BRENDA - Enzyme Database show
show all sequences of 1.1.1.54

Characterization of a Pseudomonas putida allylic alcohol dehydrogenase induced by growth on 2-methyl-3-buten-2-ol

Malone, V.F.; Chastian, A.J.; Ohlsson, J.T.; Poneleit, L.S.; Nemecek-Marshall, M.; Fall, R.; Appl. Environ. Microbiol. 65, 2622-2630 (1999)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
3-methyl-2-buten-1-al
product inhibition, therefore the product must be removed via reaction with hydrazine during the enzyme assay
Pseudomonas putida
dithiothreitol
-
Pseudomonas putida
EDTA
only at concentration of 100 mM 50% reduction
Pseudomonas putida
N-ethylmaleimide
-
Pseudomonas putida
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.007
-
3-chloro-2-buten-1-ol
-
Pseudomonas putida
0.007
-
benzyl alcohol
-
Pseudomonas putida
0.011
-
3-aminobenzyl alcohol
-
Pseudomonas putida
0.012
-
(R)-(+)-perillyl alcohol
-
Pseudomonas putida
0.036
-
cinnamyl alcohol
-
Pseudomonas putida
0.04
-
3-methyl-2-buten-1-ol
-
Pseudomonas putida
0.044
-
2-Buten-1-ol
-
Pseudomonas putida
0.064
-
NAD+
with 3-methyl-2-buten-1-ol
Pseudomonas putida
0.118
-
NAD+
with benzyl alcohol
Pseudomonas putida
0.27
-
3-methyl-1-butanol
-
Pseudomonas putida
1.1
-
1-butanol
-
Pseudomonas putida
1.7
-
3-methyl-3-buten-1-ol
-
Pseudomonas putida
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
is not sensitive against metal ion chelators
Pseudomonas putida
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37700
-
4 * 37700, SDS-PAGE
Pseudomonas putida
151000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-methyl-2-buten-1-ol + NAD+
Pseudomonas putida
can use substrate as sole carbon source
3-methyl-2-buten-1-al + NADH
-
Pseudomonas putida
?
3-methyl-2-buten-1-ol + NAD+
Pseudomonas putida MB-1
can use substrate as sole carbon source
3-methyl-2-buten-1-al + NADH
-
Pseudomonas putida MB-1
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas putida
-
strain MB-1
-
Pseudomonas putida MB-1
-
strain MB-1
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas putida
Reaction
Reaction
Commentary
Organism
allyl alcohol + NADP+ = acrolein + NADPH + H+
3-methyl-2-buten-1-ol-dependent inducible activity
Pseudomonas putida
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.05
-
purified enzyme, substrate 1-butanol
Pseudomonas putida
0.08
-
purified enzyme, substrate 3-methyl-3-buten-1-ol
Pseudomonas putida
0.2
-
purified enzyme, substrate 3-methyl-1-butanol
Pseudomonas putida
3.7
-
purified enzyme, substrate cinnamyl alcohol
Pseudomonas putida
4.6
-
purified enzyme, substrate 2-buten-1-ol
Pseudomonas putida
7.7
-
purified enzyme, substrate 3-methyl-2-buten-1-ol
Pseudomonas putida
8.8
-
purified enzyme, substrate (R)-(+)-perillyl alcohol
Pseudomonas putida
16
-
purified enzyme, substrate 3-chlor-2-buten-1-ol
Pseudomonas putida
19.3
-
purified enzyme, in glycine buffer pH 9.4
Pseudomonas putida
22
-
purified enzyme, substrate benzyl alcohol
Pseudomonas putida
38
-
purified enzyme, substrate aromatic 3-aminobenzyl alcohol
Pseudomonas putida
38.8
-
purified enzyme, in bis-Tris-propane-hydrazine buffer pH 9.4
Pseudomonas putida
Storage Stability
Storage Stability
Organism
-70°C, 50-65% ethylene glycol (v/v), frozen in liquid nitrogen, stable for several month
Pseudomonas putida
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-buten-2-ol + NADPH
-
287114
Pseudomonas putida
but-2-enal
-
-
-
?
2-buten-2-ol + NADPH
-
287114
Pseudomonas putida MB-1
but-2-enal
-
-
-
?
3-chloro-2-buten-1-ol + NADP+
-
287114
Pseudomonas putida
3-chloro-2-butenaldehyde + NADPH
-
-
-
?
3-chloro-2-buten-1-ol + NADP+
-
287114
Pseudomonas putida MB-1
3-chloro-2-butenaldehyde + NADPH
-
-
-
?
3-methyl-2-buten-1-ol + NAD+
-
287114
Pseudomonas putida
3-methyl-2-buten-1-al + NADH
-
287114
Pseudomonas putida
?
3-methyl-2-buten-1-ol + NAD+
can use substrate as sole carbon source
287114
Pseudomonas putida
3-methyl-2-buten-1-al + NADH
-
287114
Pseudomonas putida
?
3-methyl-2-buten-1-ol + NAD+
-
287114
Pseudomonas putida MB-1
3-methyl-2-buten-1-al + NADH
-
287114
Pseudomonas putida MB-1
?
3-methyl-2-buten-1-ol + NAD+
can use substrate as sole carbon source
287114
Pseudomonas putida MB-1
3-methyl-2-buten-1-al + NADH
-
287114
Pseudomonas putida MB-1
?
cinnamyl alcohol + NADP+
-
287114
Pseudomonas putida
cinnamyl aldehyde + NADPH
-
-
-
?
cinnamyl alcohol + NADP+
-
287114
Pseudomonas putida MB-1
cinnamyl aldehyde + NADPH
-
-
-
?
Subunits
Subunits
Commentary
Organism
tetramer
4 * 37700, SDS-PAGE
Pseudomonas putida
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4
-
for reduction
Pseudomonas putida
10
-
for oxidation
Pseudomonas putida
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
9.4
-
stability at pH 9.4, 25°C much higher than at reactions optimal pH of 10.0
Pseudomonas putida
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
exhibits less than 5% of the activity with NADP+
Pseudomonas putida
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
exhibits less than 5% of the activity with NADP+
Pseudomonas putida
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-methyl-2-buten-1-al
product inhibition, therefore the product must be removed via reaction with hydrazine during the enzyme assay
Pseudomonas putida
dithiothreitol
-
Pseudomonas putida
EDTA
only at concentration of 100 mM 50% reduction
Pseudomonas putida
N-ethylmaleimide
-
Pseudomonas putida
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.007
-
3-chloro-2-buten-1-ol
-
Pseudomonas putida
0.007
-
benzyl alcohol
-
Pseudomonas putida
0.011
-
3-aminobenzyl alcohol
-
Pseudomonas putida
0.012
-
(R)-(+)-perillyl alcohol
-
Pseudomonas putida
0.036
-
cinnamyl alcohol
-
Pseudomonas putida
0.04
-
3-methyl-2-buten-1-ol
-
Pseudomonas putida
0.044
-
2-Buten-1-ol
-
Pseudomonas putida
0.064
-
NAD+
with 3-methyl-2-buten-1-ol
Pseudomonas putida
0.118
-
NAD+
with benzyl alcohol
Pseudomonas putida
0.27
-
3-methyl-1-butanol
-
Pseudomonas putida
1.1
-
1-butanol
-
Pseudomonas putida
1.7
-
3-methyl-3-buten-1-ol
-
Pseudomonas putida
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
is not sensitive against metal ion chelators
Pseudomonas putida
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37700
-
4 * 37700, SDS-PAGE
Pseudomonas putida
151000
-
gel filtration
Pseudomonas putida
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-methyl-2-buten-1-ol + NAD+
Pseudomonas putida
can use substrate as sole carbon source
3-methyl-2-buten-1-al + NADH
-
Pseudomonas putida
?
3-methyl-2-buten-1-ol + NAD+
Pseudomonas putida MB-1
can use substrate as sole carbon source
3-methyl-2-buten-1-al + NADH
-
Pseudomonas putida MB-1
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas putida
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.05
-
purified enzyme, substrate 1-butanol
Pseudomonas putida
0.08
-
purified enzyme, substrate 3-methyl-3-buten-1-ol
Pseudomonas putida
0.2
-
purified enzyme, substrate 3-methyl-1-butanol
Pseudomonas putida
3.7
-
purified enzyme, substrate cinnamyl alcohol
Pseudomonas putida
4.6
-
purified enzyme, substrate 2-buten-1-ol
Pseudomonas putida
7.7
-
purified enzyme, substrate 3-methyl-2-buten-1-ol
Pseudomonas putida
8.8
-
purified enzyme, substrate (R)-(+)-perillyl alcohol
Pseudomonas putida
16
-
purified enzyme, substrate 3-chlor-2-buten-1-ol
Pseudomonas putida
19.3
-
purified enzyme, in glycine buffer pH 9.4
Pseudomonas putida
22
-
purified enzyme, substrate benzyl alcohol
Pseudomonas putida
38
-
purified enzyme, substrate aromatic 3-aminobenzyl alcohol
Pseudomonas putida
38.8
-
purified enzyme, in bis-Tris-propane-hydrazine buffer pH 9.4
Pseudomonas putida
Storage Stability (protein specific)
Storage Stability
Organism
-70°C, 50-65% ethylene glycol (v/v), frozen in liquid nitrogen, stable for several month
Pseudomonas putida
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-buten-2-ol + NADPH
-
287114
Pseudomonas putida
but-2-enal
-
-
-
?
2-buten-2-ol + NADPH
-
287114
Pseudomonas putida MB-1
but-2-enal
-
-
-
?
3-chloro-2-buten-1-ol + NADP+
-
287114
Pseudomonas putida
3-chloro-2-butenaldehyde + NADPH
-
-
-
?
3-chloro-2-buten-1-ol + NADP+
-
287114
Pseudomonas putida MB-1
3-chloro-2-butenaldehyde + NADPH
-
-
-
?
3-methyl-2-buten-1-ol + NAD+
-
287114
Pseudomonas putida
3-methyl-2-buten-1-al + NADH
-
287114
Pseudomonas putida
?
3-methyl-2-buten-1-ol + NAD+
can use substrate as sole carbon source
287114
Pseudomonas putida
3-methyl-2-buten-1-al + NADH
-
287114
Pseudomonas putida
?
3-methyl-2-buten-1-ol + NAD+
-
287114
Pseudomonas putida MB-1
3-methyl-2-buten-1-al + NADH
-
287114
Pseudomonas putida MB-1
?
3-methyl-2-buten-1-ol + NAD+
can use substrate as sole carbon source
287114
Pseudomonas putida MB-1
3-methyl-2-buten-1-al + NADH
-
287114
Pseudomonas putida MB-1
?
cinnamyl alcohol + NADP+
-
287114
Pseudomonas putida
cinnamyl aldehyde + NADPH
-
-
-
?
cinnamyl alcohol + NADP+
-
287114
Pseudomonas putida MB-1
cinnamyl aldehyde + NADPH
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
tetramer
4 * 37700, SDS-PAGE
Pseudomonas putida
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5.4
-
for reduction
Pseudomonas putida
10
-
for oxidation
Pseudomonas putida
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
9.4
-
stability at pH 9.4, 25°C much higher than at reactions optimal pH of 10.0
Pseudomonas putida
Other publictions for EC 1.1.1.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724009
Zhu
Coproduction of acetaldehyde a ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
77
6441-6450
2011
-
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1
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1
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1
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1
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1
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-
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-
725090
Corrales Escobosa
Fusarium oxysporum Adh1 has du ...
Fusarium oxysporum
Fungal Genet. Biol.
48
886-895
2011
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-
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1
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1
1
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685505
Matsushima
An enone reductase from Nicoti ...
Nicotiana tabacum
Bioorg. Chem.
36
23-28
2008
-
-
1
-
-
-
-
3
-
-
4
-
-
3
-
-
1
-
-
-
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-
5
1
1
-
-
2
1
-
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1
-
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1
1
-
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-
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-
3
-
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4
-
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-
1
-
-
-
-
5
1
1
-
-
2
1
-
-
-
-
-
-
-
-
-
287113
Hirata
A 38 kDa allylic alcohol dehyd ...
Nicotiana tabacum
Phytochemistry
55
297-303
2000
-
-
1
-
-
-
-
-
-
-
2
1
-
3
-
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1
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-
-
1
-
4
1
-
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-
2
-
-
2
-
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-
1
2
-
-
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-
-
-
-
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-
2
1
-
-
-
1
-
-
1
-
4
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
287114
Malone
Characterization of a Pseudomo ...
Pseudomonas putida, Pseudomonas putida MB-1
Appl. Environ. Microbiol.
65
2622-2630
1999
-
-
-
-
-
-
4
12
-
1
2
2
-
2
-
-
1
1
-
-
12
1
10
1
-
-
-
-
2
-
1
1
-
-
-
-
-
-
1
-
-
-
-
4
-
12
-
1
2
2
-
-
-
1
-
-
12
1
10
1
-
-
-
-
2
-
1
-
-
-
-
-
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-
287112
Otsuka
-
Triphosphopyridine nucleotide- ...
Escherichia coli
J. Gen. Appl. Microbiol.
4
211-215
1958
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5
2
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1
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1
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1
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1
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1
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4
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1
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2
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2
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5
-
2
-
1
-
1
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1
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1
-
4
-
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1
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