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Literature summary for 1.1.1.51 extracted from
- Characterization of hamster NAD+-dependent 3(17)beta-hydroxysteroid dehydrogenase belonging to the aldo-keto reductase 1C subfamily (2015), J. Biochem., 158, 425-434.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene AKR1C35, cloned from liver, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS | Mesocricetus auratus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| C310F | site-directed mutagenesis, the mutation, in analogy to enzyme AKR1C34 sequence, does not affect the enzyme activity of AKR1C35 | Mesocricetus auratus |
| V54A | site-directed mutagenesis, the mutation, in analogy to enzyme AKR1C34 sequence, impairs the 3beta-hydroxysteroid dehydrogenase activity of AKR1C35 | Mesocricetus auratus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 7-hydroxyflavone | - |
Mesocricetus auratus |  |
| chrysin | - |
Mesocricetus auratus | |
| galangin | - |
Mesocricetus auratus | |
| genistein | - |
Mesocricetus auratus | |
| Hexestrol | - |
Mesocricetus auratus | |
| hinokitiol | - |
Mesocricetus auratus | |
| lithocholic acid | - |
Mesocricetus auratus | |
| Phenolphthalein | - |
Mesocricetus auratus | |
| quercetin | - |
Mesocricetus auratus | |
| zearalenone | - |
Mesocricetus auratus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Michaelis-Menten kinetics | Mesocricetus auratus | |
| 0.0004 | - |
17beta-estradiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0009 | - |
3beta-hydroxyprogesterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.001 | - |
geranylgeraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.001 | - |
beta-ionol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0012 | - |
farnesol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0012 | - |
(S)-1-indanol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0015 | - |
(S)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0025 | - |
5alpha-Androstan-3beta-ol-17-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0033 | - |
5-androstene-3beta,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0039 | - |
5beta-androstane-3alpha,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0039 | - |
5alpha-pregnan-3beta-ol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0041 | - |
geraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0043 | - |
dehydroepiandrosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0068 | - |
5beta-Pregnan-3beta-ol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0076 | - |
5alpha-dihydrotestosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0081 | - |
testosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0081 | - |
4-androstene-3alpha,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0097 | - |
cis-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.011 | - |
nerol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.011 | - |
trans-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.013 | - |
5beta-dihydrotestosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.014 | - |
alpha-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.015 | - |
5beta-androstane-3beta,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.015 | - |
5beta-pregnan-3beta,21-diol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.017 | - |
NADH | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus | |
| 0.023 | - |
1-Acenaphthenol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.023 | - |
NAD+ | pH 7.4, 25°C, with substrate S-tetralol | Mesocricetus auratus | |
| 0.026 | - |
2-Cyclohexen-1-ol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.028 | - |
(R)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.04 | - |
beta-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.046 | - |
5beta-androstan-3beta-ol-17-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.074 | - |
Cyclohexanol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 2.4 | - |
NADP+ | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Mesocricetus auratus | 5829 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 37000, recombinant His-tagged enzyme, SDS-PAGE | Mesocricetus auratus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mesocricetus auratus | A0A097ZMZ1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, ultrafiltration, and dialysis | Mesocricetus auratus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal gland | in female hamsters | Mesocricetus auratus | - |
| brain | in female hamsters | Mesocricetus auratus | - |
| colon | in female and male hamsters | Mesocricetus auratus | - |
| heart | in female hamsters | Mesocricetus auratus | - |
| kidney | in female hamsters | Mesocricetus auratus | - |
| liver | - |
Mesocricetus auratus | - |
| liver | in female and male hamsters | Mesocricetus auratus | - |
| lung | in female hamsters | Mesocricetus auratus | - |
| additional information | mRNA for AKR1C34 is expressed liver-specific in male hamsters and ubiquitously in female hamsters | Mesocricetus auratus | - |
| ovary | - |
Mesocricetus auratus | - |
| small intestine | in female hamsters | Mesocricetus auratus | - |
| stomach | in female hamsters | Mesocricetus auratus | - |
| testis | in female hamsters | Mesocricetus auratus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-1,2,3,4-tetrahydro-1-naphthol + NAD+ | i.e. R-tetralol | Mesocricetus auratus | 3,4-dihydronaphthalen-1(2H)-one + NADH + H+ | - |
? | |
| (S)-1,2,3,4-tetrahydro-1-naphthol + NAD+ | i.e. S-tetralol | Mesocricetus auratus | 3,4-dihydronaphthalen-1(2H)-one + NADH + H+ | - |
? | |
| (S)-1,2,3,4-tetrahydro-1-naphthol + NADP+ | i.e. S-tetralol | Mesocricetus auratus | 3,4-dihydronaphthalen-1(2H)-one + NADPH + H+ | - |
? | |
| (S)-1-indanol + NAD+ | - |
Mesocricetus auratus | 2,3-dihydro-1H-inden-1-one + NADH + H+ | - |
? | |
| (S)-camphorquinone + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
| (S)-camphorquinone + NADPH + H+ | - |
Mesocricetus auratus | ? + NADP+ | - |
? | |
| 1-acenaphthenol + NAD+ | - |
Mesocricetus auratus | acenaphthylen-1(2H)-one + NADH + H+ | - |
? | |
| 17beta-estradiol + NAD+ | - |
Mesocricetus auratus | estrone + NADH + H+ | - |
? | |
| 2-cyclohexen-1-ol + NAD+ | - |
Mesocricetus auratus | 2-cyclohexen-1-one + NADH + H+ | - |
? | |
| 3beta-hydroxyprogesterone + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
| 4-androstene-3alpha,17beta-diol + NAD+ | - |
Mesocricetus auratus | ? + NADH + H+ | - |
? | |
| 5-androstene-3beta,17beta-diol + NAD+ | - |
Mesocricetus auratus | ? + NADH + H+ | - |
? | |
| 5alpha-androstan-3alpha-ol-17-one + NAD+ | - |
Mesocricetus auratus | 5alpha-androstan-3,17-dione + NADH + H+ | - |
? | |
| 5alpha-androstan-3beta-ol-17-one + NAD+ | - |
Mesocricetus auratus | 5alpha-androstan-3,17-dione + NADH + H+ | - |
? | |
| 5alpha-androstane-3alpha,17beta-diol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
| 5alpha-dihydrotestosterone + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
| 5alpha-pregnan-3alpha-ol-20-one + NAD+ | - |
Mesocricetus auratus | 5alpha-pregnan-3,20-dione + NADH + H+ | - |
? | |
| 5alpha-pregnan-3beta-ol-20-one + NAD+ | - |
Mesocricetus auratus | 5alpha-pregnan-3,20-dione + NADH + H+ | - |
? | |
| 5beta-androstan-17beta-ol-3-one + NAD+ | - |
Mesocricetus auratus | 5beta-androstan-3,17-dione + NADH + H+ | - |
? | |
| 5beta-androstan-3alpha-ol-17-one + NAD+ | - |
Mesocricetus auratus | 5beta-androstan-3,17-dione + NADH + H+ | - |
? | |
| 5beta-androstan-3beta-ol-17-one + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
| 5beta-androstane-3alpha,17beta-diol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
| 5beta-androstane-3beta,17beta-diol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
| 5beta-dihydrotestosterone + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
| 5beta-pregnan-20alpha-ol-3-one + NAD+ | - |
Mesocricetus auratus | 5beta-pregnan-3,20-dione + NADH + H+ | - |
? | |
| 5beta-pregnan-3beta,21-diol-20-one + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
| 5beta-pregnan-3beta,21-diol-20-one + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
| 5beta-pregnan-3beta-ol-20-one + NAD+ | - |
Mesocricetus auratus | 5beta-pregnan-3,20-dione + NADH + H+ | - |
? | |
| alpha-3-hydroxyhexobarbital + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
| beta-3-hydroxyhexobarbital + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
| beta-ionol + NAD+ | - |
Mesocricetus auratus | (3E)-4-(2,6,6-trimethylcyclohex-1-en-1-yl)but-3-en-2-one + NADH + H+ | - |
? | |
| cis-benzene dihydrodiol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
| cyclohexanol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
| dehydroepiandrosterone + NADH + H+ | - |
Mesocricetus auratus | ? + NAD+ | - |
? | |
| farnesol + NAD+ | - |
Mesocricetus auratus | farnesal + NADH + H+ | - |
? | |
| geraniol + NAD+ | - |
Mesocricetus auratus | geranial + NADH + H+ | - |
? | |
| geranylgeraniol + NAD+ | - |
Mesocricetus auratus | geranylgeranial + NADH + H+ | - |
? | |
| additional information | the enzyme AKR1C35 efficiently oxidizes various 3beta- and 17beta-hydroxysteroids, including biologically active 3beta-hydroxy-5alpha/beta-dihydro-C19/C21-steroids, dehydroepiandrosterone, and 17beta-estradiol, substrate specificity, detailed overview. Val54 plays a critical role in non-/recognition of the steroidal substrates. Reaction products identification by thin-layer chromatography (TLC) and liquid chromatography/mass spectrometry (LC/MS). No activity with 5beta-pregnan-3alpha-ol-20-one, 3alpha-hydroxyprogesterone, 5beta-androstan-3alpha-ol-17-one, 20alpha-hydroxyprogesterone, and 5alpha-pregnan-20alpha-ol-3-one | Mesocricetus auratus | ? | - |
? | |
| nerol + NAD+ | - |
Mesocricetus auratus | neral + NADH + H+ | - |
? | |
| testosterone + NAD+ | - |
Mesocricetus auratus | 4-androstenedione + NADH + H+ | - |
? | |
| trans-benzene dihydrodiol + NAD+ | - |
Mesocricetus auratus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, recombinant His-tagged enzyme, SDS-PAGE | Mesocricetus auratus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AKR1C35 | - |
Mesocricetus auratus |
| LOC101842245 | - |
Mesocricetus auratus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Mesocricetus auratus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0028 | - |
5beta-Pregnan-3beta-ol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0043 | - |
5beta-pregnan-3beta,21-diol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0078 | - |
testosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.008 | - |
geranylgeraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.009 | - |
Cyclohexanol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.01 | - |
dehydroepiandrosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.012 | - |
5beta-androstane-3alpha,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.013 | - |
5alpha-pregnan-3beta-ol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.018 | - |
farnesol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.018 | - |
alpha-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.022 | - |
17beta-estradiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.022 | - |
1-Acenaphthenol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.022 | - |
5beta-androstan-3beta-ol-17-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.022 | - |
beta-ionol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.022 | - |
(R)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.023 | - |
geraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.023 | - |
nerol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.025 | - |
cis-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.025 | - |
(S)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.025 | - |
beta-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.027 | - |
5alpha-Androstan-3beta-ol-17-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.028 | - |
(S)-1-indanol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.032 | - |
5beta-dihydrotestosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.033 | - |
trans-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.036 | - |
5beta-androstane-3beta,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.04 | - |
2-Cyclohexen-1-ol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.043 | - |
5-androstene-3beta,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.043 | - |
4-androstene-3alpha,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.056 | - |
3beta-hydroxyprogesterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.057 | - |
5alpha-dihydrotestosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.1 | - |
NADH | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus | |
| 0.11 | - |
NAD+ | pH 7.4, 25°C, with substrate S-tetralol | Mesocricetus auratus | |
| 0.15 | - |
NADP+ | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
substrate oxidation | Mesocricetus auratus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme shows high preference for NAD+ compared with NADP+ | Mesocricetus auratus | |
| NAD+ | conserved residues Gln270 and Glu276 are critical residues for the NAD+ specificity | Mesocricetus auratus | |
| NADH | - |
Mesocricetus auratus | |
| NADP+ | - |
Mesocricetus auratus | |
| NADPH | - |
Mesocricetus auratus | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00011 | - |
chrysin | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.00011 | - |
7-hydroxyflavone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.00039 | - |
lithocholic acid | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.00046 | - |
quercetin | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.00054 | - |
Hexestrol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.00055 | - |
genistein | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.00069 | - |
galangin | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.0033 | - |
zearalenone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.00092 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | 7-hydroxyflavone | |
| 0.001 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | chrysin | |
| 0.0026 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | quercetin | |
| 0.0042 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | genistein | |
| 0.0044 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | lithocholic acid | |
| 0.005 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | galangin | |
| 0.0065 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | Hexestrol | |
| 0.015 | - |
pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | zearalenone | |
| 0.05 | - |
above, pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | Phenolphthalein | |
| 0.05 | - |
above, pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | hinokitiol | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | enzyme AKR1C35 is a member of the aldoketo reductase (AKR) 1C subfamily in the AKR superfamily | Mesocricetus auratus |
| malfunction | mutation of Val54Ala, but not Cys310Phe, significantly impairs the 3beta-hydroxysteroid dehydrogenase enzyme activity, suggesting that Val54 plays a critical role in recognition of the steroidal substrate | Mesocricetus auratus |
| physiological function | enzyme AKR1C35 oxidizes various xenobiotic alicyclic alcohols using NAD+ | Mesocricetus auratus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.063 | - |
NADP+ | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus | |
| 0.117 | - |
Cyclohexanol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.28 | - |
5beta-pregnan-3beta,21-diol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.42 | - |
5beta-Pregnan-3beta-ol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.47 | - |
5beta-androstan-3beta-ol-17-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.6 | - |
5beta-dihydrotestosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.63 | - |
beta-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.78 | - |
(R)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 0.95 | - |
1-Acenaphthenol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 1.3 | - |
alpha-3-hydroxyhexobarbital | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 1.55 | - |
2-Cyclohexen-1-ol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 2.17 | - |
nerol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 2.33 | - |
dehydroepiandrosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 2.5 | - |
cis-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 2.5 | - |
5beta-androstane-3beta,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 2.83 | - |
5alpha-dihydrotestosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 3 | - |
trans-benzene dihydrodiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 3 | - |
5beta-androstane-3alpha,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 3.33 | - |
5alpha-pregnan-3beta-ol-20-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 3.5 | - |
testosterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 5 | - |
NAD+ | pH 7.4, 25°C, with substrate S-tetralol | Mesocricetus auratus | |
| 5.5 | - |
4-androstene-3alpha,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 5.67 | - |
geraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 6 | - |
NADH | pH 7.4, 25°C, with substrate S-camphorquinone | Mesocricetus auratus | |
| 7.67 | - |
geranylgeraniol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 9.67 | - |
5-androstene-3beta,17beta-diol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 10.67 | - |
5alpha-Androstan-3beta-ol-17-one | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 14.17 | - |
farnesol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 16.67 | - |
(S)-1,2,3,4-tetrahydro-1-naphthol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 21.67 | - |
beta-ionol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 23.3 | - |
(S)-1-indanol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 63.33 | - |
3beta-hydroxyprogesterone | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
| 116.7 | - |
17beta-estradiol | pH 7.4, 25°C, recombinant enzyme | Mesocricetus auratus | |
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Release 2023.1 (January 2023)
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