BRENDA - Enzyme Database show
show all sequences of 1.1.1.54

Triphosphopyridine nucleotide-allyl and -ethyl alcohol dehydrogenases from Escherichia coli (preliminary report)

Otsuka, K.; J. Gen. Appl. Microbiol. 4, 211-215 (1958)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
cyanide
-
Escherichia coli
dipyridyl
-
Escherichia coli
EDTA
-
Escherichia coli
additional information
not: monoiodoacetate
Escherichia coli
p-chloromercuribenzoate
can be overcome by addition of gluthathione
Escherichia coli
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
NADP+
-
Escherichia coli
5
-
allyl-alcohol
-
Escherichia coli
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
is sensitive against metal ion chelators
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
allyl alcohol + NADP+
Escherichia coli
-
acrolein + NADPH
-
-
r
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Escherichia coli
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Escherichia coli
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
about 70,1 unit of enzyme activity defined as amount of enzyme which causes a change in optical density of 0.001 per minute
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-butylene glycol + NADP+
-
287112
Escherichia coli
?
-
-
-
r
allyl alcohol + NADP+
-
287112
Escherichia coli
acrolein + NADPH
-
-
-
r
allyl alcohol + NADP+
-
287112
Escherichia coli
acrolein + NADPH
-
287112
Escherichia coli
r
saturated primary alcohols + NADP+
i.e. ethyl alcohol, n-propyl alcohol, n-butyl acohol, isobutyl alcohol, propylene glycol
287112
Escherichia coli
?
-
-
-
r
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
in Tris-buffer
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
NAD(P)H
-
Escherichia coli
NADP+
specific for
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD(P)H
-
Escherichia coli
NADP+
specific for
Escherichia coli
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
cyanide
-
Escherichia coli
dipyridyl
-
Escherichia coli
EDTA
-
Escherichia coli
additional information
not: monoiodoacetate
Escherichia coli
p-chloromercuribenzoate
can be overcome by addition of gluthathione
Escherichia coli
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.04
-
NADP+
-
Escherichia coli
5
-
allyl-alcohol
-
Escherichia coli
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
is sensitive against metal ion chelators
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
allyl alcohol + NADP+
Escherichia coli
-
acrolein + NADPH
-
-
r
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
additional information
-
about 70,1 unit of enzyme activity defined as amount of enzyme which causes a change in optical density of 0.001 per minute
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-butylene glycol + NADP+
-
287112
Escherichia coli
?
-
-
-
r
allyl alcohol + NADP+
-
287112
Escherichia coli
acrolein + NADPH
-
-
-
r
allyl alcohol + NADP+
-
287112
Escherichia coli
acrolein + NADPH
-
287112
Escherichia coli
r
saturated primary alcohols + NADP+
i.e. ethyl alcohol, n-propyl alcohol, n-butyl acohol, isobutyl alcohol, propylene glycol
287112
Escherichia coli
?
-
-
-
r
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.7
-
in Tris-buffer
Escherichia coli
Other publictions for EC 1.1.1.54
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724009
Zhu
Coproduction of acetaldehyde a ...
Saccharomyces cerevisiae
Appl. Environ. Microbiol.
77
6441-6450
2011
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
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-
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-
-
-
-
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
725090
Corrales Escobosa
Fusarium oxysporum Adh1 has du ...
Fusarium oxysporum
Fungal Genet. Biol.
48
886-895
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
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-
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-
-
-
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-
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-
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-
-
-
-
-
-
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
685505
Matsushima
An enone reductase from Nicoti ...
Nicotiana tabacum
Bioorg. Chem.
36
23-28
2008
-
-
1
-
-
-
-
3
-
-
4
-
-
3
-
-
1
-
-
-
-
-
5
1
1
-
-
2
1
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
3
-
-
4
-
-
-
-
1
-
-
-
-
5
1
1
-
-
2
1
-
-
-
-
-
-
-
-
-
287113
Hirata
A 38 kDa allylic alcohol dehyd ...
Nicotiana tabacum
Phytochemistry
55
297-303
2000
-
-
1
-
-
-
-
-
-
-
2
1
-
3
-
-
1
-
-
-
1
-
4
1
-
-
-
-
2
-
-
2
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
2
1
-
-
-
1
-
-
1
-
4
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
287114
Malone
Characterization of a Pseudomo ...
Pseudomonas putida, Pseudomonas putida MB-1
Appl. Environ. Microbiol.
65
2622-2630
1999
-
-
-
-
-
-
4
12
-
1
2
2
-
2
-
-
1
1
-
-
12
1
10
1
-
-
-
-
2
-
1
1
-
-
-
-
-
-
1
-
-
-
-
4
-
12
-
1
2
2
-
-
-
1
-
-
12
1
10
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
287112
Otsuka
-
Triphosphopyridine nucleotide- ...
Escherichia coli
J. Gen. Appl. Microbiol.
4
211-215
1958
-
-
-
-
-
-
5
2
-
1
-
1
-
1
-
-
1
-
-
-
1
-
4
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
5
-
2
-
1
-
1
-
-
-
1
-
-
1
-
4
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-