BRENDA - Enzyme Database show
show all sequences of 1.1.1.54

An enone reductase from Nicotiana tabacum: cDNA cloning, expression in Escherichia coli, and reduction of enones with the recombinant proteins

Matsushima, A.; Sato, Y.; Otsuka, M.; Watanabe, T.; Yamamoto, H.; Hirata, T.; Bioorg. Chem. 36, 23-28 (2008)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
GST-fusion protein expressed in Escherichia coli BL21
Nicotiana tabacum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
(R)-pulegone
native enzyme, pH 7.0, 35C
Nicotiana tabacum
1.4
-
(R)-pulegone
recombinant enzyme, pH 7.0, 35C
Nicotiana tabacum
8.3
-
(S)-pulegone
recombinant enzyme, pH 7.0, 35C
Nicotiana tabacum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36300
-
2 * 36300, SDS-PAGE, native mass by gel filtration
Nicotiana tabacum
38482
-
2 * 38482, recombinant protein, MALDI TOF-MS, native mass by gel filtration
Nicotiana tabacum
38487
-
2 * 38487, recombinant protein, calculated from the deduced amino acid sequence, native mass by gel filtration
Nicotiana tabacum
74000
-
gel filtration
Nicotiana tabacum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nicotiana tabacum
Q9SLN8
-
-
Purification (Commentary)
Commentary
Organism
native enzyme, recombinant enzyme purified using GST-tag
Nicotiana tabacum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-pulegone + NADP+
recombinant enzyme
685505
Nicotiana tabacum
(1R,4R)-isomenthone + (1R,4S)-menthone + NADPH + H+
reaction is not stereospecific as with the native enzyme
-
-
?
(R)-pulegone + NADP+
native enzyme
685505
Nicotiana tabacum
(1R,4R)-isomenthone + NADPH + H+
-
-
-
?
(S)-pulegone + NADP+
recombinant enzyme
685505
Nicotiana tabacum
(1S,4S)-isomenthone + (1S,4R)-menthone + NADPH + H+
-
-
-
?
allyl alcohol + NADP+
-
685505
Nicotiana tabacum
acrolein + NADPH + H+
-
-
-
?
additional information
(R)-carvone, (S)-carvone, (1S,5S)-verbenone, (1R,5R)-verbenone, oxalacetic acid, and (2S,4S)-carveol do not serve as substrate
685505
Nicotiana tabacum
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
dimer
2 * 36300, SDS-PAGE, native mass by gel filtration; 2 * 38482, recombinant protein, MALDI TOF-MS, native mass by gel filtration; 2 * 38487, recombinant protein, calculated from the deduced amino acid sequence, native mass by gel filtration
Nicotiana tabacum
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
35
-
assay temperature
Nicotiana tabacum
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.8
-
(S)-pulegone
recombinant enzyme, pH 7.0, 35C
Nicotiana tabacum
3.3
-
(R)-pulegone
recombinant enzyme, pH 7.0, 35C
Nicotiana tabacum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay pH
Nicotiana tabacum
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Nicotiana tabacum
Cloned(Commentary) (protein specific)
Commentary
Organism
GST-fusion protein expressed in Escherichia coli BL21
Nicotiana tabacum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Nicotiana tabacum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.025
-
(R)-pulegone
native enzyme, pH 7.0, 35C
Nicotiana tabacum
1.4
-
(R)-pulegone
recombinant enzyme, pH 7.0, 35C
Nicotiana tabacum
8.3
-
(S)-pulegone
recombinant enzyme, pH 7.0, 35C
Nicotiana tabacum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
36300
-
2 * 36300, SDS-PAGE, native mass by gel filtration
Nicotiana tabacum
38482
-
2 * 38482, recombinant protein, MALDI TOF-MS, native mass by gel filtration
Nicotiana tabacum
38487
-
2 * 38487, recombinant protein, calculated from the deduced amino acid sequence, native mass by gel filtration
Nicotiana tabacum
74000
-
gel filtration
Nicotiana tabacum
Purification (Commentary) (protein specific)
Commentary
Organism
native enzyme, recombinant enzyme purified using GST-tag
Nicotiana tabacum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(R)-pulegone + NADP+
recombinant enzyme
685505
Nicotiana tabacum
(1R,4R)-isomenthone + (1R,4S)-menthone + NADPH + H+
reaction is not stereospecific as with the native enzyme
-
-
?
(R)-pulegone + NADP+
native enzyme
685505
Nicotiana tabacum
(1R,4R)-isomenthone + NADPH + H+
-
-
-
?
(S)-pulegone + NADP+
recombinant enzyme
685505
Nicotiana tabacum
(1S,4S)-isomenthone + (1S,4R)-menthone + NADPH + H+
-
-
-
?
allyl alcohol + NADP+
-
685505
Nicotiana tabacum
acrolein + NADPH + H+
-
-
-
?
additional information
(R)-carvone, (S)-carvone, (1S,5S)-verbenone, (1R,5R)-verbenone, oxalacetic acid, and (2S,4S)-carveol do not serve as substrate
685505
Nicotiana tabacum
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 36300, SDS-PAGE, native mass by gel filtration; 2 * 38482, recombinant protein, MALDI TOF-MS, native mass by gel filtration; 2 * 38487, recombinant protein, calculated from the deduced amino acid sequence, native mass by gel filtration
Nicotiana tabacum
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
35
-
assay temperature
Nicotiana tabacum
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
2.8
-
(S)-pulegone
recombinant enzyme, pH 7.0, 35C
Nicotiana tabacum
3.3
-
(R)-pulegone
recombinant enzyme, pH 7.0, 35C
Nicotiana tabacum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay pH
Nicotiana tabacum
Other publictions for EC 1.1.1.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724009
Zhu
Coproduction of acetaldehyde a ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
77
6441-6450
2011
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1
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1
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1
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1
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1
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725090
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Fusarium oxysporum Adh1 has du ...
Fusarium oxysporum
Fungal Genet. Biol.
48
886-895
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-
-
-
-
-
-
-
-
-
-
-
-
1
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-
1
1
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-
685505
Matsushima
An enone reductase from Nicoti ...
Nicotiana tabacum
Bioorg. Chem.
36
23-28
2008
-
-
1
-
-
-
-
3
-
-
4
-
-
3
-
-
1
-
-
-
-
-
5
1
1
-
-
2
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
4
-
-
-
-
1
-
-
-
-
5
1
1
-
-
2
1
-
-
-
-
-
-
-
-
-
287113
Hirata
A 38 kDa allylic alcohol dehyd ...
Nicotiana tabacum
Phytochemistry
55
297-303
2000
-
-
1
-
-
-
-
-
-
-
2
1
-
3
-
-
1
-
-
-
1
-
4
1
-
-
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
1
-
4
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
287114
Malone
Characterization of a Pseudomo ...
Pseudomonas putida, Pseudomonas putida MB-1
Appl. Environ. Microbiol.
65
2622-2630
1999
-
-
-
-
-
-
4
12
-
1
2
2
-
2
-
-
1
1
-
-
12
1
10
1
-
-
-
-
2
-
1
1
-
-
-
-
-
-
1
-
-
-
-
4
-
12
-
1
2
2
-
-
-
1
-
-
12
1
10
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
287112
Otsuka
-
Triphosphopyridine nucleotide- ...
Escherichia coli
J. Gen. Appl. Microbiol.
4
211-215
1958
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5
2
-
1
-
1
-
1
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1
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1
-
4
-
-
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-
-
1
-
-
2
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-
-
2
-
-
-
-
5
-
2
-
1
-
1
-
-
-
1
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
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