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D-Mannonate: NAD oxidoreductase from Escherichia coli K12. Purification, properties and specificity

Data extracted from this reference:

Inhibitors

Commentary

Organism

Structure

ATP

non-competitive

Escherichia coli

D-altronate

competitive at low concentrations; non-competitive

Escherichia coli

additional information

product inhibition with all products in forward and reverse reaction

Escherichia coli

p-chloromercuribenzoate

complete inhibition at 0.005 mM, non-competive, Ki: 0.0001 mM

Escherichia coli

tagaturonate

competitive, inhibitory on forward reaction

Escherichia coli

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.5

-

D-fructuronate

-

Escherichia coli

0.9

-

D-tagaturonate

-

Escherichia coli

1.8

-

D-mannonate

-

Escherichia coli

Inhibitors

Commentary

Organism

Structure

ATP

non-competitive

Escherichia coli

D-altronate

competitive at low concentrations; non-competitive

Escherichia coli

additional information

product inhibition with all products in forward and reverse reaction

Escherichia coli

p-chloromercuribenzoate

complete inhibition at 0.005 mM, non-competive, Ki: 0.0001 mM

Escherichia coli

tagaturonate

competitive, inhibitory on forward reaction

Escherichia coli

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

0.5

-

D-fructuronate

-

Escherichia coli

0.9

-

D-tagaturonate

-

Escherichia coli

1.8

-

D-mannonate

-

Escherichia coli

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

287129

Portalier

D-Mannonate and D-altronate-NA ...

Escherichia coli

Methods Enzymol.

89

210-218

1982

-

-

-

-

-

-

1

2

-

-

-

1

-

1

-

-

1

-

-

-

2

1

3

-

-

-

1

-

2

-

-

1

-

-

-

-

-

-

1

-

-

-

-

1

-

2

-

-

-

1

-

-

-

1

-

-

2

1

3

-

-

-

1

-

2

-

-

-

-

-

-

-

-

-

287131

Mandrand-Berthelot

Hit-and-run mechanism for D-gl ...

Escherichia coli

Biochim. Biophys. Acta

483

6-23

1977

-

-

-

-

-

-

5

-

-

-

-

1

-

1

-

-

1

-

-

-

2

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

5

-

-

-

-

-

1

-

-

-

1

-

-

2

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

287130

Portalier

Colorimetric determinations of ...

Escherichia coli

Biochim. Biophys. Acta

289

19-27

1972

-

-

-

-

-

-

-

1

-

-

-

2

-

1

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

2

-

-

-

-

-

-

-

-

4

-

-

-

-

-

-

-

-

-

-

-

-

-

-

-

287132

Portalier

D-Mannonate: NAD-oxidoreductas ...

Escherichia coli

Eur. J. Biochem.

30

220-233

1972

-

-

-

-

-

-

3

-

-

-

-

1

-

1

-

-

1

-

-

-

-

-

1

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

3

-

-

-

-

-

1

-

-

-

1

-

-

-

-

1

-

-

-

-

-

2

-

-

-

-

-

-

-

-

-

287133

Portalier

D-Mannonate: NAD oxidoreductas ...

Escherichia coli

Eur. J. Biochem.

26

290-300

1972

-

-

-

-

-

-

5

3

-

-

-

1

-

1

-

-

1

-

-

-

2

-

2

-

-

-

1

-

2

-

-

-

-

-

-

-

-

-

-

-

-

-

-

5

-

3

-

-

-

1

-

-

-

1

-

-

2

-

2

-

-

-

1

-

2

-

-

-

-

-

-

-

-

-

287128

Hickman

Uronic acid metabolism in bact ...

Escherichia coli

J. Biol. Chem.

235

1566-1570

1960

2

-

-

-

-

3

1

1

-

-

-

1

-

1

-

-

1

-

-

-

2

1

2

-

-

-

-

-

1

-

1

2

-

-

-

2

-

-

2

-

-

3

-

1

-

1

-

-

-

1

-

-

-

1

-

-

2

1

2

-

-

-

-

-

1

-

1

-

-

-

-

-

-

-