-propanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
92 | - |
D-1,2-propanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
117.5 | - |
glycerol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
120.5 | - |
1,2-Butanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
136 | - |
D-2,3-butanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
165 | - |
pyruvate | mutant /D121NF245S, pH 5.9, 55°C | Weizmannia coagulans | |
352 | - |
pyruvate | mutant F245S, pH 5.4, 55°C | Weizmannia coagulans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
mutant F245S, reduction of pyruvate | Weizmannia coagulans |
9 | - |
wild-type | Weizmannia coagulans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
pyruvate | mutant D121N, pH 5.4, 55°C | Weizmannia coagulans | |
0.05 | - |
pyruvate | wild-type, pH 5.4, 55°C | Weizmannia coagulans | |
0.1 | - |
glycerol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
0.1 | - |
D-2,3-butanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
0.3 | - |
glycerol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
1 | - |
1,2-Butanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
1 | - |
glycerol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.5 | - |
D-2,3-butanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
1.5 | - |
D-1,2-propanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
3 | - |
pyruvate | mutant /D121NF245S, pH 5.9, 55°C | Weizmannia coagulans | |
3 | - |
1,2-Butanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
3 | - |
D-2,3-butanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
5.5 | - |
D-1,2-propanediol | mutant /D121NF245S, pH 8.9, 55°C | Weizmannia coagulans | |
6.5 | - |
pyruvate | mutant F245S, pH 5.4, 55°C | Weizmannia coagulans | |
15.5 | - |
1,2-Butanediol | mutant D121N, pH 8.9, 55°C | Weizmannia coagulans | |
20 | - |
D-1,2-propanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
21 | - |
D-2,3-butanediol | mutant F245S, pH 8.9, 55°C | Weizmannia coagulans | |
30 | - |
glycerol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
40 | - |
1,2-Butanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans | |
92 | - |
D-1,2-propanediol | wild-type, pH 8.9, 55°C | Weizmannia coagulans |