Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Providencia alcalifaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | about 20% residual activity at 1 mM | Providencia alcalifaciens | |
Zn2+ | about 43% residual activity at 1 mM | Providencia alcalifaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.042 | - |
NAD+ | at pH 10.0 and 35°C | Providencia alcalifaciens | |
13.9 | - |
ribitol | at pH 10.0 and 35°C | Providencia alcalifaciens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | about 126.8% activity at 1 mM | Providencia alcalifaciens | |
Ca2+ | about 113.5% activity at 1 mM | Providencia alcalifaciens | |
Co2+ | about 111% activity at 1 mM | Providencia alcalifaciens | |
Mg2+ | about 114.8% activity at 1 mM | Providencia alcalifaciens | |
additional information | not influenced by Mn2+ | Providencia alcalifaciens | |
Ni2+ | about 108% activity at 1 mM | Providencia alcalifaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
4 * 25000, SDS-PAGE | Providencia alcalifaciens |
26650 | - |
- |
Providencia alcalifaciens |
104000 | - |
gel filtration | Providencia alcalifaciens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ribitol + NAD+ | Providencia alcalifaciens | high activity | D-ribulose + NADH + H+ | - |
? | |
ribitol + NAD+ | Providencia alcalifaciens RIMD 1656011 | high activity | D-ribulose + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Providencia alcalifaciens | - |
- |
- |
Providencia alcalifaciens RIMD 1656011 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
nickel-charged Sepharose resin column chromatography | Providencia alcalifaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-psicose + NADH + H+ | - |
Providencia alcalifaciens | allitol + NAD+ | - |
? | |
D-psicose + NADH + H+ | - |
Providencia alcalifaciens RIMD 1656011 | allitol + NAD+ | - |
? | |
additional information | the enzyme shows 8.6% activity with allitol, low activity with xylitol (1.8%) and no activity with D-sorbitol, L-arabitol, galactitol, D-mannitol, inositol and glycerol | Providencia alcalifaciens | ? | - |
? | |
additional information | the enzyme shows 8.6% activity with allitol, low activity with xylitol (1.8%) and no activity with D-sorbitol, L-arabitol, galactitol, D-mannitol, inositol and glycerol | Providencia alcalifaciens RIMD 1656011 | ? | - |
? | |
ribitol + NAD+ | high activity | Providencia alcalifaciens | D-ribulose + NADH + H+ | - |
? | |
ribitol + NAD+ | high activity | Providencia alcalifaciens RIMD 1656011 | D-ribulose + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 25000, SDS-PAGE | Providencia alcalifaciens |
homotetramer | 4 * 25928, calculated from amino acid sequence | Providencia alcalifaciens |
homotetramer | 4 * 26650, liquid chromatography/mass spectrometry | Providencia alcalifaciens |
Synonyms | Comment | Organism |
---|---|---|
RDH | - |
Providencia alcalifaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Providencia alcalifaciens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | about 90% activity at 25°C, 100% activity at 35°C, about 80% activity at 40°C, about 55% activity at 45°C | Providencia alcalifaciens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 50 | the enzyme retains 72, 72, 48 and 0% of its initial activity after 4 h at 25, 30, 40 and 50°C, respectively | Providencia alcalifaciens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
10 | - |
ribitol | at pH 10.0 and 35°C | Providencia alcalifaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10 | - |
- |
Providencia alcalifaciens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
9 | 11 | the enzyme shows about 25% activity at pH 9.0, while at pH 10.5 and 11.0 the activity is 85.6 and 68.11% respectively of that at pH 10.0 | Providencia alcalifaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Providencia alcalifaciens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.71 | - |
ribitol | at pH 10.0 and 35°C | Providencia alcalifaciens |