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show all sequences of 1.1.1.58

Toward pectin fermentation by Saccharomyces cerevisiae: expression of the first two steps of a bacterial pathway for D-galacturonate metabolism

Huisjes, E.H.; Luttik, M.A.; Almering, M.J.; Bisschops, M.M.; Dang, D.H.; Kleerebezem, M.; Siezen, R.; van Maris, A.J.; Pronk, J.T.; J. Biotechnol. 162, 303-310 (2012)

Data extracted from this reference:

Application
Application
Commentary
Organism
synthesis
expression of Lactococcus lactis uxaB and uxaC genes encoding D-tagaturonate reductase and D-galacturonate isomerase, in Saccharomyces cerevisiae to investigate in vivo activity of the first steps of the D-galacturonate pathway. Although D-tagaturonate reductase could, in principle, provide an alternative means for re-oxidizing cytosolic NADH, addition of D-galacturonate does not restore anaerobic growth, possibly due to absence of a functional D-altronate exporter in Saccharomyces cerevisiae
Lactococcus lactis
Cloned(Commentary)
Commentary
Organism
expression in Saccharomyces cerevisiae
Lactococcus lactis
Engineering
Amino acid exchange
Commentary
Organism
additional information
expression of Lactococcus lactis uxaB and uxaC genes encoding D-tagaturonate reductase and D-galacturonate isomerase, in Saccharomyces cerevisiae to investigate in vivo activity of the first steps of the D-galacturonate pathway. Although D-tagaturonate reductase could, in principle, provide an alternative means for re-oxidizing cytosolic NADH, addition of D-galacturonate does not restore anaerobic growth, possibly due to absence of a functional D-altronate exporter in Saccharomyces cerevisiae
Lactococcus lactis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lactococcus lactis
A9QSF6
-
-
Lactococcus lactis KF147
A9QSF6
-
-
Application (protein specific)
Application
Commentary
Organism
synthesis
expression of Lactococcus lactis uxaB and uxaC genes encoding D-tagaturonate reductase and D-galacturonate isomerase, in Saccharomyces cerevisiae to investigate in vivo activity of the first steps of the D-galacturonate pathway. Although D-tagaturonate reductase could, in principle, provide an alternative means for re-oxidizing cytosolic NADH, addition of D-galacturonate does not restore anaerobic growth, possibly due to absence of a functional D-altronate exporter in Saccharomyces cerevisiae
Lactococcus lactis
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Saccharomyces cerevisiae
Lactococcus lactis
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
expression of Lactococcus lactis uxaB and uxaC genes encoding D-tagaturonate reductase and D-galacturonate isomerase, in Saccharomyces cerevisiae to investigate in vivo activity of the first steps of the D-galacturonate pathway. Although D-tagaturonate reductase could, in principle, provide an alternative means for re-oxidizing cytosolic NADH, addition of D-galacturonate does not restore anaerobic growth, possibly due to absence of a functional D-altronate exporter in Saccharomyces cerevisiae
Lactococcus lactis
Other publictions for EC 1.1.1.58
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725585
Huisjes
Toward pectin fermentation by ...
Lactococcus lactis, Lactococcus lactis KF147
J. Biotechnol.
162
303-310
2012
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1
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1
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5
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287137
Gierschner
-
D-galacturonate and D-tagaturo ...
Escherichia coli, Paenibacillus polymyxa
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
6
313-320
1984
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-
-
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2
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2
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2
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1
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2
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1
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1
1
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2
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2
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1
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2
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1
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1
1
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287139
Blanco
Construction of hybrid plasmid ...
Escherichia coli, Escherichia coli K12
J. Bacteriol.
153
747-755
1983
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1
-
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2
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163
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2
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287129
Portalier
D-Mannonate and D-altronate-NA ...
Escherichia coli
Methods Enzymol.
89
210-218
1982
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-
-
-
-
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1
4
-
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1
-
1
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1
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2
1
1
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1
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2
1
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3
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3
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1
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4
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1
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1
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2
1
1
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1
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2
1
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287130
Portalier
Colorimetric determinations of ...
Escherichia coli, Escherichia coli K12
Biochim. Biophys. Acta
289
19-27
1972
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1
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2
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2
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162
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4
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4
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287138
Portalier
D-Altronate: NAD-oxidoreductas ...
Escherichia coli
Eur. J. Biochem.
26
50-61
1972
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-
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7
3
4
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1
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1
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1
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1
1
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2
1
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4
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4
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7
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3
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4
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1
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1
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2
1
1
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2
1
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287140
Portalier
D-Altronate: NAD-oxidoreductas ...
Escherichia coli, Escherichia coli K12
Eur. J. Biochem.
30
211-219
1972
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4
4
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2
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162
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2
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287128
Hickman
Uronic acid metabolism in bact ...
Escherichia coli
J. Biol. Chem.
235
1566-1570
1960
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1
1
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1
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1
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2
1
1
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1
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2
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1
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1
1
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1
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