BRENDA - Enzyme Database show
show all sequences of 1.1.1.51

Conformational stability of 17 beta-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus

Ulrih, N.P.; Lanisnik Rizner, T.; FEBS J. 273, 3927-3937 (2006)

Data extracted from this reference:

General Stability
General Stability
Organism
unfolding through 0.4 M guanidine hydrochloride
Curvularia lunata
unfolding through 1.2 M urea
Curvularia lunata
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
NaCl
addition of a neutral electrolyte, such as NaCl, shields repulsion interactions in the highly positively charged enzyme at pH 2.0, effects on the secondary structure by increasing NaCl concentrations at pH 2.0 and 25C, overview
Curvularia lunata
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Curvularia lunata
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-estrene-17beta-ol-3-one + NADP+
-
673553
Curvularia lunata
4-estrene-3,17-dione + NADPH
-
-
-
r
Subunits
Subunits
Commentary
Organism
dimer
secondary structure analysis at 25C and different pH values
Curvularia lunata
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Curvularia lunata
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
additional information
-
binding of the coenzyme NADPH to the enzyme causes local structural changes that do not significantly affect the thermal stability of this protein
Curvularia lunata
60
-
enzyme oligomers are resistant to temperatures up to 60C
Curvularia lunata
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
-
Curvularia lunata
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9
-
Curvularia lunata
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
2
-
25C, 150300 mM NaCl, formation of soluble aggregates enriched in alpha-helical and beta-sheet structures, the soluble aggregates start to precipitate at higher temperatures and NaCl concentrations
Curvularia lunata
7
9
the native dimeric enzyme is thermally stable up to 40C in this pH range
Curvularia lunata
Cofactor
Cofactor
Commentary
Organism
Structure
NADP+
-
Curvularia lunata
NADPH
binding of the coenzyme NADPH to the enzyme causes local structural changes that do not significantly affect the thermal stability of this protein
Curvularia lunata
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADP+
-
Curvularia lunata
NADPH
binding of the coenzyme NADPH to the enzyme causes local structural changes that do not significantly affect the thermal stability of this protein
Curvularia lunata
General Stability (protein specific)
General Stability
Organism
unfolding through 0.4 M guanidine hydrochloride
Curvularia lunata
unfolding through 1.2 M urea
Curvularia lunata
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
NaCl
addition of a neutral electrolyte, such as NaCl, shields repulsion interactions in the highly positively charged enzyme at pH 2.0, effects on the secondary structure by increasing NaCl concentrations at pH 2.0 and 25C, overview
Curvularia lunata
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-estrene-17beta-ol-3-one + NADP+
-
673553
Curvularia lunata
4-estrene-3,17-dione + NADPH
-
-
-
r
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
secondary structure analysis at 25C and different pH values
Curvularia lunata
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Curvularia lunata
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
additional information
-
binding of the coenzyme NADPH to the enzyme causes local structural changes that do not significantly affect the thermal stability of this protein
Curvularia lunata
60
-
enzyme oligomers are resistant to temperatures up to 60C
Curvularia lunata
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
8
-
Curvularia lunata
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9
-
Curvularia lunata
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
2
-
25C, 150300 mM NaCl, formation of soluble aggregates enriched in alpha-helical and beta-sheet structures, the soluble aggregates start to precipitate at higher temperatures and NaCl concentrations
Curvularia lunata
7
9
the native dimeric enzyme is thermally stable up to 40C in this pH range
Curvularia lunata
Other publictions for EC 1.1.1.51
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
725824
Svegelj
The role of Ala231 and Trp227 ...
Curvularia lunata
J. Steroid Biochem. Mol. Biol.
129
92-98
2012
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2
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699146
Bruzzone
Immunohistochemical localizati ...
Pelophylax esculentus
J. Chem. Neuroanat.
39
35-50
2010
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711161
Nashev
The UV-filter benzophenone-1 i ...
Homo sapiens, Mus musculus, Rattus norvegicus
Biochem. Pharmacol.
79
1189-1199
2010
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3
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16
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6
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14
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14
16
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2
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1
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3
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3
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712881
Zhao
Inhibition of 3beta- and 17bet ...
Rattus norvegicus
J. Steroid Biochem. Mol. Biol.
118
13-17
2010
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1
1
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2
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4
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4
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4
1
4
1
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2
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2
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713329
Ernst
Expression of 3beta-HSD and P5 ...
Digitalis lanata
Planta Med.
76
923-927
2010
-
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1
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4
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5
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4
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4
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1
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1
1
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4
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4
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4
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1
1
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696052
Higo
Comparison of sex-steroid synt ...
Rattus norvegicus
Biochem. Biophys. Res. Commun.
385
62-66
2009
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1
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4
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1
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1
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1
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4
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700177
Senthilkumaran
Novel 3beta-hydroxysteroid deh ...
Oreochromis niloticus
Mol. Cell. Endocrinol.
299
146-152
2009
-
-
1
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2
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1
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4
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1
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2
2
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2
-
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4
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700188
Chura
Steroid-converting enzymes in ...
Homo sapiens
Mol. Cell. Endocrinol.
301
51-58
2009
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2
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1
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1
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712925
De Catalfo
Influence of commercial dietar ...
Rattus norvegicus
Lipids
44
345-357
2009
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1
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2
1
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686428
Arukwe
Modulation of steroidogenesis ...
Danio rerio
Environ. Res.
107
362-370
2008
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1
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1
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686929
Prisco
Immunolocalization of 3beta-HS ...
Torpedo marmorata
Gen. Comp. Endocrinol.
155
157-163
2008
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3
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3
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3
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690081
Parthasarathy
Assessment of in vitro effects ...
Rattus norvegicus
Steroids
73
328-338
2008
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2
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1
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691108
Hunter
Transformation of 5-ene steroi ...
Aspergillus tamarii, Aspergillus tamarii KITA
Biochim. Biophys. Acta
1791
110-117
2008
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5
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6
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1
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1
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6
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684324
Aizawa
Expression of steroidogenic en ...
Rattus norvegicus
Am. J. Physiol.
292
E577-E584
2007
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1
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687324
Chen
Gene expression of 3beta-hydro ...
Sus scrofa
J. Anim. Sci.
85
2457-2463
2007
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688683
Luu-The
Steroid metabolism and profile ...
Homo sapiens
J. Steroid Biochem. Mol. Biol.
107
30-36
2007
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3
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1
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689697
Herl
Delta5-3beta-hydroxysteroid de ...
Digitalis lanata
Planta Med.
73
704-710
2007
-
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1
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8
-
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1
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3
-
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1
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1
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6
1
1
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1
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1
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1
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8
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1
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1
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1
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6
1
1
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1
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1
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670259
Ngatcha
3beta-Alkyl-androsterones as i ...
Homo sapiens
Mol. Cell. Endocrinol.
248
225-232
2006
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5
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1
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1
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1
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1
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1
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5
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1
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1
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670273
Luu-The
Characterization of type 12 17 ...
Homo sapiens
Mol. Endocrinol.
20
437-443
2006
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1
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1
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3
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2
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1
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1
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1
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2
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672586
Fink
Identification of a novel seri ...
Homo sapiens
Bioorg. Med. Chem. Lett.
16
1532-1536
2006
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44
-
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1
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2
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2
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1
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46
-
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1
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46
44
-
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2
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2
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673553
Ulrih
Conformational stability of 17 ...
Curvularia lunata
FEBS J.
273
3927-3937
2006
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2
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1
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2
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1
1
1
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1
1
2
2
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2
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2
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1
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1
1
1
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2
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1
1
2
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675983
Lukacik
Structure and function of huma ...
Homo sapiens
Mol. Cell. Endocrinol.
248
61-71
2006
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1
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3
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1
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5
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4
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