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Literature summary for 1.1.1.51 extracted from

  • Lu, M.L.; Huang, Y.W.; Lin, S.X.
    Purification, reconstitution, and steady-state kinetics of the trans-membrane 17beta-hydroxysteroid dehydrogenase 2 (2002), J. Biol. Chem., 277, 22123-22130.
    View publication on PubMed
Search for more literature for 1.1.1.51

Cloned(Commentary)

Cloned (Comment) Organism
His6-tagged 17beta-HSD2 overexpressed in Sf9 cells Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
beta-octylglucoside strong Homo sapiens
dodecyl-beta-D-maltoside inhibition above 0.5% Homo sapiens
Triton X-100 significant Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00025
-
 dihydrotestosterone pH 7.4, with NAD+ as cofactor Homo sapiens
0.00035
-
 estradiol pH 7.4, with NAD+ as cofactor Homo sapiens
0.00053
-
 20alpha-dihydroprogesterone pH 7.4, with NAD+ as cofactor Homo sapiens
0.00061
-
 testosterone pH 7.4, with NAD+ as cofactor Homo sapiens
0.0053
-
 NADH pH 7.4, reaction with 4-dionetestosterone Homo sapiens
0.0056
-
 NADH pH 7.4, reaction with estronel Homo sapiens
0.11
-
 NAD+ pH 7.4, reaction with estradiol Homo sapiens
0.167
-
 NAD+ pH 7.4, reaction with testosterone Homo sapiens
9.6
-
 NADP+ pH 7.4, reaction with estradiol Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Homo sapiens 16020
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42300
-
 2 * 42300, SDS-PAGE Homo sapiens
90400
-
 glycerol gradient ultracentrifugation Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
His6-tagged 17beta-HSD2 overexpressed in Sf9 cells Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5
-
-
 Homo sapiens

Storage Stability

Storage Stability Organism
4°C, activity in both the homogenate and the supernatant using dodecyl-beta-D-maltoside as detergent can be kept for several days without significant loss of activity Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
20alpha-dihydroprogesterone + NAD+
-
 Homo sapiens 4-pregnen-3alpha,20beta-diol + NADH
-
 r
20alpha-dihydroprogesterone + NADP+
-
 Homo sapiens 4-pregnen-3alpha,20beta-diol + NADPH
-
 r
dihydrotestosterone + NAD+
-
 Homo sapiens 5alpha-androstan-3,17-dione + NADH
-
 r
dihydrotestosterone + NADP+
-
 Homo sapiens 5alpha-androstan-3,17-dione + NADPH
-
 r
estradiol + NAD+
-
 Homo sapiens estrone + NADH
-
 ?
estradiol + NADP+
-
 Homo sapiens estrone + NADPH
-
 ?
testosterone + NAD+
-
 Homo sapiens androst-4-en-3,17-dione + NADH + H+
-
 r
testosterone + NADP+
-
 Homo sapiens androst-4-en-3,17-dione + NADPH
-
 r

Subunits

Subunits Comment Organism
dimer 2 * 42300, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
17beta-HSD2
-
 Homo sapiens
17beta-hydroxysteroid dehydrogenase 2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.27
-
 NADH pH 7.4, reaction with 4-dionetestosterone Homo sapiens
0.33
-
 NADH pH 7.4, reaction with estronel Homo sapiens
0.78
-
 20alpha-dihydroprogesterone pH 7.4, with NAD+ as cofactor Homo sapiens
1.3
-
 NADP+ pH 7.4, reaction with estradiol Homo sapiens
2.4
-
 dihydrotestosterone pH 7.4, with NAD+ as cofactor Homo sapiens
2.9
-
 NAD+ pH 7.4, reaction with testosterone Homo sapiens
3.4
-
 testosterone pH 7.4, with NAD+ as cofactor Homo sapiens
3.9
-
 estradiol pH 7.4, with NAD+ as cofactor Homo sapiens
4.7
-
 NAD+ pH 7.4, reaction with estradiol Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+ prefers NAD+ over NADP+ Homo sapiens
NADH
-
 Homo sapiens
NADP+ prefers NAD+ over NADP+ Homo sapiens
NADPH
-
 Homo sapiens