BRENDA - Enzyme Database show
show all sequences of 1.1.1.59

Candida albicans utilizes a modified beta-oxidation pathway for the degradation of toxic propionyl-CoA

Otzen, C.; Bardl, B.; Jacobsen, I.D.; Nett, M.; Brock, M.; J. Biol. Chem. 289, 8151-8169 (2014)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
(S)-3-hydroxyisobutyrate
at pH 9.5 and 22°C
Candida albicans
0.58
-
(R)-3-Hydroxyisobutyrate
at pH 9.5 and 22°C
Candida albicans
5.37
-
3-hydroxypropanoate
at pH 9.5 and 22°C
Candida albicans
46.7
-
L-serine
at pH 9.5 and 22°C
Candida albicans
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Candida albicans
5739
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxypropanoate + NAD+
Candida albicans
preferred substrate
3-oxopropanoate + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Candida albicans
A0A1D8PQ07
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3-hydroxyisobutyrate + NAD+
-
738640
Candida albicans
3-oxoisobutyrate + NADH + H+
-
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
-
738640
Candida albicans
3-oxoisobutyrate + NADH + H+
-
-
-
?
3-hydroxypropanoate + NAD+
preferred substrate
738640
Candida albicans
3-oxopropanoate + NADH + H+
-
-
-
?
L-serine + NAD+
-
738640
Candida albicans
3-oxo-L-alanine + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 39200, calculated from amino acid sequence; x * 40000, SDS-PAGE
Candida albicans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.31
-
(R)-3-Hydroxyisobutyrate
at pH 9.5 and 22°C
Candida albicans
2.48
-
(S)-3-hydroxyisobutyrate
at pH 9.5 and 22°C
Candida albicans
9.41
-
L-serine
at pH 9.5 and 22°C
Candida albicans
30.97
-
3-hydroxypropanoate
at pH 9.5 and 22°C
Candida albicans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Candida albicans
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Candida albicans
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Candida albicans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.13
-
(S)-3-hydroxyisobutyrate
at pH 9.5 and 22°C
Candida albicans
0.58
-
(R)-3-Hydroxyisobutyrate
at pH 9.5 and 22°C
Candida albicans
5.37
-
3-hydroxypropanoate
at pH 9.5 and 22°C
Candida albicans
46.7
-
L-serine
at pH 9.5 and 22°C
Candida albicans
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
mitochondrion
-
Candida albicans
5739
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxypropanoate + NAD+
Candida albicans
preferred substrate
3-oxopropanoate + NADH + H+
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-3-hydroxyisobutyrate + NAD+
-
738640
Candida albicans
3-oxoisobutyrate + NADH + H+
-
-
-
?
(S)-3-hydroxyisobutyrate + NAD+
-
738640
Candida albicans
3-oxoisobutyrate + NADH + H+
-
-
-
?
3-hydroxypropanoate + NAD+
preferred substrate
738640
Candida albicans
3-oxopropanoate + NADH + H+
-
-
-
?
L-serine + NAD+
-
738640
Candida albicans
3-oxo-L-alanine + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 39200, calculated from amino acid sequence; x * 40000, SDS-PAGE
Candida albicans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.31
-
(R)-3-Hydroxyisobutyrate
at pH 9.5 and 22°C
Candida albicans
2.48
-
(S)-3-hydroxyisobutyrate
at pH 9.5 and 22°C
Candida albicans
9.41
-
L-serine
at pH 9.5 and 22°C
Candida albicans
30.97
-
3-hydroxypropanoate
at pH 9.5 and 22°C
Candida albicans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
9.5
-
-
Candida albicans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.202
-
L-serine
at pH 9.5 and 22°C
Candida albicans
2.26
-
(R)-3-Hydroxyisobutyrate
at pH 9.5 and 22°C
Candida albicans
5.77
-
3-hydroxypropanoate
at pH 9.5 and 22°C
Candida albicans
19.1
-
(S)-3-hydroxyisobutyrate
at pH 9.5 and 22°C
Candida albicans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.202
-
L-serine
at pH 9.5 and 22°C
Candida albicans
2.26
-
(R)-3-Hydroxyisobutyrate
at pH 9.5 and 22°C
Candida albicans
5.77
-
3-hydroxypropanoate
at pH 9.5 and 22°C
Candida albicans
19.1
-
(S)-3-hydroxyisobutyrate
at pH 9.5 and 22°C
Candida albicans
Other publictions for EC 1.1.1.59
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738640
Otzen
Candida albicans utilizes a mo ...
Candida albicans
J. Biol. Chem.
289
8151-8169
2014
-
-
-
-
-
-
-
4
1
-
-
1
-
4
-
-
-
-
-
-
-
-
4
1
-
-
-
4
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
4
1
-
-
1
-
-
-
-
-
-
-
-
4
1
-
-
-
4
1
-
-
-
-
-
-
-
4
4
695710
Yao
The catalytic property of 3-hy ...
Bacillus cereus, Bacillus cereus ATCC 14579, Bacillus cereus ATCC14579
Appl. Biochem. Biotechnol.
160
694-703
2009
-
-
1
-
-
-
1
5
-
1
1
2
-
10
-
-
1
-
-
-
1
-
5
1
1
1
2
2
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
5
-
1
1
2
-
-
-
1
-
-
1
-
5
1
1
1
2
2
1
1
-
-
-
-
-
-
2
2
655504
Qatibi
-
Anaerobic 1,3-propanediol diss ...
Desulfovibrio alcoholivorans, no activity in Desulfovibrio carbinolicus, no activity in Desulfovibrio fructosovorans
Eur. Symp. Environ. Biotechnol. , ESEB 2004, Proceedings, Oostende, Belgium, Apr. 25-28
2004
693-696
2004
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5348
Miyakoshi
-
Distribution of the methylcitr ...
Candida catenulata, Candida gropengiesseri, Candida rugosa, Priceomyces haplophilus, Schwanniomyces polymorphus
Agric. Biol. Chem.
51
2381-2387
1987
-
-
-
-
-
-
-
-
-
-
-
6
-
5
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
5
-
-
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287142
Den
Enzymatic conversion of beta-h ...
Eremothecium ashbyi, Escherichia coli, Gallus gallus, Propionibacterium freudenreichii subsp. shermanii, Sus scrofa, Tetrahymena pyriformis
J. Biol. Chem.
234
1666-1671
1959
-
-
-
-
-
-
-
2
-
-
-
6
-
6
-
-
1
-
-
4
6
1
6
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
6
-
-
-
-
-
-
2
-
-
-
6
-
-
-
1
-
4
6
1
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-