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show all sequences of 1.1.1.59

The catalytic property of 3-hydroxyisobutyrate dehydrogenase from Bacillus cereus on 3-hydroxypropionate

Yao, T.; Xu, L.; Ying, H.; Huang, H.; Yan, M.; Appl. Biochem. Biotechnol. 160, 694-703 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21 (DE3); MmsB gene, overexpression in Escherichia coli strain BL21, subcloning in strain DH5alpha
Bacillus cereus
Inhibitors
Inhibitors
Commentary
Organism
Structure
ZnCl2
0.2 mM, 60% inhibition
Bacillus cereus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state kinetic analysis, overview
Bacillus cereus
0.25
-
NADP+
pH 8.5, 37C
Bacillus cereus
2.4
-
NAD+
pH 8.5, 37C
Bacillus cereus
16.8
-
3-hydroxypropanoate
pH 8.5, 37C
Bacillus cereus
16.8
-
3-hydroxypropionate
pH 8.5, 37C, KM-value for 3-hydroxyisobutyrate is approximately 20fold lower
Bacillus cereus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ion requirement
Bacillus cereus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
x * 32000, SDS-PAGE
Bacillus cereus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxypropanoate + NAD+
Bacillus cereus
-
3-oxopropanoate + NADH + H+
-
-
r
additional information
Bacillus cereus
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, as well as 3-hydroxypropionate dehydrogenase activity
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus cereus
-
; MmsB gene
-
Bacillus cereus ATCC 14579
-
-
-
Bacillus cereus ATCC14579
-
MmsB gene
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, hydrophobic interaction chromatography (Phenyl-Sepharose), anion exchange chromatography; native enzyme 100fold by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography
Bacillus cereus
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
8.77
-
pH 8.5, 37C
Bacillus cereus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxyisobutyrate + NAD+
-
695710
Bacillus cereus
methylmalonate semialdehyde + NADH + H+
-
-
-
r
3-hydroxypropanoate + NAD+
-
695710
Bacillus cereus
3-oxopropanoate + NADH + H+
-
-
-
r
3-hydroxypropanoate + NAD+
-
695710
Bacillus cereus
malonate semialdehyde + NADH + H+
-
-
-
r
3-hydroxypropanoate + NADP+
-
695710
Bacillus cereus
malonate semialdehyde + NADPH + H+
-
-
-
r
additional information
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, as well as 3-hydroxypropionate dehydrogenase activity
695710
Bacillus cereus
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 32000, SDS-PAGE
Bacillus cereus
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
-
Bacillus cereus
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
35
45
90% of maximal activity within this range
Bacillus cereus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
45
-
30 min, purified enzyme, completely stable; stable at 45C for 30 min, about 40% activity after 3 min at 55C
Bacillus cereus
55
-
3 min, purified enzyme, significant denaturation and inactivation
Bacillus cereus
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.21
-
3-hydroxypropanoate
pH 8.5, 37C
Bacillus cereus
0.21
-
3-hydroxypropionate
pH 8.5, 37C
Bacillus cereus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
9
-
Bacillus cereus
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
10
; activity range
Bacillus cereus
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Bacillus cereus
NADH
-
Bacillus cereus
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21 (DE3); MmsB gene, overexpression in Escherichia coli strain BL21, subcloning in strain DH5alpha
Bacillus cereus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Bacillus cereus
NADH
-
Bacillus cereus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
ZnCl2
0.2 mM, 60% inhibition
Bacillus cereus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
steady-state kinetic analysis, overview
Bacillus cereus
0.25
-
NADP+
pH 8.5, 37C
Bacillus cereus
2.4
-
NAD+
pH 8.5, 37C
Bacillus cereus
16.8
-
3-hydroxypropanoate
pH 8.5, 37C
Bacillus cereus
16.8
-
3-hydroxypropionate
pH 8.5, 37C, KM-value for 3-hydroxyisobutyrate is approximately 20fold lower
Bacillus cereus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no metal ion requirement
Bacillus cereus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
32000
-
x * 32000, SDS-PAGE
Bacillus cereus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxypropanoate + NAD+
Bacillus cereus
-
3-oxopropanoate + NADH + H+
-
-
r
additional information
Bacillus cereus
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, as well as 3-hydroxypropionate dehydrogenase activity
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, hydrophobic interaction chromatography (Phenyl-Sepharose), anion exchange chromatography; native enzyme 100fold by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatography
Bacillus cereus
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
8.77
-
pH 8.5, 37C
Bacillus cereus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxyisobutyrate + NAD+
-
695710
Bacillus cereus
methylmalonate semialdehyde + NADH + H+
-
-
-
r
3-hydroxypropanoate + NAD+
-
695710
Bacillus cereus
3-oxopropanoate + NADH + H+
-
-
-
r
3-hydroxypropanoate + NAD+
-
695710
Bacillus cereus
malonate semialdehyde + NADH + H+
-
-
-
r
3-hydroxypropanoate + NADP+
-
695710
Bacillus cereus
malonate semialdehyde + NADPH + H+
-
-
-
r
additional information
MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase, EC 1.1.1.31, as well as 3-hydroxypropionate dehydrogenase activity
695710
Bacillus cereus
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 32000, SDS-PAGE
Bacillus cereus
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
-
Bacillus cereus
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
35
45
90% of maximal activity within this range
Bacillus cereus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
45
-
30 min, purified enzyme, completely stable; stable at 45C for 30 min, about 40% activity after 3 min at 55C
Bacillus cereus
55
-
3 min, purified enzyme, significant denaturation and inactivation
Bacillus cereus
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.21
-
3-hydroxypropanoate
pH 8.5, 37C
Bacillus cereus
0.21
-
3-hydroxypropionate
pH 8.5, 37C
Bacillus cereus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8.8
9
-
Bacillus cereus
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
10
; activity range
Bacillus cereus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0125
-
3-hydroxypropanoate
pH 8.5, 37C
Bacillus cereus
0.0125
-
3-hydroxypropionate
pH 8.5, 37C
Bacillus cereus
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.0125
-
3-hydroxypropanoate
pH 8.5, 37C
Bacillus cereus
0.0125
-
3-hydroxypropionate
pH 8.5, 37C
Bacillus cereus
Other publictions for EC 1.1.1.59
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
738640
Otzen
Candida albicans utilizes a mo ...
Candida albicans
J. Biol. Chem.
289
8151-8169
2014
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4
1
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1
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4
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1
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1
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4
1
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4
1
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4
1
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-
4
4
695710
Yao
The catalytic property of 3-hy ...
Bacillus cereus, Bacillus cereus ATCC 14579, Bacillus cereus ATCC14579
Appl. Biochem. Biotechnol.
160
694-703
2009
-
-
1
-
-
-
1
5
-
1
1
2
-
10
-
-
1
-
-
-
1
-
5
1
1
1
2
2
1
1
-
2
-
-
-
-
-
1
2
-
-
-
-
1
-
5
-
1
1
2
-
-
-
1
-
-
1
-
5
1
1
1
2
2
1
1
-
-
-
-
-
-
2
2
655504
Qatibi
-
Anaerobic 1,3-propanediol diss ...
Desulfovibrio alcoholivorans, no activity in Desulfovibrio carbinolicus, no activity in Desulfovibrio fructosovorans
Eur. Symp. Environ. Biotechnol. , ESEB 2004, Proceedings, Oostende, Belgium, Apr. 25-28
2004
693-696
2004
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1
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3
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1
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2
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2
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1
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-
1
-
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5348
Miyakoshi
-
Distribution of the methylcitr ...
Candida catenulata, Candida gropengiesseri, Candida rugosa, Priceomyces haplophilus, Schwanniomyces polymorphus
Agric. Biol. Chem.
51
2381-2387
1987
-
-
-
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-
-
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-
-
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-
6
-
5
-
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-
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-
-
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6
-
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-
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5
-
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5
-
-
-
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-
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-
-
6
-
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-
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6
-
-
-
-
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-
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-
-
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287142
Den
Enzymatic conversion of beta-h ...
Eremothecium ashbyi, Escherichia coli, Gallus gallus, Propionibacterium freudenreichii subsp. shermanii, Sus scrofa, Tetrahymena pyriformis
J. Biol. Chem.
234
1666-1671
1959
-
-
-
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2
-
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6
-
6
-
-
1
-
-
4
6
1
6
-
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1
-
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6
-
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6
-
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2
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6
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1
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4
6
1
6
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1
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