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Literature summary for 1.1.1.53 extracted from

  • Zhang, B.; Zhu, D.; Hu, X.; Zhou, M.; Shang, P.; Lin, S.
    Human 3-alpha hydroxysteroid dehydrogenase type 3 (3alpha-HSD3): The V54L mutation restricting the steroid alternative binding and enhancing the 20alpha-HSD activity (2014), J. Steroid Biochem. Mol. Biol., 141, 135-143.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with NADP+ and progesterone, sitting drop vapor diffusion method, using 100 mM sodiumcacodylate buffer (pH 6.0), 200 mM ammonium sulfate, 24-26% (w/v) polyethylene glycol 3350 Homo sapiens

Protein Variants

Protein Variants Comment Organism
V54L the mutation restricts the steroid alternative binding and enhances the 20alpha-hydroxysteroid dehydrogenase activity of the enzyme Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00084
-
progesterone wild type enzyme, at pH 7.4 and 37°C Homo sapiens
0.00213
-
progesterone mutant enzyme, at pH 7.4 and 37°C Homo sapiens
0.00218
-
5alpha-dihydrotestosterone wild type enzyme, at pH 7.4 and 37°C Homo sapiens
0.07103
-
5alpha-dihydrotestosterone mutant enzyme, at pH 7.4 and 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione agarose column chromatography and Q-Sepharose column chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5alpha-dihydrotestosterone + NADP+
-
Homo sapiens 5alpha-androstane-3,17-dione + NADPH + H+
-
?
progesterone + NADP+
-
Homo sapiens 20beta-hydroxy-pregn-4-ene-3-one + NADPH + H+
-
?

Synonyms

Synonyms Comment Organism
3-alpha hydroxysteroid dehydrogenase type 3
-
Homo sapiens
3alpha-HSD3
-
Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.004
-
progesterone wild type enzyme, at pH 7.4 and 37°C Homo sapiens
0.039
-
progesterone mutant enzyme, at pH 7.4 and 37°C Homo sapiens
0.061
-
5alpha-dihydrotestosterone wild type enzyme, at pH 7.4 and 37°C Homo sapiens
0.115
-
5alpha-dihydrotestosterone mutant enzyme, at pH 7.4 and 37°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
100
-
5alpha-dihydrotestosterone mutant enzyme, at pH 7.4 and 37°C Homo sapiens
250
-
progesterone wild type enzyme, at pH 7.4 and 37°C Homo sapiens
1100
-
progesterone mutant enzyme, at pH 7.4 and 37°C Homo sapiens
1680
-
5alpha-dihydrotestosterone wild type enzyme, at pH 7.4 and 37°C Homo sapiens