BRENDA - Enzyme Database show
show all sequences of 1.1.1.54

A 38 kDa allylic alcohol dehydrogenase from the cultured cells of Nicotiana tabacum

Hirata, T.; Tamura, Y.; Yokobatake, N.; Shimoda, K.; Ashida, Y.; Phytochemistry 55, 297-303 (2000)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
sequence homology with other NADPH-dependent oxidoreductases from plants
Nicotiana tabacum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
2 * 37000, SDS-PAGE and amino acid sequence analysis
Nicotiana tabacum
74000
-
gel filtration
Nicotiana tabacum
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
allyl alcohol + NADP+
Nicotiana tabacum
-
acrolein + NADPH
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Nicotiana tabacum
-
-
-
Purification (Commentary)
Commentary
Organism
-
Nicotiana tabacum
Specific Activity [micromol/min/mg]
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.11
-
-
Nicotiana tabacum
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S,4S)-carveol + NADP+
-
287113
Nicotiana tabacum
(S)-carvone + NADPH
-
287113
Nicotiana tabacum
r
(S)-carvone + NADPH
-
287113
Nicotiana tabacum
(2S,4S)-carveol + NADP+
-
287113
Nicotiana tabacum
r
allyl alcohol + NADP+
-
287113
Nicotiana tabacum
acrolein + NADPH
-
-
-
r
allyl alcohol + NADP+
specific for the (S)-isomer of secondary allylic alcohols, several stereomers tested for activity in both reaction directions
287113
Nicotiana tabacum
acrolein + NADPH
-
-
-
r
Subunits
Subunits
Commentary
Organism
dimer
2 * 37000, SDS-PAGE and amino acid sequence analysis
Nicotiana tabacum
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
reduction of carbonyl group of (S)-carvone
Nicotiana tabacum
8
-
dehydrogenation of (S)-carveol
Nicotiana tabacum
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)H
-
Nicotiana tabacum
NADP+
activity 4times greater than with NAD+
Nicotiana tabacum
Cloned(Commentary) (protein specific)
Commentary
Organism
sequence homology with other NADPH-dependent oxidoreductases from plants
Nicotiana tabacum
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)H
-
Nicotiana tabacum
NADP+
activity 4times greater than with NAD+
Nicotiana tabacum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
37000
-
2 * 37000, SDS-PAGE and amino acid sequence analysis
Nicotiana tabacum
74000
-
gel filtration
Nicotiana tabacum
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
allyl alcohol + NADP+
Nicotiana tabacum
-
acrolein + NADPH
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
-
Nicotiana tabacum
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
0.11
-
-
Nicotiana tabacum
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
(2S,4S)-carveol + NADP+
-
287113
Nicotiana tabacum
(S)-carvone + NADPH
-
287113
Nicotiana tabacum
r
(S)-carvone + NADPH
-
287113
Nicotiana tabacum
(2S,4S)-carveol + NADP+
-
287113
Nicotiana tabacum
r
allyl alcohol + NADP+
-
287113
Nicotiana tabacum
acrolein + NADPH
-
-
-
r
allyl alcohol + NADP+
specific for the (S)-isomer of secondary allylic alcohols, several stereomers tested for activity in both reaction directions
287113
Nicotiana tabacum
acrolein + NADPH
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 37000, SDS-PAGE and amino acid sequence analysis
Nicotiana tabacum
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
reduction of carbonyl group of (S)-carvone
Nicotiana tabacum
8
-
dehydrogenation of (S)-carveol
Nicotiana tabacum
Other publictions for EC 1.1.1.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [░C]
Temperature Range [░C]
Temperature Stability [░C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [░C] (protein specific)
Temperature Range [░C] (protein specific)
Temperature Stability [░C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724009
Zhu
Coproduction of acetaldehyde a ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
77
6441-6450
2011
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-
1
-
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-
1
-
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1
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-
1
-
-
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-
-
-
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-
-
-
-
-
-
-
-
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-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725090
Corrales Escobosa
Fusarium oxysporum Adh1 has du ...
Fusarium oxysporum
Fungal Genet. Biol.
48
886-895
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
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1
1
-
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-
685505
Matsushima
An enone reductase from Nicoti ...
Nicotiana tabacum
Bioorg. Chem.
36
23-28
2008
-
-
1
-
-
-
-
3
-
-
4
-
-
3
-
-
1
-
-
-
-
-
5
1
1
-
-
2
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
4
-
-
-
-
1
-
-
-
-
5
1
1
-
-
2
1
-
-
-
-
-
-
-
-
-
287113
Hirata
A 38 kDa allylic alcohol dehyd ...
Nicotiana tabacum
Phytochemistry
55
297-303
2000
-
-
1
-
-
-
-
-
-
-
2
1
-
3
-
-
1
-
-
-
1
-
4
1
-
-
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
1
-
4
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
287114
Malone
Characterization of a Pseudomo ...
Pseudomonas putida, Pseudomonas putida MB-1
Appl. Environ. Microbiol.
65
2622-2630
1999
-
-
-
-
-
-
4
12
-
1
2
2
-
2
-
-
1
1
-
-
12
1
10
1
-
-
-
-
2
-
1
1
-
-
-
-
-
-
1
-
-
-
-
4
-
12
-
1
2
2
-
-
-
1
-
-
12
1
10
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
287112
Otsuka
-
Triphosphopyridine nucleotide- ...
Escherichia coli
J. Gen. Appl. Microbiol.
4
211-215
1958
-
-
-
-
-
-
5
2
-
1
-
1
-
1
-
-
1
-
-
-
1
-
4
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
5
-
2
-
1
-
1
-
-
-
1
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-