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Literature summary for 1.1.1.49 extracted from
- Molecular cloning, sequence analysis and expression in Escherichia coli of Camelus dromedarius glucose-6-phosphate dehydrogenase cDNA (2012), Protein Expr. Purif., 83, 190-197.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Camelus dromedarius |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Camelus dromedarius |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
x * 63000, SDS-PAGE | Camelus dromedarius |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADP+ | Camelus dromedarius | - |
D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Camelus dromedarius | G1EHI3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged enzyme 95.5fold from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Camelus dromedarius |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 289.6 | - |
purified recombinant His-tagged enzyme, pH 7.5, 25°C | Camelus dromedarius |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-glucose 6-phosphate + NADP+ | - |
Camelus dromedarius | D-glucono-1,5-lactone 6-phosphate + NADPH + H+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 63000, SDS-PAGE | Camelus dromedarius |
| More | structure modeling | Camelus dromedarius |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| G6PD | - |
Camelus dromedarius |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Camelus dromedarius |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Camelus dromedarius |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | the enzyme has two NADP+ binding sites, one is the structural NADP+ and the second is the catalytic NADP+. The structural NADP+ domain adopt a classic beta-alpha-beta dinucleotide fold with the fingerprint sequence GASGDLA at the tight turn following the first beta-strand | Camelus dromedarius | |
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