BRENDA - Enzyme Database show
show all sequences of 1.1.1.56

Cloning and characterization of a new ribitol dehydrogenase from Providencia alcalifaciens RIMD 1656011

Hassanin, H.A.; Wang, X.; Mu, W.; Zhang, T.; Jiang, B.; J. Sci. Food Agric. 96, 2917-2924 (2016)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Providencia alcalifaciens
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
about 20% residual activity at 1 mM
Providencia alcalifaciens
Zn2+
about 43% residual activity at 1 mM
Providencia alcalifaciens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.042
-
NAD+
at pH 10.0 and 35°C
Providencia alcalifaciens
13.9
-
ribitol
at pH 10.0 and 35°C
Providencia alcalifaciens
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ba2+
about 126.8% activity at 1 mM
Providencia alcalifaciens
Ca2+
about 113.5% activity at 1 mM
Providencia alcalifaciens
Co2+
about 111% activity at 1 mM
Providencia alcalifaciens
Mg2+
about 114.8% activity at 1 mM
Providencia alcalifaciens
additional information
not influenced by Mn2+
Providencia alcalifaciens
Ni2+
about 108% activity at 1 mM
Providencia alcalifaciens
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
104000
-
gel filtration
Providencia alcalifaciens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ribitol + NAD+
Providencia alcalifaciens
high activity
D-ribulose + NADH + H+
-
-
?
ribitol + NAD+
Providencia alcalifaciens RIMD 1656011
high activity
D-ribulose + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Providencia alcalifaciens
-
-
-
Providencia alcalifaciens RIMD 1656011
-
-
-
Purification (Commentary)
Commentary
Organism
nickel-charged Sepharose resin column chromatography
Providencia alcalifaciens
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-psicose + NADH + H+
-
738926
Providencia alcalifaciens
allitol + NAD+
-
-
-
?
D-psicose + NADH + H+
-
738926
Providencia alcalifaciens RIMD 1656011
allitol + NAD+
-
-
-
?
additional information
the enzyme shows 8.6% activity with allitol, low activity with xylitol (1.8%) and no activity with D-sorbitol, L-arabitol, galactitol, D-mannitol, inositol and glycerol
738926
Providencia alcalifaciens
?
-
-
-
-
additional information
the enzyme shows 8.6% activity with allitol, low activity with xylitol (1.8%) and no activity with D-sorbitol, L-arabitol, galactitol, D-mannitol, inositol and glycerol
738926
Providencia alcalifaciens RIMD 1656011
?
-
-
-
-
ribitol + NAD+
high activity
738926
Providencia alcalifaciens
D-ribulose + NADH + H+
-
-
-
?
ribitol + NAD+
high activity
738926
Providencia alcalifaciens RIMD 1656011
D-ribulose + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 25000, SDS-PAGE; 4 * 25928, calculated from amino acid sequence; 4 * 26650, liquid chromatography/mass spectrometry
Providencia alcalifaciens
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Providencia alcalifaciens
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
45
about 90% activity at 25°C, 100% activity at 35°C, about 80% activity at 40°C, about 55% activity at 45°C
Providencia alcalifaciens
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
25
50
the enzyme retains 72, 72, 48 and 0% of its initial activity after 4 h at 25, 30, 40 and 50°C, respectively
Providencia alcalifaciens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
10
-
ribitol
at pH 10.0 and 35°C
Providencia alcalifaciens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
-
Providencia alcalifaciens
pH Range
pH Minimum
pH Maximum
Commentary
Organism
9
11
the enzyme shows about 25% activity at pH 9.0, while at pH 10.5 and 11.0 the activity is 85.6 and 68.11% respectively of that at pH 10.0
Providencia alcalifaciens
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Providencia alcalifaciens
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21(DE3) cells
Providencia alcalifaciens
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Providencia alcalifaciens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
about 20% residual activity at 1 mM
Providencia alcalifaciens
Zn2+
about 43% residual activity at 1 mM
Providencia alcalifaciens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.042
-
NAD+
at pH 10.0 and 35°C
Providencia alcalifaciens
13.9
-
ribitol
at pH 10.0 and 35°C
Providencia alcalifaciens
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ba2+
about 126.8% activity at 1 mM
Providencia alcalifaciens
Ca2+
about 113.5% activity at 1 mM
Providencia alcalifaciens
Co2+
about 111% activity at 1 mM
Providencia alcalifaciens
Mg2+
about 114.8% activity at 1 mM
Providencia alcalifaciens
additional information
not influenced by Mn2+
Providencia alcalifaciens
Ni2+
about 108% activity at 1 mM
Providencia alcalifaciens
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
104000
-
gel filtration
Providencia alcalifaciens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ribitol + NAD+
Providencia alcalifaciens
high activity
D-ribulose + NADH + H+
-
-
?
ribitol + NAD+
Providencia alcalifaciens RIMD 1656011
high activity
D-ribulose + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
nickel-charged Sepharose resin column chromatography
Providencia alcalifaciens
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D-psicose + NADH + H+
-
738926
Providencia alcalifaciens
allitol + NAD+
-
-
-
?
D-psicose + NADH + H+
-
738926
Providencia alcalifaciens RIMD 1656011
allitol + NAD+
-
-
-
?
additional information
the enzyme shows 8.6% activity with allitol, low activity with xylitol (1.8%) and no activity with D-sorbitol, L-arabitol, galactitol, D-mannitol, inositol and glycerol
738926
Providencia alcalifaciens
?
-
-
-
-
additional information
the enzyme shows 8.6% activity with allitol, low activity with xylitol (1.8%) and no activity with D-sorbitol, L-arabitol, galactitol, D-mannitol, inositol and glycerol
738926
Providencia alcalifaciens RIMD 1656011
?
-
-
-
-
ribitol + NAD+
high activity
738926
Providencia alcalifaciens
D-ribulose + NADH + H+
-
-
-
?
ribitol + NAD+
high activity
738926
Providencia alcalifaciens RIMD 1656011
D-ribulose + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 25000, SDS-PAGE; 4 * 25928, calculated from amino acid sequence; 4 * 26650, liquid chromatography/mass spectrometry
Providencia alcalifaciens
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
35
-
-
Providencia alcalifaciens
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
25
45
about 90% activity at 25°C, 100% activity at 35°C, about 80% activity at 40°C, about 55% activity at 45°C
Providencia alcalifaciens
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
25
50
the enzyme retains 72, 72, 48 and 0% of its initial activity after 4 h at 25, 30, 40 and 50°C, respectively
Providencia alcalifaciens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
10
-
ribitol
at pH 10.0 and 35°C
Providencia alcalifaciens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
10
-
-
Providencia alcalifaciens
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
9
11
the enzyme shows about 25% activity at pH 9.0, while at pH 10.5 and 11.0 the activity is 85.6 and 68.11% respectively of that at pH 10.0
Providencia alcalifaciens
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.71
-
ribitol
at pH 10.0 and 35°C
Providencia alcalifaciens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
0.71
-
ribitol
at pH 10.0 and 35°C
Providencia alcalifaciens
Other publictions for EC 1.1.1.56
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737628
Singh
-
D-Ribulose production by a rib ...
Enterobacter aerogenes
Biochem. Eng. J.
109
189-196
2016
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738926
Hassanin
Cloning and characterization o ...
Providencia alcalifaciens, Providencia alcalifaciens RIMD 1656011
J. Sci. Food Agric.
96
2917-2924
2016
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1
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2
2
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6
1
2
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2
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1
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6
1
1
1
1
1
1
1
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1
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1
1
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2
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2
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6
1
2
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1
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6
1
1
1
1
1
1
1
-
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1
1
738134
Singh
An efficient ribitol-specific ...
Enterobacter aerogenes, Enterobacter aerogenes KCTC 2190
Enzyme Microb. Technol.
72
56-64
2015
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1
-
-
-
6
2
-
4
1
2
-
5
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1
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4
1
1
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1
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1
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1
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6
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2
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1
2
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1
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4
1
1
1
1
-
1
-
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1
1
287127
Adachi
Membrane-bound sugar alcohol d ...
Gluconobacter oxydans
Biosci. Biotechnol. Biochem.
65
115-125
2001
-
-
-
1
-
-
-
6
1
-
2
-
-
3
-
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1
-
-
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-
4
1
-
1
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2
-
2
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2
1
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6
1
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2
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1
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4
1
-
1
-
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-
2
-
-
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-
-
-
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-
287126
Muniruzzaman
-
Purification and characterizat ...
Pantoea agglomerans
J. Ferment. Bioeng.
79
496-498
1995
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5
7
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2
2
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1
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1
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7
1
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1
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1
1
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1
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5
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7
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2
2
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1
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1
-
7
1
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1
-
1
-
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-
287125
Kahle
Pentitol metabolism of Rodobac ...
Rhodobacter sphaeroides
J. Gen. Microbiol.
138
1277-1281
1992
-
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-
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5
-
1
2
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4
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1
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1
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5
1
1
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1
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2
2
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2
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2
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5
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1
2
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1
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1
-
5
1
1
-
1
-
2
2
-
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-
-
-
-
-
287115
Dothie
Ribitol dehydrogenase of Klebs ...
Enterobacter aerogenes
Biochem. J.
230
569-578
1985
-
-
-
-
1
3
-
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1
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3
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2
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1
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287116
Loviny
Ribitol dehydrogenase of Klebs ...
Enterobacter aerogenes
Biochem. J.
230
579-585
1985
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1
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1
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-
287117
Szumiko
The possible occurrence of zin ...
Mycobacterium sp.
Acta Biochim. Pol.
31
401-408
1984
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1
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1
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1
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287118
Fromm
-
D-Ribulose ...
Enterobacter aerogenes
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
6
432-437
1984
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1
1
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2
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287119
Jörnvall
Extended superfamily of short ...
Enterobacter aerogenes
FEBS Lett.
165
190-196
1984
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1
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1
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287120
Burleigh
A comparison of wild-type and ...
Enterobacter aerogenes
Biochem. J.
143
341-352
1974
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2
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3
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1
1
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1
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1
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1
2
3
2
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3
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2
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2
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2
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3
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1
1
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1
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1
2
3
2
-
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3
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-
287121
Taylor
Ribitol dehydrogenase from Kle ...
Enterobacter aerogenes
Biochem. J.
141
693-700
1974
-
-
-
-
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2
6
3
-
1
2
-
-
1
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1
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1
2
2
1
1
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1
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1
2
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2
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2
-
6
-
3
-
1
2
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1
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1
2
2
1
1
-
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1
-
1
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-
-
287122
Charnetzky
Ribitol catabolic pathway in K ...
Enterobacter aerogenes
J. Bacteriol.
119
162-169
1974
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1
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1
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1
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1
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287123
Fossitt
-
Pentitol dehydrogenases of Aer ...
Enterobacter aerogenes
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1966
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287124
Nordlie
Ribitol dehydrogenase. II. Stu ...
Enterobacter aerogenes
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2523-2531
1959
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