Cloned (Comment) | Organism |
---|---|
gene gldA, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain BL21(DE3) | Serratia marcescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, recombinant enzyme, SDS-PAGE | Serratia marcescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | Serratia marcescens | - |
glycerone + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | - |
- |
- |
Serratia marcescens H30 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerol + NAD+ | - |
Serratia marcescens | glycerone + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, recombinant enzyme, SDS-PAGE | Serratia marcescens |
Synonyms | Comment | Organism |
---|---|---|
GDH | - |
Serratia marcescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Serratia marcescens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 70 | temperature profile, overview | Serratia marcescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Serratia marcescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 10 | temperature profile, overview | Serratia marcescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Serratia marcescens | |
NADH | - |
Serratia marcescens |
General Information | Comment | Organism |
---|---|---|
evolution | the Serratia marcescens enzyme belongs to the type III Fe-ADH superfamily, three consecutive glycine residues belong to a 14-amino acid residue motif (GDK motif) as the coenzyme NAD(H) binding site, and three conserved histidine residues belong to a 16-residue segment that is homologous to the 15-residue stretch as the binding site of metal | Serratia marcescens |