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show all sequences of 1.1.1.57

Uronic acid metabolism in bacteria. II Purification and properties of D-altronic acid and D-mannonic acid dehydrogenase in Escherichia coli

Hickman, J.; Ashwell, G.; J. Biol. Chem. 235, 1566-1570 (1960)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
-
Escherichia coli
EDTA
-
Escherichia coli
General Stability
General Stability
Organism
rapid loss of activity upon dialysis and mild heating
Escherichia coli
stabilized by 1 mM cysteine at pH 7
Escherichia coli
unstable on freezing and thawing
Escherichia coli
Inhibitors
Inhibitors
Commentary
Organism
Structure
p-chloromercuribenzoate
complete inhibition at 0.03 mM
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
D-fructuronate
-
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-mannonate + NAD+
Escherichia coli
-
D-fructuronate + NADH
-
Escherichia coli
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
26
-
crude extract
Escherichia coli
770
-
purified enzyme
Escherichia coli
Storage Stability
Storage Stability
Organism
3°C, pH 7, 1 mM cysteine, stable for 1-2 weeks
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-altronate + NAD+
approximately equal activity towards both fructuronate and tagaturonate in the forward reaction, and towards D-mannonate and D-altronate in the reverse reaction
287128
Escherichia coli
D-tagaturonate + NADH
-
287128
Escherichia coli
r
D-mannonate + NAD+
-
287128
Escherichia coli
D-fructuronate + NADH
-
287128
Escherichia coli
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Escherichia coli
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
-
unstable at lower pH
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli
NADH
-
Escherichia coli
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
2-mercaptoethanol
-
Escherichia coli
EDTA
-
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Escherichia coli
NADH
-
Escherichia coli
General Stability (protein specific)
General Stability
Organism
rapid loss of activity upon dialysis and mild heating
Escherichia coli
stabilized by 1 mM cysteine at pH 7
Escherichia coli
unstable on freezing and thawing
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
p-chloromercuribenzoate
complete inhibition at 0.03 mM
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1
-
D-fructuronate
-
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
D-mannonate + NAD+
Escherichia coli
-
D-fructuronate + NADH
-
Escherichia coli
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
26
-
crude extract
Escherichia coli
770
-
purified enzyme
Escherichia coli
Storage Stability (protein specific)
Storage Stability
Organism
3°C, pH 7, 1 mM cysteine, stable for 1-2 weeks
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-altronate + NAD+
approximately equal activity towards both fructuronate and tagaturonate in the forward reaction, and towards D-mannonate and D-altronate in the reverse reaction
287128
Escherichia coli
D-tagaturonate + NADH
-
287128
Escherichia coli
r
D-mannonate + NAD+
-
287128
Escherichia coli
D-fructuronate + NADH
-
287128
Escherichia coli
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6
-
-
Escherichia coli
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6.5
-
unstable at lower pH
Escherichia coli
Other publictions for EC 1.1.1.57
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
287129
Portalier
D-Mannonate and D-altronate-NA ...
Escherichia coli
Methods Enzymol.
89
210-218
1982
-
-
-
-
-
-
1
2
-
-
-
1
-
1
-
-
1
-
-
-
2
1
3
-
-
-
1
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
2
-
-
-
1
-
-
-
1
-
-
2
1
3
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
287131
Mandrand-Berthelot
Hit-and-run mechanism for D-gl ...
Escherichia coli
Biochim. Biophys. Acta
483
6-23
1977
-
-
-
-
-
-
5
-
-
-
-
1
-
1
-
-
1
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
-
1
-
-
-
1
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287130
Portalier
Colorimetric determinations of ...
Escherichia coli
Biochim. Biophys. Acta
289
19-27
1972
-
-
-
-
-
-
-
1
-
-
-
2
-
1
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
2
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287132
Portalier
D-Mannonate: NAD-oxidoreductas ...
Escherichia coli
Eur. J. Biochem.
30
220-233
1972
-
-
-
-
-
-
3
-
-
-
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
287133
Portalier
D-Mannonate: NAD oxidoreductas ...
Escherichia coli
Eur. J. Biochem.
26
290-300
1972
-
-
-
-
-
-
5
3
-
-
-
1
-
1
-
-
1
-
-
-
2
-
2
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
3
-
-
-
1
-
-
-
1
-
-
2
-
2
-
-
-
1
-
2
-
-
-
-
-
-
-
-
-
287128
Hickman
Uronic acid metabolism in bact ...
Escherichia coli
J. Biol. Chem.
235
1566-1570
1960
2
-
-
-
-
3
1
1
-
-
-
1
-
1
-
-
1
-
-
-
2
1
2
-
-
-
-
-
1
-
1
2
-
-
-
2
-
-
2
-
-
3
-
1
-
1
-
-
-
1
-
-
-
1
-
-
2
1
2
-
-
-
-
-
1
-
1
-
-
-
-
-
-
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