Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Trypanosoma cruzi |
Protein Variants | Comment | Organism |
---|---|---|
K83A | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Trypanosoma cruzi |
K84A | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Trypanosoma cruzi |
L80G | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Trypanosoma cruzi |
R408A | the mutant shows decreased catalytic efficiency compared to the wild type enzyme | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
(3alpha)-3,21-dihydroxypregnan-20-one | - |
Trypanosoma cruzi | |
16-bromoepiandrosterone | no activity at 0.03 mM | Trypanosoma cruzi | |
epiandrosterone | uncompetitive inhibitor, 12% residual activity at 0.03 mM | Trypanosoma cruzi | |
N-ethyl-N'-[(3alpha)-17-oxoandrostan-3-yl]urea | - |
Trypanosoma cruzi | |
N-[(3alpha)-17-oxoandrostan-3-yl]sulfuric diamide | - |
Trypanosoma cruzi | |
N-[(3alpha)-21-hydroxy-20-oxopregnan-3-yl]sulfuric diamide | - |
Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
NADP+ | wild type enzyme, at pH 7.5 and 25°C | Trypanosoma cruzi | |
0.017 | - |
NADP+ | mutant enzyme K84A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
0.017 | - |
NADP+ | mutant enzyme R408A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
0.021 | - |
NADP+ | mutant enzyme K83A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
0.074 | - |
D-glucose 6-phosphate | mutant enzyme L80G, at pH 7.5 and 25°C | Trypanosoma cruzi | |
0.075 | - |
NADP+ | mutant enzyme L80G, at pH 7.5 and 25°C | Trypanosoma cruzi | |
0.077 | - |
D-glucose 6-phosphate | wild type enzyme, at pH 7.5 and 25°C | Trypanosoma cruzi | |
0.168 | - |
D-glucose 6-phosphate | mutant enzyme R408A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
0.537 | - |
D-glucose 6-phosphate | mutant enzyme K83A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
0.618 | - |
D-glucose 6-phosphate | mutant enzyme K84A, at pH 7.5 and 25°C | Trypanosoma cruzi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | Trypanosoma cruzi | - |
6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | Q1WBU6 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Trypanosoma cruzi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ | - |
Trypanosoma cruzi | 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G6PDH | - |
Trypanosoma cruzi |
glucose-6-phosphate dehydrogenase | - |
Trypanosoma cruzi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2 | - |
D-glucose 6-phosphate | mutant enzyme L80G, at pH 7.5 and 25°C | Trypanosoma cruzi | |
2.7 | - |
NADP+ | mutant enzyme L80G, at pH 7.5 and 25°C | Trypanosoma cruzi | |
2.8 | - |
D-glucose 6-phosphate | mutant enzyme K83A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
3 | - |
NADP+ | mutant enzyme K83A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
15 | - |
D-glucose 6-phosphate | mutant enzyme R408A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
19 | - |
NADP+ | mutant enzyme R408A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
24.6 | - |
D-glucose 6-phosphate | mutant enzyme K84A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
52 | - |
NADP+ | wild type enzyme, at pH 7.5 and 25°C | Trypanosoma cruzi | |
58 | - |
NADP+ | mutant enzyme K84A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
62 | - |
D-glucose 6-phosphate | wild type enzyme, at pH 7.5 and 25°C | Trypanosoma cruzi |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
epiandrosterone | wild type enzyme, at pH 7.5 and 25°C | Trypanosoma cruzi | |
0.003 | - |
epiandrosterone | mutant enzyme R408A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
0.008 | - |
epiandrosterone | mutant enzyme K84A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
0.011 | - |
epiandrosterone | mutant enzyme K83A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
0.031 | - |
epiandrosterone | mutant enzyme L80G, at pH 7.5 and 25°C | Trypanosoma cruzi |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000015 | - |
wild type enzyme, at pH 7.5 and 25°C | Trypanosoma cruzi | 16-bromoepiandrosterone | |
0.0015 | - |
wild type enzyme, at pH 7.5 and 25° | Trypanosoma cruzi | N-ethyl-N'-[(3alpha)-17-oxoandrostan-3-yl]urea | |
0.0022 | - |
wild type enzyme, at pH 7.5 and 25° | Trypanosoma cruzi | N-[(3alpha)-17-oxoandrostan-3-yl]sulfuric diamide | |
0.003 | - |
wild type enzyme, at pH 7.5 and 25°C | Trypanosoma cruzi | epiandrosterone |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
D-glucose 6-phosphate | mutant enzyme K83A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
27 | - |
D-glucose 6-phosphate | mutant enzyme L80G, at pH 7.5 and 25°C | Trypanosoma cruzi | |
36 | - |
NADP+ | mutant enzyme L80G, at pH 7.5 and 25°C | Trypanosoma cruzi | |
40 | - |
D-glucose 6-phosphate | mutant enzyme K84A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
89 | - |
D-glucose 6-phosphate | mutant enzyme R408A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
140 | - |
NADP+ | mutant enzyme K83A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
800 | - |
D-glucose 6-phosphate | wild type enzyme, at pH 7.5 and 25°C | Trypanosoma cruzi | |
1100 | - |
NADP+ | mutant enzyme R408A, at pH 7.5 and 25°C | Trypanosoma cruzi | |
3200 | - |
NADP+ | wild type enzyme, at pH 7.5 and 25°C | Trypanosoma cruzi | |
3400 | - |
NADP+ | mutant enzyme K84A, at pH 7.5 and 25°C | Trypanosoma cruzi |