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Literature summary for 1.1.1.59 extracted from

  • Yao, T.; Xu, L.; Ying, H.; Huang, H.; Yan, M.
    The catalytic property of 3-hydroxyisobutyrate dehydrogenase from Bacillus cereus on 3-hydroxypropionate (2009), Appl. Biochem. Biotechnol., 160, 694-703.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) Bacillus cereus
MmsB gene, overexpression in Escherichia coli strain BL21, subcloning in strain DH5alpha Bacillus cereus

Inhibitors

Inhibitors Comment Organism Structure
ZnCl2 0.2 mM, 60% inhibition Bacillus cereus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetic analysis, overview Bacillus cereus
0.25
-
NADP+ pH 8.5, 37°C Bacillus cereus
2.4
-
NAD+ pH 8.5, 37°C Bacillus cereus
16.8
-
3-hydroxypropanoate pH 8.5, 37°C Bacillus cereus
16.8
-
3-hydroxypropionate pH 8.5, 37°C, KM-value for 3-hydroxyisobutyrate is approximately 20fold lower Bacillus cereus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no metal ion requirement Bacillus cereus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
x * 32000, SDS-PAGE Bacillus cereus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxypropanoate + NAD+ Bacillus cereus
-
3-oxopropanoate + NADH + H+
-
r
additional information Bacillus cereus MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, as well as 3-hydroxypropionate dehydrogenase activity ?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus cereus
-
-
-
Bacillus cereus
-
MmsB gene
-
Bacillus cereus ATCC 14579
-
-
-
Bacillus cereus ATCC 14579
-
MmsB gene
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation, hydrophobic interaction chromatography (Phenyl-Sepharose), anion exchange chromatography Bacillus cereus
native enzyme 100fold by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography Bacillus cereus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.77
-
pH 8.5, 37°C Bacillus cereus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxyisobutyrate + NAD+
-
Bacillus cereus methylmalonate semialdehyde + NADH + H+
-
r
3-hydroxypropanoate + NAD+
-
Bacillus cereus 3-oxopropanoate + NADH + H+
-
r
3-hydroxypropanoate + NAD+
-
Bacillus cereus malonate semialdehyde + NADH + H+
-
r
3-hydroxypropanoate + NADP+
-
Bacillus cereus malonate semialdehyde + NADPH + H+
-
r
additional information MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, as well as 3-hydroxypropionate dehydrogenase activity Bacillus cereus ?
-
?

Subunits

Subunits Comment Organism
? x * 32000, SDS-PAGE Bacillus cereus

Synonyms

Synonyms Comment Organism
3-hydroxyisobutyrate dehydrogenase cf. EC1.1.1.31, wide substrate specificity (hydroxyacid derivatives) Bacillus cereus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Bacillus cereus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
35 45 90% of maximal activity within this range Bacillus cereus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
30 min, purified enzyme, completely stable Bacillus cereus
45
-
stable at 45°C for 30 min, about 40% activity after 3 min at 55°C Bacillus cereus
55
-
3 min, purified enzyme, significant denaturation and inactivation Bacillus cereus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.21
-
3-hydroxypropionate pH 8.5, 37°C Bacillus cereus
0.21
-
3-hydroxypropanoate pH 8.5, 37°C Bacillus cereus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.8 9
-
Bacillus cereus

pH Range

pH Minimum pH Maximum Comment Organism
7 10
-
Bacillus cereus
7 10 activity range Bacillus cereus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus cereus
NADH
-
Bacillus cereus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0125
-
3-hydroxypropionate pH 8.5, 37°C Bacillus cereus
0.0125
-
3-hydroxypropanoate pH 8.5, 37°C Bacillus cereus