BRENDA - Enzyme Database show
show all sequences of 1.1.1.56

An efficient ribitol-specific dehydrogenase from Enterobacter aerogenes

Singh, R.; Singh, R.; Kim, I.W.; Sigdel, S.; Kalia, V.C.; Kang, Y.C.; Lee, J.K.; Enzyme Microb. Technol. 72, 56-64 (2015)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
Klebsiella aerogenes
Inhibitors
Inhibitors
Commentary
Organism
Structure
Cu2+
50.5% residual activity at 5 mM
Klebsiella aerogenes
EDTA
the enzyme activity is mostly lost (about 97%) after EDTA treatment
Klebsiella aerogenes
Fe3+
27.2% residual activity at 5 mM
Klebsiella aerogenes
Hg2+
complete inhibition at 1 mM
Klebsiella aerogenes
K+
73.6% residual activity at 5 mM
Klebsiella aerogenes
Zn2+
61.9% residual activity at 5 mM
Klebsiella aerogenes
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.16
-
NAD+
at pH 11.0 and 25°C
Klebsiella aerogenes
10.3
-
ribitol
at pH 11.0 and 25°C
Klebsiella aerogenes
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ba2+
209% activity at 5 mM
Klebsiella aerogenes
Ca2+
245% activity at 5 mM
Klebsiella aerogenes
Mg2+
162% activity at 5 mM
Klebsiella aerogenes
additional information
not affected by Mn2+ and Co2+
Klebsiella aerogenes
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
110000
-
gel filtration
Klebsiella aerogenes
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ribitol + NAD+
Klebsiella aerogenes
-
D-ribulose + NADH + H+
-
-
?
ribitol + NAD+
Klebsiella aerogenes KCTC 2190
-
D-ribulose + NADH + H+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Klebsiella aerogenes
A0A0H3FYM1
-
-
Klebsiella aerogenes KCTC 2190
A0A0H3FYM1
-
-
Purification (Commentary)
Commentary
Organism
Ni-NTA column chromatography
Klebsiella aerogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no activity with NADP+
738134
Klebsiella aerogenes
?
-
-
-
-
additional information
no activity with NADP+
738134
Klebsiella aerogenes KCTC 2190
?
-
-
-
-
ribitol + NAD+
-
738134
Klebsiella aerogenes
D-ribulose + NADH + H+
-
-
-
?
ribitol + NAD+
-
738134
Klebsiella aerogenes KCTC 2190
D-ribulose + NADH + H+
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 25800, calculated from amino acid sequence
Klebsiella aerogenes
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Klebsiella aerogenes
Temperature Range [°C]
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
50
60
the enzyme retains more than 90% of the maximum activity at 50°C, with more than 85% activity at 55°C, and more than 80% at 60°C
Klebsiella aerogenes
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
60
the enzyme shows half-lives of 25.7, 15.3, 7.5, 3.1, and 0.45 h at 40°C, 45°C, 50°C, 55°C, and 60°C, respectively
Klebsiella aerogenes
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
11
-
-
Klebsiella aerogenes
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
dependent on
Klebsiella aerogenes
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RIL cells
Klebsiella aerogenes
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
dependent on
Klebsiella aerogenes
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
Cu2+
50.5% residual activity at 5 mM
Klebsiella aerogenes
EDTA
the enzyme activity is mostly lost (about 97%) after EDTA treatment
Klebsiella aerogenes
Fe3+
27.2% residual activity at 5 mM
Klebsiella aerogenes
Hg2+
complete inhibition at 1 mM
Klebsiella aerogenes
K+
73.6% residual activity at 5 mM
Klebsiella aerogenes
Zn2+
61.9% residual activity at 5 mM
Klebsiella aerogenes
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.16
-
NAD+
at pH 11.0 and 25°C
Klebsiella aerogenes
10.3
-
ribitol
at pH 11.0 and 25°C
Klebsiella aerogenes
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ba2+
209% activity at 5 mM
Klebsiella aerogenes
Ca2+
245% activity at 5 mM
Klebsiella aerogenes
Mg2+
162% activity at 5 mM
Klebsiella aerogenes
additional information
not affected by Mn2+ and Co2+
Klebsiella aerogenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
110000
-
gel filtration
Klebsiella aerogenes
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ribitol + NAD+
Klebsiella aerogenes
-
D-ribulose + NADH + H+
-
-
?
ribitol + NAD+
Klebsiella aerogenes KCTC 2190
-
D-ribulose + NADH + H+
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
Ni-NTA column chromatography
Klebsiella aerogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
no activity with NADP+
738134
Klebsiella aerogenes
?
-
-
-
-
additional information
no activity with NADP+
738134
Klebsiella aerogenes KCTC 2190
?
-
-
-
-
ribitol + NAD+
-
738134
Klebsiella aerogenes
D-ribulose + NADH + H+
-
-
-
?
ribitol + NAD+
-
738134
Klebsiella aerogenes KCTC 2190
D-ribulose + NADH + H+
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 25800, calculated from amino acid sequence
Klebsiella aerogenes
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
45
-
-
Klebsiella aerogenes
Temperature Range [°C] (protein specific)
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
50
60
the enzyme retains more than 90% of the maximum activity at 50°C, with more than 85% activity at 55°C, and more than 80% at 60°C
Klebsiella aerogenes
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
40
60
the enzyme shows half-lives of 25.7, 15.3, 7.5, 3.1, and 0.45 h at 40°C, 45°C, 50°C, 55°C, and 60°C, respectively
Klebsiella aerogenes
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
11
-
-
Klebsiella aerogenes
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
30.9
-
ribitol
at pH 11.0 and 25°C
Klebsiella aerogenes
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
30.9
-
ribitol
at pH 11.0 and 25°C
Klebsiella aerogenes
Other publictions for EC 1.1.1.56
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
737628
Singh
-
D-Ribulose production by a rib ...
Klebsiella aerogenes
Biochem. Eng. J.
109
189-196
2016
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
738926
Hassanin
Cloning and characterization o ...
Providencia alcalifaciens, Providencia alcalifaciens RIMD 1656011
J. Sci. Food Agric.
96
2917-2924
2016
-
-
1
-
-
-
2
2
-
6
1
2
-
2
-
-
1
-
-
-
-
-
6
1
1
1
1
1
1
1
-
1
-
-
-
-
-
1
1
-
-
-
-
2
-
2
-
6
1
2
-
-
-
1
-
-
-
-
6
1
1
1
1
1
1
1
-
-
-
-
-
-
1
1
738134
Singh
An efficient ribitol-specific ...
Klebsiella aerogenes, Klebsiella aerogenes KCTC 2190
Enzyme Microb. Technol.
72
56-64
2015
-
-
1
-
-
-
6
2
-
4
1
2
-
5
-
-
1
-
-
-
-
-
4
1
1
1
1
-
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
6
-
2
-
4
1
2
-
-
-
1
-
-
-
-
4
1
1
1
1
-
1
-
-
-
-
-
-
-
1
1
287127
Adachi
Membrane-bound sugar alcohol d ...
Gluconobacter oxydans
Biosci. Biotechnol. Biochem.
65
115-125
2001
-
-
-
1
-
-
-
6
1
-
2
-
-
3
-
-
1
-
-
-
-
-
4
1
-
1
-
-
-
2
-
2
-
-
-
-
-
-
2
1
-
-
-
-
-
6
1
-
2
-
-
-
-
1
-
-
-
-
4
1
-
1
-
-
-
2
-
-
-
-
-
-
-
-
287126
Muniruzzaman
-
Purification and characterizat ...
Pantoea agglomerans
J. Ferment. Bioeng.
79
496-498
1995
-
-
-
-
-
-
5
7
-
2
2
-
-
1
-
-
1
-
-
-
1
-
7
1
-
-
-
-
1
-
1
1
-
-
-
-
-
-
1
-
-
-
-
5
-
7
-
2
2
-
-
-
-
1
-
-
1
-
7
1
-
-
-
-
1
-
1
-
-
-
-
-
-
-
287125
Kahle
Pentitol metabolism of Rodobac ...
Rhodobacter sphaeroides
J. Gen. Microbiol.
138
1277-1281
1992
-
-
-
-
-
-
-
5
-
1
2
-
-
4
-
-
1
-
-
-
1
-
5
1
1
-
1
-
2
2
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
5
-
1
2
-
-
-
-
1
-
-
1
-
5
1
1
-
1
-
2
2
-
-
-
-
-
-
-
-
287115
Dothie
Ribitol dehydrogenase of Klebs ...
Klebsiella aerogenes
Biochem. J.
230
569-578
1985
-
-
-
-
1
3
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
287116
Loviny
Ribitol dehydrogenase of Klebs ...
Klebsiella aerogenes
Biochem. J.
230
579-585
1985
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287117
Szumiko
The possible occurrence of zin ...
Mycobacterium sp.
Acta Biochim. Pol.
31
401-408
1984
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287118
Fromm
-
D-Ribulose ...
Klebsiella aerogenes
Methods Enzym. Anal. , 3rd Ed. (Bergmeyer, H. U. , ed. )
6
432-437
1984
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
1
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
287119
Jörnvall
Extended superfamily of short ...
Klebsiella aerogenes
FEBS Lett.
165
190-196
1984
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287120
Burleigh
A comparison of wild-type and ...
Klebsiella aerogenes
Biochem. J.
143
341-352
1974
-
-
-
-
-
2
-
3
-
-
1
1
-
1
-
-
1
-
-
-
1
2
3
2
-
-
-
3
-
-
-
2
-
-
-
-
-
-
2
-
-
2
-
-
-
3
-
-
1
1
-
-
-
1
-
-
1
2
3
2
-
-
-
3
-
-
-
-
-
-
-
-
-
-
287121
Taylor
Ribitol dehydrogenase from Kle ...
Klebsiella aerogenes
Biochem. J.
141
693-700
1974
-
-
-
-
-
2
6
3
-
1
2
-
-
1
-
-
1
-
-
-
1
2
2
1
1
-
-
-
1
-
1
2
-
-
-
-
-
-
2
-
-
2
-
6
-
3
-
1
2
-
-
-
-
1
-
-
1
2
2
1
1
-
-
-
1
-
1
-
-
-
-
-
-
-
287122
Charnetzky
Ribitol catabolic pathway in K ...
Klebsiella aerogenes
J. Bacteriol.
119
162-169
1974
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
287123
Fossitt
-
Pentitol dehydrogenases of Aer ...
Klebsiella aerogenes
Methods Enzymol.
9
180-184
1966
1
-
-
1
-
-
-
-
-
1
-
-
-
1
-
-
1
-
-
-
4
-
2
-
-
-
-
-
2
-
-
2
-
-
-
1
-
-
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
4
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
287124
Nordlie
Ribitol dehydrogenase. II. Stu ...
Klebsiella aerogenes
J. Biol. Chem.
234
2523-2531
1959
-
-
-
-
-
2
1
-
-
-
-
-
-
1
-
-
1
-
-
-
1
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
2
-
-
2
-
1
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-