Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli, His-tagged protein | Trypanosoma cruzi |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5alpha-androstan-16-alpha-bromo-3beta-ol-17-one | in vitro cell viability assays shows a LD50 of 20 microM for Trypanosoma cruzi epimastigotes | Trypanosoma cruzi | |
5alpha-Androstan-3beta-ol-17-one | uncompetitive inhibitor | Trypanosoma cruzi | |
5alpha-androsten-16-alpha-bromo-3beta-ol-17-one | potent inhibitor, in vitro cell viability assays shows a LD50 of 12 microM for Trypanosoma cruzi epimastigotes | Trypanosoma cruzi | |
D-glucose 6-phosphate | - |
Trypanosoma cruzi | |
dehydroandrosterone | uncompetitive inhibitor | Trypanosoma cruzi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0225 | - |
NADP+ | pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | |
0.206 | - |
D-glucose 6-phosphate | pH 7.6, temperature not specified in the publication | Trypanosoma cruzi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma cruzi | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ | sequential mechanism | Trypanosoma cruzi |
Source Tissue | Comment | Organism | Textmining |
---|
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-glucose 6-phosphate + NADP+ + H2O | - |
Trypanosoma cruzi | D-gluconate 6-phosphate + NADPH + H+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
57.1 | - |
D-glucose 6-phosphate | pH 7.6, temperature not specified in the publication | Trypanosoma cruzi |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0048 | - |
5alpha-Androstan-3beta-ol-17-one | substrate D-glucose 6-phosphate, pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | |
0.0048 | - |
5alpha-Androstan-3beta-ol-17-one | substrate NADP+, pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | |
0.0215 | - |
dehydroandrosterone | substrate D-glucose 6-phosphate, pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | |
0.0223 | - |
dehydroandrosterone | substrate NADP+, pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | |
0.1053 | - |
D-glucose 6-phosphate | pH 7.6, temperature not specified in the publication | Trypanosoma cruzi |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000086 | - |
pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | 5alpha-androsten-16-alpha-bromo-3beta-ol-17-one | |
0.000216 | - |
pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | 5alpha-androstan-16-alpha-bromo-3beta-ol-17-one | |
0.0056 | - |
pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | dehydroandrosterone | |
0.025 | - |
pH 7.6, temperature not specified in the publication | Trypanosoma cruzi | 5alpha-Androstan-3beta-ol-17-one |